Restriction map for
pFN21AE3227
(Linear) (Six-base) MAPSORT of: KIEE3227.seq Check: 2560 from: 1 to: 1560
KIEE3227.orf
With 182 enzymes: SgfI *
March 22, 2012 16:23 ..
AccI GT'mk_AC
Cuts at: 0 1439 1560
Size: 1439 121
AclI AA'CG_TT
Cuts at: 0 638 1560
Size: 638 922
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1163 1358 1476 1560
Size: 1163 195 118 84
Fragments arranged by size:
1163 195 118 84
AflIII A'CryG_T
Cuts at: 0 23 552 1068 1560
Size: 23 529 516 492
Fragments arranged by size:
529 516 492 23
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 462 1560
Size: 462 1098
AlwNI CAG_nnn'CTG
Cuts at: 0 1210 1556 1560
Size: 1210 346 4
Fragments arranged by size:
1210 346 4
ApaI G_GGCC'C
Cuts at: 0 7 1560
Size: 7 1553
ApoI r'AATT_y
Cuts at: 0 136 451 1560
Size: 136 315 1109
Fragments arranged by size:
1109 315 136
BamHI G'GATC_C
Cuts at: 0 992 1560
Size: 992 568
BanII G_rGCy'C
Cuts at: 0 7 1560
Size: 7 1553
BbsI GAAGACnn'nnnn_
Cuts at: 0 1482 1560
Size: 1482 78
BglI GCCn_nnn'nGGC
Cuts at: 0 535 1560
Size: 535 1025
BglII A'GATC_T
Cuts at: 0 1136 1560
Size: 1136 424
BlpI GC'TnA_GC
Cuts at: 0 519 1560
Size: 519 1041
Bme1580I G_kGCm'C
Cuts at: 0 7 963 1560
Size: 7 956 597
Fragments arranged by size:
956 597 7
BmrI ACTGGGnnnn_n'
Cuts at: 0 310 1535 1560
Size: 310 1225 25
Fragments arranged by size:
1225 310 25
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 892 1560
Size: 892 668
Bpu10I CC'TnA_GC
Cuts at: 0 687 1560
Size: 687 873
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 442 464 483 1560
Size: 442 22 19 1077
Fragments arranged by size:
1077 442 22 19
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 467 1560
Size: 467 1093
BseYI C'CCAG_C
Cuts at: 0 51 467 1560
Size: 51 416 1093
Fragments arranged by size:
1093 416 51
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 48 103 1560
Size: 48 55 1457
Fragments arranged by size:
1457 55 48
BsmI GAATG_Cn'
Cuts at: 0 316 542 812 882 1394 1560
Size: 316 226 270 70 512 166
Fragments arranged by size:
512 316 270 226 166 70
Bsp1286I G_dGCh'C
Cuts at: 0 7 963 1560
Size: 7 956 597
Fragments arranged by size:
956 597 7
BsrDI GCAATG_nn'
Cuts at: 0 488 1560
Size: 488 1072
BsrFI r'CCGG_y
Cuts at: 0 32 1560
Size: 32 1528
BsrGI T'GTAC_A
Cuts at: 0 680 1560
Size: 680 880
BstAPI GCAn_nnn'nTGC
Cuts at: 0 373 1560
Size: 373 1187
BstEII G'GTnAC_C
Cuts at: 0 167 1560
Size: 167 1393
BstYI r'GATC_y
Cuts at: 0 992 1031 1136 1560
Size: 992 39 105 424
Fragments arranged by size:
992 424 105 39
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 705 1560
Size: 705 855
BtsI GCAGTG_nn'
Cuts at: 0 234 530 578 847 1560
Size: 234 296 48 269 713
Fragments arranged by size:
713 296 269 234 48
EaeI y'GGCC_r
Cuts at: 0 1160 1560
Size: 1160 400
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 892 1163 1358 1476 1560
Size: 892 271 195 118 84
Fragments arranged by size:
892 271 195 118 84
HaeII r_GCGC'y
Cuts at: 0 52 115 219 1560
Size: 52 63 104 1341
Fragments arranged by size:
1341 104 63 52
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 337 369 850 882 1560
Size: 337 32 481 32 678
Fragments arranged by size:
678 481 337 32 32
MscI TGG'CCA
Cuts at: 0 1162 1560
Size: 1162 398
MslI CAynn'nnrTG
Cuts at: 0 768 838 956 1046 1181 1367 1560
Size: 768 70 118 90 135 186 193
Fragments arranged by size:
768 193 186 135 118 90 70
MspA1I CmG'CkG
Cuts at: 0 1496 1560
Size: 1496 64
NaeI GCC'GGC
Cuts at: 0 34 1560
Size: 34 1526
NgoMIV G'CCGG_C
Cuts at: 0 32 1560
Size: 32 1528
NspI r_CATG'y
Cuts at: 0 556 767 843 1072 1560
Size: 556 211 76 229 488
Fragments arranged by size:
556 488 229 211 76
PciI A'CATG_T
Cuts at: 0 552 1068 1560
Size: 552 516 492
Fragments arranged by size:
552 516 492
PflMI CCAn_nnn'nTGG
Cuts at: 0 1002 1560
Size: 1002 558
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 502 534 1560
Size: 502 32 1026
Fragments arranged by size:
1026 502 32
PsiI TTA'TAA
Cuts at: 0 350 1560
Size: 350 1210
PspOMI G'GGCC_C
Cuts at: 0 3 1560
Size: 3 1557
PvuII CAG'CTG
Cuts at: 0 1496 1560
Size: 1496 64
ScaI AGT'ACT
Cuts at: 0 132 1560
Size: 132 1428
SfcI C'TryA_G
Cuts at: 0 173 705 791 1560
Size: 173 532 86 769
Fragments arranged by size:
769 532 173 86
SmlI C'TyrA_G
Cuts at: 0 421 462 479 1560
Size: 421 41 17 1081
Fragments arranged by size:
1081 421 41 17
SpeI A'CTAG_T
Cuts at: 0 1401 1560
Size: 1401 159
SphI G_CATG'C
Cuts at: 0 767 1560
Size: 767 793
SspI AAT'ATT
Cuts at: 0 825 1560
Size: 825 735
StyI C'CwwG_G
Cuts at: 0 901 1560
Size: 901 659
TatI w'GTAC_w
Cuts at: 0 130 680 1191 1560
Size: 130 550 511 369
Fragments arranged by size:
550 511 369 130
XbaI T'CTAG_A
Cuts at: 0 609 1017 1560
Size: 609 408 543
Fragments arranged by size:
609 543 408
Enzymes that do cut:
AccI AclI AcuI AflIII AlfI AlwNI ApaI ApoI
BamHI BanII BbsI BglI BglII BlpI Bme1580I BmrI
BpmI Bpu10I BpuEI BseRI BseYI BsgI BsmI Bsp1286I
BsrDI BsrFI BsrGI BstAPI BstEII BstYI BtgZI BtsI
EaeI Eco57MI HaeII Hin4I MscI MslI MspA1I NaeI
NgoMIV NspI PciI PflMI PpiI PsiI PspOMI PvuII
ScaI SfcI SmlI SpeI SphI SspI StyI TatI
XbaI
Enzymes that do not cut:
AarI AatII Acc65I AfeI AflII AgeI AhdI AleI
AloI ApaLI AscI AseI AsiSI AvaI AvrII BaeI
BanI BbeI BbvCI BcgI BciVI BclI BfrBI BmgBI
BmtI BplI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BsiEI BsiHKAI BsiWI BsmBI BspEI BspHI BspMI BsrBI
BssHII BssSI BstBI BstXI BstZ17I Bsu36I BtgI ClaI
CspCI DraI DraIII DrdI EagI EarI EciI EcoICRI
EcoNI EcoO109I EcoRI EcoRV FalI FseI FspI FspAI
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
NarI NcoI NdeI NheI NotI NruI NsiI PacI
PasI PfoI PmeI PmlI PpuMI PshAI PspXI PsrI
PstI PvuI RsrII SacI SacII SalI SanDI SapI
SbfI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI
SrfI StuI SwaI TaqII TstI Tth111I XcmI XhoI
XmaI XmnI ZraI