Restriction map for
pFN21AE3287
(Linear) (Six-base) MAPSORT of: KIEE3287.seq Check: 6214 from: 1 to: 2220
KIEE3287.orf
With 182 enzymes: SgfI *
December 27, 2011 16:34 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 20 2220
Size: 20 2200
Acc65I G'GTAC_C
Cuts at: 0 258 2220
Size: 258 1962
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 934 1094 1384 2220
Size: 934 160 290 836
Fragments arranged by size:
934 836 290 160
AflIII A'CryG_T
Cuts at: 0 2184 2220
Size: 2184 36
AleI CACnn'nnGTG
Cuts at: 0 1897 2220
Size: 1897 323
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1316 2220
Size: 1316 904
AlwNI CAG_nnn'CTG
Cuts at: 0 576 2220
Size: 576 1644
ApaLI G'TGCA_C
Cuts at: 0 120 2220
Size: 120 2100
ApoI r'AATT_y
Cuts at: 0 270 299 889 958 1738 2220
Size: 270 29 590 69 780 482
Fragments arranged by size:
780 590 482 270 69 29
AvaI C'yCGr_G
Cuts at: 0 59 105 150 2220
Size: 59 46 45 2070
Fragments arranged by size:
2070 59 46 45
BanI G'GyrC_C
Cuts at: 0 154 258 1891 2220
Size: 154 104 1633 329
Fragments arranged by size:
1633 329 154 104
BanII G_rGCy'C
Cuts at: 0 97 1633 2220
Size: 97 1536 587
Fragments arranged by size:
1536 587 97
BbsI GAAGACnn'nnnn_
Cuts at: 0 1529 2220
Size: 1529 691
BfrBI ATG'CAT
Cuts at: 0 348 2220
Size: 348 1872
BglII A'GATC_T
Cuts at: 0 398 1281 2220
Size: 398 883 939
Fragments arranged by size:
939 883 398
Bme1580I G_kGCm'C
Cuts at: 0 124 157 248 478 1149 2220
Size: 124 33 91 230 671 1071
Fragments arranged by size:
1071 671 230 124 91 33
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 97 2220
Size: 97 2123
Bpu10I CC'TnA_GC
Cuts at: 0 188 2220
Size: 188 2032
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 825 1609 2220
Size: 825 784 611
Fragments arranged by size:
825 784 611
BsaI GGTCTCn'nnnn_
Cuts at: 0 285 2220
Size: 285 1935
BsaBI GATnn'nnATC
Cuts at: 0 347 2220
Size: 347 1873
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 184 214 424 454 2220
Size: 184 30 210 30 1766
Fragments arranged by size:
1766 210 184 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 22 1541 2209 2220
Size: 22 1519 668 11
Fragments arranged by size:
1519 668 22 11
BsiHKAI G_wGCw'C
Cuts at: 0 97 124 218 2220
Size: 97 27 94 2002
Fragments arranged by size:
2002 97 94 27
BsmI GAATG_Cn'
Cuts at: 0 1250 2220
Size: 1250 970
Bsp1286I G_dGCh'C
Cuts at: 0 97 124 157 218 248 478 1149 1633
Size: 97 27 33 61 30 230 671 484
Cuts at: 1633 2220
Size: 587
Fragments arranged by size:
671 587 484 230 97 61 33 30
27
BspHI T'CATG_A
Cuts at: 0 1569 2220
Size: 1569 651
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 20 1255 1266 2220
Size: 20 1235 11 954
Fragments arranged by size:
1235 954 20 11
BsrBI CCG'CTC
Cuts at: 0 30 2220
Size: 30 2190
BsrGI T'GTAC_A
Cuts at: 0 44 1440 2220
Size: 44 1396 780
Fragments arranged by size:
1396 780 44
BssSI C'ACGA_G
Cuts at: 0 34 288 869 2220
Size: 34 254 581 1351
Fragments arranged by size:
1351 581 254 34
BstEII G'GTnAC_C
Cuts at: 0 853 2220
Size: 853 1367
BstXI CCAn_nnnn'nTGG
Cuts at: 0 255 2220
Size: 255 1965
BstYI r'GATC_y
Cuts at: 0 398 1281 2220
Size: 398 883 939
Fragments arranged by size:
939 883 398
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1610 2220
Size: 1610 610
ClaI AT'CG_AT
Cuts at: 0 1313 2220
Size: 1313 907
DraI TTT'AAA
Cuts at: 0 738 2220
Size: 738 1482
DraIII CAC_nnn'GTG
Cuts at: 0 473 1449 2220
Size: 473 976 771
Fragments arranged by size:
976 771 473
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1607 2220
Size: 1607 613
EcoICRI GAG'CTC
Cuts at: 0 95 2220
Size: 95 2125
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 97 934 1094 1384 2220
Size: 97 837 160 290 836
Fragments arranged by size:
837 836 290 160 97
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1000 1498 2220
Size: 1000 498 722
Fragments arranged by size:
1000 722 498
EcoO109I rG'GnC_Cy
Cuts at: 0 1034 2036 2220
Size: 1034 1002 184
Fragments arranged by size:
1034 1002 184
EcoRI G'AATT_C
Cuts at: 0 1738 2220
Size: 1738 482
EcoRV GAT'ATC
Cuts at: 0 1187 2220
Size: 1187 1033
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 823 855 2220
Size: 823 32 1365
Fragments arranged by size:
1365 823 32
HincII GTy'rAC
Cuts at: 0 231 2220
Size: 231 1989
HindIII A'AGCT_T
Cuts at: 0 1076 2220
Size: 1076 1144
KpnI G_GTAC'C
Cuts at: 0 262 2220
Size: 262 1958
MslI CAynn'nnrTG
Cuts at: 0 353 1298 1436 1690 1897 2220
Size: 353 945 138 254 207 323
Fragments arranged by size:
945 353 323 254 207 138
MspA1I CmG'CkG
Cuts at: 0 418 653 1054 2220
Size: 418 235 401 1166
Fragments arranged by size:
1166 418 401 235
NsiI A_TGCA'T
Cuts at: 0 350 2220
Size: 350 1870
NspI r_CATG'y
Cuts at: 0 1297 1330 1796 2220
Size: 1297 33 466 424
Fragments arranged by size:
1297 466 424 33
PfoI T'CCnGG_A
Cuts at: 0 575 800 2220
Size: 575 225 1420
Fragments arranged by size:
1420 575 225
PpuMI rG'GwC_Cy
Cuts at: 0 1034 2220
Size: 1034 1186
PsiI TTA'TAA
Cuts at: 0 1000 2220
Size: 1000 1220
PvuII CAG'CTG
Cuts at: 0 418 653 1054 2220
Size: 418 235 401 1166
Fragments arranged by size:
1166 418 401 235
SacI G_AGCT'C
Cuts at: 0 97 2220
Size: 97 2123
SanDI GG'GwC_CC
Cuts at: 0 1034 2220
Size: 1034 1186
SfcI C'TryA_G
Cuts at: 0 1177 2220
Size: 1177 1043
SmaI CCC'GGG
Cuts at: 0 152 2220
Size: 152 2068
SmlI C'TyrA_G
Cuts at: 0 840 1624 2220
Size: 840 784 596
Fragments arranged by size:
840 784 596
SphI G_CATG'C
Cuts at: 0 1297 2220
Size: 1297 923
StuI AGG'CCT
Cuts at: 0 1648 2220
Size: 1648 572
StyI C'CwwG_G
Cuts at: 0 680 1029 1265 2220
Size: 680 349 236 955
Fragments arranged by size:
955 680 349 236
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 148 1958 2220
Size: 148 1810 262
Fragments arranged by size:
1810 262 148
TatI w'GTAC_w
Cuts at: 0 44 1440 2220
Size: 44 1396 780
Fragments arranged by size:
1396 780 44
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2113 2145 2220
Size: 2113 32 75
Fragments arranged by size:
2113 75 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1804 2220
Size: 1804 416
XmaI C'CCGG_G
Cuts at: 0 150 2220
Size: 150 2070
XmnI GAAnn'nnTTC
Cuts at: 0 1536 2220
Size: 1536 684
Enzymes that do cut:
AarI Acc65I AcuI AflIII AleI AlfI AlwNI ApaLI
ApoI AvaI BanI BanII BbsI BfrBI BglII Bme1580I
BpmI Bpu10I BpuEI BsaI BsaBI BsaXI BseRI BsiHKAI
BsmI Bsp1286I BspHI BspMI BsrBI BsrGI BssSI BstEII
BstXI BstYI BtgZI ClaI DraI DraIII EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI EcoRV Hin4I HincII HindIII
KpnI MslI MspA1I NsiI NspI PfoI PpuMI PsiI
PvuII SacI SanDI SfcI SmaI SmlI SphI StuI
StyI TaqII TatI TstI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AccI AclI AfeI AflII AgeI AhdI AloI
ApaI AscI AseI AsiSI AvrII BaeI BamHI BbeI
BbvCI BcgI BciVI BclI BglI BlpI BmgBI BmrI
BmtI BplI BsaAI BsaHI BsaWI BseYI BsgI BsiEI
BsiWI BsmBI BspEI BsrDI BsrFI BssHII BstAPI BstBI
BstZ17I Bsu36I BtgI BtsI CspCI DrdI EaeI EagI
EarI FalI FseI FspI FspAI HaeII HpaI KasI
MfeI MluI MmeI MscI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI PacI PasI PciI PflMI
PmeI PmlI PpiI PshAI PspOMI PspXI PsrI PstI
PvuI RsrII SacII SalI SapI SbfI ScaI SexAI
SfiI SfoI SgfI SgrAI SnaBI SpeI SrfI SspI
SwaI Tth111I XbaI XhoI ZraI