(Linear) (Six-base) MAPSORT of: KIEE3216.seq  Check: 1278  from: 1  to: 1479



KIEE3216.orf

 With 182 enzymes: SgfI * 

                        March 22, 2012 16:22  ..

Acc65I G'GTAC_C

Cuts at:      0    1191    1479 
   Size:       1191     288
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     665     929    1479 
   Size:        665     264     550

  Fragments arranged by size: 

                 665     550     264
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1363    1479 
   Size:       1363     116
 
AlwNI CAG_nnn'CTG

Cuts at:      0     391     640    1479 
   Size:        391     249     839

  Fragments arranged by size: 

                 839     391     249
 
ApaLI G'TGCA_C

Cuts at:      0    1375    1479 
   Size:       1375     104
 
AvaI C'yCGr_G

Cuts at:      0      52    1479 
   Size:         52    1427
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0    1376    1409    1479 
   Size:       1376      33      70

  Fragments arranged by size: 

                1376      70      33
 
BanI G'GyrC_C

Cuts at:      0     624    1191    1479 
   Size:        624     567     288

  Fragments arranged by size: 

                 624     567     288
 
BclI T'GATC_A

Cuts at:      0     564     819     963    1479 
   Size:        564     255     144     516

  Fragments arranged by size: 

                 564     516     255     144
 
BglII A'GATC_T

Cuts at:      0     168    1479 
   Size:        168    1311
 
BlpI GC'TnA_GC

Cuts at:      0     518    1479 
   Size:        518     961
 
Bme1580I G_kGCm'C

Cuts at:      0    1379    1479 
   Size:       1379     100
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     354    1301    1479 
   Size:        354     947     178

  Fragments arranged by size: 

                 947     354     178
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0      91    1479 
   Size:         91    1388
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1409    1479 
   Size:       1409      70
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      25    1479 
   Size:         25    1454
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     656    1120    1315    1479 
   Size:        656     464     195     164

  Fragments arranged by size: 

                 656     464     195     164
 
BseYI C'CCAG_C

Cuts at:      0     504     513    1479 
   Size:        504       9     966

  Fragments arranged by size: 

                 966     504       9
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     718    1181    1234    1424    1479 
   Size:        718     463      53     190      55

  Fragments arranged by size: 

                 718     463     190      55      53
 
BsiHKAI G_wGCw'C

Cuts at:      0    1352    1379    1479 
   Size:       1352      27     100

  Fragments arranged by size: 

                1352     100      27
 
BsmI GAATG_Cn'

Cuts at:      0    1116    1479 
   Size:       1116     363
 
Bsp1286I G_dGCh'C

Cuts at:      0    1352    1379    1479 
   Size:       1352      27     100

  Fragments arranged by size: 

                1352     100      27
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     690    1203    1479 
   Size:        690     513     276

  Fragments arranged by size: 

                 690     513     276
 
BssSI C'ACGA_G

Cuts at:      0    1019    1479 
   Size:       1019     460
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1219    1479 
   Size:       1219     260
 
BstYI r'GATC_y

Cuts at:      0     168    1479 
   Size:        168    1311
 
Bsu36I CC'TnA_GG

Cuts at:      0     830    1187    1479 
   Size:        830     357     292

  Fragments arranged by size: 

                 830     357     292
 
BtsI GCAGTG_nn'

Cuts at:      0    1379    1479 
   Size:       1379     100
 
DraI TTT'AAA

Cuts at:      0     248    1479 
   Size:        248    1231
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     665     929    1409    1479 
   Size:        665     264     480      70

  Fragments arranged by size: 

                 665     480     264      70
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     193     225     847     879    1479 
   Size:        193      32     622      32     600

  Fragments arranged by size: 

                 622     600     193      32      32
 
HincII GTy'rAC

Cuts at:      0     866    1479 
   Size:        866     613
 
KpnI G_GTAC'C

Cuts at:      0    1195    1479 
   Size:       1195     284
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     496    1082    1479 
   Size:        496     586     397

  Fragments arranged by size: 

                 586     496     397
 
MspA1I CmG'CkG

Cuts at:      0     478     508    1474    1479 
   Size:        478      30     966       5

  Fragments arranged by size: 

                 966     478      30       5
 
NspI r_CATG'y

Cuts at:      0    1065    1479 
   Size:       1065     414
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1332    1479 
   Size:       1332     147
 
PfoI T'CCnGG_A

Cuts at:      0    1205    1479 
   Size:       1205     274
 
PspXI vC'TCGA_Gb

Cuts at:      0      52    1479 
   Size:         52    1427
 
PstI C_TGCA'G

Cuts at:      0    1368    1479 
   Size:       1368     111
 
PvuII CAG'CTG

Cuts at:      0     478     508    1474    1479 
   Size:        478      30     966       5

  Fragments arranged by size: 

                 966     478      30       5
 
SfcI C'TryA_G

Cuts at:      0     269     305    1037    1076    1364    1479 
   Size:        269      36     732      39     288     115

  Fragments arranged by size: 

                 732     288     269     115      39      36
 
SmlI C'TyrA_G

Cuts at:      0      40      52    1479 
   Size:         40      12    1427

  Fragments arranged by size: 

                1427      40      12
 
SspI AAT'ATT

Cuts at:      0    1120    1479 
   Size:       1120     359
 
StuI AGG'CCT

Cuts at:      0    1433    1479 
   Size:       1433      46
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     373    1479 
   Size:        373    1106
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     348     380    1479 
   Size:        348      32    1099

  Fragments arranged by size: 

                1099     348      32
 
XhoI C'TCGA_G

Cuts at:      0      52    1479 
   Size:         52    1427
 

 Enzymes that do cut:

   Acc65I     AcuI     AlfI    AlwNI    ApaLI     AvaI     BaeI     BanI
     BclI    BglII     BlpI Bme1580I     BmrI     BplI     BpmI    BpuEI
    BseRI    BseYI     BsgI  BsiHKAI     BsmI Bsp1286I    BspMI    BssSI
   BstAPI    BstYI   Bsu36I     BtsI     DraI  Eco57MI    Hin4I   HincII
     KpnI     MmeI   MspA1I     NspI    PflMI     PfoI    PspXI     PstI
    PvuII     SfcI     SmlI     SspI     StuI    TaqII     TstI     XhoI

 Enzymes that do not cut: 

     AarI    AatII     AccI     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AloI     ApaI     ApoI     AscI     AseI    AsiSI
    AvrII    BamHI    BanII     BbeI     BbsI    BbvCI     BcgI    BciVI
    BfrBI     BglI    BmgBI     BmtI   Bpu10I     BsaI    BsaAI    BsaBI
    BsaHI    BsaWI    BsaXI    BsiEI    BsiWI    BsmBI    BspEI    BspHI
    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BstBI   BstEII    BstXI
  BstZ17I     BtgI    BtgZI     ClaI    CspCI   DraIII     DrdI     EaeI
     EagI     EarI     EciI  EcoICRI    EcoNI EcoO109I    EcoRI    EcoRV
     FalI     FseI     FspI    FspAI    HaeII  HindIII     HpaI     KasI
     MfeI     MluI     MscI     MslI     NaeI     NarI     NcoI     NdeI
   NgoMIV     NheI     NotI     NruI     NsiI     PacI     PasI     PciI
     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI     PsrI
     PvuI    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI
     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI
     SpeI     SphI     SrfI     StyI     SwaI     TatI  Tth111I     XbaI
     XcmI     XmaI     XmnI     ZraI