Restriction map for
pFN21AE3231
(Linear) (Six-base) MAPSORT of: KIEE3231.seq Check: 2046 from: 1 to: 1617
KIEE3231.orf
With 182 enzymes: SgfI *
March 22, 2012 16:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 122 1617
Size: 122 1495
Acc65I G'GTAC_C
Cuts at: 0 107 1617
Size: 107 1510
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 931 1189 1564 1617
Size: 931 258 375 53
Fragments arranged by size:
931 375 258 53
AflII C'TTAA_G
Cuts at: 0 1392 1617
Size: 1392 225
AleI CACnn'nnGTG
Cuts at: 0 784 1617
Size: 784 833
AlwNI CAG_nnn'CTG
Cuts at: 0 594 887 906 996 1212 1380 1487 1617
Size: 594 293 19 90 216 168 107 130
Fragments arranged by size:
594 293 216 168 130 107 90 19
ApaI G_GGCC'C
Cuts at: 0 223 320 1617
Size: 223 97 1297
Fragments arranged by size:
1297 223 97
AvaI C'yCGr_G
Cuts at: 0 38 102 215 307 1448 1491 1535 1617
Size: 38 64 113 92 1141 43 44 82
Fragments arranged by size:
1141 113 92 82 64 44 43 38
BanI G'GyrC_C
Cuts at: 0 90 107 291 1313 1444 1617
Size: 90 17 184 1022 131 173
Fragments arranged by size:
1022 184 173 131 90 17
BanII G_rGCy'C
Cuts at: 0 39 103 223 254 320 1082 1096 1411
Size: 39 64 120 31 66 762 14 315
Cuts at: 1411 1617
Size: 206
Fragments arranged by size:
762 315 206 120 66 64 39 31
14
BbeI G_GCGC'C
Cuts at: 0 94 1617
Size: 94 1523
BbvCI CC'TCA_GC
Cuts at: 0 983 1617
Size: 983 634
BclI T'GATC_A
Cuts at: 0 1478 1617
Size: 1478 139
BglI GCCn_nnn'nGGC
Cuts at: 0 1253 1617
Size: 1253 364
Bme1580I G_kGCm'C
Cuts at: 0 223 320 1447 1617
Size: 223 97 1127 170
Fragments arranged by size:
1127 223 170 97
BmgBI CAC'GTC
Cuts at: 0 1259 1617
Size: 1259 358
BmtI G_CTAG'C
Cuts at: 0 724 1617
Size: 724 893
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 571 574 883 928 973 1249 1390 1534
Size: 571 3 309 45 45 276 141 144
Cuts at: 1534 1585 1606 1617
Size: 51 21 11
Fragments arranged by size:
571 309 276 144 141 51 45 45
21 11 3
Bpu10I CC'TnA_GC
Cuts at: 0 253 983 1617
Size: 253 730 634
Fragments arranged by size:
730 634 253
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 658 1359 1617
Size: 658 701 258
Fragments arranged by size:
701 658 258
BsaI GGTCTCn'nnnn_
Cuts at: 0 329 1617
Size: 329 1288
BsaHI Gr'CG_yC
Cuts at: 0 91 1617
Size: 91 1526
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 796 826 1617
Size: 796 30 791
Fragments arranged by size:
796 791 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 817 1090 1617
Size: 817 273 527
Fragments arranged by size:
817 527 273
BseYI C'CCAG_C
Cuts at: 0 9 223 329 876 893 1416 1612 1617
Size: 9 214 106 547 17 523 196 5
Fragments arranged by size:
547 523 214 196 106 17 9 5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 382 1617
Size: 382 1235
BsiEI CG_ry'CG
Cuts at: 0 65 272 504 1617
Size: 65 207 232 1113
Fragments arranged by size:
1113 232 207 65
BsiHKAI G_wGCw'C
Cuts at: 0 919 1082 1096 1429 1617
Size: 919 163 14 333 188
Fragments arranged by size:
919 333 188 163 14
BsmI GAATG_Cn'
Cuts at: 0 341 824 938 1617
Size: 341 483 114 679
Fragments arranged by size:
679 483 341 114
Bsp1286I G_dGCh'C
Cuts at: 0 39 103 223 254 320 919 1082 1096
Size: 39 64 120 31 66 599 163 14
Cuts at: 1096 1411 1429 1447 1617
Size: 315 18 18 170
Fragments arranged by size:
599 315 170 163 120 66 64 39
31 18 18 14
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 12 122 1108 1163 1617
Size: 12 110 986 55 454
Fragments arranged by size:
986 454 110 55 12
BsrBI CCG'CTC
Cuts at: 0 59 1617
Size: 59 1558
BsrFI r'CCGG_y
Cuts at: 0 83 288 503 919 1194 1265 1617
Size: 83 205 215 416 275 71 352
Fragments arranged by size:
416 352 275 215 205 83 71
BssHII G'CGCG_C
Cuts at: 0 183 421 1183 1617
Size: 183 238 762 434
Fragments arranged by size:
762 434 238 183
BstAPI GCAn_nnn'nTGC
Cuts at: 0 887 1617
Size: 887 730
BstXI CCAn_nnnn'nTGG
Cuts at: 0 786 1617
Size: 786 831
BstYI r'GATC_y
Cuts at: 0 1167 1209 1617
Size: 1167 42 408
Fragments arranged by size:
1167 408 42
Bsu36I CC'TnA_GG
Cuts at: 0 1438 1617
Size: 1438 179
BtgI C'CryG_G
Cuts at: 0 49 230 690 767 1140 1617
Size: 49 181 460 77 373 477
Fragments arranged by size:
477 460 373 181 77 49
BtsI GCAGTG_nn'
Cuts at: 0 871 1321 1617
Size: 871 450 296
Fragments arranged by size:
871 450 296
DraIII CAC_nnn'GTG
Cuts at: 0 477 630 696 1617
Size: 477 153 66 921
Fragments arranged by size:
921 477 153 66
DrdI GACnn_nn'nnGTC
Cuts at: 0 486 712 1617
Size: 486 226 905
Fragments arranged by size:
905 486 226
EaeI y'GGCC_r
Cuts at: 0 62 233 269 501 1344 1617
Size: 62 171 36 232 843 273
Fragments arranged by size:
843 273 232 171 62 36
EagI C'GGCC_G
Cuts at: 0 62 269 501 1617
Size: 62 207 232 1116
Fragments arranged by size:
1116 232 207 62
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 403 544 1617
Size: 403 141 1073
Fragments arranged by size:
1073 403 141
EcoICRI GAG'CTC
Cuts at: 0 1080 1094 1617
Size: 1080 14 523
Fragments arranged by size:
1080 523 14
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 571 574 883 928 931 973 1189 1249
Size: 571 3 309 45 3 42 216 60
Cuts at: 1249 1390 1534 1564 1585 1606 1617
Size: 141 144 30 21 21 11
Fragments arranged by size:
571 309 216 144 141 60 45 42
30 21 21 11 3 3
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1372 1617
Size: 1372 245
EcoO109I rG'GnC_Cy
Cuts at: 0 219 220 316 812 903 1122 1365 1377
Size: 219 1 96 496 91 219 243 12
Cuts at: 1377 1560 1617
Size: 183 57
Fragments arranged by size:
496 243 219 219 183 96 91 57
12 1
FseI GG_CCGG'CC
Cuts at: 0 507 1617
Size: 507 1110
FspI TGC'GCA
Cuts at: 0 823 1617
Size: 823 794
HaeII r_GCGC'y
Cuts at: 0 49 94 230 1617
Size: 49 45 136 1387
Fragments arranged by size:
1387 136 49 45
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 796 828 1617
Size: 796 32 789
Fragments arranged by size:
796 789 32
KasI G'GCGC_C
Cuts at: 0 90 1617
Size: 90 1527
KpnI G_GTAC'C
Cuts at: 0 111 1617
Size: 111 1506
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 51 149 1617
Size: 51 98 1468
Fragments arranged by size:
1468 98 51
MscI TGG'CCA
Cuts at: 0 1346 1617
Size: 1346 271
MslI CAynn'nnrTG
Cuts at: 0 784 1617
Size: 784 833
MspA1I CmG'CkG
Cuts at: 0 51 180 232 437 495 784 884 951
Size: 51 129 52 205 58 289 100 67
Cuts at: 951 1487 1526 1593 1617
Size: 536 39 67 24
Fragments arranged by size:
536 289 205 129 100 67 67 58
52 51 39 24
NaeI GCC'GGC
Cuts at: 0 505 1196 1617
Size: 505 691 421
Fragments arranged by size:
691 505 421
NarI GG'CG_CC
Cuts at: 0 91 1617
Size: 91 1526
NcoI C'CATG_G
Cuts at: 0 767 1617
Size: 767 850
NgoMIV G'CCGG_C
Cuts at: 0 503 1194 1617
Size: 503 691 423
Fragments arranged by size:
691 503 423
NheI G'CTAG_C
Cuts at: 0 720 1617
Size: 720 897
NotI GC'GGCC_GC
Cuts at: 0 269 1617
Size: 269 1348
NspI r_CATG'y
Cuts at: 0 135 1617
Size: 135 1482
PasI CC'CwG_GG
Cuts at: 0 1402 1617
Size: 1402 215
PflMI CCAn_nnn'nTGG
Cuts at: 0 750 906 1617
Size: 750 156 711
Fragments arranged by size:
750 711 156
PfoI T'CCnGG_A
Cuts at: 0 905 1032 1050 1617
Size: 905 127 18 567
Fragments arranged by size:
905 567 127 18
PpuMI rG'GwC_Cy
Cuts at: 0 812 903 1122 1365 1617
Size: 812 91 219 243 252
Fragments arranged by size:
812 252 243 219 91
PspOMI G'GGCC_C
Cuts at: 0 219 316 1617
Size: 219 97 1301
Fragments arranged by size:
1301 219 97
PstI C_TGCA'G
Cuts at: 0 122 971 1617
Size: 122 849 646
Fragments arranged by size:
849 646 122
PvuII CAG'CTG
Cuts at: 0 784 884 951 1487 1593 1617
Size: 784 100 67 536 106 24
Fragments arranged by size:
784 536 106 100 67 24
SacI G_AGCT'C
Cuts at: 0 1082 1096 1617
Size: 1082 14 521
Fragments arranged by size:
1082 521 14
SacII CC_GC'GG
Cuts at: 0 52 233 1617
Size: 52 181 1384
Fragments arranged by size:
1384 181 52
SanDI GG'GwC_CC
Cuts at: 0 812 1617
Size: 812 805
SfcI C'TryA_G
Cuts at: 0 118 757 840 967 1617
Size: 118 639 83 127 650
Fragments arranged by size:
650 639 127 118 83
SfoI GGC'GCC
Cuts at: 0 92 1617
Size: 92 1525
SmaI CCC'GGG
Cuts at: 0 40 217 1450 1493 1537 1617
Size: 40 177 1233 43 44 80
Fragments arranged by size:
1233 177 80 44 43 40
SmlI C'TyrA_G
Cuts at: 0 673 1338 1392 1617
Size: 673 665 54 225
Fragments arranged by size:
673 665 225 54
SphI G_CATG'C
Cuts at: 0 135 1617
Size: 135 1482
SrfI GCCC'GGGC
Cuts at: 0 40 1493 1617
Size: 40 1453 124
Fragments arranged by size:
1453 124 40
StyI C'CwwG_G
Cuts at: 0 242 347 512 767 1280 1617
Size: 242 105 165 255 513 337
Fragments arranged by size:
513 337 255 242 165 105
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 309 1430 1617
Size: 309 1121 187
Fragments arranged by size:
1121 309 187
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 61 93 1028 1060 1561 1593 1617
Size: 61 32 935 32 501 32 24
Fragments arranged by size:
935 501 61 32 32 32 24
Tth111I GACn'n_nGTC
Cuts at: 0 483 1617
Size: 483 1134
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 405 1617
Size: 405 1212
XmaI C'CCGG_G
Cuts at: 0 38 215 1448 1491 1535 1617
Size: 38 177 1233 43 44 82
Fragments arranged by size:
1233 177 82 44 43 38
Enzymes that do cut:
AarI Acc65I AcuI AflII AleI AlwNI ApaI AvaI
BanI BanII BbeI BbvCI BclI BglI Bme1580I BmgBI
BmtI BpmI Bpu10I BpuEI BsaI BsaHI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspMI BsrBI
BsrFI BssHII BstAPI BstXI BstYI Bsu36I BtgI BtsI
DraIII DrdI EaeI EagI EciI EcoICRI Eco57MI EcoNI
EcoO109I FseI FspI HaeII Hin4I KasI KpnI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NheI
NotI NspI PasI PflMI PfoI PpuMI PspOMI PstI
PvuII SacI SacII SanDI SfcI SfoI SmaI SmlI
SphI SrfI StyI TaqII TstI Tth111I XcmI XmaI
Enzymes that do not cut:
AatII AccI AclI AfeI AflIII AgeI AhdI AlfI
AloI ApaLI ApoI AscI AseI AsiSI AvrII BaeI
BamHI BbsI BcgI BciVI BfrBI BglII BlpI BmrI
BplI BsaAI BsaBI BsaWI BsiWI BsmBI BspEI BspHI
BsrDI BsrGI BssSI BstBI BstEII BstZ17I BtgZI ClaI
CspCI DraI EarI EcoRI EcoRV FalI FspAI HincII
HindIII HpaI MfeI MluI NdeI NruI NsiI PacI
PciI PmeI PmlI PpiI PshAI PsiI PspXI PsrI
PvuI RsrII SalI SapI SbfI ScaI SexAI SfiI
SgfI SgrAI SnaBI SpeI SspI StuI SwaI TatI
XbaI XhoI XmnI ZraI