Restriction map for
pFN21AE3250
(Linear) (Six-base) MAPSORT of: KIEE3250.seq Check: 7624 from: 1 to: 1731
KIEE3250.orf
With 182 enzymes: SgfI *
March 22, 2012 16:27 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1341 1731
Size: 1341 390
AflII C'TTAA_G
Cuts at: 0 1441 1731
Size: 1441 290
AflIII A'CryG_T
Cuts at: 0 454 1731
Size: 454 1277
AleI CACnn'nnGTG
Cuts at: 0 455 1731
Size: 455 1276
ApoI r'AATT_y
Cuts at: 0 302 913 1731
Size: 302 611 818
Fragments arranged by size:
818 611 302
AvaI C'yCGr_G
Cuts at: 0 4 15 1731
Size: 4 11 1716
Fragments arranged by size:
1716 11 4
BanI G'GyrC_C
Cuts at: 0 519 1731
Size: 519 1212
Bme1580I G_kGCm'C
Cuts at: 0 985 1731
Size: 985 746
BmrI ACTGGGnnnn_n'
Cuts at: 0 738 1731
Size: 738 993
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 247 329 915 983 1067 1151 1235 1251
Size: 247 82 586 68 84 84 84 16
Cuts at: 1251 1319 1335 1487 1571 1731
Size: 68 16 152 84 160
Fragments arranged by size:
586 247 160 152 84 84 84 84
82 68 68 16 16
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 414 1731
Size: 414 1317
BsaHI Gr'CG_yC
Cuts at: 0 650 1731
Size: 650 1081
BsaWI w'CCGG_w
Cuts at: 0 1283 1731
Size: 1283 448
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 302 332 1731
Size: 302 30 1399
Fragments arranged by size:
1399 302 30
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 834 1338 1506 1590 1674 1731
Size: 834 504 168 84 84 57
Fragments arranged by size:
834 504 168 84 84 57
BsiEI CG_ry'CG
Cuts at: 0 68 1731
Size: 68 1663
BsmI GAATG_Cn'
Cuts at: 0 366 901 1153 1731
Size: 366 535 252 578
Fragments arranged by size:
578 535 366 252
Bsp1286I G_dGCh'C
Cuts at: 0 985 1731
Size: 985 746
BspHI T'CATG_A
Cuts at: 0 1227 1731
Size: 1227 504
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 604 1731
Size: 604 1127
BsrDI GCAATG_nn'
Cuts at: 0 906 1242 1731
Size: 906 336 489
Fragments arranged by size:
906 489 336
BstYI r'GATC_y
Cuts at: 0 180 1731
Size: 180 1551
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1099 1731
Size: 1099 632
BtsI GCAGTG_nn'
Cuts at: 0 542 1494 1578 1731
Size: 542 952 84 153
Fragments arranged by size:
952 542 153 84
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 888 923 1224 1259 1731
Size: 888 35 301 35 472
Fragments arranged by size:
888 472 301 35 35
EaeI y'GGCC_r
Cuts at: 0 152 623 1731
Size: 152 471 1108
Fragments arranged by size:
1108 471 152
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 34 1731
Size: 34 1697
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 247 329 915 983 1067 1151 1235 1251
Size: 247 82 586 68 84 84 84 16
Cuts at: 1251 1319 1335 1341 1487 1571 1731
Size: 68 16 6 146 84 160
Fragments arranged by size:
586 247 160 146 84 84 84 84
82 68 68 16 16 6
EcoNI CCTnn'n_nnAGG
Cuts at: 0 190 1731
Size: 190 1541
EcoO109I rG'GnC_Cy
Cuts at: 0 78 1731
Size: 78 1653
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 532 564 836 868 1731
Size: 532 32 272 32 863
Fragments arranged by size:
863 532 272 32 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 560 1124 1460 1731
Size: 560 564 336 271
Fragments arranged by size:
564 560 336 271
MscI TGG'CCA
Cuts at: 0 625 1731
Size: 625 1106
MslI CAynn'nnrTG
Cuts at: 0 455 1731
Size: 455 1276
NspI r_CATG'y
Cuts at: 0 458 836 1731
Size: 458 378 895
Fragments arranged by size:
895 458 378
PasI CC'CwG_GG
Cuts at: 0 252 1731
Size: 252 1479
PciI A'CATG_T
Cuts at: 0 454 1731
Size: 454 1277
PpuMI rG'GwC_Cy
Cuts at: 0 78 1731
Size: 78 1653
PstI C_TGCA'G
Cuts at: 0 601 1731
Size: 601 1130
SanDI GG'GwC_CC
Cuts at: 0 78 1731
Size: 78 1653
SbfI CC_TGCA'GG
Cuts at: 0 601 1731
Size: 601 1130
SfcI C'TryA_G
Cuts at: 0 101 597 1731
Size: 101 496 1134
Fragments arranged by size:
1134 496 101
SmaI CCC'GGG
Cuts at: 0 17 1731
Size: 17 1714
SmlI C'TyrA_G
Cuts at: 0 393 1441 1731
Size: 393 1048 290
Fragments arranged by size:
1048 393 290
StuI AGG'CCT
Cuts at: 0 888 972 1056 1140 1392 1560 1644 1731
Size: 888 84 84 84 252 168 84 87
Fragments arranged by size:
888 252 168 87 84 84 84 84
StyI C'CwwG_G
Cuts at: 0 423 1731
Size: 423 1308
TatI w'GTAC_w
Cuts at: 0 158 209 1731
Size: 158 51 1522
Fragments arranged by size:
1522 158 51
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 302 334 1731
Size: 302 32 1397
Fragments arranged by size:
1397 302 32
XmaI C'CCGG_G
Cuts at: 0 15 1731
Size: 15 1716
XmnI GAAnn'nnTTC
Cuts at: 0 616 1731
Size: 616 1115
Enzymes that do cut:
AcuI AflII AflIII AleI ApoI AvaI BanI Bme1580I
BmrI BpmI BpuEI BsaHI BsaWI BsaXI BsgI BsiEI
BsmI Bsp1286I BspHI BspMI BsrDI BstYI BtgZI BtsI
CspCI EaeI EciI Eco57MI EcoNI EcoO109I Hin4I MmeI
MscI MslI NspI PasI PciI PpuMI PstI SanDI
SbfI SfcI SmaI SmlI StuI StyI TatI TstI
XmaI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AgeI AhdI
AlfI AloI AlwNI ApaI ApaLI AscI AseI AsiSI
AvrII BaeI BamHI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BlpI BmgBI BmtI
BplI Bpu10I BsaI BsaAI BsaBI BseRI BseYI BsiHKAI
BsiWI BsmBI BspEI BsrBI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstZ17I Bsu36I BtgI ClaI
DraI DraIII DrdI EagI EarI EcoICRI EcoRI EcoRV
FalI FseI FspI FspAI HaeII HincII HindIII HpaI
KasI KpnI MfeI MluI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI NsiI PacI PflMI
PfoI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI
PsrI PvuI PvuII RsrII SacI SacII SalI SapI
ScaI SexAI SfiI SfoI SgfI SgrAI SnaBI SpeI
SphI SrfI SspI SwaI TaqII Tth111I XbaI XcmI
XhoI ZraI