Restriction map for
pFN21AE3202
(Linear) (Six-base) MAPSORT of: KIEE3202.seq Check: 5006 from: 1 to: 1413
KIEE3202.orf
With 182 enzymes: SgfI *
July 3, 2012 17:25 ..
AccI GT'mk_AC
Cuts at: 0 722 1413
Size: 722 691
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 358 916 1061 1413
Size: 358 558 145 352
Fragments arranged by size:
558 358 352 145
AleI CACnn'nnGTG
Cuts at: 0 130 1279 1413
Size: 130 1149 134
Fragments arranged by size:
1149 134 130
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 94 1413
Size: 94 1319
AlwNI CAG_nnn'CTG
Cuts at: 0 1122 1413
Size: 1122 291
ApoI r'AATT_y
Cuts at: 0 274 1045 1413
Size: 274 771 368
Fragments arranged by size:
771 368 274
AvaI C'yCGr_G
Cuts at: 0 155 1370 1413
Size: 155 1215 43
Fragments arranged by size:
1215 155 43
BanI G'GyrC_C
Cuts at: 0 1193 1413
Size: 1193 220
BanII G_rGCy'C
Cuts at: 0 23 97 1413
Size: 23 74 1316
Fragments arranged by size:
1316 74 23
BbsI GAAGACnn'nnnn_
Cuts at: 0 578 1413
Size: 578 835
BbvCI CC'TCA_GC
Cuts at: 0 938 1413
Size: 938 475
BciVI GTATCCnnnnn_n'
Cuts at: 0 1386 1413
Size: 1386 27
BclI T'GATC_A
Cuts at: 0 134 1413
Size: 134 1279
BglI GCCn_nnn'nGGC
Cuts at: 0 776 1413
Size: 776 637
BglII A'GATC_T
Cuts at: 0 44 656 1413
Size: 44 612 757
Fragments arranged by size:
757 612 44
Bme1580I G_kGCm'C
Cuts at: 0 673 1252 1413
Size: 673 579 161
Fragments arranged by size:
673 579 161
BmgBI CAC'GTC
Cuts at: 0 666 1413
Size: 666 747
BmrI ACTGGGnnnn_n'
Cuts at: 0 102 1413
Size: 102 1311
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1144 1413
Size: 1144 269
Bpu10I CC'TnA_GC
Cuts at: 0 938 1413
Size: 938 475
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1228 1413
Size: 1228 185
BsaI GGTCTCn'nnnn_
Cuts at: 0 1052 1413
Size: 1052 361
BsaBI GATnn'nnATC
Cuts at: 0 1070 1413
Size: 1070 343
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1018 1413
Size: 1018 395
BseYI C'CCAG_C
Cuts at: 0 1401 1413
Size: 1401 12
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 715 1240 1413
Size: 715 525 173
Fragments arranged by size:
715 525 173
BsiHKAI G_wGCw'C
Cuts at: 0 23 1268 1413
Size: 23 1245 145
Fragments arranged by size:
1245 145 23
Bsp1286I G_dGCh'C
Cuts at: 0 23 97 673 1252 1268 1413
Size: 23 74 576 579 16 145
Fragments arranged by size:
579 576 145 74 23 16
BspHI T'CATG_A
Cuts at: 0 731 1413
Size: 731 682
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1263 1413
Size: 1263 150
BstYI r'GATC_y
Cuts at: 0 44 656 1107 1413
Size: 44 612 451 306
Fragments arranged by size:
612 451 306 44
Bsu36I CC'TnA_GG
Cuts at: 0 1379 1413
Size: 1379 34
BtsI GCAGTG_nn'
Cuts at: 0 260 325 970 1051 1413
Size: 260 65 645 81 362
Fragments arranged by size:
645 362 260 81 65
DraIII CAC_nnn'GTG
Cuts at: 0 1263 1413
Size: 1263 150
EaeI y'GGCC_r
Cuts at: 0 860 1413
Size: 860 553
EarI CTCTTCn'nnn_
Cuts at: 0 913 1413
Size: 913 500
EcoICRI GAG'CTC
Cuts at: 0 21 1413
Size: 21 1392
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 358 916 1061 1144 1413
Size: 358 558 145 83 269
Fragments arranged by size:
558 358 269 145 83
EcoO109I rG'GnC_Cy
Cuts at: 0 942 972 1413
Size: 942 30 441
Fragments arranged by size:
942 441 30
EcoRI G'AATT_C
Cuts at: 0 274 1413
Size: 274 1139
EcoRV GAT'ATC
Cuts at: 0 193 1413
Size: 193 1220
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 541 1413
Size: 541 872
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 791 823 1413
Size: 791 32 590
Fragments arranged by size:
791 590 32
HincII GTy'rAC
Cuts at: 0 606 1413
Size: 606 807
MscI TGG'CCA
Cuts at: 0 862 1413
Size: 862 551
MslI CAynn'nnrTG
Cuts at: 0 130 1279 1413
Size: 130 1149 134
Fragments arranged by size:
1149 134 130
MspA1I CmG'CkG
Cuts at: 0 65 937 1413
Size: 65 872 476
Fragments arranged by size:
872 476 65
PasI CC'CwG_GG
Cuts at: 0 879 945 1413
Size: 879 66 468
Fragments arranged by size:
879 468 66
PflMI CCAn_nnn'nTGG
Cuts at: 0 618 1413
Size: 618 795
PfoI T'CCnGG_A
Cuts at: 0 1025 1211 1413
Size: 1025 186 202
Fragments arranged by size:
1025 202 186
PspXI vC'TCGA_Gb
Cuts at: 0 155 1413
Size: 155 1258
PstI C_TGCA'G
Cuts at: 0 434 1413
Size: 434 979
PvuII CAG'CTG
Cuts at: 0 937 1413
Size: 937 476
SacI G_AGCT'C
Cuts at: 0 23 1413
Size: 23 1390
SexAI A'CCwGG_T
Cuts at: 0 31 1413
Size: 31 1382
SfcI C'TryA_G
Cuts at: 0 430 1413
Size: 430 983
SmaI CCC'GGG
Cuts at: 0 1372 1413
Size: 1372 41
SmlI C'TyrA_G
Cuts at: 0 155 1243 1413
Size: 155 1088 170
Fragments arranged by size:
1088 170 155
StyI C'CwwG_G
Cuts at: 0 617 1088 1413
Size: 617 471 325
Fragments arranged by size:
617 471 325
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 40 937 1413
Size: 40 897 476
Fragments arranged by size:
897 476 40
TatI w'GTAC_w
Cuts at: 0 1161 1413
Size: 1161 252
XhoI C'TCGA_G
Cuts at: 0 155 1413
Size: 155 1258
XmaI C'CCGG_G
Cuts at: 0 1370 1413
Size: 1370 43
XmnI GAAnn'nnTTC
Cuts at: 0 278 563 575 1413
Size: 278 285 12 838
Fragments arranged by size:
838 285 278 12
Enzymes that do cut:
AccI AcuI AleI AlfI AlwNI ApoI AvaI BanI
BanII BbsI BbvCI BciVI BclI BglI BglII Bme1580I
BmgBI BmrI BpmI Bpu10I BpuEI BsaI BsaBI BseRI
BseYI BsgI BsiHKAI Bsp1286I BspHI BstAPI BstYI Bsu36I
BtsI DraIII EaeI EarI EcoICRI Eco57MI EcoO109I EcoRI
EcoRV FalI Hin4I HincII MscI MslI MspA1I PasI
PflMI PfoI PspXI PstI PvuII SacI SexAI SfcI
SmaI SmlI StyI TaqII TatI XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AhdI AloI ApaI ApaLI AscI AseI AsiSI AvrII
BaeI BamHI BbeI BcgI BfrBI BlpI BmtI BplI
BsaAI BsaHI BsaWI BsaXI BsiEI BsiWI BsmI BsmBI
BspEI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstBI BstEII BstXI BstZ17I BtgI BtgZI ClaI CspCI
DraI DrdI EagI EciI EcoNI FseI FspI FspAI
HaeII HindIII HpaI KasI KpnI MfeI MluI MmeI
NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI
NsiI NspI PacI PciI PmeI PmlI PpiI PpuMI
PshAI PsiI PspOMI PsrI PvuI RsrII SacII SalI
SanDI SapI SbfI ScaI SfiI SfoI SgfI SgrAI
SnaBI SpeI SphI SrfI SspI StuI SwaI TstI
Tth111I XbaI XcmI ZraI