Restriction map for
pFN21AE3249
(Linear) (Six-base) MAPSORT of: KIEE3249.seq Check: 1998 from: 1 to: 1653
KIEE3249.orf
With 182 enzymes: SgfI *
March 22, 2012 16:27 ..
AatII G_ACGT'C
Cuts at: 0 859 1653
Size: 859 794
Acc65I G'GTAC_C
Cuts at: 0 960 1653
Size: 960 693
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1624 1653
Size: 1624 29
AflIII A'CryG_T
Cuts at: 0 256 1158 1653
Size: 256 902 495
Fragments arranged by size:
902 495 256
AgeI A'CCGG_T
Cuts at: 0 1184 1653
Size: 1184 469
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1418 1653
Size: 1418 235
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 124 156 1653
Size: 124 32 1497
Fragments arranged by size:
1497 124 32
AlwNI CAG_nnn'CTG
Cuts at: 0 617 1653
Size: 617 1036
ApoI r'AATT_y
Cuts at: 0 230 1653
Size: 230 1423
AvaI C'yCGr_G
Cuts at: 0 1554 1653
Size: 1554 99
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 952 985 1653
Size: 952 33 668
Fragments arranged by size:
952 668 33
BanI G'GyrC_C
Cuts at: 0 960 1477 1653
Size: 960 517 176
Fragments arranged by size:
960 517 176
BanII G_rGCy'C
Cuts at: 0 137 713 1653
Size: 137 576 940
Fragments arranged by size:
940 576 137
BbsI GAAGACnn'nnnn_
Cuts at: 0 644 878 1653
Size: 644 234 775
Fragments arranged by size:
775 644 234
BbvCI CC'TCA_GC
Cuts at: 0 1372 1653
Size: 1372 281
BfrBI ATG'CAT
Cuts at: 0 1090 1401 1653
Size: 1090 311 252
Fragments arranged by size:
1090 311 252
BglI GCCn_nnn'nGGC
Cuts at: 0 1327 1653
Size: 1327 326
BglII A'GATC_T
Cuts at: 0 1200 1653
Size: 1200 453
BlpI GC'TnA_GC
Cuts at: 0 1127 1653
Size: 1127 526
Bme1580I G_kGCm'C
Cuts at: 0 827 1340 1653
Size: 827 513 313
Fragments arranged by size:
827 513 313
BmrI ACTGGGnnnn_n'
Cuts at: 0 132 1653
Size: 132 1521
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 177 1653
Size: 177 1476
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 385 710 1653
Size: 385 325 943
Fragments arranged by size:
943 385 325
Bpu10I CC'TnA_GC
Cuts at: 0 1194 1372 1653
Size: 1194 178 281
Fragments arranged by size:
1194 281 178
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 489 1653
Size: 489 1164
BsaI GGTCTCn'nnnn_
Cuts at: 0 376 560 725 1193 1653
Size: 376 184 165 468 460
Fragments arranged by size:
468 460 376 184 165
BsaAI yAC'GTr
Cuts at: 0 257 1653
Size: 257 1396
BsaBI GATnn'nnATC
Cuts at: 0 320 1653
Size: 320 1333
BsaHI Gr'CG_yC
Cuts at: 0 856 932 1653
Size: 856 76 721
Fragments arranged by size:
856 721 76
BsaWI w'CCGG_w
Cuts at: 0 1184 1225 1653
Size: 1184 41 428
Fragments arranged by size:
1184 428 41
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 124 154 1653
Size: 124 30 1499
Fragments arranged by size:
1499 124 30
BseYI C'CCAG_C
Cuts at: 0 482 602 1653
Size: 482 120 1051
Fragments arranged by size:
1051 482 120
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 89 1653
Size: 89 1564
BsiEI CG_ry'CG
Cuts at: 0 761 1653
Size: 761 892
Bsp1286I G_dGCh'C
Cuts at: 0 137 713 827 1340 1653
Size: 137 576 114 513 313
Fragments arranged by size:
576 513 313 137 114
BspHI T'CATG_A
Cuts at: 0 1640 1653
Size: 1640 13
BsrDI GCAATG_nn'
Cuts at: 0 1289 1523 1653
Size: 1289 234 130
Fragments arranged by size:
1289 234 130
BsrFI r'CCGG_y
Cuts at: 0 1184 1653
Size: 1184 469
BsrGI T'GTAC_A
Cuts at: 0 207 1506 1653
Size: 207 1299 147
Fragments arranged by size:
1299 207 147
BstEII G'GTnAC_C
Cuts at: 0 1221 1653
Size: 1221 432
BstXI CCAn_nnnn'nTGG
Cuts at: 0 935 1296 1653
Size: 935 361 357
Fragments arranged by size:
935 361 357
BstYI r'GATC_y
Cuts at: 0 1110 1200 1653
Size: 1110 90 453
Fragments arranged by size:
1110 453 90
Bsu36I CC'TnA_GG
Cuts at: 0 1488 1653
Size: 1488 165
BtgI C'CryG_G
Cuts at: 0 956 1176 1322 1653
Size: 956 220 146 331
Fragments arranged by size:
956 331 220 146
BtsI GCAGTG_nn'
Cuts at: 0 66 1653
Size: 66 1587
DraI TTT'AAA
Cuts at: 0 224 1653
Size: 224 1429
EaeI y'GGCC_r
Cuts at: 0 1165 1274 1653
Size: 1165 109 379
Fragments arranged by size:
1165 379 109
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 385 710 1624 1653
Size: 385 325 914 29
Fragments arranged by size:
914 385 325 29
EcoO109I rG'GnC_Cy
Cuts at: 0 93 438 940 1245 1653
Size: 93 345 502 305 408
Fragments arranged by size:
502 408 345 305 93
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 508 540 1432 1464 1653
Size: 508 32 892 32 189
Fragments arranged by size:
892 508 189 32 32
HincII GTy'rAC
Cuts at: 0 303 369 1653
Size: 303 66 1284
Fragments arranged by size:
1284 303 66
HpaI GTT'AAC
Cuts at: 0 369 1653
Size: 369 1284
KpnI G_GTAC'C
Cuts at: 0 964 1653
Size: 964 689
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 30 1653
Size: 30 1623
MscI TGG'CCA
Cuts at: 0 1167 1276 1653
Size: 1167 109 377
Fragments arranged by size:
1167 377 109
MslI CAynn'nnrTG
Cuts at: 0 1423 1653
Size: 1423 230
NcoI C'CATG_G
Cuts at: 0 956 1176 1322 1653
Size: 956 220 146 331
Fragments arranged by size:
956 331 220 146
NsiI A_TGCA'T
Cuts at: 0 1092 1403 1653
Size: 1092 311 250
Fragments arranged by size:
1092 311 250
NspI r_CATG'y
Cuts at: 0 1162 1405 1422 1653
Size: 1162 243 17 231
Fragments arranged by size:
1162 243 231 17
PasI CC'CwG_GG
Cuts at: 0 748 1653
Size: 748 905
PciI A'CATG_T
Cuts at: 0 1158 1653
Size: 1158 495
PfoI T'CCnGG_A
Cuts at: 0 687 1653
Size: 687 966
PmlI CAC'GTG
Cuts at: 0 257 1653
Size: 257 1396
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 124 156 1653
Size: 124 32 1497
Fragments arranged by size:
1497 124 32
PpuMI rG'GwC_Cy
Cuts at: 0 93 1245 1653
Size: 93 1152 408
Fragments arranged by size:
1152 408 93
ScaI AGT'ACT
Cuts at: 0 1350 1653
Size: 1350 303
SfcI C'TryA_G
Cuts at: 0 1561 1653
Size: 1561 92
SmlI C'TyrA_G
Cuts at: 0 468 1653
Size: 468 1185
SphI G_CATG'C
Cuts at: 0 1405 1422 1653
Size: 1405 17 231
Fragments arranged by size:
1405 231 17
StuI AGG'CCT
Cuts at: 0 1370 1653
Size: 1370 283
StyI C'CwwG_G
Cuts at: 0 735 956 1176 1322 1653
Size: 735 221 220 146 331
Fragments arranged by size:
735 331 221 220 146
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 28 305 1450 1465 1653
Size: 28 277 1145 15 188
Fragments arranged by size:
1145 277 188 28 15
TatI w'GTAC_w
Cuts at: 0 207 1348 1506 1653
Size: 207 1141 158 147
Fragments arranged by size:
1141 207 158 147
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 662 694 785 817 1653
Size: 662 32 91 32 836
Fragments arranged by size:
836 662 91 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 125 261 1269 1653
Size: 125 136 1008 384
Fragments arranged by size:
1008 384 136 125
XmnI GAAnn'nnTTC
Cuts at: 0 885 1653
Size: 885 768
ZraI GAC'GTC
Cuts at: 0 857 1653
Size: 857 796
Enzymes that do cut:
AatII Acc65I AcuI AflIII AgeI AlfI AloI AlwNI
ApoI AvaI BaeI BanI BanII BbsI BbvCI BfrBI
BglI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseYI
BsgI BsiEI Bsp1286I BspHI BsrDI BsrFI BsrGI BstEII
BstXI BstYI Bsu36I BtgI BtsI DraI EaeI Eco57MI
EcoO109I Hin4I HincII HpaI KpnI MmeI MscI MslI
NcoI NsiI NspI PasI PciI PfoI PmlI PpiI
PpuMI ScaI SfcI SmlI SphI StuI StyI TaqII
TatI TstI XcmI XmnI ZraI
Enzymes that do not cut:
AarI AccI AclI AfeI AflII AhdI AleI ApaI
ApaLI AscI AseI AsiSI AvrII BamHI BbeI BcgI
BciVI BclI BmgBI BmtI BseRI BsiHKAI BsiWI BsmI
BsmBI BspEI BspMI BsrBI BssHII BssSI BstAPI BstBI
BstZ17I BtgZI ClaI CspCI DraIII DrdI EagI EarI
EciI EcoICRI EcoNI EcoRI EcoRV FalI FseI FspI
FspAI HaeII HindIII KasI MfeI MluI MspA1I NaeI
NarI NdeI NgoMIV NheI NotI NruI PacI PflMI
PmeI PshAI PsiI PspOMI PspXI PsrI PstI PvuI
PvuII RsrII SacI SacII SalI SanDI SapI SbfI
SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI
SrfI SspI SwaI Tth111I XbaI XhoI XmaI