Restriction map for
pFN21AE3264
(Linear) (Six-base) MAPSORT of: KIEE3264.seq Check: 3801 from: 1 to: 1929
KIEE3264.orf
With 182 enzymes: SgfI *
March 22, 2012 16:28 ..
AclI AA'CG_TT
Cuts at: 0 1785 1929
Size: 1785 144
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1826 1894 1929
Size: 1826 68 35
Fragments arranged by size:
1826 68 35
AfeI AGC'GCT
Cuts at: 0 22 1929
Size: 22 1907
AflIII A'CryG_T
Cuts at: 0 833 948 1929
Size: 833 115 981
Fragments arranged by size:
981 833 115
AhdI GACnn_n'nnGTC
Cuts at: 0 1871 1929
Size: 1871 58
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1762 1929
Size: 1762 167
AlwNI CAG_nnn'CTG
Cuts at: 0 228 1929
Size: 228 1701
ApaI G_GGCC'C
Cuts at: 0 210 1929
Size: 210 1719
ApoI r'AATT_y
Cuts at: 0 361 369 923 1203 1929
Size: 361 8 554 280 726
Fragments arranged by size:
726 554 361 280 8
AvaI C'yCGr_G
Cuts at: 0 16 115 203 1929
Size: 16 99 88 1726
Fragments arranged by size:
1726 99 88 16
BanI G'GyrC_C
Cuts at: 0 79 174 443 1929
Size: 79 95 269 1486
Fragments arranged by size:
1486 269 95 79
BanII G_rGCy'C
Cuts at: 0 210 797 1673 1929
Size: 210 587 876 256
Fragments arranged by size:
876 587 256 210
BbeI G_GCGC'C
Cuts at: 0 178 1929
Size: 178 1751
BbsI GAAGACnn'nnnn_
Cuts at: 0 1084 1691 1929
Size: 1084 607 238
Fragments arranged by size:
1084 607 238
BbvCI CC'TCA_GC
Cuts at: 0 58 1929
Size: 58 1871
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1278 1312 1929
Size: 1278 34 617
Fragments arranged by size:
1278 617 34
BglI GCCn_nnn'nGGC
Cuts at: 0 62 183 1929
Size: 62 121 1746
Fragments arranged by size:
1746 121 62
BlpI GC'TnA_GC
Cuts at: 0 106 296 1929
Size: 106 190 1633
Fragments arranged by size:
1633 190 106
Bme1580I G_kGCm'C
Cuts at: 0 82 210 1929
Size: 82 128 1719
Fragments arranged by size:
1719 128 82
BmgBI CAC'GTC
Cuts at: 0 746 1929
Size: 746 1183
BmrI ACTGGGnnnn_n'
Cuts at: 0 216 1667 1929
Size: 216 1451 262
Fragments arranged by size:
1451 262 216
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 707 1929
Size: 707 1222
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 974 1423 1654 1753 1929
Size: 974 449 231 99 176
Fragments arranged by size:
974 449 231 176 99
Bpu10I CC'TnA_GC
Cuts at: 0 58 164 1929
Size: 58 106 1765
Fragments arranged by size:
1765 106 58
BsaI GGTCTCn'nnnn_
Cuts at: 0 1227 1283 1929
Size: 1227 56 646
Fragments arranged by size:
1227 646 56
BsaAI yAC'GTr
Cuts at: 0 711 949 1929
Size: 711 238 980
Fragments arranged by size:
980 711 238
BsaHI Gr'CG_yC
Cuts at: 0 175 1929
Size: 175 1754
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 958 988 1929
Size: 958 30 941
Fragments arranged by size:
958 941 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1009 1651 1756 1929
Size: 1009 642 105 173
Fragments arranged by size:
1009 642 173 105
BseYI C'CCAG_C
Cuts at: 0 34 75 1364 1929
Size: 34 41 1289 565
Fragments arranged by size:
1289 565 41 34
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 457 1768 1929
Size: 457 1311 161
Fragments arranged by size:
1311 457 161
BsiEI CG_ry'CG
Cuts at: 0 187 552 605 1929
Size: 187 365 53 1324
Fragments arranged by size:
1324 365 187 53
BsiHKAI G_wGCw'C
Cuts at: 0 797 1929
Size: 797 1132
BsmI GAATG_Cn'
Cuts at: 0 641 1929
Size: 641 1288
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1633 1929
Size: 1633 296
Bsp1286I G_dGCh'C
Cuts at: 0 82 210 797 1673 1929
Size: 82 128 587 876 256
Fragments arranged by size:
876 587 256 128 82
BspHI T'CATG_A
Cuts at: 0 906 1929
Size: 906 1023
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 236 1542 1929
Size: 236 1306 387
Fragments arranged by size:
1306 387 236
BsrDI GCAATG_nn'
Cuts at: 0 1382 1929
Size: 1382 547
BsrFI r'CCGG_y
Cuts at: 0 121 171 619 1929
Size: 121 50 448 1310
Fragments arranged by size:
1310 448 121 50
BssHII G'CGCG_C
Cuts at: 0 101 1929
Size: 101 1828
BssSI C'ACGA_G
Cuts at: 0 1697 1929
Size: 1697 232
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1273 1680 1929
Size: 1273 407 249
Fragments arranged by size:
1273 407 249
BtgI C'CryG_G
Cuts at: 0 546 630 863 1895 1929
Size: 546 84 233 1032 34
Fragments arranged by size:
1032 546 233 84 34
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 227 1929
Size: 227 1702
BtsI GCAGTG_nn'
Cuts at: 0 1572 1929
Size: 1572 357
ClaI AT'CG_AT
Cuts at: 0 501 1929
Size: 501 1428
EaeI y'GGCC_r
Cuts at: 0 184 602 866 1929
Size: 184 418 264 1063
Fragments arranged by size:
1063 418 264 184
EagI C'GGCC_G
Cuts at: 0 184 602 1929
Size: 184 418 1327
Fragments arranged by size:
1327 418 184
EarI CTCTTCn'nnn_
Cuts at: 0 16 250 332 422 674 1571 1929
Size: 16 234 82 90 252 897 358
Fragments arranged by size:
897 358 252 234 90 82 16
EcoICRI GAG'CTC
Cuts at: 0 795 1929
Size: 795 1134
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 974 1423 1654 1753 1826 1894 1929
Size: 974 449 231 99 73 68 35
Fragments arranged by size:
974 449 231 99 73 68 35
EcoO109I rG'GnC_Cy
Cuts at: 0 199 726 1434 1929
Size: 199 527 708 495
Fragments arranged by size:
708 527 495 199
EcoRI G'AATT_C
Cuts at: 0 923 1929
Size: 923 1006
FseI GG_CCGG'CC
Cuts at: 0 125 1929
Size: 125 1804
HaeII r_GCGC'y
Cuts at: 0 24 95 178 194 1929
Size: 24 71 83 16 1735
Fragments arranged by size:
1735 83 71 24 16
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 333 335 365 367 1804 1836 1857 1889
Size: 333 2 30 2 1437 32 21 32
Cuts at: 1889 1929
Size: 40
Fragments arranged by size:
1437 333 40 32 32 30 21 2
2
HincII GTy'rAC
Cuts at: 0 1008 1929
Size: 1008 921
KasI G'GCGC_C
Cuts at: 0 174 1929
Size: 174 1755
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 936 1150 1929
Size: 936 214 779
Fragments arranged by size:
936 779 214
MscI TGG'CCA
Cuts at: 0 868 1929
Size: 868 1061
MslI CAynn'nnrTG
Cuts at: 0 535 883 1271 1744 1929
Size: 535 348 388 473 185
Fragments arranged by size:
535 473 388 348 185
MspA1I CmG'CkG
Cuts at: 0 6 62 891 1443 1515 1897 1929
Size: 6 56 829 552 72 382 32
Fragments arranged by size:
829 552 382 72 56 32 6
NaeI GCC'GGC
Cuts at: 0 123 173 1929
Size: 123 50 1756
Fragments arranged by size:
1756 123 50
NarI GG'CG_CC
Cuts at: 0 175 1929
Size: 175 1754
NcoI C'CATG_G
Cuts at: 0 863 1929
Size: 863 1066
NgoMIV G'CCGG_C
Cuts at: 0 121 171 1929
Size: 121 50 1758
Fragments arranged by size:
1758 121 50
NspI r_CATG'y
Cuts at: 0 837 1133 1743 1929
Size: 837 296 610 186
Fragments arranged by size:
837 610 296 186
PciI A'CATG_T
Cuts at: 0 833 1929
Size: 833 1096
PflMI CCAn_nnn'nTGG
Cuts at: 0 423 1031 1929
Size: 423 608 898
Fragments arranged by size:
898 608 423
PpuMI rG'GwC_Cy
Cuts at: 0 199 726 1434 1929
Size: 199 527 708 495
Fragments arranged by size:
708 527 495 199
PshAI GACnn'nnGTC
Cuts at: 0 766 1622 1929
Size: 766 856 307
Fragments arranged by size:
856 766 307
PspOMI G'GGCC_C
Cuts at: 0 206 1929
Size: 206 1723
PspXI vC'TCGA_Gb
Cuts at: 0 16 1929
Size: 16 1913
PstI C_TGCA'G
Cuts at: 0 161 663 1433 1929
Size: 161 502 770 496
Fragments arranged by size:
770 502 496 161
PvuII CAG'CTG
Cuts at: 0 891 1443 1929
Size: 891 552 486
Fragments arranged by size:
891 552 486
SacI G_AGCT'C
Cuts at: 0 797 1929
Size: 797 1132
SacII CC_GC'GG
Cuts at: 0 1898 1929
Size: 1898 31
SanDI GG'GwC_CC
Cuts at: 0 199 1929
Size: 199 1730
SapI GCTCTTCn'nnn_
Cuts at: 0 16 1929
Size: 16 1913
SfcI C'TryA_G
Cuts at: 0 157 659 1214 1429 1929
Size: 157 502 555 215 500
Fragments arranged by size:
555 502 500 215 157
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 62 1929
Size: 62 1867
SfoI GGC'GCC
Cuts at: 0 176 1929
Size: 176 1753
SgrAI Cr'CCGG_yG
Cuts at: 0 171 1929
Size: 171 1758
SmaI CCC'GGG
Cuts at: 0 205 1929
Size: 205 1724
SmlI C'TyrA_G
Cuts at: 0 16 1929
Size: 16 1913
SnaBI TAC'GTA
Cuts at: 0 711 1929
Size: 711 1218
SphI G_CATG'C
Cuts at: 0 1743 1929
Size: 1743 186
SspI AAT'ATT
Cuts at: 0 1149 1476 1929
Size: 1149 327 453
Fragments arranged by size:
1149 453 327
StyI C'CwwG_G
Cuts at: 0 644 863 1929
Size: 644 219 1066
Fragments arranged by size:
1066 644 219
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 643 1615 1929
Size: 643 972 314
Fragments arranged by size:
972 643 314
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1107 1139 1929
Size: 1107 32 790
Fragments arranged by size:
1107 790 32
Tth111I GACn'n_nGTC
Cuts at: 0 784 1929
Size: 784 1145
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 870 1929
Size: 870 1059
XhoI C'TCGA_G
Cuts at: 0 16 1929
Size: 16 1913
XmaI C'CCGG_G
Cuts at: 0 203 1929
Size: 203 1726
Enzymes that do cut:
AclI AcuI AfeI AflIII AhdI AlfI AlwNI ApaI
ApoI AvaI BanI BanII BbeI BbsI BbvCI BcgI
BglI BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BsaI BsaAI BsaHI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspHI BspMI BsrDI BsrFI
BssHII BssSI BstXI BtgI BtgZI BtsI ClaI EaeI
EagI EarI EcoICRI Eco57MI EcoO109I EcoRI FseI HaeII
Hin4I HincII KasI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PciI PflMI PpuMI PshAI
PspOMI PspXI PstI PvuII SacI SacII SanDI SapI
SfcI SfiI SfoI SgrAI SmaI SmlI SnaBI SphI
SspI StyI TaqII TstI Tth111I XcmI XhoI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AflII AgeI AleI AloI
ApaLI AscI AseI AsiSI AvrII BaeI BamHI BciVI
BclI BfrBI BglII BmtI BpuEI BsaBI BsaWI BsiWI
BspEI BsrBI BsrGI BstAPI BstBI BstEII BstYI BstZ17I
Bsu36I CspCI DraI DraIII DrdI EciI EcoNI EcoRV
FalI FspI FspAI HindIII HpaI KpnI MfeI MluI
NdeI NheI NotI NruI NsiI PacI PasI PfoI
PmeI PmlI PpiI PsiI PsrI PvuI RsrII SalI
SbfI ScaI SexAI SgfI SpeI SrfI StuI SwaI
TatI XbaI XmnI ZraI