Restriction map for
pFN21AE3185
(Linear) (Six-base) MAPSORT of: KIEE3185.seq Check: 5649 from: 1 to: 1275
KIEE3185.orf
With 182 enzymes: SgfI *
March 22, 2012 16:19 ..
AccI GT'mk_AC
Cuts at: 0 382 1275
Size: 382 893
Acc65I G'GTAC_C
Cuts at: 0 481 1275
Size: 481 794
AfeI AGC'GCT
Cuts at: 0 862 1275
Size: 862 413
AleI CACnn'nnGTG
Cuts at: 0 238 370 439 1275
Size: 238 132 69 836
Fragments arranged by size:
836 238 132 69
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 847 1275
Size: 847 428
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1243 1275
Size: 1243 32
AlwNI CAG_nnn'CTG
Cuts at: 0 723 1275
Size: 723 552
ApaI G_GGCC'C
Cuts at: 0 364 495 1275
Size: 364 131 780
Fragments arranged by size:
780 364 131
ApaLI G'TGCA_C
Cuts at: 0 1084 1275
Size: 1084 191
ApoI r'AATT_y
Cuts at: 0 40 160 958 1275
Size: 40 120 798 317
Fragments arranged by size:
798 317 120 40
AvaI C'yCGr_G
Cuts at: 0 344 1275
Size: 344 931
BanI G'GyrC_C
Cuts at: 0 178 481 990 1275
Size: 178 303 509 285
Fragments arranged by size:
509 303 285 178
BanII G_rGCy'C
Cuts at: 0 364 495 709 1275
Size: 364 131 214 566
Fragments arranged by size:
566 364 214 131
BbvCI CC'TCA_GC
Cuts at: 0 738 1275
Size: 738 537
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 493 527 1275
Size: 493 34 748
Fragments arranged by size:
748 493 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 910 1275
Size: 910 365
BglI GCCn_nnn'nGGC
Cuts at: 0 187 1275
Size: 187 1088
BglII A'GATC_T
Cuts at: 0 128 1275
Size: 128 1147
Bme1580I G_kGCm'C
Cuts at: 0 364 495 993 1088 1275
Size: 364 131 498 95 187
Fragments arranged by size:
498 364 187 131 95
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 549 1275
Size: 549 726
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1193 1201 1275
Size: 1193 8 74
Fragments arranged by size:
1193 74 8
Bpu10I CC'TnA_GC
Cuts at: 0 738 1275
Size: 738 537
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1016 1033 1275
Size: 1016 17 242
Fragments arranged by size:
1016 242 17
BsaWI w'CCGG_w
Cuts at: 0 1256 1275
Size: 1256 19
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 791 821 1117 1147 1275
Size: 791 30 296 30 128
Fragments arranged by size:
791 296 128 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1138 1275
Size: 1138 137
BseYI C'CCAG_C
Cuts at: 0 695 1275
Size: 695 580
BsiEI CG_ry'CG
Cuts at: 0 231 1275
Size: 231 1044
BsiHKAI G_wGCw'C
Cuts at: 0 1088 1275
Size: 1088 187
Bsp1286I G_dGCh'C
Cuts at: 0 364 495 709 993 1088 1275
Size: 364 131 214 284 95 187
Fragments arranged by size:
364 284 214 187 131 95
BsrBI CCG'CTC
Cuts at: 0 227 507 1275
Size: 227 280 768
Fragments arranged by size:
768 280 227
BsrGI T'GTAC_A
Cuts at: 0 785 1275
Size: 785 490
BstEII G'GTnAC_C
Cuts at: 0 712 1164 1275
Size: 712 452 111
Fragments arranged by size:
712 452 111
BstXI CCAn_nnnn'nTGG
Cuts at: 0 372 441 1275
Size: 372 69 834
Fragments arranged by size:
834 372 69
BstYI r'GATC_y
Cuts at: 0 128 1266 1275
Size: 128 1138 9
Fragments arranged by size:
1138 128 9
BtgI C'CryG_G
Cuts at: 0 485 1275
Size: 485 790
BtsI GCAGTG_nn'
Cuts at: 0 419 1106 1222 1275
Size: 419 687 116 53
Fragments arranged by size:
687 419 116 53
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 24 59 1275
Size: 24 35 1216
Fragments arranged by size:
1216 35 24
EaeI y'GGCC_r
Cuts at: 0 228 1275
Size: 228 1047
EagI C'GGCC_G
Cuts at: 0 228 1275
Size: 228 1047
EarI CTCTTCn'nnn_
Cuts at: 0 2 326 518 1275
Size: 2 324 192 757
Fragments arranged by size:
757 324 192 2
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1193 1201 1275
Size: 1193 8 74
Fragments arranged by size:
1193 74 8
EcoO109I rG'GnC_Cy
Cuts at: 0 340 361 491 492 543 804 1275
Size: 340 21 130 1 51 261 471
Fragments arranged by size:
471 340 261 130 51 21 1
EcoRV GAT'ATC
Cuts at: 0 1254 1275
Size: 1254 21
HaeII r_GCGC'y
Cuts at: 0 864 1275
Size: 864 411
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 597 629 1117 1149 1275
Size: 597 32 488 32 126
Fragments arranged by size:
597 488 126 32 32
HincII GTy'rAC
Cuts at: 0 113 1275
Size: 113 1162
HindIII A'AGCT_T
Cuts at: 0 592 1275
Size: 592 683
KpnI G_GTAC'C
Cuts at: 0 485 1275
Size: 485 790
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 83 1275
Size: 83 1192
MslI CAynn'nnrTG
Cuts at: 0 238 265 370 439 1162 1275
Size: 238 27 105 69 723 113
Fragments arranged by size:
723 238 113 105 69 27
NcoI C'CATG_G
Cuts at: 0 485 1275
Size: 485 790
NotI GC'GGCC_GC
Cuts at: 0 228 1275
Size: 228 1047
NspI r_CATG'y
Cuts at: 0 733 1029 1275
Size: 733 296 246
Fragments arranged by size:
733 296 246
PflMI CCAn_nnn'nTGG
Cuts at: 0 723 1275
Size: 723 552
PpuMI rG'GwC_Cy
Cuts at: 0 340 804 1275
Size: 340 464 471
Fragments arranged by size:
471 464 340
PsiI TTA'TAA
Cuts at: 0 599 1275
Size: 599 676
PspOMI G'GGCC_C
Cuts at: 0 360 491 1275
Size: 360 131 784
Fragments arranged by size:
784 360 131
PstI C_TGCA'G
Cuts at: 0 516 1275
Size: 516 759
SapI GCTCTTCn'nnn_
Cuts at: 0 326 1275
Size: 326 949
SfcI C'TryA_G
Cuts at: 0 383 512 1275
Size: 383 129 763
Fragments arranged by size:
763 383 129
SmlI C'TyrA_G
Cuts at: 0 1031 1048 1275
Size: 1031 17 227
Fragments arranged by size:
1031 227 17
SphI G_CATG'C
Cuts at: 0 733 1275
Size: 733 542
StyI C'CwwG_G
Cuts at: 0 182 254 296 485 866 1275
Size: 182 72 42 189 381 409
Fragments arranged by size:
409 381 189 182 72 42
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1008 1275
Size: 1008 267
TatI w'GTAC_w
Cuts at: 0 785 1275
Size: 785 490
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 983 1015 1275
Size: 983 32 260
Fragments arranged by size:
983 260 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1176 1275
Size: 1176 99
Enzymes that do cut:
AccI Acc65I AfeI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AvaI BanI BanII BbvCI BcgI BciVI
BglI BglII Bme1580I BplI BpmI Bpu10I BpuEI BsaWI
BsaXI BseRI BseYI BsiEI BsiHKAI Bsp1286I BsrBI BsrGI
BstEII BstXI BstYI BtgI BtsI CspCI EaeI EagI
EarI Eco57MI EcoO109I EcoRV HaeII Hin4I HincII HindIII
KpnI MmeI MslI NcoI NotI NspI PflMI PpuMI
PsiI PspOMI PstI SapI SfcI SmlI SphI StyI
TaqII TatI TstI XcmI
Enzymes that do not cut:
AarI AatII AclI AcuI AflII AflIII AgeI AhdI
AscI AseI AsiSI AvrII BaeI BamHI BbeI BbsI
BclI BfrBI BlpI BmgBI BmrI BmtI BsaI BsaAI
BsaBI BsaHI BsgI BsiWI BsmI BsmBI BspEI BspHI
BspMI BsrDI BsrFI BssHII BssSI BstAPI BstBI BstZ17I
Bsu36I BtgZI ClaI DraI DraIII DrdI EciI EcoICRI
EcoNI EcoRI FalI FseI FspI FspAI HpaI KasI
MfeI MluI MscI MspA1I NaeI NarI NdeI NgoMIV
NheI NruI NsiI PacI PasI PciI PfoI PmeI
PmlI PpiI PshAI PspXI PsrI PvuI PvuII RsrII
SacI SacII SalI SanDI SbfI ScaI SexAI SfiI
SfoI SgfI SgrAI SmaI SnaBI SpeI SrfI SspI
StuI SwaI Tth111I XbaI XhoI XmaI XmnI ZraI