Restriction map for
pFN21AE3288
(Linear) (Six-base) MAPSORT of: KIEE3288.seq Check: 8185 from: 1 to: 2220
KIEE3288.orf
With 182 enzymes: SgfI *
March 22, 2012 16:28 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 729 2220
Size: 729 1491
AatII G_ACGT'C
Cuts at: 0 711 2220
Size: 711 1509
AccI GT'mk_AC
Cuts at: 0 1321 2220
Size: 1321 899
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 743 883 1409 1815 2220
Size: 743 140 526 406 405
Fragments arranged by size:
743 526 406 405 140
AflIII A'CryG_T
Cuts at: 0 501 783 1870 2220
Size: 501 282 1087 350
Fragments arranged by size:
1087 501 350 282
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 133 2220
Size: 133 2087
AlwNI CAG_nnn'CTG
Cuts at: 0 159 945 2220
Size: 159 786 1275
Fragments arranged by size:
1275 786 159
ApaLI G'TGCA_C
Cuts at: 0 108 2220
Size: 108 2112
ApoI r'AATT_y
Cuts at: 0 555 824 1162 1693 2220
Size: 555 269 338 531 527
Fragments arranged by size:
555 531 527 338 269
AseI AT'TA_AT
Cuts at: 0 484 2220
Size: 484 1736
AvaI C'yCGr_G
Cuts at: 0 72 214 2114 2220
Size: 72 142 1900 106
Fragments arranged by size:
1900 142 106 72
BamHI G'GATC_C
Cuts at: 0 258 2220
Size: 258 1962
BanI G'GyrC_C
Cuts at: 0 77 178 236 2220
Size: 77 101 58 1984
Fragments arranged by size:
1984 101 77 58
BanII G_rGCy'C
Cuts at: 0 582 2220
Size: 582 1638
BbeI G_GCGC'C
Cuts at: 0 81 182 240 2220
Size: 81 101 58 1980
Fragments arranged by size:
1980 101 81 58
BbsI GAAGACnn'nnnn_
Cuts at: 0 39 2220
Size: 39 2181
BbvCI CC'TCA_GC
Cuts at: 0 683 2220
Size: 683 1537
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2095 2129 2220
Size: 2095 34 91
Fragments arranged by size:
2095 91 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 1779 2220
Size: 1779 441
BclI T'GATC_A
Cuts at: 0 936 2220
Size: 936 1284
BglII A'GATC_T
Cuts at: 0 732 1105 2220
Size: 732 373 1115
Fragments arranged by size:
1115 732 373
Bme1580I G_kGCm'C
Cuts at: 0 105 112 1077 2018 2220
Size: 105 7 965 941 202
Fragments arranged by size:
965 941 202 105 7
BmrI ACTGGGnnnn_n'
Cuts at: 0 255 2220
Size: 255 1965
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1365 2220
Size: 1365 855
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 103 2220
Size: 103 2117
Bpu10I CC'TnA_GC
Cuts at: 0 683 2220
Size: 683 1537
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1024 1347 2220
Size: 1024 323 873
Fragments arranged by size:
1024 873 323
BsaHI Gr'CG_yC
Cuts at: 0 78 137 164 179 237 708 2220
Size: 78 59 27 15 58 471 1512
Fragments arranged by size:
1512 471 78 59 58 27 15
BsaWI w'CCGG_w
Cuts at: 0 1037 2220
Size: 1037 1183
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1222 1252 2220
Size: 1222 30 968
Fragments arranged by size:
1222 968 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 37 106 322 2220
Size: 37 69 216 1898
Fragments arranged by size:
1898 216 69 37
BseYI C'CCAG_C
Cuts at: 0 1639 2220
Size: 1639 581
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1420 2089 2220
Size: 1420 669 131
Fragments arranged by size:
1420 669 131
BsiEI CG_ry'CG
Cuts at: 0 130 134 171 375 2220
Size: 130 4 37 204 1845
Fragments arranged by size:
1845 204 130 37 4
BsiHKAI G_wGCw'C
Cuts at: 0 112 2220
Size: 112 2108
Bsp1286I G_dGCh'C
Cuts at: 0 105 112 582 1077 2018 2220
Size: 105 7 470 495 941 202
Fragments arranged by size:
941 495 470 202 105 7
BspHI T'CATG_A
Cuts at: 0 1673 2220
Size: 1673 547
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 729 2220
Size: 729 1491
BsrBI CCG'CTC
Cuts at: 0 71 1211 2220
Size: 71 1140 1009
Fragments arranged by size:
1140 1009 71
BsrDI GCAATG_nn'
Cuts at: 0 563 2220
Size: 563 1657
BsrFI r'CCGG_y
Cuts at: 0 129 2220
Size: 129 2091
BssSI C'ACGA_G
Cuts at: 0 351 2220
Size: 351 1869
BstEII G'GTnAC_C
Cuts at: 0 741 2220
Size: 741 1479
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1950 2220
Size: 1950 270
BstYI r'GATC_y
Cuts at: 0 258 732 960 1105 1332 2152 2220
Size: 258 474 228 145 227 820 68
Fragments arranged by size:
820 474 258 228 227 145 68
BtgI C'CryG_G
Cuts at: 0 225 2220
Size: 225 1995
BtsI GCAGTG_nn'
Cuts at: 0 1658 1979 2220
Size: 1658 321 241
Fragments arranged by size:
1658 321 241
DraIII CAC_nnn'GTG
Cuts at: 0 353 1398 2220
Size: 353 1045 822
Fragments arranged by size:
1045 822 353
EaeI y'GGCC_r
Cuts at: 0 127 131 344 372 560 2033 2220
Size: 127 4 213 28 188 1473 187
Fragments arranged by size:
1473 213 188 187 127 28 4
EagI C'GGCC_G
Cuts at: 0 127 131 372 2220
Size: 127 4 241 1848
Fragments arranged by size:
1848 241 127 4
EarI CTCTTCn'nnn_
Cuts at: 0 428 568 611 1218 1451 2220
Size: 428 140 43 607 233 769
Fragments arranged by size:
769 607 428 233 140 43
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 22 2220
Size: 22 2198
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 103 743 883 1409 1815 2220
Size: 103 640 140 526 406 405
Fragments arranged by size:
640 526 406 405 140 103
EcoO109I rG'GnC_Cy
Cuts at: 0 218 250 2220
Size: 218 32 1970
Fragments arranged by size:
1970 218 32
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1845 2220
Size: 1845 375
FseI GG_CCGG'CC
Cuts at: 0 133 2220
Size: 133 2087
HaeII r_GCGC'y
Cuts at: 0 81 182 240 343 1715 2220
Size: 81 101 58 103 1372 505
Fragments arranged by size:
1372 505 103 101 81 58
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1189 1221 1222 1254 2137 2169 2220
Size: 1189 32 1 32 883 32 51
Fragments arranged by size:
1189 883 51 32 32 32 1
HincII GTy'rAC
Cuts at: 0 1284 2220
Size: 1284 936
HindIII A'AGCT_T
Cuts at: 0 1881 2220
Size: 1881 339
KasI G'GCGC_C
Cuts at: 0 77 178 236 2220
Size: 77 101 58 1984
Fragments arranged by size:
1984 101 77 58
MluI A'CGCG_T
Cuts at: 0 783 2220
Size: 783 1437
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 593 2220
Size: 593 1627
MscI TGG'CCA
Cuts at: 0 346 562 2035 2220
Size: 346 216 1473 185
Fragments arranged by size:
1473 346 216 185
MslI CAynn'nnrTG
Cuts at: 0 547 568 2220
Size: 547 21 1652
Fragments arranged by size:
1652 547 21
MspA1I CmG'CkG
Cuts at: 0 156 227 1313 1792 2220
Size: 156 71 1086 479 428
Fragments arranged by size:
1086 479 428 156 71
NaeI GCC'GGC
Cuts at: 0 131 2220
Size: 131 2089
NarI GG'CG_CC
Cuts at: 0 78 179 237 2220
Size: 78 101 58 1983
Fragments arranged by size:
1983 101 78 58
NdeI CA'TA_TG
Cuts at: 0 495 2220
Size: 495 1725
NgoMIV G'CCGG_C
Cuts at: 0 129 2220
Size: 129 2091
NspI r_CATG'y
Cuts at: 0 505 1874 2220
Size: 505 1369 346
Fragments arranged by size:
1369 505 346
PasI CC'CwG_GG
Cuts at: 0 1719 2011 2220
Size: 1719 292 209
Fragments arranged by size:
1719 292 209
PciI A'CATG_T
Cuts at: 0 501 1870 2220
Size: 501 1369 350
Fragments arranged by size:
1369 501 350
PflMI CCAn_nnn'nTGG
Cuts at: 0 159 2220
Size: 159 2061
PfoI T'CCnGG_A
Cuts at: 0 191 2220
Size: 191 2029
PpuMI rG'GwC_Cy
Cuts at: 0 218 250 2220
Size: 218 32 1970
Fragments arranged by size:
1970 218 32
PshAI GACnn'nnGTC
Cuts at: 0 248 2220
Size: 248 1972
PsiI TTA'TAA
Cuts at: 0 508 2220
Size: 508 1712
PstI C_TGCA'G
Cuts at: 0 648 740 1265 1653 1778 2220
Size: 648 92 525 388 125 442
Fragments arranged by size:
648 525 442 388 125 92
PvuII CAG'CTG
Cuts at: 0 1792 2220
Size: 1792 428
SacII CC_GC'GG
Cuts at: 0 228 2220
Size: 228 1992
SanDI GG'GwC_CC
Cuts at: 0 218 2220
Size: 218 2002
SapI GCTCTTCn'nnn_
Cuts at: 0 428 1218 2220
Size: 428 790 1002
Fragments arranged by size:
1002 790 428
SexAI A'CCwGG_T
Cuts at: 0 1580 2220
Size: 1580 640
SfcI C'TryA_G
Cuts at: 0 644 736 1261 1649 1774 1988 2220
Size: 644 92 525 388 125 214 232
Fragments arranged by size:
644 525 388 232 214 125 92
SfoI GGC'GCC
Cuts at: 0 79 180 238 2220
Size: 79 101 58 1982
Fragments arranged by size:
1982 101 79 58
SmlI C'TyrA_G
Cuts at: 0 1003 1326 2114 2220
Size: 1003 323 788 106
Fragments arranged by size:
1003 788 323 106
StyI C'CwwG_G
Cuts at: 0 1506 2220
Size: 1506 714
TatI w'GTAC_w
Cuts at: 0 1632 2220
Size: 1632 588
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1260 1292 1422 1454 2220
Size: 1260 32 130 32 766
Fragments arranged by size:
1260 766 130 32 32
XbaI T'CTAG_A
Cuts at: 0 1147 2220
Size: 1147 1073
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 400 2220
Size: 400 1820
XhoI C'TCGA_G
Cuts at: 0 2114 2220
Size: 2114 106
ZraI GAC'GTC
Cuts at: 0 709 2220
Size: 709 1511
Enzymes that do cut:
AarI AatII AccI AcuI AflIII AlfI AlwNI ApaLI
ApoI AseI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglII Bme1580I BmrI BplI
BpmI Bpu10I BpuEI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI Bsp1286I BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstEII BstXI BstYI BtgI BtsI DraIII
EaeI EagI EarI EciI Eco57MI EcoO109I FalI FseI
HaeII Hin4I HincII HindIII KasI MluI MmeI MscI
MslI MspA1I NaeI NarI NdeI NgoMIV NspI PasI
PciI PflMI PfoI PpuMI PshAI PsiI PstI PvuII
SacII SanDI SapI SexAI SfcI SfoI SmlI StyI
TatI TstI XbaI XcmI XhoI ZraI
Enzymes that do not cut:
Acc65I AclI AfeI AflII AgeI AhdI AleI AloI
ApaI AscI AsiSI AvrII BaeI BfrBI BglI BlpI
BmgBI BmtI BsaI BsaAI BsaBI BsiWI BsmI BsmBI
BspEI BsrGI BssHII BstAPI BstBI BstZ17I Bsu36I BtgZI
ClaI CspCI DraI DrdI EcoICRI EcoNI EcoRI EcoRV
FspI FspAI HpaI KpnI MfeI NcoI NheI NotI
NruI NsiI PacI PmeI PmlI PpiI PspOMI PspXI
PsrI PvuI RsrII SacI SalI SbfI ScaI SfiI
SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI
StuI SwaI TaqII Tth111I XmaI XmnI