Restriction map for
pFN21AE3235
(Linear) (Six-base) MAPSORT of: KIEE3235.seq Check: 6161 from: 1 to: 1644
KIEE3235.orf
With 182 enzymes: SgfI *
March 22, 2012 16:26 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 535 1000 1644
Size: 535 465 644
Fragments arranged by size:
644 535 465
AgeI A'CCGG_T
Cuts at: 0 1183 1644
Size: 1183 461
AleI CACnn'nnGTG
Cuts at: 0 945 1186 1519 1644
Size: 945 241 333 125
Fragments arranged by size:
945 333 241 125
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 963 1166 1264 1644
Size: 963 203 98 380
Fragments arranged by size:
963 380 203 98
ApaLI G'TGCA_C
Cuts at: 0 886 1471 1644
Size: 886 585 173
Fragments arranged by size:
886 585 173
AvaI C'yCGr_G
Cuts at: 0 59 1297 1644
Size: 59 1238 347
Fragments arranged by size:
1238 347 59
BanI G'GyrC_C
Cuts at: 0 537 1258 1644
Size: 537 721 386
Fragments arranged by size:
721 537 386
BanII G_rGCy'C
Cuts at: 0 656 1223 1298 1304 1644
Size: 656 567 75 6 340
Fragments arranged by size:
656 567 340 75 6
BbsI GAAGACnn'nnnn_
Cuts at: 0 337 1644
Size: 337 1307
BclI T'GATC_A
Cuts at: 0 350 1644
Size: 350 1294
BfrBI ATG'CAT
Cuts at: 0 1489 1644
Size: 1489 155
BglI GCCn_nnn'nGGC
Cuts at: 0 1390 1644
Size: 1390 254
BglII A'GATC_T
Cuts at: 0 668 1433 1644
Size: 668 765 211
Fragments arranged by size:
765 668 211
BlpI GC'TnA_GC
Cuts at: 0 970 1644
Size: 970 674
Bme1580I G_kGCm'C
Cuts at: 0 157 374 542 890 1475 1644
Size: 157 217 168 348 585 169
Fragments arranged by size:
585 348 217 169 168 157
BmgBI CAC'GTC
Cuts at: 0 44 1248 1419 1644
Size: 44 1204 171 225
Fragments arranged by size:
1204 225 171 44
BmrI ACTGGGnnnn_n'
Cuts at: 0 167 1644
Size: 167 1477
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 919 1644
Size: 919 725
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 490 1644
Size: 490 1154
BsaI GGTCTCn'nnnn_
Cuts at: 0 88 1024 1644
Size: 88 936 620
Fragments arranged by size:
936 620 88
BsaAI yAC'GTr
Cuts at: 0 885 1344 1644
Size: 885 459 300
Fragments arranged by size:
885 459 300
BsaWI w'CCGG_w
Cuts at: 0 1183 1644
Size: 1183 461
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 670 700 1081 1111 1644
Size: 670 30 381 30 533
Fragments arranged by size:
670 533 381 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 916 1636 1644
Size: 916 720 8
Fragments arranged by size:
916 720 8
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 898 1344 1644
Size: 898 446 300
Fragments arranged by size:
898 446 300
BsiEI CG_ry'CG
Cuts at: 0 253 738 1581 1644
Size: 253 485 843 63
Fragments arranged by size:
843 485 253 63
BsiHKAI G_wGCw'C
Cuts at: 0 656 890 988 1091 1304 1406 1475 1644
Size: 656 234 98 103 213 102 69 169
Fragments arranged by size:
656 234 213 169 103 102 98 69
Bsp1286I G_dGCh'C
Cuts at: 0 157 374 542 656 890 988 1091 1223
Size: 157 217 168 114 234 98 103 132
Cuts at: 1223 1298 1304 1406 1475 1644
Size: 75 6 102 69 169
Fragments arranged by size:
234 217 169 168 157 132 114 103
102 98 75 69 6
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 984 1644
Size: 984 660
BsrBI CCG'CTC
Cuts at: 0 848 1644
Size: 848 796
BsrDI GCAATG_nn'
Cuts at: 0 123 1644
Size: 123 1521
BsrFI r'CCGG_y
Cuts at: 0 74 1183 1204 1644
Size: 74 1109 21 440
Fragments arranged by size:
1109 440 74 21
BssHII G'CGCG_C
Cuts at: 0 1200 1230 1644
Size: 1200 30 414
Fragments arranged by size:
1200 414 30
BstAPI GCAn_nnn'nTGC
Cuts at: 0 885 1644
Size: 885 759
BstXI CCAn_nnnn'nTGG
Cuts at: 0 306 819 1521 1644
Size: 306 513 702 123
Fragments arranged by size:
702 513 306 123
BstYI r'GATC_y
Cuts at: 0 668 1433 1644
Size: 668 765 211
Fragments arranged by size:
765 668 211
Bsu36I CC'TnA_GG
Cuts at: 0 1312 1424 1644
Size: 1312 112 220
Fragments arranged by size:
1312 220 112
BtgI C'CryG_G
Cuts at: 0 392 1214 1370 1514 1644
Size: 392 822 156 144 130
Fragments arranged by size:
822 392 156 144 130
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1349 1644
Size: 1349 295
BtsI GCAGTG_nn'
Cuts at: 0 324 1400 1620 1644
Size: 324 1076 220 24
Fragments arranged by size:
1076 324 220 24
DraIII CAC_nnn'GTG
Cuts at: 0 1419 1479 1644
Size: 1419 60 165
Fragments arranged by size:
1419 165 60
DrdI GACnn_nn'nnGTC
Cuts at: 0 832 1531 1644
Size: 832 699 113
Fragments arranged by size:
832 699 113
EaeI y'GGCC_r
Cuts at: 0 250 341 735 860 1382 1644
Size: 250 91 394 125 522 262
Fragments arranged by size:
522 394 262 250 125 91
EagI C'GGCC_G
Cuts at: 0 250 735 1644
Size: 250 485 909
Fragments arranged by size:
909 485 250
EarI CTCTTCn'nnn_
Cuts at: 0 520 976 1644
Size: 520 456 668
Fragments arranged by size:
668 520 456
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 685 1644
Size: 685 959
EcoICRI GAG'CTC
Cuts at: 0 654 1302 1644
Size: 654 648 342
Fragments arranged by size:
654 648 342
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 535 919 1000 1644
Size: 535 384 81 644
Fragments arranged by size:
644 535 384 81
EcoO109I rG'GnC_Cy
Cuts at: 0 360 1644
Size: 360 1284
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 804 1644
Size: 804 840
FspI TGC'GCA
Cuts at: 0 1252 1644
Size: 1252 392
FspAI rTGC'GCAy
Cuts at: 0 1252 1644
Size: 1252 392
HaeII r_GCGC'y
Cuts at: 0 165 1644
Size: 165 1479
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 670 702 1490 1522 1644
Size: 670 32 788 32 122
Fragments arranged by size:
788 670 122 32 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 629 713 1644
Size: 629 84 931
Fragments arranged by size:
931 629 84
MscI TGG'CCA
Cuts at: 0 343 862 1644
Size: 343 519 782
Fragments arranged by size:
782 519 343
MslI CAynn'nnrTG
Cuts at: 0 470 945 1186 1519 1644
Size: 470 475 241 333 125
Fragments arranged by size:
475 470 333 241 125
MspA1I CmG'CkG
Cuts at: 0 897 957 1644
Size: 897 60 687
Fragments arranged by size:
897 687 60
NaeI GCC'GGC
Cuts at: 0 76 1644
Size: 76 1568
NcoI C'CATG_G
Cuts at: 0 392 1214 1644
Size: 392 822 430
Fragments arranged by size:
822 430 392
NgoMIV G'CCGG_C
Cuts at: 0 74 1644
Size: 74 1570
NotI GC'GGCC_GC
Cuts at: 0 250 1644
Size: 250 1394
NruI TCG'CGA
Cuts at: 0 1040 1644
Size: 1040 604
NsiI A_TGCA'T
Cuts at: 0 1491 1644
Size: 1491 153
NspI r_CATG'y
Cuts at: 0 1008 1644
Size: 1008 636
PasI CC'CwG_GG
Cuts at: 0 374 1644
Size: 374 1270
PflMI CCAn_nnn'nTGG
Cuts at: 0 1564 1644
Size: 1564 80
PfoI T'CCnGG_A
Cuts at: 0 656 1406 1644
Size: 656 750 238
Fragments arranged by size:
750 656 238
PmlI CAC'GTG
Cuts at: 0 885 1344 1644
Size: 885 459 300
Fragments arranged by size:
885 459 300
PvuII CAG'CTG
Cuts at: 0 897 1644
Size: 897 747
SacI G_AGCT'C
Cuts at: 0 656 1304 1644
Size: 656 648 340
Fragments arranged by size:
656 648 340
SapI GCTCTTCn'nnn_
Cuts at: 0 976 1644
Size: 976 668
SgrAI Cr'CCGG_yG
Cuts at: 0 1183 1204 1644
Size: 1183 21 440
Fragments arranged by size:
1183 440 21
SmlI C'TyrA_G
Cuts at: 0 505 1644
Size: 505 1139
SphI G_CATG'C
Cuts at: 0 1008 1644
Size: 1008 636
StuI AGG'CCT
Cuts at: 0 292 1644
Size: 292 1352
StyI C'CwwG_G
Cuts at: 0 392 1214 1322 1644
Size: 392 822 108 322
Fragments arranged by size:
822 392 322 108
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 366 482 1644
Size: 366 116 1162
Fragments arranged by size:
1162 366 116
TatI w'GTAC_w
Cuts at: 0 764 797 1644
Size: 764 33 847
Fragments arranged by size:
847 764 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1081 1113 1644
Size: 1081 32 531
Fragments arranged by size:
1081 531 32
Tth111I GACn'n_nGTC
Cuts at: 0 1120 1600 1644
Size: 1120 480 44
Fragments arranged by size:
1120 480 44
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 816 1644
Size: 816 828
Enzymes that do cut:
AcuI AgeI AleI AlfI ApaLI AvaI BanI BanII
BbsI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BpmI BpuEI BsaI BsaAI BsaWI BsaXI BseRI
BsgI BsiEI BsiHKAI Bsp1286I BspMI BsrBI BsrDI BsrFI
BssHII BstAPI BstXI BstYI Bsu36I BtgI BtgZI BtsI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I FalI FspI FspAI HaeII Hin4I MmeI MscI
MslI MspA1I NaeI NcoI NgoMIV NotI NruI NsiI
NspI PasI PflMI PfoI PmlI PvuII SacI SapI
SgrAI SmlI SphI StuI StyI TaqII TatI TstI
Tth111I XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AhdI AloI AlwNI ApaI ApoI AscI AseI AsiSI
AvrII BaeI BamHI BbeI BbvCI BcgI BciVI BmtI
BplI Bpu10I BsaBI BsaHI BseYI BsiWI BsmI BsmBI
BspEI BspHI BsrGI BssSI BstBI BstEII BstZ17I ClaI
CspCI DraI EcoNI EcoRI EcoRV FseI HincII HindIII
HpaI KasI KpnI MfeI MluI NarI NdeI NheI
PacI PciI PmeI PpiI PpuMI PshAI PsiI PspOMI
PspXI PsrI PstI PvuI RsrII SacII SalI SanDI
SbfI ScaI SexAI SfcI SfiI SfoI SgfI SmaI
SnaBI SpeI SrfI SspI SwaI XbaI XhoI XmaI
XmnI ZraI