(Linear) (Six-base) MAPSORT of: KIEE3282.seq Check: 7713 from: 1 to: 2142 KIEE3282.orf With 182 enzymes: SgfI * July 3, 2012 17:34 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 536 2142 Size: 536 1606 AccI GT'mk_AC Cuts at: 0 833 2142 Size: 833 1309 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1465 2142 Size: 1465 677 AfeI AGC'GCT Cuts at: 0 1159 2142 Size: 1159 983 AflIII A'CryG_T Cuts at: 0 755 2142 Size: 755 1387 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 460 2060 2142 Size: 460 1600 82 Fragments arranged by size: 1600 460 82 AlwNI CAG_nnn'CTG Cuts at: 0 942 1196 1950 2034 2142 Size: 942 254 754 84 108 Fragments arranged by size: 942 754 254 108 84 ApaI G_GGCC'C Cuts at: 0 1239 1625 2142 Size: 1239 386 517 Fragments arranged by size: 1239 517 386 ApoI r'AATT_y Cuts at: 0 570 1288 1776 2142 Size: 570 718 488 366 Fragments arranged by size: 718 570 488 366 AvaI C'yCGr_G Cuts at: 0 1032 1584 1800 2142 Size: 1032 552 216 342 Fragments arranged by size: 1032 552 342 216 BamHI G'GATC_C Cuts at: 0 635 651 909 2142 Size: 635 16 258 1233 Fragments arranged by size: 1233 635 258 16 BanI G'GyrC_C Cuts at: 0 42 874 2142 Size: 42 832 1268 Fragments arranged by size: 1268 832 42 BanII G_rGCy'C Cuts at: 0 26 214 1033 1239 1610 1625 1960 2142 Size: 26 188 819 206 371 15 335 182 Fragments arranged by size: 819 371 335 206 188 182 26 15 BbeI G_GCGC'C Cuts at: 0 46 2142 Size: 46 2096 BbsI GAAGACnn'nnnn_ Cuts at: 0 593 1658 2142 Size: 593 1065 484 Fragments arranged by size: 1065 593 484 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1023 1057 2142 Size: 1023 34 1085 Fragments arranged by size: 1085 1023 34 BclI T'GATC_A Cuts at: 0 1085 2142 Size: 1085 1057 BglI GCCn_nnn'nGGC Cuts at: 0 434 2142 Size: 434 1708 BglII A'GATC_T Cuts at: 0 452 1023 1281 2142 Size: 452 571 258 861 Fragments arranged by size: 861 571 452 258 Bme1580I G_kGCm'C Cuts at: 0 134 430 986 1239 1625 2142 Size: 134 296 556 253 386 517 Fragments arranged by size: 556 517 386 296 253 134 BmgBI CAC'GTC Cuts at: 0 1083 2142 Size: 1083 1059 BmrI ACTGGGnnnn_n' Cuts at: 0 2130 2142 Size: 2130 12 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 493 1174 2080 2142 Size: 493 681 906 62 Fragments arranged by size: 906 681 493 62 BsaI GGTCTCn'nnnn_ Cuts at: 0 1863 2142 Size: 1863 279 BsaAI yAC'GTr Cuts at: 0 243 1710 2142 Size: 243 1467 432 Fragments arranged by size: 1467 432 243 BsaBI GATnn'nnATC Cuts at: 0 1999 2142 Size: 1999 143 BsaHI Gr'CG_yC Cuts at: 0 43 2142 Size: 43 2099 BsaWI w'CCGG_w Cuts at: 0 906 1451 1602 1813 1920 2142 Size: 906 545 151 211 107 222 Fragments arranged by size: 906 545 222 211 151 107 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 1891 1921 2142 Size: 1891 30 221 Fragments arranged by size: 1891 221 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 490 1912 2142 Size: 490 1422 230 Fragments arranged by size: 1422 490 230 BseYI C'CCAG_C Cuts at: 0 201 1062 1927 2142 Size: 201 861 865 215 Fragments arranged by size: 865 861 215 201 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 223 514 674 1030 1321 2142 Size: 223 291 160 356 291 821 Fragments arranged by size: 821 356 291 291 223 160 BsiHKAI G_wGCw'C Cuts at: 0 527 1100 2142 Size: 527 573 1042 Fragments arranged by size: 1042 573 527 Bsp1286I G_dGCh'C Cuts at: 0 26 134 214 430 527 986 1033 1100 Size: 26 108 80 216 97 459 47 67 Cuts at: 1100 1239 1610 1625 1960 2142 Size: 139 371 15 335 182 Fragments arranged by size: 459 371 335 216 182 139 108 97 80 67 47 26 15 BspEI T'CCGG_A Cuts at: 0 1813 2142 Size: 1813 329 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 329 536 1388 2142 Size: 329 207 852 754 Fragments arranged by size: 852 754 329 207 BsrBI CCG'CTC Cuts at: 0 125 141 2142 Size: 125 16 2001 Fragments arranged by size: 2001 125 16 BsrDI GCAATG_nn' Cuts at: 0 1938 2142 Size: 1938 204 BsrFI r'CCGG_y Cuts at: 0 3 36 1982 2142 Size: 3 33 1946 160 Fragments arranged by size: 1946 160 33 3 BssSI C'ACGA_G Cuts at: 0 1277 2142 Size: 1277 865 BstXI CCAn_nnnn'nTGG Cuts at: 0 1368 1536 2142 Size: 1368 168 606 Fragments arranged by size: 1368 606 168 BstYI r'GATC_y Cuts at: 0 338 452 635 651 909 1023 1281 2142 Size: 338 114 183 16 258 114 258 861 Fragments arranged by size: 861 338 258 258 183 114 114 16 Bsu36I CC'TnA_GG Cuts at: 0 808 1401 2142 Size: 808 593 741 Fragments arranged by size: 808 741 593 BtgI C'CryG_G Cuts at: 0 83 1550 2142 Size: 83 1467 592 Fragments arranged by size: 1467 592 83 BtsI GCAGTG_nn' Cuts at: 0 2077 2142 Size: 2077 65 ClaI AT'CG_AT Cuts at: 0 731 2142 Size: 731 1411 DraIII CAC_nnn'GTG Cuts at: 0 668 670 2142 Size: 668 2 1472 Fragments arranged by size: 1472 668 2 DrdI GACnn_nn'nnGTC Cuts at: 0 307 2142 Size: 307 1835 EaeI y'GGCC_r Cuts at: 0 74 263 1124 2117 2142 Size: 74 189 861 993 25 Fragments arranged by size: 993 861 189 74 25 EarI CTCTTCn'nnn_ Cuts at: 0 280 913 2142 Size: 280 633 1229 Fragments arranged by size: 1229 633 280 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 343 2142 Size: 343 1799 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 493 1174 1465 2080 2142 Size: 493 681 291 615 62 Fragments arranged by size: 681 615 493 291 62 EcoNI CCTnn'n_nnAGG Cuts at: 0 1444 2142 Size: 1444 698 EcoO109I rG'GnC_Cy Cuts at: 0 1235 1236 1580 1621 1902 2054 2142 Size: 1235 1 344 41 281 152 88 Fragments arranged by size: 1235 344 281 152 88 41 1 EcoRI G'AATT_C Cuts at: 0 1288 2142 Size: 1288 854 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 1488 2142 Size: 1488 654 FspI TGC'GCA Cuts at: 0 630 2142 Size: 630 1512 HaeII r_GCGC'y Cuts at: 0 46 1161 2025 2142 Size: 46 1115 864 117 Fragments arranged by size: 1115 864 117 46 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 494 526 724 756 1266 1298 2142 Size: 494 32 198 32 510 32 844 Fragments arranged by size: 844 510 494 198 32 32 32 HincII GTy'rAC Cuts at: 0 770 2142 Size: 770 1372 KasI G'GCGC_C Cuts at: 0 42 2142 Size: 42 2100 MfeI C'AATT_G Cuts at: 0 1530 1962 2142 Size: 1530 432 180 Fragments arranged by size: 1530 432 180 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 525 1127 1349 1873 2142 Size: 525 602 222 524 269 Fragments arranged by size: 602 525 524 269 222 MscI TGG'CCA Cuts at: 0 265 1126 2142 Size: 265 861 1016 Fragments arranged by size: 1016 861 265 MslI CAynn'nnrTG Cuts at: 0 748 1564 2142 Size: 748 816 578 Fragments arranged by size: 816 748 578 MspA1I CmG'CkG Cuts at: 0 85 2142 Size: 85 2057 NaeI GCC'GGC Cuts at: 0 5 38 2142 Size: 5 33 2104 Fragments arranged by size: 2104 33 5 NarI GG'CG_CC Cuts at: 0 43 2142 Size: 43 2099 NcoI C'CATG_G Cuts at: 0 1550 2142 Size: 1550 592 NgoMIV G'CCGG_C Cuts at: 0 3 36 2142 Size: 3 33 2106 Fragments arranged by size: 2106 33 3 NruI TCG'CGA Cuts at: 0 1700 2142 Size: 1700 442 NspI r_CATG'y Cuts at: 0 759 1824 2142 Size: 759 1065 318 Fragments arranged by size: 1065 759 318 PasI CC'CwG_GG Cuts at: 0 430 2142 Size: 430 1712 PciI A'CATG_T Cuts at: 0 755 2142 Size: 755 1387 PflMI CCAn_nnn'nTGG Cuts at: 0 828 1122 2142 Size: 828 294 1020 Fragments arranged by size: 1020 828 294 PfoI T'CCnGG_A Cuts at: 0 899 1184 2142 Size: 899 285 958 Fragments arranged by size: 958 899 285 PmlI CAC'GTG Cuts at: 0 243 2142 Size: 243 1899 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1369 1401 2142 Size: 1369 32 741 Fragments arranged by size: 1369 741 32 PpuMI rG'GwC_Cy Cuts at: 0 1580 2142 Size: 1580 562 PspOMI G'GGCC_C Cuts at: 0 1235 1621 2142 Size: 1235 386 521 Fragments arranged by size: 1235 521 386 PstI C_TGCA'G Cuts at: 0 2039 2049 2084 2142 Size: 2039 10 35 58 Fragments arranged by size: 2039 58 35 10 SacII CC_GC'GG Cuts at: 0 86 2142 Size: 86 2056 SexAI A'CCwGG_T Cuts at: 0 410 2142 Size: 410 1732 SfcI C'TryA_G Cuts at: 0 1737 2035 2045 2080 2142 Size: 1737 298 10 35 62 Fragments arranged by size: 1737 298 62 35 10 SfoI GGC'GCC Cuts at: 0 44 2142 Size: 44 2098 SmaI CCC'GGG Cuts at: 0 1802 2142 Size: 1802 340 SspI AAT'ATT Cuts at: 0 930 1506 2142 Size: 930 576 636 Fragments arranged by size: 930 636 576 StuI AGG'CCT Cuts at: 0 1258 1459 2142 Size: 1258 201 683 Fragments arranged by size: 1258 683 201 StyI C'CwwG_G Cuts at: 0 221 1241 1550 2142 Size: 221 1020 309 592 Fragments arranged by size: 1020 592 309 221 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 108 1069 2142 Size: 108 961 1073 Fragments arranged by size: 1073 961 108 TatI w'GTAC_w Cuts at: 0 1229 2142 Size: 1229 913 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1891 1923 2142 Size: 1891 32 219 Fragments arranged by size: 1891 219 32 Tth111I GACn'n_nGTC Cuts at: 0 1794 2142 Size: 1794 348 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 1947 2142 Size: 1947 195 XmaI C'CCGG_G Cuts at: 0 1800 2142 Size: 1800 342 Enzymes that do cut: AarI AccI AcuI AfeI AflIII AlfI AlwNI ApaI ApoI AvaI BamHI BanI BanII BbeI BbsI BcgI BclI BglI BglII Bme1580I BmgBI BmrI BpmI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI Bsp1286I BspEI BspMI BsrBI BsrDI BsrFI BssSI BstXI BstYI Bsu36I BtgI BtsI ClaI DraIII DrdI EaeI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI MfeI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NruI NspI PasI PciI PflMI PfoI PmlI PpiI PpuMI PspOMI PstI SacII SexAI SfcI SfoI SmaI SspI StuI StyI TaqII TatI TstI Tth111I XcmI XmaI Enzymes that do not cut: AatII Acc65I AclI AflII AgeI AhdI AleI AloI ApaLI AscI AseI AsiSI AvrII BaeI BbvCI BciVI BfrBI BlpI BmtI BplI Bpu10I BpuEI BsiEI BsiWI BsmI BsmBI BspHI BsrGI BssHII BstAPI BstBI BstEII BstZ17I BtgZI CspCI DraI EagI EcoICRI EcoRV FseI FspAI HindIII HpaI KpnI MluI NdeI NheI NotI NsiI PacI PmeI PshAI PsiI PspXI PsrI PvuI PvuII RsrII SacI SalI SanDI SapI SbfI ScaI SfiI SgfI SgrAI SmlI SnaBI SpeI SphI SrfI SwaI XbaI XhoI XmnI ZraI