Restriction map for
pFN21AE3198
(Linear) (Six-base) MAPSORT of: KIEE3198.seq Check: 9519 from: 1 to: 495
KIEE3198.orf
With 182 enzymes: SgfI *
December 5, 2014 12:56 ..
AatII G_ACGT'C
Cuts at: 0 428 495
Size: 428 67
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 378 495
Size: 378 117
AlwNI CAG_nnn'CTG
Cuts at: 0 291 367 495
Size: 291 76 128
Fragments arranged by size:
291 128 76
ApaI G_GGCC'C
Cuts at: 0 215 495
Size: 215 280
BamHI G'GATC_C
Cuts at: 0 326 495
Size: 326 169
BanI G'GyrC_C
Cuts at: 0 8 185 495
Size: 8 177 310
Fragments arranged by size:
310 177 8
BanII G_rGCy'C
Cuts at: 0 30 215 316 352 495
Size: 30 185 101 36 143
Fragments arranged by size:
185 143 101 36 30
BbeI G_GCGC'C
Cuts at: 0 12 189 495
Size: 12 177 306
Fragments arranged by size:
306 177 12
BglI GCCn_nnn'nGGC
Cuts at: 0 256 495
Size: 256 239
Bme1580I G_kGCm'C
Cuts at: 0 215 495
Size: 215 280
BsaAI yAC'GTr
Cuts at: 0 108 495
Size: 108 387
BsaHI Gr'CG_yC
Cuts at: 0 9 186 425 495
Size: 9 177 239 70
Fragments arranged by size:
239 177 70 9
BsaWI w'CCGG_w
Cuts at: 0 378 495
Size: 378 117
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 217 247 495
Size: 217 30 248
Fragments arranged by size:
248 217 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 251 495
Size: 251 244
BseYI C'CCAG_C
Cuts at: 0 435 495
Size: 435 60
BsiEI CG_ry'CG
Cuts at: 0 43 206 495
Size: 43 163 289
Fragments arranged by size:
289 163 43
BsiHKAI G_wGCw'C
Cuts at: 0 316 495
Size: 316 179
BsmBI CGTCTCn'nnnn_
Cuts at: 0 292 416 432 495
Size: 292 124 16 63
Fragments arranged by size:
292 124 63 16
Bsp1286I G_dGCh'C
Cuts at: 0 30 215 316 352 495
Size: 30 185 101 36 143
Fragments arranged by size:
185 143 101 36 30
BsrBI CCG'CTC
Cuts at: 0 21 495
Size: 21 474
BsrFI r'CCGG_y
Cuts at: 0 38 495
Size: 38 457
BssHII G'CGCG_C
Cuts at: 0 191 198 354 495
Size: 191 7 156 141
Fragments arranged by size:
191 156 141 7
BssSI C'ACGA_G
Cuts at: 0 276 495
Size: 276 219
BstYI r'GATC_y
Cuts at: 0 326 495
Size: 326 169
BtgI C'CryG_G
Cuts at: 0 43 495
Size: 43 452
BtsI GCAGTG_nn'
Cuts at: 0 52 495
Size: 52 443
DraIII CAC_nnn'GTG
Cuts at: 0 132 495
Size: 132 363
EaeI y'GGCC_r
Cuts at: 0 40 203 218 405 495
Size: 40 163 15 187 90
Fragments arranged by size:
187 163 90 40 15
EagI C'GGCC_G
Cuts at: 0 40 203 495
Size: 40 163 292
Fragments arranged by size:
292 163 40
EcoICRI GAG'CTC
Cuts at: 0 314 495
Size: 314 181
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 378 495
Size: 378 117
EcoO109I rG'GnC_Cy
Cuts at: 0 212 495
Size: 212 283
HaeII r_GCGC'y
Cuts at: 0 12 189 495
Size: 12 177 306
Fragments arranged by size:
306 177 12
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 217 249 495
Size: 217 32 246
Fragments arranged by size:
246 217 32
KasI G'GCGC_C
Cuts at: 0 8 185 495
Size: 8 177 310
Fragments arranged by size:
310 177 8
MscI TGG'CCA
Cuts at: 0 220 407 495
Size: 220 187 88
Fragments arranged by size:
220 187 88
MspA1I CmG'CkG
Cuts at: 0 45 81 145 398 439 495
Size: 45 36 64 253 41 56
Fragments arranged by size:
253 64 56 45 41 36
NaeI GCC'GGC
Cuts at: 0 40 495
Size: 40 455
NarI GG'CG_CC
Cuts at: 0 9 186 495
Size: 9 177 309
Fragments arranged by size:
309 177 9
NgoMIV G'CCGG_C
Cuts at: 0 38 495
Size: 38 457
NotI GC'GGCC_GC
Cuts at: 0 203 495
Size: 203 292
PasI CC'CwG_GG
Cuts at: 0 13 495
Size: 13 482
PflMI CCAn_nnn'nTGG
Cuts at: 0 132 495
Size: 132 363
PmlI CAC'GTG
Cuts at: 0 108 495
Size: 108 387
PspOMI G'GGCC_C
Cuts at: 0 211 495
Size: 211 284
RsrII CG'GwC_CG
Cuts at: 0 487 495
Size: 487 8
SacI G_AGCT'C
Cuts at: 0 316 495
Size: 316 179
SacII CC_GC'GG
Cuts at: 0 46 495
Size: 46 449
SfoI GGC'GCC
Cuts at: 0 10 187 495
Size: 10 177 308
Fragments arranged by size:
308 177 10
StyI C'CwwG_G
Cuts at: 0 462 495
Size: 462 33
ZraI GAC'GTC
Cuts at: 0 426 495
Size: 426 69
Enzymes that do cut:
AatII AcuI AlwNI ApaI BamHI BanI BanII BbeI
BglI Bme1580I BsaAI BsaHI BsaWI BsaXI BseRI BseYI
BsiEI BsiHKAI BsmBI Bsp1286I BsrBI BsrFI BssHII BssSI
BstYI BtgI BtsI DraIII EaeI EagI EcoICRI Eco57MI
EcoO109I HaeII Hin4I KasI MscI MspA1I NaeI NarI
NgoMIV NotI PasI PflMI PmlI PspOMI RsrII SacI
SacII SfoI StyI ZraI
Enzymes that do not cut:
AarI AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI ApaLI ApoI AscI AseI
AsiSI AvaI AvrII BaeI BbsI BbvCI BcgI BciVI
BclI BfrBI BglII BlpI BmgBI BmrI BmtI BplI
BpmI Bpu10I BpuEI BsaI BsaBI BsgI BsiWI BsmI
BspEI BspHI BspMI BsrDI BsrGI BstAPI BstBI BstEII
BstXI BstZ17I Bsu36I BtgZI ClaI CspCI DraI DrdI
EarI EciI EcoNI EcoRI EcoRV FalI FseI FspI
FspAI HincII HindIII HpaI KpnI MfeI MluI MmeI
MslI NcoI NdeI NheI NruI NsiI NspI PacI
PciI PfoI PmeI PpiI PpuMI PshAI PsiI PspXI
PsrI PstI PvuI PvuII SalI SanDI SapI SbfI
ScaI SexAI SfcI SfiI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SrfI SspI StuI SwaI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI