Restriction map for
pFN21AE3225
(Linear) (Six-base) MAPSORT of: KIEE3225.seq Check: 8998 from: 1 to: 1551
KIEE3225.orf
With 182 enzymes: SgfI *
March 22, 2012 16:23 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 235 1551
Size: 235 1316
AclI AA'CG_TT
Cuts at: 0 883 1551
Size: 883 668
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1030 1551
Size: 1030 521
AfeI AGC'GCT
Cuts at: 0 55 1551
Size: 55 1496
AflII C'TTAA_G
Cuts at: 0 438 1551
Size: 438 1113
AhdI GACnn_n'nnGTC
Cuts at: 0 1357 1551
Size: 1357 194
AlwNI CAG_nnn'CTG
Cuts at: 0 981 1419 1551
Size: 981 438 132
Fragments arranged by size:
981 438 132
ApaI G_GGCC'C
Cuts at: 0 1188 1551
Size: 1188 363
AvaI C'yCGr_G
Cuts at: 0 11 542 1551
Size: 11 531 1009
Fragments arranged by size:
1009 531 11
BanI G'GyrC_C
Cuts at: 0 1213 1551
Size: 1213 338
BanII G_rGCy'C
Cuts at: 0 10 329 1158 1188 1551
Size: 10 319 829 30 363
Fragments arranged by size:
829 363 319 30 10
BbeI G_GCGC'C
Cuts at: 0 1217 1551
Size: 1217 334
BbsI GAAGACnn'nnnn_
Cuts at: 0 684 1551
Size: 684 867
BglI GCCn_nnn'nGGC
Cuts at: 0 22 43 1551
Size: 22 21 1508
Fragments arranged by size:
1508 22 21
BglII A'GATC_T
Cuts at: 0 458 1551
Size: 458 1093
BlpI GC'TnA_GC
Cuts at: 0 4 982 1551
Size: 4 978 569
Fragments arranged by size:
978 569 4
Bme1580I G_kGCm'C
Cuts at: 0 1188 1551
Size: 1188 363
BmrI ACTGGGnnnn_n'
Cuts at: 0 111 297 1264 1551
Size: 111 186 967 287
Fragments arranged by size:
967 287 186 111
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 315 348 1551
Size: 315 33 1203
Fragments arranged by size:
1203 315 33
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 294 595 1153 1458 1551
Size: 294 301 558 305 93
Fragments arranged by size:
558 305 301 294 93
BsaI GGTCTCn'nnnn_
Cuts at: 0 524 1431 1551
Size: 524 907 120
Fragments arranged by size:
907 524 120
BsaAI yAC'GTr
Cuts at: 0 1026 1551
Size: 1026 525
BsaHI Gr'CG_yC
Cuts at: 0 1214 1551
Size: 1214 337
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 568 598 1273 1276 1303 1306 1453 1483
Size: 568 30 675 3 27 3 147 30
Cuts at: 1483 1525 1551
Size: 42 26
Fragments arranged by size:
675 568 147 42 30 30 27 26
3 3
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1281 1551
Size: 1281 270
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1048 1551
Size: 1048 503
BsiEI CG_ry'CG
Cuts at: 0 38 77 1551
Size: 38 39 1474
Fragments arranged by size:
1474 39 38
BsiHKAI G_wGCw'C
Cuts at: 0 10 1025 1551
Size: 10 1015 526
Fragments arranged by size:
1015 526 10
BsmI GAATG_Cn'
Cuts at: 0 567 1551
Size: 567 984
Bsp1286I G_dGCh'C
Cuts at: 0 10 329 1025 1158 1188 1551
Size: 10 319 696 133 30 363
Fragments arranged by size:
696 363 319 133 30 10
BspHI T'CATG_A
Cuts at: 0 92 233 1551
Size: 92 141 1318
Fragments arranged by size:
1318 141 92
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 235 619 1493 1551
Size: 235 384 874 58
Fragments arranged by size:
874 384 235 58
BsrGI T'GTAC_A
Cuts at: 0 446 859 1551
Size: 446 413 692
Fragments arranged by size:
692 446 413
BstEII G'GTnAC_C
Cuts at: 0 626 781 1551
Size: 626 155 770
Fragments arranged by size:
770 626 155
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1075 1551
Size: 1075 476
BstYI r'GATC_y
Cuts at: 0 458 1551
Size: 458 1093
BtgI C'CryG_G
Cuts at: 0 32 996 1332 1551
Size: 32 964 336 219
Fragments arranged by size:
964 336 219 32
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 40 1551
Size: 40 1511
BtsI GCAGTG_nn'
Cuts at: 0 801 1551
Size: 801 750
EaeI y'GGCC_r
Cuts at: 0 35 74 1551
Size: 35 39 1477
Fragments arranged by size:
1477 39 35
EagI C'GGCC_G
Cuts at: 0 35 74 1551
Size: 35 39 1477
Fragments arranged by size:
1477 39 35
EarI CTCTTCn'nnn_
Cuts at: 0 284 1309 1551
Size: 284 1025 242
Fragments arranged by size:
1025 284 242
EcoICRI GAG'CTC
Cuts at: 0 8 1551
Size: 8 1543
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 294 595 1030 1153 1458 1551
Size: 294 301 435 123 305 93
Fragments arranged by size:
435 305 301 294 123 93
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1459 1551
Size: 1459 92
EcoO109I rG'GnC_Cy
Cuts at: 0 160 1123 1184 1185 1464 1551
Size: 160 963 61 1 279 87
Fragments arranged by size:
963 279 160 87 61 1
HaeII r_GCGC'y
Cuts at: 0 57 1217 1252 1481 1551
Size: 57 1160 35 229 70
Fragments arranged by size:
1160 229 70 57 35
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 74 106 443 475 568 600 1273 1305
Size: 74 32 337 32 93 32 673 32
Cuts at: 1305 1330 1343 1362 1375 1453 1485 1525 1551
Size: 25 13 19 13 78 32 40 26
Fragments arranged by size:
673 337 93 78 74 40 32 32
32 32 32 26 25 19 13 13
HincII GTy'rAC
Cuts at: 0 1093 1551
Size: 1093 458
KasI G'GCGC_C
Cuts at: 0 1213 1551
Size: 1213 338
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 410 1551
Size: 410 1141
MspA1I CmG'CkG
Cuts at: 0 34 978 1551
Size: 34 944 573
Fragments arranged by size:
944 573 34
NarI GG'CG_CC
Cuts at: 0 1214 1551
Size: 1214 337
NcoI C'CATG_G
Cuts at: 0 1332 1551
Size: 1332 219
NspI r_CATG'y
Cuts at: 0 1344 1551
Size: 1344 207
PasI CC'CwG_GG
Cuts at: 0 1120 1551
Size: 1120 431
PflMI CCAn_nnn'nTGG
Cuts at: 0 650 1551
Size: 650 901
PfoI T'CCnGG_A
Cuts at: 0 1510 1551
Size: 1510 41
PmlI CAC'GTG
Cuts at: 0 1026 1551
Size: 1026 525
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1384 1416 1551
Size: 1384 32 135
Fragments arranged by size:
1384 135 32
PpuMI rG'GwC_Cy
Cuts at: 0 160 1464 1551
Size: 160 1304 87
Fragments arranged by size:
1304 160 87
PspOMI G'GGCC_C
Cuts at: 0 1184 1551
Size: 1184 367
PstI C_TGCA'G
Cuts at: 0 1142 1460 1551
Size: 1142 318 91
Fragments arranged by size:
1142 318 91
RsrII CG'GwC_CG
Cuts at: 0 65 1551
Size: 65 1486
SacI G_AGCT'C
Cuts at: 0 10 1551
Size: 10 1541
SacII CC_GC'GG
Cuts at: 0 35 1551
Size: 35 1516
SanDI GG'GwC_CC
Cuts at: 0 160 1551
Size: 160 1391
SbfI CC_TGCA'GG
Cuts at: 0 1460 1551
Size: 1460 91
ScaI AGT'ACT
Cuts at: 0 307 1551
Size: 307 1244
SfcI C'TryA_G
Cuts at: 0 973 1138 1284 1456 1551
Size: 973 165 146 172 95
Fragments arranged by size:
973 172 165 146 95
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 43 1551
Size: 43 1508
SfoI GGC'GCC
Cuts at: 0 1215 1551
Size: 1215 336
SmaI CCC'GGG
Cuts at: 0 13 1551
Size: 13 1538
SmlI C'TyrA_G
Cuts at: 0 438 1551
Size: 438 1113
SpeI A'CTAG_T
Cuts at: 0 685 1551
Size: 685 866
StuI AGG'CCT
Cuts at: 0 254 1551
Size: 254 1297
StyI C'CwwG_G
Cuts at: 0 875 1074 1332 1551
Size: 875 199 258 219
Fragments arranged by size:
875 258 219 199
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 800 1551
Size: 800 751
TatI w'GTAC_w
Cuts at: 0 305 446 859 1551
Size: 305 141 413 692
Fragments arranged by size:
692 413 305 141
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 625 657 832 864 1551
Size: 625 32 175 32 687
Fragments arranged by size:
687 625 175 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 1438 1551
Size: 1438 113
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 773 1551
Size: 773 778
XmaI C'CCGG_G
Cuts at: 0 11 1551
Size: 11 1540
Enzymes that do cut:
AarI AclI AcuI AfeI AflII AhdI AlwNI ApaI
AvaI BanI BanII BbeI BbsI BglI BglII BlpI
Bme1580I BmrI BplI BpmI BsaI BsaAI BsaHI BsaXI
BseRI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspHI BspMI
BsrGI BstEII BstXI BstYI BtgI BtgZI BtsI EaeI
EagI EarI EcoICRI Eco57MI EcoNI EcoO109I HaeII Hin4I
HincII KasI MmeI MspA1I NarI NcoI NspI PasI
PflMI PfoI PmlI PpiI PpuMI PspOMI PstI RsrII
SacI SacII SanDI SbfI ScaI SfcI SfiI SfoI
SmaI SmlI SpeI StuI StyI TaqII TatI TstI
Tth111I XcmI XmaI
Enzymes that do not cut:
AatII AccI Acc65I AflIII AgeI AleI AlfI AloI
ApaLI ApoI AscI AseI AsiSI AvrII BaeI BamHI
BbvCI BcgI BciVI BclI BfrBI BmgBI BmtI Bpu10I
BpuEI BsaBI BsaWI BseYI BsiWI BsmBI BspEI BsrBI
BsrDI BsrFI BssHII BssSI BstAPI BstBI BstZ17I Bsu36I
ClaI CspCI DraI DraIII DrdI EciI EcoRI EcoRV
FalI FseI FspI FspAI HindIII HpaI KpnI MfeI
MluI MscI MslI NaeI NdeI NgoMIV NheI NotI
NruI NsiI PacI PciI PmeI PshAI PsiI PspXI
PsrI PvuI PvuII SalI SapI SexAI SgfI SgrAI
SnaBI SphI SrfI SspI SwaI XbaI XhoI XmnI
ZraI