Restriction map for
pFN21AE3267
(Linear) (Six-base) MAPSORT of: KIEE3267.seq Check: 910 from: 1 to: 1986
KIEE3267.orf
With 182 enzymes: SgfI *
July 3, 2012 17:33 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1124 1986
Size: 1124 862
ApoI r'AATT_y
Cuts at: 0 407 1204 1986
Size: 407 797 782
Fragments arranged by size:
797 782 407
AseI AT'TA_AT
Cuts at: 0 7 1986
Size: 7 1979
BamHI G'GATC_C
Cuts at: 0 570 1098 1986
Size: 570 528 888
Fragments arranged by size:
888 570 528
BanI G'GyrC_C
Cuts at: 0 1034 1986
Size: 1034 952
BanII G_rGCy'C
Cuts at: 0 63 509 1986
Size: 63 446 1477
Fragments arranged by size:
1477 446 63
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 945 979 1986
Size: 945 34 1007
Fragments arranged by size:
1007 945 34
BclI T'GATC_A
Cuts at: 0 435 918 1986
Size: 435 483 1068
Fragments arranged by size:
1068 483 435
BfrBI ATG'CAT
Cuts at: 0 54 1986
Size: 54 1932
Bme1580I G_kGCm'C
Cuts at: 0 127 1037 1473 1986
Size: 127 910 436 513
Fragments arranged by size:
910 513 436 127
BmgBI CAC'GTC
Cuts at: 0 1921 1986
Size: 1921 65
BmrI ACTGGGnnnn_n'
Cuts at: 0 1834 1986
Size: 1834 152
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 481 1603 1986
Size: 481 1122 383
Fragments arranged by size:
1122 481 383
Bpu10I CC'TnA_GC
Cuts at: 0 149 1000 1986
Size: 149 851 986
Fragments arranged by size:
986 851 149
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 36 230 1986
Size: 36 194 1756
Fragments arranged by size:
1756 194 36
BsaI GGTCTCn'nnnn_
Cuts at: 0 1579 1986
Size: 1579 407
BsaBI GATnn'nnATC
Cuts at: 0 434 1986
Size: 434 1552
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 106 136 1986
Size: 106 30 1850
Fragments arranged by size:
1850 106 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 304 1009 1986
Size: 304 705 977
Fragments arranged by size:
977 705 304
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 667 1986
Size: 667 1319
BsiHKAI G_wGCw'C
Cuts at: 0 63 1986
Size: 63 1923
BsmI GAATG_Cn'
Cuts at: 0 613 1381 1513 1986
Size: 613 768 132 473
Fragments arranged by size:
768 613 473 132
BsmBI CGTCTCn'nnnn_
Cuts at: 0 625 1986
Size: 625 1361
Bsp1286I G_dGCh'C
Cuts at: 0 63 127 509 1037 1473 1986
Size: 63 64 382 528 436 513
Fragments arranged by size:
528 513 436 382 64 63
BspHI T'CATG_A
Cuts at: 0 1893 1986
Size: 1893 93
BstBI TT'CG_AA
Cuts at: 0 199 1986
Size: 199 1787
BstYI r'GATC_y
Cuts at: 0 570 1098 1883 1986
Size: 570 528 785 103
Fragments arranged by size:
785 570 528 103
BtsI GCAGTG_nn'
Cuts at: 0 679 899 1178 1986
Size: 679 220 279 808
Fragments arranged by size:
808 679 279 220
DraI TTT'AAA
Cuts at: 0 74 830 1986
Size: 74 756 1156
Fragments arranged by size:
1156 756 74
DraIII CAC_nnn'GTG
Cuts at: 0 229 1986
Size: 229 1757
EcoICRI GAG'CTC
Cuts at: 0 61 1986
Size: 61 1925
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 481 1124 1603 1986
Size: 481 643 479 383
Fragments arranged by size:
643 481 479 383
EcoRV GAT'ATC
Cuts at: 0 1758 1986
Size: 1758 228
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1875 1907 1986
Size: 1875 32 79
Fragments arranged by size:
1875 79 32
HincII GTy'rAC
Cuts at: 0 1389 1676 1986
Size: 1389 287 310
Fragments arranged by size:
1389 310 287
HindIII A'AGCT_T
Cuts at: 0 479 1986
Size: 479 1507
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 890 1076 1986
Size: 890 186 910
Fragments arranged by size:
910 890 186
MspA1I CmG'CkG
Cuts at: 0 516 1986
Size: 516 1470
NsiI A_TGCA'T
Cuts at: 0 56 1986
Size: 56 1930
NspI r_CATG'y
Cuts at: 0 568 797 976 1986
Size: 568 229 179 1010
Fragments arranged by size:
1010 568 229 179
PflMI CCAn_nnn'nTGG
Cuts at: 0 229 1986
Size: 229 1757
PfoI T'CCnGG_A
Cuts at: 0 1502 1986
Size: 1502 484
PsiI TTA'TAA
Cuts at: 0 355 718 946 1986
Size: 355 363 228 1040
Fragments arranged by size:
1040 363 355 228
PstI C_TGCA'G
Cuts at: 0 1117 1135 1986
Size: 1117 18 851
Fragments arranged by size:
1117 851 18
PvuII CAG'CTG
Cuts at: 0 516 1986
Size: 516 1470
SacI G_AGCT'C
Cuts at: 0 63 1986
Size: 63 1923
ScaI AGT'ACT
Cuts at: 0 1129 1986
Size: 1129 857
SexAI A'CCwGG_T
Cuts at: 0 596 1986
Size: 596 1390
SfcI C'TryA_G
Cuts at: 0 1113 1131 1855 1986
Size: 1113 18 724 131
Fragments arranged by size:
1113 724 131 18
SmlI C'TyrA_G
Cuts at: 0 15 245 1986
Size: 15 230 1741
Fragments arranged by size:
1741 230 15
SpeI A'CTAG_T
Cuts at: 0 526 1986
Size: 526 1460
SspI AAT'ATT
Cuts at: 0 1406 1936 1986
Size: 1406 530 50
Fragments arranged by size:
1406 530 50
StyI C'CwwG_G
Cuts at: 0 127 1986
Size: 127 1859
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1347 1986
Size: 1347 639
TatI w'GTAC_w
Cuts at: 0 796 1127 1986
Size: 796 331 859
Fragments arranged by size:
859 796 331
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 106 138 1083 1115 1986
Size: 106 32 945 32 871
Fragments arranged by size:
945 871 106 32 32
XmnI GAAnn'nnTTC
Cuts at: 0 1145 1986
Size: 1145 841
Enzymes that do cut:
AcuI ApoI AseI BamHI BanI BanII BcgI BclI
BfrBI Bme1580I BmgBI BmrI BpmI Bpu10I BpuEI BsaI
BsaBI BsaXI BseRI BsgI BsiHKAI BsmI BsmBI Bsp1286I
BspHI BstBI BstYI BtsI DraI DraIII EcoICRI Eco57MI
EcoRV Hin4I HincII HindIII MmeI MspA1I NsiI NspI
PflMI PfoI PsiI PstI PvuII SacI ScaI SexAI
SfcI SmlI SpeI SspI StyI TaqII TatI TstI
XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
AscI AsiSI AvaI AvrII BaeI BbeI BbsI BbvCI
BciVI BglI BglII BlpI BmtI BplI BsaAI BsaHI
BsaWI BseYI BsiEI BsiWI BspEI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstEII BstXI BstZ17I
Bsu36I BtgI BtgZI ClaI CspCI DrdI EaeI EagI
EarI EciI EcoNI EcoO109I EcoRI FalI FseI FspI
FspAI HaeII HpaI KasI KpnI MfeI MluI MscI
MslI NaeI NarI NcoI NdeI NgoMIV NheI NotI
NruI PacI PasI PciI PmeI PmlI PpiI PpuMI
PshAI PspOMI PspXI PsrI PvuI RsrII SacII SalI
SanDI SapI SbfI SfiI SfoI SgfI SgrAI SmaI
SnaBI SphI SrfI StuI SwaI Tth111I XbaI XcmI
XhoI XmaI ZraI