Restriction map for
pFN21AE3232
(Linear) (Six-base) MAPSORT of: KIEE3232.seq Check: 9668 from: 1 to: 1620
KIEE3232.orf
With 182 enzymes: SgfI *
July 3, 2012 17:29 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1197 1620
Size: 1197 423
AccI GT'mk_AC
Cuts at: 0 608 1620
Size: 608 1012
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 324 1620
Size: 324 1296
AflIII A'CryG_T
Cuts at: 0 689 1064 1620
Size: 689 375 556
Fragments arranged by size:
689 556 375
AgeI A'CCGG_T
Cuts at: 0 70 1620
Size: 70 1550
AleI CACnn'nnGTG
Cuts at: 0 211 526 1620
Size: 211 315 1094
Fragments arranged by size:
1094 315 211
ApaI G_GGCC'C
Cuts at: 0 1360 1620
Size: 1360 260
AvaI C'yCGr_G
Cuts at: 0 209 649 1620
Size: 209 440 971
Fragments arranged by size:
971 440 209
BanI G'GyrC_C
Cuts at: 0 67 373 531 1279 1620
Size: 67 306 158 748 341
Fragments arranged by size:
748 341 306 158 67
BanII G_rGCy'C
Cuts at: 0 872 1043 1360 1620
Size: 872 171 317 260
Fragments arranged by size:
872 317 260 171
BbeI G_GCGC'C
Cuts at: 0 377 535 1620
Size: 377 158 1085
Fragments arranged by size:
1085 377 158
BbsI GAAGACnn'nnnn_
Cuts at: 0 660 740 1620
Size: 660 80 880
Fragments arranged by size:
880 660 80
BclI T'GATC_A
Cuts at: 0 773 1620
Size: 773 847
BlpI GC'TnA_GC
Cuts at: 0 935 1620
Size: 935 685
Bme1580I G_kGCm'C
Cuts at: 0 289 1282 1360 1620
Size: 289 993 78 260
Fragments arranged by size:
993 289 260 78
BmgBI CAC'GTC
Cuts at: 0 692 1620
Size: 692 928
BmrI ACTGGGnnnn_n'
Cuts at: 0 272 456 1620
Size: 272 184 1164
Fragments arranged by size:
1164 272 184
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 609 646 940 1620
Size: 609 37 294 680
Fragments arranged by size:
680 609 294 37
BsaI GGTCTCn'nnnn_
Cuts at: 0 706 1223 1620
Size: 706 517 397
Fragments arranged by size:
706 517 397
BsaHI Gr'CG_yC
Cuts at: 0 123 374 532 1620
Size: 123 251 158 1088
Fragments arranged by size:
1088 251 158 123
BsaWI w'CCGG_w
Cuts at: 0 70 800 1620
Size: 70 730 820
Fragments arranged by size:
820 730 70
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 691 926 1087 1620
Size: 691 235 161 533
Fragments arranged by size:
691 533 235 161
BseYI C'CCAG_C
Cuts at: 0 47 469 1544 1620
Size: 47 422 1075 76
Fragments arranged by size:
1075 422 76 47
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 584 1620
Size: 584 1036
BsiEI CG_ry'CG
Cuts at: 0 220 1336 1620
Size: 220 1116 284
Fragments arranged by size:
1116 284 220
BsiHKAI G_wGCw'C
Cuts at: 0 20 872 1620
Size: 20 852 748
Fragments arranged by size:
852 748 20
BsmBI CGTCTCn'nnnn_
Cuts at: 0 11 1620
Size: 11 1609
Bsp1286I G_dGCh'C
Cuts at: 0 20 289 872 1043 1282 1360 1620
Size: 20 269 583 171 239 78 260
Fragments arranged by size:
583 269 260 239 171 78 20
BspEI T'CCGG_A
Cuts at: 0 800 1620
Size: 800 820
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 461 1197 1620
Size: 461 736 423
Fragments arranged by size:
736 461 423
BsrBI CCG'CTC
Cuts at: 0 630 1620
Size: 630 990
BsrFI r'CCGG_y
Cuts at: 0 61 70 219 1620
Size: 61 9 149 1401
Fragments arranged by size:
1401 149 61 9
BssSI C'ACGA_G
Cuts at: 0 661 1620
Size: 661 959
BstXI CCAn_nnnn'nTGG
Cuts at: 0 723 1620
Size: 723 897
BstYI r'GATC_y
Cuts at: 0 726 1600 1620
Size: 726 874 20
Fragments arranged by size:
874 726 20
BtgI C'CryG_G
Cuts at: 0 1007 1620
Size: 1007 613
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 425 1620
Size: 425 1195
BtsI GCAGTG_nn'
Cuts at: 0 795 1451 1620
Size: 795 656 169
Fragments arranged by size:
795 656 169
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1407 1442 1620
Size: 1407 35 178
Fragments arranged by size:
1407 178 35
DraI TTT'AAA
Cuts at: 0 1364 1620
Size: 1364 256
EaeI y'GGCC_r
Cuts at: 0 59 217 221 479 1333 1620
Size: 59 158 4 258 854 287
Fragments arranged by size:
854 287 258 158 59 4
EagI C'GGCC_G
Cuts at: 0 217 1333 1620
Size: 217 1116 287
Fragments arranged by size:
1116 287 217
EarI CTCTTCn'nnn_
Cuts at: 0 343 877 888 901 1620
Size: 343 534 11 13 719
Fragments arranged by size:
719 534 343 13 11
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 134 565 1620
Size: 134 431 1055
Fragments arranged by size:
1055 431 134
EcoICRI GAG'CTC
Cuts at: 0 870 1620
Size: 870 750
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 324 609 646 940 1620
Size: 324 285 37 294 680
Fragments arranged by size:
680 324 294 285 37
EcoO109I rG'GnC_Cy
Cuts at: 0 450 1356 1357 1620
Size: 450 906 1 263
Fragments arranged by size:
906 450 263 1
FseI GG_CCGG'CC
Cuts at: 0 223 1620
Size: 223 1397
FspI TGC'GCA
Cuts at: 0 241 745 1620
Size: 241 504 875
Fragments arranged by size:
875 504 241
HaeII r_GCGC'y
Cuts at: 0 142 377 424 478 535 1620
Size: 142 235 47 54 57 1085
Fragments arranged by size:
1085 235 142 57 54 47
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 758 790 951 983 1620
Size: 758 32 161 32 637
Fragments arranged by size:
758 637 161 32 32
HincII GTy'rAC
Cuts at: 0 1062 1620
Size: 1062 558
HpaI GTT'AAC
Cuts at: 0 1062 1620
Size: 1062 558
KasI G'GCGC_C
Cuts at: 0 373 531 1620
Size: 373 158 1089
Fragments arranged by size:
1089 373 158
MslI CAynn'nnrTG
Cuts at: 0 211 526 814 827 1012 1620
Size: 211 315 288 13 185 608
Fragments arranged by size:
608 315 288 211 185 13
MspA1I CmG'CkG
Cuts at: 0 484 1620
Size: 484 1136
NaeI GCC'GGC
Cuts at: 0 63 221 1620
Size: 63 158 1399
Fragments arranged by size:
1399 158 63
NarI GG'CG_CC
Cuts at: 0 374 532 1620
Size: 374 158 1088
Fragments arranged by size:
1088 374 158
NcoI C'CATG_G
Cuts at: 0 1007 1620
Size: 1007 613
NgoMIV G'CCGG_C
Cuts at: 0 61 219 1620
Size: 61 158 1401
Fragments arranged by size:
1401 158 61
NspI r_CATG'y
Cuts at: 0 1068 1620
Size: 1068 552
PciI A'CATG_T
Cuts at: 0 1064 1620
Size: 1064 556
PfoI T'CCnGG_A
Cuts at: 0 671 1620
Size: 671 949
PpuMI rG'GwC_Cy
Cuts at: 0 450 1620
Size: 450 1170
PspOMI G'GGCC_C
Cuts at: 0 1356 1620
Size: 1356 264
PspXI vC'TCGA_Gb
Cuts at: 0 649 1620
Size: 649 971
SacI G_AGCT'C
Cuts at: 0 872 1620
Size: 872 748
SapI GCTCTTCn'nnn_
Cuts at: 0 343 877 888 1620
Size: 343 534 11 732
Fragments arranged by size:
732 534 343 11
ScaI AGT'ACT
Cuts at: 0 511 1124 1620
Size: 511 613 496
Fragments arranged by size:
613 511 496
SfcI C'TryA_G
Cuts at: 0 609 699 1620
Size: 609 90 921
Fragments arranged by size:
921 609 90
SfoI GGC'GCC
Cuts at: 0 375 533 1620
Size: 375 158 1087
Fragments arranged by size:
1087 375 158
SgrAI Cr'CCGG_yG
Cuts at: 0 70 1620
Size: 70 1550
SmaI CCC'GGG
Cuts at: 0 211 1620
Size: 211 1409
SmlI C'TyrA_G
Cuts at: 0 649 1620
Size: 649 971
StyI C'CwwG_G
Cuts at: 0 1007 1351 1563 1620
Size: 1007 344 212 57
Fragments arranged by size:
1007 344 212 57
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1247 1297 1620
Size: 1247 50 323
Fragments arranged by size:
1247 323 50
TatI w'GTAC_w
Cuts at: 0 509 1122 1620
Size: 509 613 498
Fragments arranged by size:
613 509 498
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1445 1477 1620
Size: 1445 32 143
Fragments arranged by size:
1445 143 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 765 1266 1291 1620
Size: 765 501 25 329
Fragments arranged by size:
765 501 329 25
XhoI C'TCGA_G
Cuts at: 0 649 1620
Size: 649 971
XmaI C'CCGG_G
Cuts at: 0 209 1620
Size: 209 1411
Enzymes that do cut:
AarI AccI AcuI AflIII AgeI AleI ApaI AvaI
BanI BanII BbeI BbsI BclI BlpI Bme1580I BmgBI
BmrI BpmI BsaI BsaHI BsaWI BseRI BseYI BsgI
BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspMI BsrBI BsrFI
BssSI BstXI BstYI BtgI BtgZI BtsI CspCI DraI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I FseI
FspI HaeII Hin4I HincII HpaI KasI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PciI PfoI PpuMI
PspOMI PspXI SacI SapI ScaI SfcI SfoI SgrAI
SmaI SmlI StyI TaqII TatI TstI XcmI XhoI
XmaI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AhdI AlfI AloI
AlwNI ApaLI ApoI AscI AseI AsiSI AvrII BaeI
BamHI BbvCI BcgI BciVI BfrBI BglI BglII BmtI
BplI Bpu10I BpuEI BsaAI BsaBI BsaXI BsiWI BsmI
BspHI BsrDI BsrGI BssHII BstAPI BstBI BstEII BstZ17I
Bsu36I ClaI DraIII DrdI EcoNI EcoRI EcoRV FalI
FspAI HindIII KpnI MfeI MluI MmeI MscI NdeI
NheI NotI NruI NsiI PacI PasI PflMI PmeI
PmlI PpiI PshAI PsiI PsrI PstI PvuI PvuII
RsrII SacII SalI SanDI SbfI SexAI SfiI SgfI
SnaBI SpeI SphI SrfI SspI StuI SwaI Tth111I
XbaI XmnI ZraI