Restriction map for
pFN21AE3258
(Linear) (Six-base) MAPSORT of: KIEE3258.seq Check: 9080 from: 1 to: 1851
KIEE3258.orf
With 182 enzymes: SgfI *
July 3, 2012 17:32 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 26 91 95 247 1619 1851
Size: 26 65 4 152 1372 232
Fragments arranged by size:
1372 232 152 65 26 4
AflII C'TTAA_G
Cuts at: 0 481 1851
Size: 481 1370
AflIII A'CryG_T
Cuts at: 0 1455 1851
Size: 1455 396
AleI CACnn'nnGTG
Cuts at: 0 462 1851
Size: 462 1389
AlwNI CAG_nnn'CTG
Cuts at: 0 1189 1851
Size: 1189 662
ApaI G_GGCC'C
Cuts at: 0 1718 1851
Size: 1718 133
ApoI r'AATT_y
Cuts at: 0 411 516 1068 1851
Size: 411 105 552 783
Fragments arranged by size:
783 552 411 105
AseI AT'TA_AT
Cuts at: 0 1556 1851
Size: 1556 295
AvaI C'yCGr_G
Cuts at: 0 298 1851
Size: 298 1553
BanII G_rGCy'C
Cuts at: 0 1292 1718 1851
Size: 1292 426 133
Fragments arranged by size:
1292 426 133
BbsI GAAGACnn'nnnn_
Cuts at: 0 329 594 749 874 1094 1851
Size: 329 265 155 125 220 757
Fragments arranged by size:
757 329 265 220 155 125
BclI T'GATC_A
Cuts at: 0 164 1851
Size: 164 1687
BglII A'GATC_T
Cuts at: 0 1414 1761 1851
Size: 1414 347 90
Fragments arranged by size:
1414 347 90
Bme1580I G_kGCm'C
Cuts at: 0 1718 1851
Size: 1718 133
BmrI ACTGGGnnnn_n'
Cuts at: 0 146 748 1121 1761 1851
Size: 146 602 373 640 90
Fragments arranged by size:
640 602 373 146 90
Bpu10I CC'TnA_GC
Cuts at: 0 852 1851
Size: 852 999
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1047 1851
Size: 1047 804
BsaBI GATnn'nnATC
Cuts at: 0 233 1057 1419 1760 1851
Size: 233 824 362 341 91
Fragments arranged by size:
824 362 341 233 91
BsaWI w'CCGG_w
Cuts at: 0 1140 1851
Size: 1140 711
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 563 676 775 1851
Size: 563 113 99 1076
Fragments arranged by size:
1076 563 113 99
BseYI C'CCAG_C
Cuts at: 0 612 1264 1851
Size: 612 652 587
Fragments arranged by size:
652 612 587
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 365 1352 1851
Size: 365 987 499
Fragments arranged by size:
987 499 365
Bsp1286I G_dGCh'C
Cuts at: 0 1292 1718 1851
Size: 1292 426 133
Fragments arranged by size:
1292 426 133
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1616 1851
Size: 1616 235
BsrBI CCG'CTC
Cuts at: 0 1146 1851
Size: 1146 705
BsrDI GCAATG_nn'
Cuts at: 0 713 1844 1851
Size: 713 1131 7
Fragments arranged by size:
1131 713 7
BstEII G'GTnAC_C
Cuts at: 0 1603 1851
Size: 1603 248
BstXI CCAn_nnnn'nTGG
Cuts at: 0 25 1851
Size: 25 1826
BstYI r'GATC_y
Cuts at: 0 1414 1761 1851
Size: 1414 347 90
Fragments arranged by size:
1414 347 90
Bsu36I CC'TnA_GG
Cuts at: 0 124 1851
Size: 124 1727
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 296 1851
Size: 296 1555
BtsI GCAGTG_nn'
Cuts at: 0 469 1851
Size: 469 1382
DraI TTT'AAA
Cuts at: 0 537 1310 1851
Size: 537 773 541
Fragments arranged by size:
773 541 537
DraIII CAC_nnn'GTG
Cuts at: 0 316 1258 1851
Size: 316 942 593
Fragments arranged by size:
942 593 316
EaeI y'GGCC_r
Cuts at: 0 466 1523 1851
Size: 466 1057 328
Fragments arranged by size:
1057 466 328
EarI CTCTTCn'nnn_
Cuts at: 0 506 782 1610 1851
Size: 506 276 828 241
Fragments arranged by size:
828 506 276 241
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 26 91 95 247 1619 1851
Size: 26 65 4 152 1372 232
Fragments arranged by size:
1372 232 152 65 26 4
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1586 1851
Size: 1586 265
EcoO109I rG'GnC_Cy
Cuts at: 0 1275 1714 1715 1851
Size: 1275 439 1 136
Fragments arranged by size:
1275 439 136 1
EcoRI G'AATT_C
Cuts at: 0 411 516 1851
Size: 411 105 1335
Fragments arranged by size:
1335 411 105
EcoRV GAT'ATC
Cuts at: 0 696 1851
Size: 696 1155
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 898 1415 1851
Size: 898 517 436
Fragments arranged by size:
898 517 436
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 361 393 892 904 924 936 1021 1053
Size: 361 32 499 12 20 12 85 32
Cuts at: 1053 1165 1197 1851
Size: 112 32 654
Fragments arranged by size:
654 499 361 112 85 32 32 32
20 12 12
HincII GTy'rAC
Cuts at: 0 1167 1851
Size: 1167 684
HindIII A'AGCT_T
Cuts at: 0 347 478 1851
Size: 347 131 1373
Fragments arranged by size:
1373 347 131
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 110 219 1851
Size: 110 109 1632
Fragments arranged by size:
1632 110 109
MscI TGG'CCA
Cuts at: 0 468 1525 1851
Size: 468 1057 326
Fragments arranged by size:
1057 468 326
MslI CAynn'nnrTG
Cuts at: 0 462 1851
Size: 462 1389
MspA1I CmG'CkG
Cuts at: 0 102 1050 1851
Size: 102 948 801
Fragments arranged by size:
948 801 102
NdeI CA'TA_TG
Cuts at: 0 1479 1851
Size: 1479 372
NspI r_CATG'y
Cuts at: 0 492 1459 1851
Size: 492 967 392
Fragments arranged by size:
967 492 392
PciI A'CATG_T
Cuts at: 0 1455 1851
Size: 1455 396
PflMI CCAn_nnn'nTGG
Cuts at: 0 612 1851
Size: 612 1239
PfoI T'CCnGG_A
Cuts at: 0 869 1851
Size: 869 982
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 107 139 1851
Size: 107 32 1712
Fragments arranged by size:
1712 107 32
PsiI TTA'TAA
Cuts at: 0 233 1851
Size: 233 1618
PspOMI G'GGCC_C
Cuts at: 0 1714 1851
Size: 1714 137
PstI C_TGCA'G
Cuts at: 0 963 1613 1851
Size: 963 650 238
Fragments arranged by size:
963 650 238
PvuII CAG'CTG
Cuts at: 0 102 1050 1851
Size: 102 948 801
Fragments arranged by size:
948 801 102
SbfI CC_TGCA'GG
Cuts at: 0 1613 1851
Size: 1613 238
ScaI AGT'ACT
Cuts at: 0 1519 1750 1851
Size: 1519 231 101
Fragments arranged by size:
1519 231 101
SexAI A'CCwGG_T
Cuts at: 0 1254 1851
Size: 1254 597
SfcI C'TryA_G
Cuts at: 0 959 1609 1851
Size: 959 650 242
Fragments arranged by size:
959 650 242
SmlI C'TyrA_G
Cuts at: 0 481 1062 1851
Size: 481 581 789
Fragments arranged by size:
789 581 481
StyI C'CwwG_G
Cuts at: 0 243 1390 1851
Size: 243 1147 461
Fragments arranged by size:
1147 461 243
TatI w'GTAC_w
Cuts at: 0 179 1517 1748 1851
Size: 179 1338 231 103
Fragments arranged by size:
1338 231 179 103
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1314 1346 1851
Size: 1314 32 505
Fragments arranged by size:
1314 505 32
XbaI T'CTAG_A
Cuts at: 0 1417 1851
Size: 1417 434
XmnI GAAnn'nnTTC
Cuts at: 0 881 1851
Size: 881 970
Enzymes that do cut:
AcuI AflII AflIII AleI AlwNI ApaI ApoI AseI
AvaI BanII BbsI BclI BglII Bme1580I BmrI Bpu10I
BpuEI BsaBI BsaWI BseRI BseYI BsgI Bsp1286I BspMI
BsrBI BsrDI BstEII BstXI BstYI Bsu36I BtgZI BtsI
DraI DraIII EaeI EarI Eco57MI EcoNI EcoO109I EcoRI
EcoRV FalI Hin4I HincII HindIII MmeI MscI MslI
MspA1I NdeI NspI PciI PflMI PfoI PpiI PsiI
PspOMI PstI PvuII SbfI ScaI SexAI SfcI SmlI
StyI TatI TstI XbaI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AgeI AhdI
AlfI AloI ApaLI AscI AsiSI AvrII BaeI BamHI
BanI BbeI BbvCI BcgI BciVI BfrBI BglI BlpI
BmgBI BmtI BplI BpmI BsaI BsaAI BsaHI BsaXI
BsiEI BsiHKAI BsiWI BsmI BsmBI BspEI BspHI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstZ17I BtgI ClaI
CspCI DrdI EagI EciI EcoICRI FseI FspI FspAI
HaeII HpaI KasI KpnI MfeI MluI NaeI NarI
NcoI NgoMIV NheI NotI NruI NsiI PacI PasI
PmeI PmlI PpuMI PshAI PspXI PsrI PvuI RsrII
SacI SacII SalI SanDI SapI SfiI SfoI SgfI
SgrAI SmaI SnaBI SpeI SphI SrfI SspI StuI
SwaI TaqII Tth111I XcmI XhoI XmaI ZraI