Restriction map for
pFN21AE3253
(Linear) (Six-base) MAPSORT of: KIEE3253.seq Check: 6618 from: 1 to: 1767
KIEE3253.orf
With 182 enzymes: SgfI *
July 3, 2012 17:31 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 556 1767
Size: 556 1211
AatII G_ACGT'C
Cuts at: 0 621 1767
Size: 621 1146
Acc65I G'GTAC_C
Cuts at: 0 480 1767
Size: 480 1287
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 238 623 1767
Size: 238 385 1144
Fragments arranged by size:
1144 385 238
AfeI AGC'GCT
Cuts at: 0 427 1767
Size: 427 1340
AleI CACnn'nnGTG
Cuts at: 0 304 1444 1767
Size: 304 1140 323
Fragments arranged by size:
1140 323 304
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 416 1462 1767
Size: 416 1046 305
Fragments arranged by size:
1046 416 305
AlwNI CAG_nnn'CTG
Cuts at: 0 559 687 1530 1767
Size: 559 128 843 237
Fragments arranged by size:
843 559 237 128
ApaLI G'TGCA_C
Cuts at: 0 401 1767
Size: 401 1366
ApoI r'AATT_y
Cuts at: 0 22 847 1767
Size: 22 825 920
Fragments arranged by size:
920 825 22
AvaI C'yCGr_G
Cuts at: 0 698 1767
Size: 698 1069
BanI G'GyrC_C
Cuts at: 0 80 480 1438 1599 1767
Size: 80 400 958 161 168
Fragments arranged by size:
958 400 168 161 80
BanII G_rGCy'C
Cuts at: 0 228 627 1767
Size: 228 399 1140
Fragments arranged by size:
1140 399 228
BbsI GAAGACnn'nnnn_
Cuts at: 0 264 1490 1767
Size: 264 1226 277
Fragments arranged by size:
1226 277 264
BbvCI CC'TCA_GC
Cuts at: 0 631 1258 1767
Size: 631 627 509
Fragments arranged by size:
631 627 509
BglI GCCn_nnn'nGGC
Cuts at: 0 320 764 1133 1436 1767
Size: 320 444 369 303 331
Fragments arranged by size:
444 369 331 320 303
Bme1580I G_kGCm'C
Cuts at: 0 311 405 1093 1767
Size: 311 94 688 674
Fragments arranged by size:
688 674 311 94
BmrI ACTGGGnnnn_n'
Cuts at: 0 14 1767
Size: 14 1753
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 63 1767
Size: 63 1704
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 955 1507 1678 1767
Size: 955 552 171 89
Fragments arranged by size:
955 552 171 89
Bpu10I CC'TnA_GC
Cuts at: 0 631 1258 1767
Size: 631 627 509
Fragments arranged by size:
631 627 509
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1203 1767
Size: 1203 564
BsaI GGTCTCn'nnnn_
Cuts at: 0 60 736 1767
Size: 60 676 1031
Fragments arranged by size:
1031 676 60
BsaHI Gr'CG_yC
Cuts at: 0 530 618 1767
Size: 530 88 1149
Fragments arranged by size:
1149 530 88
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 39 48 51 54 57 151 451 556
Size: 39 9 3 3 3 94 300 105
Cuts at: 556 679 988 1228 1231 1240 1767
Size: 123 309 240 3 9 527
Fragments arranged by size:
527 309 300 240 123 105 94 39
9 9 3 3 3 3
BseYI C'CCAG_C
Cuts at: 0 310 475 606 961 1026 1453 1737 1767
Size: 310 165 131 355 65 427 284 30
Fragments arranged by size:
427 355 310 284 165 131 65 30
BsiEI CG_ry'CG
Cuts at: 0 660 1767
Size: 660 1107
BsiHKAI G_wGCw'C
Cuts at: 0 405 627 1767
Size: 405 222 1140
Fragments arranged by size:
1140 405 222
BsmI GAATG_Cn'
Cuts at: 0 793 1374 1767
Size: 793 581 393
Fragments arranged by size:
793 581 393
Bsp1286I G_dGCh'C
Cuts at: 0 228 311 405 627 1093 1767
Size: 228 83 94 222 466 674
Fragments arranged by size:
674 466 228 222 94 83
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 556 754 1767
Size: 556 198 1013
Fragments arranged by size:
1013 556 198
BsrFI r'CCGG_y
Cuts at: 0 572 1761 1767
Size: 572 1189 6
Fragments arranged by size:
1189 572 6
BssSI C'ACGA_G
Cuts at: 0 409 1767
Size: 409 1358
BstAPI GCAn_nnn'nTGC
Cuts at: 0 687 773 1767
Size: 687 86 994
Fragments arranged by size:
994 687 86
BstYI r'GATC_y
Cuts at: 0 728 1485 1701 1767
Size: 728 757 216 66
Fragments arranged by size:
757 728 216 66
BtgI C'CryG_G
Cuts at: 0 315 833 1767
Size: 315 518 934
Fragments arranged by size:
934 518 315
BtsI GCAGTG_nn'
Cuts at: 0 463 685 717 1767
Size: 463 222 32 1050
Fragments arranged by size:
1050 463 222 32
ClaI AT'CG_AT
Cuts at: 0 656 1767
Size: 656 1111
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 337 372 1767
Size: 337 35 1395
Fragments arranged by size:
1395 337 35
DrdI GACnn_nn'nnGTC
Cuts at: 0 553 1767
Size: 553 1214
EaeI y'GGCC_r
Cuts at: 0 570 1001 1310 1467 1767
Size: 570 431 309 157 300
Fragments arranged by size:
570 431 309 300 157
EarI CTCTTCn'nnn_
Cuts at: 0 10 75 114 1217 1767
Size: 10 65 39 1103 550
Fragments arranged by size:
1103 550 65 39 10
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 409 1767
Size: 409 1358
EcoICRI GAG'CTC
Cuts at: 0 625 1767
Size: 625 1142
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 238 623 955 1507 1678 1767
Size: 238 385 332 552 171 89
Fragments arranged by size:
552 385 332 238 171 89
EcoO109I rG'GnC_Cy
Cuts at: 0 145 939 978 1549 1767
Size: 145 794 39 571 218
Fragments arranged by size:
794 571 218 145 39
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1447 1767
Size: 1447 320
FspI TGC'GCA
Cuts at: 0 386 1767
Size: 386 1381
HaeII r_GCGC'y
Cuts at: 0 240 429 1767
Size: 240 189 1338
Fragments arranged by size:
1338 240 189
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 34 66 517 549 1767
Size: 34 32 451 32 1218
Fragments arranged by size:
1218 451 34 32 32
HindIII A'AGCT_T
Cuts at: 0 463 1767
Size: 463 1304
KpnI G_GTAC'C
Cuts at: 0 484 1767
Size: 484 1283
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 425 557 884 1767
Size: 425 132 327 883
Fragments arranged by size:
883 425 327 132
MscI TGG'CCA
Cuts at: 0 1003 1312 1767
Size: 1003 309 455
Fragments arranged by size:
1003 455 309
MslI CAynn'nnrTG
Cuts at: 0 304 1444 1767
Size: 304 1140 323
Fragments arranged by size:
1140 323 304
MspA1I CmG'CkG
Cuts at: 0 33 287 475 752 1040 1262 1276 1316
Size: 33 254 188 277 288 222 14 40
Cuts at: 1316 1388 1767
Size: 72 379
Fragments arranged by size:
379 288 277 254 222 188 72 40
33 14
NcoI C'CATG_G
Cuts at: 0 833 1767
Size: 833 934
PasI CC'CwG_GG
Cuts at: 0 149 948 1767
Size: 149 799 819
Fragments arranged by size:
819 799 149
PfoI T'CCnGG_A
Cuts at: 0 1358 1655 1767
Size: 1358 297 112
Fragments arranged by size:
1358 297 112
PstI C_TGCA'G
Cuts at: 0 458 683 751 757 814 902 1045 1767
Size: 458 225 68 6 57 88 143 722
Fragments arranged by size:
722 458 225 143 88 68 57 6
PvuI CG_AT'CG
Cuts at: 0 660 1767
Size: 660 1107
PvuII CAG'CTG
Cuts at: 0 33 475 752 1040 1262 1276 1316 1388
Size: 33 442 277 288 222 14 40 72
Cuts at: 1388 1767
Size: 379
Fragments arranged by size:
442 379 288 277 222 72 40 33
14
SacI G_AGCT'C
Cuts at: 0 627 1767
Size: 627 1140
SapI GCTCTTCn'nnn_
Cuts at: 0 114 1767
Size: 114 1653
SfcI C'TryA_G
Cuts at: 0 454 595 679 747 753 810 898 1041
Size: 454 141 84 68 6 57 88 143
Cuts at: 1041 1338 1767
Size: 297 429
Fragments arranged by size:
454 429 297 143 141 88 84 68
57 6
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1133 1767
Size: 1133 634
SmaI CCC'GGG
Cuts at: 0 700 1767
Size: 700 1067
SmlI C'TyrA_G
Cuts at: 0 1182 1767
Size: 1182 585
SpeI A'CTAG_T
Cuts at: 0 1093 1291 1767
Size: 1093 198 476
Fragments arranged by size:
1093 476 198
StuI AGG'CCT
Cuts at: 0 1127 1767
Size: 1127 640
StyI C'CwwG_G
Cuts at: 0 833 1767
Size: 833 934
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 42 68 975 1132 1136 1467 1767
Size: 42 26 907 157 4 331 300
Fragments arranged by size:
907 331 300 157 42 26 4
TatI w'GTAC_w
Cuts at: 0 325 1767
Size: 325 1442
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1592 1624 1767
Size: 1592 32 143
Fragments arranged by size:
1592 143 32
Tth111I GACn'n_nGTC
Cuts at: 0 1732 1767
Size: 1732 35
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 840 1330 1767
Size: 840 490 437
Fragments arranged by size:
840 490 437
XmaI C'CCGG_G
Cuts at: 0 698 1767
Size: 698 1069
ZraI GAC'GTC
Cuts at: 0 619 1767
Size: 619 1148
Enzymes that do cut:
AarI AatII Acc65I AcuI AfeI AleI AlfI AlwNI
ApaLI ApoI AvaI BanI BanII BbsI BbvCI BglI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaHI
BseRI BseYI BsiEI BsiHKAI BsmI Bsp1286I BspMI BsrFI
BssSI BstAPI BstYI BtgI BtsI ClaI CspCI DrdI
EaeI EarI EciI EcoICRI Eco57MI EcoO109I FalI FspI
HaeII Hin4I HindIII KpnI MmeI MscI MslI MspA1I
NcoI PasI PfoI PstI PvuI PvuII SacI SapI
SfcI SfiI SmaI SmlI SpeI StuI StyI TaqII
TatI TstI Tth111I XcmI XmaI ZraI
Enzymes that do not cut:
AccI AclI AflII AflIII AgeI AhdI AloI ApaI
AscI AseI AsiSI AvrII BaeI BamHI BbeI BcgI
BciVI BclI BfrBI BglII BlpI BmgBI BmtI BsaAI
BsaBI BsaWI BsaXI BsgI BsiWI BsmBI BspEI BspHI
BsrBI BsrDI BsrGI BssHII BstBI BstEII BstXI BstZ17I
Bsu36I BtgZI DraI DraIII EagI EcoNI EcoRI EcoRV
FseI FspAI HincII HpaI KasI MfeI MluI NaeI
NarI NdeI NgoMIV NheI NotI NruI NsiI NspI
PacI PciI PflMI PmeI PmlI PpiI PpuMI PshAI
PsiI PspOMI PspXI PsrI RsrII SacII SalI SanDI
SbfI ScaI SexAI SfoI SgfI SgrAI SnaBI SphI
SrfI SspI SwaI XbaI XhoI XmnI