Restriction map for
pFN21AE3215
(Linear) (Six-base) MAPSORT of: KIEE3215.seq Check: 1518 from: 1 to: 1473
KIEE3215.orf
With 182 enzymes: SgfI *
July 3, 2012 17:27 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 951 1473
Size: 951 522
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1319 1473
Size: 1319 154
AflIII A'CryG_T
Cuts at: 0 738 1473
Size: 738 735
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1037 1473
Size: 1037 436
AlwNI CAG_nnn'CTG
Cuts at: 0 943 1473
Size: 943 530
ApoI r'AATT_y
Cuts at: 0 74 903 1473
Size: 74 829 570
Fragments arranged by size:
829 570 74
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 299 332 1473
Size: 299 33 1141
Fragments arranged by size:
1141 299 33
BamHI G'GATC_C
Cuts at: 0 43 1473
Size: 43 1430
BanII G_rGCy'C
Cuts at: 0 1321 1473
Size: 1321 152
BmrI ACTGGGnnnn_n'
Cuts at: 0 1284 1473
Size: 1284 189
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 502 1473
Size: 502 971
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 773 1193 1473
Size: 773 420 280
Fragments arranged by size:
773 420 280
Bpu10I CC'TnA_GC
Cuts at: 0 650 1473
Size: 650 823
BsaBI GATnn'nnATC
Cuts at: 0 286 1473
Size: 286 1187
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1122 1152 1473
Size: 1122 30 321
Fragments arranged by size:
1122 321 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 776 1028 1082 1196 1250 1473
Size: 776 252 54 114 54 223
Fragments arranged by size:
776 252 223 114 54 54
BseYI C'CCAG_C
Cuts at: 0 936 1473
Size: 936 537
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 104 475 1235 1349 1473
Size: 104 371 760 114 124
Fragments arranged by size:
760 371 124 114 104
BsiEI CG_ry'CG
Cuts at: 0 826 1473
Size: 826 647
BsiHKAI G_wGCw'C
Cuts at: 0 755 786 1473
Size: 755 31 687
Fragments arranged by size:
755 687 31
Bsp1286I G_dGCh'C
Cuts at: 0 755 786 1321 1473
Size: 755 31 535 152
Fragments arranged by size:
755 535 152 31
BspHI T'CATG_A
Cuts at: 0 713 1473
Size: 713 760
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 126 951 1075 1473
Size: 126 825 124 398
Fragments arranged by size:
825 398 126 124
BsrGI T'GTAC_A
Cuts at: 0 472 1473
Size: 472 1001
BssSI C'ACGA_G
Cuts at: 0 476 1473
Size: 476 997
BstAPI GCAn_nnn'nTGC
Cuts at: 0 741 1473
Size: 741 732
BstYI r'GATC_y
Cuts at: 0 43 1473
Size: 43 1430
BtgI C'CryG_G
Cuts at: 0 803 1473
Size: 803 670
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 547 1473
Size: 547 926
BtsI GCAGTG_nn'
Cuts at: 0 475 1473
Size: 475 998
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 713 748 1473
Size: 713 35 725
Fragments arranged by size:
725 713 35
DraIII CAC_nnn'GTG
Cuts at: 0 172 1473
Size: 172 1301
EarI CTCTTCn'nnn_
Cuts at: 0 493 1473
Size: 493 980
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 686 1394 1473
Size: 686 708 79
Fragments arranged by size:
708 686 79
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 773 1193 1319 1473
Size: 773 420 126 154
Fragments arranged by size:
773 420 154 126
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1208 1473
Size: 1208 265
EcoO109I rG'GnC_Cy
Cuts at: 0 1213 1473
Size: 1213 260
EcoRI G'AATT_C
Cuts at: 0 74 1473
Size: 74 1399
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1122 1154 1473
Size: 1122 32 319
Fragments arranged by size:
1122 319 32
HincII GTy'rAC
Cuts at: 0 973 1473
Size: 973 500
MslI CAynn'nnrTG
Cuts at: 0 323 715 1473
Size: 323 392 758
Fragments arranged by size:
758 392 323
NcoI C'CATG_G
Cuts at: 0 803 1473
Size: 803 670
NdeI CA'TA_TG
Cuts at: 0 569 683 1473
Size: 569 114 790
Fragments arranged by size:
790 569 114
NspI r_CATG'y
Cuts at: 0 382 742 1473
Size: 382 360 731
Fragments arranged by size:
731 382 360
PciI A'CATG_T
Cuts at: 0 738 1473
Size: 738 735
PflMI CCAn_nnn'nTGG
Cuts at: 0 1426 1473
Size: 1426 47
PfoI T'CCnGG_A
Cuts at: 0 255 1209 1425 1473
Size: 255 954 216 48
Fragments arranged by size:
954 255 216 48
PpuMI rG'GwC_Cy
Cuts at: 0 1213 1473
Size: 1213 260
PsiI TTA'TAA
Cuts at: 0 194 661 1271 1473
Size: 194 467 610 202
Fragments arranged by size:
610 467 202 194
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 465 497 1473
Size: 465 32 976
Fragments arranged by size:
976 465 32
PstI C_TGCA'G
Cuts at: 0 1086 1473
Size: 1086 387
PvuI CG_AT'CG
Cuts at: 0 826 1473
Size: 826 647
SexAI A'CCwGG_T
Cuts at: 0 168 1473
Size: 168 1305
SfcI C'TryA_G
Cuts at: 0 441 1082 1473
Size: 441 641 391
Fragments arranged by size:
641 441 391
StyI C'CwwG_G
Cuts at: 0 47 803 1473
Size: 47 756 670
Fragments arranged by size:
756 670 47
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 40 816 1473
Size: 40 776 657
Fragments arranged by size:
776 657 40
TatI w'GTAC_w
Cuts at: 0 472 1473
Size: 472 1001
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 574 1473
Size: 574 899
XmnI GAAnn'nnTTC
Cuts at: 0 611 842 1473
Size: 611 231 631
Fragments arranged by size:
631 611 231
Enzymes that do cut:
AarI AcuI AflIII AlfI AlwNI ApoI BaeI BamHI
BanII BmrI BplI BpmI Bpu10I BsaBI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI Bsp1286I BspHI BspMI BsrGI
BssSI BstAPI BstYI BtgI BtgZI BtsI CspCI DraIII
EarI EciI Eco57MI EcoNI EcoO109I EcoRI Hin4I HincII
MslI NcoI NdeI NspI PciI PflMI PfoI PpuMI
PsiI PsrI PstI PvuI SexAI SfcI StyI TaqII
TatI XcmI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AgeI AhdI
AleI AloI ApaI ApaLI AscI AseI AsiSI AvaI
AvrII BanI BbeI BbsI BbvCI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmtI BpuEI
BsaI BsaAI BsaHI BsaWI BsiWI BsmI BsmBI BspEI
BsrBI BsrDI BsrFI BssHII BstBI BstEII BstXI BstZ17I
Bsu36I ClaI DraI DrdI EaeI EagI EcoICRI EcoRV
FalI FseI FspI FspAI HaeII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MspA1I NaeI NarI
NgoMIV NheI NotI NruI NsiI PacI PasI PmeI
PmlI PpiI PshAI PspOMI PspXI PvuII RsrII SacI
SacII SalI SanDI SapI SbfI ScaI SfiI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI
SspI StuI SwaI TstI Tth111I XbaI XhoI XmaI
ZraI