Restriction map for
pFN21AE3263
(Linear) (Six-base) MAPSORT of: KIEE3263.seq Check: 2259 from: 1 to: 1902
KIEE3263.orf
With 182 enzymes: SgfI *
March 22, 2012 16:28 ..
AccI GT'mk_AC
Cuts at: 0 659 1902
Size: 659 1243
Acc65I G'GTAC_C
Cuts at: 0 524 1902
Size: 524 1378
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 406 920 956 1154 1222 1902
Size: 406 514 36 198 68 680
Fragments arranged by size:
680 514 406 198 68 36
AflIII A'CryG_T
Cuts at: 0 1833 1835 1902
Size: 1833 2 67
Fragments arranged by size:
1833 67 2
AleI CACnn'nnGTG
Cuts at: 0 740 1902
Size: 740 1162
AlwNI CAG_nnn'CTG
Cuts at: 0 1387 1902
Size: 1387 515
ApaLI G'TGCA_C
Cuts at: 0 411 1902
Size: 411 1491
ApoI r'AATT_y
Cuts at: 0 154 191 309 1260 1457 1481 1902
Size: 154 37 118 951 197 24 421
Fragments arranged by size:
951 421 197 154 118 37 24
BamHI G'GATC_C
Cuts at: 0 671 1902
Size: 671 1231
BanI G'GyrC_C
Cuts at: 0 33 524 1902
Size: 33 491 1378
Fragments arranged by size:
1378 491 33
BbsI GAAGACnn'nnnn_
Cuts at: 0 79 1424 1673 1902
Size: 79 1345 249 229
Fragments arranged by size:
1345 249 229 79
BbvCI CC'TCA_GC
Cuts at: 0 793 1902
Size: 793 1109
BclI T'GATC_A
Cuts at: 0 419 906 1902
Size: 419 487 996
Fragments arranged by size:
996 487 419
BglII A'GATC_T
Cuts at: 0 1197 1902
Size: 1197 705
Bme1580I G_kGCm'C
Cuts at: 0 415 626 1902
Size: 415 211 1276
Fragments arranged by size:
1276 415 211
BmgBI CAC'GTC
Cuts at: 0 576 1902
Size: 576 1326
BmrI ACTGGGnnnn_n'
Cuts at: 0 1257 1902
Size: 1257 645
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1727 1902
Size: 1727 175
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 709 1409 1507 1902
Size: 709 700 98 395
Fragments arranged by size:
709 700 395 98
Bpu10I CC'TnA_GC
Cuts at: 0 793 1902
Size: 793 1109
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1222 1902
Size: 1222 680
BsaI GGTCTCn'nnnn_
Cuts at: 0 949 1902
Size: 949 953
BsaAI yAC'GTr
Cuts at: 0 1723 1836 1902
Size: 1723 113 66
Fragments arranged by size:
1723 113 66
BsaBI GATnn'nnATC
Cuts at: 0 224 1902
Size: 224 1678
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1689 1719 1902
Size: 1689 30 183
Fragments arranged by size:
1689 183 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1186 1902
Size: 1186 716
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1063 1902
Size: 1063 839
BsiHKAI G_wGCw'C
Cuts at: 0 415 1902
Size: 415 1487
BsmI GAATG_Cn'
Cuts at: 0 1788 1902
Size: 1788 114
Bsp1286I G_dGCh'C
Cuts at: 0 415 626 1902
Size: 415 211 1276
Fragments arranged by size:
1276 415 211
BspHI T'CATG_A
Cuts at: 0 1337 1902
Size: 1337 565
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 642 1902
Size: 642 1260
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1295 1902
Size: 1295 607
BstYI r'GATC_y
Cuts at: 0 671 1197 1233 1633 1902
Size: 671 526 36 400 269
Fragments arranged by size:
671 526 400 269 36
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 106 1902
Size: 106 1796
BtsI GCAGTG_nn'
Cuts at: 0 722 784 1902
Size: 722 62 1118
Fragments arranged by size:
1118 722 62
DrdI GACnn_nn'nnGTC
Cuts at: 0 580 1902
Size: 580 1322
EarI CTCTTCn'nnn_
Cuts at: 0 817 838 874 1150 1180 1364 1418 1867
Size: 817 21 36 276 30 184 54 449
Cuts at: 1867 1902
Size: 35
Fragments arranged by size:
817 449 276 184 54 36 35 30
21
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 30 1840 1902
Size: 30 1810 62
Fragments arranged by size:
1810 62 30
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 406 709 920 956 1154 1222 1409 1507
Size: 406 303 211 36 198 68 187 98
Cuts at: 1507 1902
Size: 395
Fragments arranged by size:
406 395 303 211 198 187 98 68
36
EcoNI CCTnn'n_nnAGG
Cuts at: 0 970 1902
Size: 970 932
EcoO109I rG'GnC_Cy
Cuts at: 0 506 1118 1513 1902
Size: 506 612 395 389
Fragments arranged by size:
612 506 395 389
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 564 1902
Size: 564 1338
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 916 948 1390 1422 1560 1592 1902
Size: 916 32 442 32 138 32 310
Fragments arranged by size:
916 442 310 138 32 32 32
HincII GTy'rAC
Cuts at: 0 1587 1902
Size: 1587 315
KpnI G_GTAC'C
Cuts at: 0 528 1902
Size: 528 1374
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 115 1464 1902
Size: 115 1349 438
Fragments arranged by size:
1349 438 115
MslI CAynn'nnrTG
Cuts at: 0 709 740 1902
Size: 709 31 1162
Fragments arranged by size:
1162 709 31
MspA1I CmG'CkG
Cuts at: 0 374 384 1384 1446 1902
Size: 374 10 1000 62 456
Fragments arranged by size:
1000 456 374 62 10
PasI CC'CwG_GG
Cuts at: 0 399 1902
Size: 399 1503
PflMI CCAn_nnn'nTGG
Cuts at: 0 535 687 1902
Size: 535 152 1215
Fragments arranged by size:
1215 535 152
PfoI T'CCnGG_A
Cuts at: 0 674 1177 1636 1902
Size: 674 503 459 266
Fragments arranged by size:
674 503 459 266
PmlI CAC'GTG
Cuts at: 0 1723 1836 1902
Size: 1723 113 66
Fragments arranged by size:
1723 113 66
PpuMI rG'GwC_Cy
Cuts at: 0 506 1513 1902
Size: 506 1007 389
Fragments arranged by size:
1007 506 389
PsiI TTA'TAA
Cuts at: 0 635 1902
Size: 635 1267
PvuII CAG'CTG
Cuts at: 0 384 1384 1446 1902
Size: 384 1000 62 456
Fragments arranged by size:
1000 456 384 62
SanDI GG'GwC_CC
Cuts at: 0 1513 1902
Size: 1513 389
SapI GCTCTTCn'nnn_
Cuts at: 0 1364 1902
Size: 1364 538
ScaI AGT'ACT
Cuts at: 0 1246 1902
Size: 1246 656
SmlI C'TyrA_G
Cuts at: 0 1201 1902
Size: 1201 701
SspI AAT'ATT
Cuts at: 0 252 300 917 1902
Size: 252 48 617 985
Fragments arranged by size:
985 617 252 48
StyI C'CwwG_G
Cuts at: 0 44 1902
Size: 44 1858
TatI w'GTAC_w
Cuts at: 0 1244 1902
Size: 1244 658
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1040 1072 1902
Size: 1040 32 830
Fragments arranged by size:
1040 830 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 312 726 1902
Size: 312 414 1176
Fragments arranged by size:
1176 414 312
Enzymes that do cut:
AccI Acc65I AcuI AflIII AleI AlwNI ApaLI ApoI
BamHI BanI BbsI BbvCI BclI BglII Bme1580I BmgBI
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaXI BseRI BsgI BsiHKAI BsmI Bsp1286I BspHI BspMI
BstXI BstYI BtgZI BtsI DrdI EarI EciI Eco57MI
EcoNI EcoO109I FalI Hin4I HincII KpnI MmeI MslI
MspA1I PasI PflMI PfoI PmlI PpuMI PsiI PvuII
SanDI SapI ScaI SmlI SspI StyI TatI TstI
XcmI
Enzymes that do not cut:
AarI AatII AclI AfeI AflII AgeI AhdI AlfI
AloI ApaI AscI AseI AsiSI AvaI AvrII BaeI
BanII BbeI BcgI BciVI BfrBI BglI BlpI BmtI
BsaHI BsaWI BseYI BsiEI BsiWI BsmBI BspEI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstZ17I Bsu36I BtgI ClaI CspCI DraI DraIII EaeI
EagI EcoICRI EcoRI EcoRV FseI FspI FspAI HaeII
HindIII HpaI KasI MfeI MluI MscI NaeI NarI
NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI
PacI PciI PmeI PpiI PshAI PspOMI PspXI PsrI
PstI PvuI RsrII SacI SacII SalI SbfI SexAI
SfcI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI
SphI SrfI StuI SwaI TaqII Tth111I XbaI XhoI
XmaI XmnI ZraI