Restriction map for
pFN21AE3222
(Linear) (Six-base) MAPSORT of: KIEE3222.seq Check: 6452 from: 1 to: 1512
KIEE3222.orf
With 182 enzymes: SgfI *
July 3, 2012 17:29 ..
AclI AA'CG_TT
Cuts at: 0 545 1512
Size: 545 967
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1302 1512
Size: 1302 210
AflII C'TTAA_G
Cuts at: 0 10 1512
Size: 10 1502
AleI CACnn'nnGTG
Cuts at: 0 160 1512
Size: 160 1352
AlwNI CAG_nnn'CTG
Cuts at: 0 894 1408 1512
Size: 894 514 104
Fragments arranged by size:
894 514 104
ApoI r'AATT_y
Cuts at: 0 307 326 790 1183 1512
Size: 307 19 464 393 329
Fragments arranged by size:
464 393 329 307 19
AvaI C'yCGr_G
Cuts at: 0 1100 1512
Size: 1100 412
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 371 404 663 696 1512
Size: 371 33 259 33 816
Fragments arranged by size:
816 371 259 33 33
BanII G_rGCy'C
Cuts at: 0 647 686 764 1433 1512
Size: 647 39 78 669 79
Fragments arranged by size:
669 647 79 78 39
BbsI GAAGACnn'nnnn_
Cuts at: 0 406 1512
Size: 406 1106
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1081 1115 1512
Size: 1081 34 397
Fragments arranged by size:
1081 397 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 664 1512
Size: 664 848
BlpI GC'TnA_GC
Cuts at: 0 1088 1512
Size: 1088 424
Bme1580I G_kGCm'C
Cuts at: 0 136 713 1512
Size: 136 577 799
Fragments arranged by size:
799 577 136
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 423 1512
Size: 423 1089
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 294 830 935 1040 1385 1512
Size: 294 536 105 105 345 127
Fragments arranged by size:
536 345 294 127 105 105
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 283 1512
Size: 283 1229
BsaI GGTCTCn'nnnn_
Cuts at: 0 67 1512
Size: 67 1445
BsaBI GATnn'nnATC
Cuts at: 0 497 943 1512
Size: 497 446 569
Fragments arranged by size:
569 497 446
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 34 64 359 389 1512
Size: 34 30 295 30 1123
Fragments arranged by size:
1123 295 34 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 772 1489 1512
Size: 772 717 23
Fragments arranged by size:
772 717 23
BseYI C'CCAG_C
Cuts at: 0 1269 1512
Size: 1269 243
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 13 577 1004 1512
Size: 13 564 427 508
Fragments arranged by size:
564 508 427 13
BsiHKAI G_wGCw'C
Cuts at: 0 686 764 1499 1512
Size: 686 78 735 13
Fragments arranged by size:
735 686 78 13
Bsp1286I G_dGCh'C
Cuts at: 0 136 647 686 713 764 1433 1499 1512
Size: 136 511 39 27 51 669 66 13
Fragments arranged by size:
669 511 136 66 51 39 27 13
BspHI T'CATG_A
Cuts at: 0 660 1512
Size: 660 852
BstBI TT'CG_AA
Cuts at: 0 1311 1512
Size: 1311 201
BstEII G'GTnAC_C
Cuts at: 0 451 1512
Size: 451 1061
BstXI CCAn_nnnn'nTGG
Cuts at: 0 113 1512
Size: 113 1399
BstYI r'GATC_y
Cuts at: 0 938 1512
Size: 938 574
BtgI C'CryG_G
Cuts at: 0 53 1359 1512
Size: 53 1306 153
Fragments arranged by size:
1306 153 53
BtsI GCAGTG_nn'
Cuts at: 0 25 1139 1512
Size: 25 1114 373
Fragments arranged by size:
1114 373 25
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 491 526 1512
Size: 491 35 986
Fragments arranged by size:
986 491 35
DraIII CAC_nnn'GTG
Cuts at: 0 1486 1512
Size: 1486 26
DrdI GACnn_nn'nnGTC
Cuts at: 0 194 1512
Size: 194 1318
EarI CTCTTCn'nnn_
Cuts at: 0 12 70 1512
Size: 12 58 1442
Fragments arranged by size:
1442 58 12
EcoICRI GAG'CTC
Cuts at: 0 684 762 1512
Size: 684 78 750
Fragments arranged by size:
750 684 78
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 294 830 935 1040 1302 1385 1512
Size: 294 536 105 105 262 83 127
Fragments arranged by size:
536 294 262 127 105 105 83
EcoO109I rG'GnC_Cy
Cuts at: 0 805 1104 1512
Size: 805 299 408
Fragments arranged by size:
805 408 299
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 486 640 1437 1512
Size: 486 154 797 75
Fragments arranged by size:
797 486 154 75
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 32 64 981 1013 1231 1263 1512
Size: 32 32 917 32 218 32 249
Fragments arranged by size:
917 249 218 32 32 32 32
MfeI C'AATT_G
Cuts at: 0 1250 1512
Size: 1250 262
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1103 1512
Size: 1103 409
MslI CAynn'nnrTG
Cuts at: 0 52 111 160 355 665 1512
Size: 52 59 49 195 310 847
Fragments arranged by size:
847 310 195 59 52 49
MspA1I CmG'CkG
Cuts at: 0 979 1512
Size: 979 533
NcoI C'CATG_G
Cuts at: 0 53 1359 1512
Size: 53 1306 153
Fragments arranged by size:
1306 153 53
PflMI CCAn_nnn'nTGG
Cuts at: 0 229 1512
Size: 229 1283
PfoI T'CCnGG_A
Cuts at: 0 807 842 1512
Size: 807 35 670
Fragments arranged by size:
807 670 35
PpuMI rG'GwC_Cy
Cuts at: 0 805 1104 1512
Size: 805 299 408
Fragments arranged by size:
805 408 299
PshAI GACnn'nnGTC
Cuts at: 0 342 1512
Size: 342 1170
PstI C_TGCA'G
Cuts at: 0 1158 1445 1512
Size: 1158 287 67
Fragments arranged by size:
1158 287 67
PvuII CAG'CTG
Cuts at: 0 979 1512
Size: 979 533
SacI G_AGCT'C
Cuts at: 0 686 764 1512
Size: 686 78 748
Fragments arranged by size:
748 686 78
ScaI AGT'ACT
Cuts at: 0 610 1512
Size: 610 902
SfcI C'TryA_G
Cuts at: 0 1154 1441 1512
Size: 1154 287 71
Fragments arranged by size:
1154 287 71
SmlI C'TyrA_G
Cuts at: 0 10 298 1512
Size: 10 288 1214
Fragments arranged by size:
1214 288 10
StuI AGG'CCT
Cuts at: 0 8 1512
Size: 8 1504
StyI C'CwwG_G
Cuts at: 0 53 705 1107 1359 1512
Size: 53 652 402 252 153
Fragments arranged by size:
652 402 252 153 53
TatI w'GTAC_w
Cuts at: 0 608 1512
Size: 608 904
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 359 391 720 752 824 856 1052 1084
Size: 359 32 329 32 72 32 196 32
Cuts at: 1084 1512
Size: 428
Fragments arranged by size:
428 359 329 196 72 32 32 32
32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 361 1512
Size: 361 1151
XmnI GAAnn'nnTTC
Cuts at: 0 923 1512
Size: 923 589
Enzymes that do cut:
AclI AcuI AflII AleI AlwNI ApoI AvaI BaeI
BanII BbsI BcgI BciVI BlpI Bme1580I BplI BpmI
BpuEI BsaI BsaBI BsaXI BseRI BseYI BsgI BsiHKAI
Bsp1286I BspHI BstBI BstEII BstXI BstYI BtgI BtsI
CspCI DraIII DrdI EarI EcoICRI Eco57MI EcoO109I FalI
Hin4I MfeI MmeI MslI MspA1I NcoI PflMI PfoI
PpuMI PshAI PstI PvuII SacI ScaI SfcI SmlI
StuI StyI TatI TstI XcmI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AfeI AflIII AgeI AhdI
AlfI AloI ApaI ApaLI AscI AseI AsiSI AvrII
BamHI BanI BbeI BbvCI BclI BfrBI BglI BglII
BmgBI BmrI BmtI Bpu10I BsaAI BsaHI BsaWI BsiEI
BsiWI BsmI BsmBI BspEI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstZ17I Bsu36I BtgZI ClaI
DraI EaeI EagI EciI EcoNI EcoRI EcoRV FseI
FspI FspAI HaeII HincII HindIII HpaI KasI KpnI
MluI MscI NaeI NarI NdeI NgoMIV NheI NotI
NruI NsiI NspI PacI PasI PciI PmeI PmlI
PpiI PsiI PspOMI PspXI PsrI PvuI RsrII SacII
SalI SanDI SapI SbfI SexAI SfiI SfoI SgfI
SgrAI SmaI SnaBI SpeI SphI SrfI SspI SwaI
TaqII Tth111I XbaI XhoI XmaI ZraI