Restriction map for
pFN21AE3272
(Linear) (Six-base) MAPSORT of: KIEE3272.seq Check: 1985 from: 1 to: 2025
KIEE3272.orf
With 182 enzymes: SgfI *
December 27, 2011 16:34 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 156 536 944 953 1073 2025
Size: 156 380 408 9 120 952
Fragments arranged by size:
952 408 380 156 120 9
AatII G_ACGT'C
Cuts at: 0 1433 2025
Size: 1433 592
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 205 256 1884 2025
Size: 205 51 1628 141
Fragments arranged by size:
1628 205 141 51
AfeI AGC'GCT
Cuts at: 0 1843 2025
Size: 1843 182
AflIII A'CryG_T
Cuts at: 0 461 1016 2025
Size: 461 555 1009
Fragments arranged by size:
1009 555 461
AlwNI CAG_nnn'CTG
Cuts at: 0 963 1044 1233 2025
Size: 963 81 189 792
Fragments arranged by size:
963 792 189 81
ApaI G_GGCC'C
Cuts at: 0 10 43 444 1322 1470 1876 1966 2025
Size: 10 33 401 878 148 406 90 59
Fragments arranged by size:
878 406 401 148 90 59 33 10
ApaLI G'TGCA_C
Cuts at: 0 1438 2025
Size: 1438 587
ApoI r'AATT_y
Cuts at: 0 1648 2025
Size: 1648 377
AvaI C'yCGr_G
Cuts at: 0 36 520 1394 1461 1868 1990 2025
Size: 36 484 874 67 407 122 35
Fragments arranged by size:
874 484 407 122 67 36 35
BanI G'GyrC_C
Cuts at: 0 53 172 524 941 1028 1751 2025
Size: 53 119 352 417 87 723 274
Fragments arranged by size:
723 417 352 274 119 87 53
BanII G_rGCy'C
Cuts at: 0 10 35 43 444 1106 1322 1470 1775
Size: 10 25 8 401 662 216 148 305
Cuts at: 1775 1876 1966 1997 2025
Size: 101 90 31 28
Fragments arranged by size:
662 401 305 216 148 101 90 31
28 25 10 8
BbeI G_GCGC'C
Cuts at: 0 57 1032 2025
Size: 57 975 993
Fragments arranged by size:
993 975 57
BbsI GAAGACnn'nnnn_
Cuts at: 0 193 771 826 1466 2025
Size: 193 578 55 640 559
Fragments arranged by size:
640 578 559 193 55
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 355 389 817 851 2025
Size: 355 34 428 34 1174
Fragments arranged by size:
1174 428 355 34 34
BglI GCCn_nnn'nGGC
Cuts at: 0 215 1207 2025
Size: 215 992 818
Fragments arranged by size:
992 818 215
BglII A'GATC_T
Cuts at: 0 1070 2025
Size: 1070 955
BlpI GC'TnA_GC
Cuts at: 0 1234 1925 2025
Size: 1234 691 100
Fragments arranged by size:
1234 691 100
Bme1580I G_kGCm'C
Cuts at: 0 10 43 175 444 527 1322 1442 1470
Size: 10 33 132 269 83 795 120 28
Cuts at: 1470 1754 1876 1966 2025
Size: 284 122 90 59
Fragments arranged by size:
795 284 269 132 122 120 90 83
59 33 28 10
BmgBI CAC'GTC
Cuts at: 0 777 798 2025
Size: 777 21 1227
Fragments arranged by size:
1227 777 21
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 48 118 1017 1291 1618 1809 2025
Size: 48 70 899 274 327 191 216
Fragments arranged by size:
899 327 274 216 191 70 48
Bpu10I CC'TnA_GC
Cuts at: 0 716 844 2025
Size: 716 128 1181
Fragments arranged by size:
1181 716 128
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 331 2025
Size: 331 1694
BsaI GGTCTCn'nnnn_
Cuts at: 0 541 1237 2025
Size: 541 696 788
Fragments arranged by size:
788 696 541
BsaAI yAC'GTr
Cuts at: 0 1017 1443 2025
Size: 1017 426 582
Fragments arranged by size:
1017 582 426
BsaHI Gr'CG_yC
Cuts at: 0 54 76 1029 1430 2025
Size: 54 22 953 401 595
Fragments arranged by size:
953 595 401 54 22
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 410 440 1807 1837 2025
Size: 410 30 1367 30 188
Fragments arranged by size:
1367 410 188 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 759 1365 1401 1498 2025
Size: 759 606 36 97 527
Fragments arranged by size:
759 606 527 97 36
BseYI C'CCAG_C
Cuts at: 0 213 603 1032 1321 1613 1997 2025
Size: 213 390 429 289 292 384 28
Fragments arranged by size:
429 390 384 292 289 213 28
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 184 1939 2025
Size: 184 1755 86
Fragments arranged by size:
1755 184 86
BsiEI CG_ry'CG
Cuts at: 0 26 47 249 612 926 1149 1586 2025
Size: 26 21 202 363 314 223 437 439
Fragments arranged by size:
439 437 363 314 223 202 26 21
BsiHKAI G_wGCw'C
Cuts at: 0 35 245 1106 1145 1442 2025
Size: 35 210 861 39 297 583
Fragments arranged by size:
861 583 297 210 39 35
Bsp1286I G_dGCh'C
Cuts at: 0 10 35 43 175 245 444 527 1106
Size: 10 25 8 132 70 199 83 579
Cuts at: 1106 1145 1322 1442 1470 1754 1775 1876 1966
Size: 39 177 120 28 284 21 101 90
Cuts at: 1966 1997 2025
Size: 31 28
Fragments arranged by size:
579 284 199 177 132 120 101 90
83 70 39 31 28 28 25 21
10 8
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 156 536 944 953 1073 1262 2025
Size: 156 380 408 9 120 189 763
Fragments arranged by size:
763 408 380 189 156 120 9
BsrBI CCG'CTC
Cuts at: 0 1275 2025
Size: 1275 750
BsrDI GCAATG_nn'
Cuts at: 0 710 950 1749 2025
Size: 710 240 799 276
Fragments arranged by size:
799 710 276 240
BsrFI r'CCGG_y
Cuts at: 0 1189 1682 2025
Size: 1189 493 343
Fragments arranged by size:
1189 493 343
BssHII G'CGCG_C
Cuts at: 0 918 2025
Size: 918 1107
BssSI C'ACGA_G
Cuts at: 0 1452 2025
Size: 1452 573
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1140 2025
Size: 1140 885
BstEII G'GTnAC_C
Cuts at: 0 298 2025
Size: 298 1727
BstYI r'GATC_y
Cuts at: 0 1070 1950 2025
Size: 1070 880 75
Fragments arranged by size:
1070 880 75
BtgI C'CryG_G
Cuts at: 0 20 1022 1095 1580 1764 2025
Size: 20 1002 73 485 184 261
Fragments arranged by size:
1002 485 261 184 73 20
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 387 1647 2025
Size: 387 1260 378
Fragments arranged by size:
1260 387 378
BtsI GCAGTG_nn'
Cuts at: 0 863 2011 2025
Size: 863 1148 14
Fragments arranged by size:
1148 863 14
ClaI AT'CG_AT
Cuts at: 0 1832 2025
Size: 1832 193
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 306 341 2025
Size: 306 35 1684
Fragments arranged by size:
1684 306 35
DraIII CAC_nnn'GTG
Cuts at: 0 196 2025
Size: 196 1829
EaeI y'GGCC_r
Cuts at: 0 23 44 246 707 800 923 965 1146
Size: 23 21 202 461 93 123 42 181
Cuts at: 1146 1187 1557 1583 2025
Size: 41 370 26 442
Fragments arranged by size:
461 442 370 202 181 123 93 42
41 26 23 21
EagI C'GGCC_G
Cuts at: 0 23 44 246 923 1146 1583 2025
Size: 23 21 202 677 223 437 442
Fragments arranged by size:
677 442 437 223 202 23 21
EcoICRI GAG'CTC
Cuts at: 0 33 1104 2025
Size: 33 1071 921
Fragments arranged by size:
1071 921 33
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 48 118 205 256 1017 1291 1618 1809
Size: 48 70 87 51 761 274 327 191
Cuts at: 1809 1884 2025
Size: 75 141
Fragments arranged by size:
761 327 274 191 141 87 75 70
51 48
EcoNI CCTnn'n_nnAGG
Cuts at: 0 556 595 2025
Size: 556 39 1430
Fragments arranged by size:
1430 556 39
EcoO109I rG'GnC_Cy
Cuts at: 0 180 351 440 441 516 629 749 1041
Size: 180 171 89 1 75 113 120 292
Cuts at: 1041 1251 1318 1391 1466 1467 1872 1873 1962
Size: 210 67 73 75 1 405 1 89
Cuts at: 1962 2025
Size: 63
Fragments arranged by size:
405 292 210 180 171 120 113 89
89 75 75 73 67 63 1 1
1
EcoRI G'AATT_C
Cuts at: 0 1648 2025
Size: 1648 377
HaeII r_GCGC'y
Cuts at: 0 57 97 256 363 705 792 1032 1427
Size: 57 40 159 107 342 87 240 395
Cuts at: 1427 1845 2025
Size: 418 180
Fragments arranged by size:
418 395 342 240 180 159 107 87
57 40
HincII GTy'rAC
Cuts at: 0 1505 2025
Size: 1505 520
KasI G'GCGC_C
Cuts at: 0 53 1028 2025
Size: 53 975 997
Fragments arranged by size:
997 975 53
MscI TGG'CCA
Cuts at: 0 709 1559 2025
Size: 709 850 466
Fragments arranged by size:
850 709 466
MslI CAynn'nnrTG
Cuts at: 0 269 1120 1742 2025
Size: 269 851 622 283
Fragments arranged by size:
851 622 283 269
MspA1I CmG'CkG
Cuts at: 0 22 213 390 1131 1137 1416 1698 1944
Size: 22 191 177 741 6 279 282 246
Cuts at: 1944 2025
Size: 81
Fragments arranged by size:
741 282 279 246 191 177 81 22
6
NaeI GCC'GGC
Cuts at: 0 1191 1684 2025
Size: 1191 493 341
Fragments arranged by size:
1191 493 341
NarI GG'CG_CC
Cuts at: 0 54 1029 2025
Size: 54 975 996
Fragments arranged by size:
996 975 54
NcoI C'CATG_G
Cuts at: 0 1022 1095 2025
Size: 1022 73 930
Fragments arranged by size:
1022 930 73
NgoMIV G'CCGG_C
Cuts at: 0 1189 1682 2025
Size: 1189 493 343
Fragments arranged by size:
1189 493 343
NspI r_CATG'y
Cuts at: 0 1806 2025
Size: 1806 219
PasI CC'CwG_GG
Cuts at: 0 1203 2025
Size: 1203 822
PflMI CCAn_nnn'nTGG
Cuts at: 0 858 963 2025
Size: 858 105 1062
Fragments arranged by size:
1062 858 105
PfoI T'CCnGG_A
Cuts at: 0 64 490 1825 2025
Size: 64 426 1335 200
Fragments arranged by size:
1335 426 200 64
PmlI CAC'GTG
Cuts at: 0 1443 2025
Size: 1443 582
PpuMI rG'GwC_Cy
Cuts at: 0 180 351 516 1251 2025
Size: 180 171 165 735 774
Fragments arranged by size:
774 735 180 171 165
PspOMI G'GGCC_C
Cuts at: 0 6 39 440 1318 1466 1872 1962 2025
Size: 6 33 401 878 148 406 90 63
Fragments arranged by size:
878 406 401 148 90 63 33 6
PstI C_TGCA'G
Cuts at: 0 908 1070 1136 2025
Size: 908 162 66 889
Fragments arranged by size:
908 889 162 66
PvuII CAG'CTG
Cuts at: 0 213 1131 1137 2025
Size: 213 918 6 888
Fragments arranged by size:
918 888 213 6
SacI G_AGCT'C
Cuts at: 0 35 1106 2025
Size: 35 1071 919
Fragments arranged by size:
1071 919 35
SacII CC_GC'GG
Cuts at: 0 23 2025
Size: 23 2002
ScaI AGT'ACT
Cuts at: 0 811 2025
Size: 811 1214
SfcI C'TryA_G
Cuts at: 0 204 753 876 904 1066 1132 2025
Size: 204 549 123 28 162 66 893
Fragments arranged by size:
893 549 204 162 123 66 28
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1207 2025
Size: 1207 818
SfoI GGC'GCC
Cuts at: 0 55 1030 2025
Size: 55 975 995
Fragments arranged by size:
995 975 55
SmaI CCC'GGG
Cuts at: 0 38 522 1396 1870 2025
Size: 38 484 874 474 155
Fragments arranged by size:
874 484 474 155 38
SmlI C'TyrA_G
Cuts at: 0 346 2025
Size: 346 1679
StuI AGG'CCT
Cuts at: 0 562 871 2025
Size: 562 309 1154
Fragments arranged by size:
1154 562 309
StyI C'CwwG_G
Cuts at: 0 317 1022 1095 2025
Size: 317 705 73 930
Fragments arranged by size:
930 705 317 73
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 667 1657 1750 2025
Size: 667 990 93 275
Fragments arranged by size:
990 667 275 93
TatI w'GTAC_w
Cuts at: 0 809 2025
Size: 809 1216
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 282 314 335 367 1471 1503 2025
Size: 282 32 21 32 1104 32 522
Fragments arranged by size:
1104 522 282 32 32 32 21
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 330 2025
Size: 330 1695
XmaI C'CCGG_G
Cuts at: 0 36 520 1394 1868 2025
Size: 36 484 874 474 157
Fragments arranged by size:
874 484 474 157 36
ZraI GAC'GTC
Cuts at: 0 1431 2025
Size: 1431 594
Enzymes that do cut:
AarI AatII AcuI AfeI AflIII AlwNI ApaI ApaLI
ApoI AvaI BanI BanII BbeI BbsI BcgI BglI
BglII BlpI Bme1580I BmgBI BpmI Bpu10I BpuEI BsaI
BsaAI BsaHI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
Bsp1286I BspMI BsrBI BsrDI BsrFI BssHII BssSI BstAPI
BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraIII
EaeI EagI EcoICRI Eco57MI EcoNI EcoO109I EcoRI HaeII
HincII KasI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PasI PflMI PfoI PmlI PpuMI PspOMI
PstI PvuII SacI SacII ScaI SfcI SfiI SfoI
SmaI SmlI StuI StyI TaqII TatI TstI XcmI
XmaI ZraI
Enzymes that do not cut:
AccI Acc65I AclI AflII AgeI AhdI AleI AlfI
AloI AscI AseI AsiSI AvrII BaeI BamHI BbvCI
BciVI BclI BfrBI BmrI BmtI BplI BsaBI BsaWI
BsiWI BsmI BsmBI BspEI BspHI BsrGI BstBI BstXI
BstZ17I Bsu36I DraI DrdI EarI EciI EcoRV FalI
FseI FspI FspAI Hin4I HindIII HpaI KpnI MfeI
MluI MmeI NdeI NheI NotI NruI NsiI PacI
PciI PmeI PpiI PshAI PsiI PspXI PsrI PvuI
RsrII SalI SanDI SapI SbfI SexAI SgfI SgrAI
SnaBI SpeI SphI SrfI SspI SwaI Tth111I XbaI
XhoI XmnI