Restriction map for
pFN21AE3184
(Linear) (Six-base) MAPSORT of: KIEE3184.seq Check: 8695 from: 1 to: 1266
KIEE3184.orf
With 182 enzymes: SgfI *
March 22, 2012 16:19 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 414 1266
Size: 414 852
AflII C'TTAA_G
Cuts at: 0 721 1266
Size: 721 545
AleI CACnn'nnGTG
Cuts at: 0 349 1266
Size: 349 917
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 545 577 1266
Size: 545 32 689
Fragments arranged by size:
689 545 32
ApaLI G'TGCA_C
Cuts at: 0 1110 1266
Size: 1110 156
AvrII C'CTAG_G
Cuts at: 0 1228 1266
Size: 1228 38
BanII G_rGCy'C
Cuts at: 0 56 898 1144 1266
Size: 56 842 246 122
Fragments arranged by size:
842 246 122 56
BclI T'GATC_A
Cuts at: 0 353 1266
Size: 353 913
Bme1580I G_kGCm'C
Cuts at: 0 864 1087 1114 1266
Size: 864 223 27 152
Fragments arranged by size:
864 223 152 27
BmrI ACTGGGnnnn_n'
Cuts at: 0 469 867 1266
Size: 469 398 399
Fragments arranged by size:
469 399 398
Bpu10I CC'TnA_GC
Cuts at: 0 137 1266
Size: 137 1129
BsaI GGTCTCn'nnnn_
Cuts at: 0 460 771 793 1266
Size: 460 311 22 473
Fragments arranged by size:
473 460 311 22
BsaAI yAC'GTr
Cuts at: 0 1077 1266
Size: 1077 189
BsaBI GATnn'nnATC
Cuts at: 0 633 1266
Size: 633 633
BsaHI Gr'CG_yC
Cuts at: 0 653 1266
Size: 653 613
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 545 575 1266
Size: 545 30 691
Fragments arranged by size:
691 545 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 383 811 1177 1266
Size: 383 428 366 89
Fragments arranged by size:
428 383 366 89
BseYI C'CCAG_C
Cuts at: 0 49 1266
Size: 49 1217
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 937 1057 1266
Size: 937 120 209
Fragments arranged by size:
937 209 120
BsiHKAI G_wGCw'C
Cuts at: 0 1114 1266
Size: 1114 152
Bsp1286I G_dGCh'C
Cuts at: 0 56 864 898 1087 1114 1144 1266
Size: 56 808 34 189 27 30 122
Fragments arranged by size:
808 189 122 56 34 30 27
BsrDI GCAATG_nn'
Cuts at: 0 620 1266
Size: 620 646
BsrFI r'CCGG_y
Cuts at: 0 619 1266
Size: 619 647
BsrGI T'GTAC_A
Cuts at: 0 807 824 1266
Size: 807 17 442
Fragments arranged by size:
807 442 17
BstBI TT'CG_AA
Cuts at: 0 24 1266
Size: 24 1242
BstYI r'GATC_y
Cuts at: 0 634 1266
Size: 634 632
Bsu36I CC'TnA_GG
Cuts at: 0 112 1266
Size: 112 1154
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1189 1266
Size: 1189 77
BtsI GCAGTG_nn'
Cuts at: 0 379 1266
Size: 379 887
DraI TTT'AAA
Cuts at: 0 15 1266
Size: 15 1251
DraIII CAC_nnn'GTG
Cuts at: 0 1091 1266
Size: 1091 175
EarI CTCTTCn'nnn_
Cuts at: 0 1228 1266
Size: 1228 38
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 414 1266
Size: 414 852
EcoO109I rG'GnC_Cy
Cuts at: 0 766 1266
Size: 766 500
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 836 1266
Size: 836 430
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 540 1266
Size: 540 726
MslI CAynn'nnrTG
Cuts at: 0 280 349 1266
Size: 280 69 917
Fragments arranged by size:
917 280 69
MspA1I CmG'CkG
Cuts at: 0 49 921 1266
Size: 49 872 345
Fragments arranged by size:
872 345 49
PfoI T'CCnGG_A
Cuts at: 0 326 1124 1266
Size: 326 798 142
Fragments arranged by size:
798 326 142
PmlI CAC'GTG
Cuts at: 0 1077 1266
Size: 1077 189
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 545 577 1232 1264 1266
Size: 545 32 655 32 2
Fragments arranged by size:
655 545 32 32 2
PpuMI rG'GwC_Cy
Cuts at: 0 766 1266
Size: 766 500
PsiI TTA'TAA
Cuts at: 0 1036 1266
Size: 1036 230
PstI C_TGCA'G
Cuts at: 0 210 386 1214 1266
Size: 210 176 828 52
Fragments arranged by size:
828 210 176 52
PvuII CAG'CTG
Cuts at: 0 49 921 1266
Size: 49 872 345
Fragments arranged by size:
872 345 49
ScaI AGT'ACT
Cuts at: 0 670 1266
Size: 670 596
SfcI C'TryA_G
Cuts at: 0 44 206 382 770 1040 1210 1266
Size: 44 162 176 388 270 170 56
Fragments arranged by size:
388 270 176 170 162 56 44
SmlI C'TyrA_G
Cuts at: 0 721 1266
Size: 721 545
SspI AAT'ATT
Cuts at: 0 836 1266
Size: 836 430
StuI AGG'CCT
Cuts at: 0 816 1266
Size: 816 450
StyI C'CwwG_G
Cuts at: 0 1228 1266
Size: 1228 38
TatI w'GTAC_w
Cuts at: 0 668 807 824 1266
Size: 668 139 17 442
Fragments arranged by size:
668 442 139 17
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 85 324 1074 1266
Size: 85 239 750 192
Fragments arranged by size:
750 239 192 85
XmnI GAAnn'nnTTC
Cuts at: 0 986 1266
Size: 986 280
Enzymes that do cut:
AcuI AflII AleI AloI ApaLI AvrII BanII BclI
Bme1580I BmrI Bpu10I BsaI BsaAI BsaBI BsaHI BsaXI
BseRI BseYI BsgI BsiHKAI Bsp1286I BsrDI BsrFI BsrGI
BstBI BstYI Bsu36I BtgZI BtsI DraI DraIII EarI
Eco57MI EcoO109I FalI MmeI MslI MspA1I PfoI PmlI
PpiI PpuMI PsiI PstI PvuII ScaI SfcI SmlI
SspI StuI StyI TatI XcmI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflIII AgeI
AhdI AlfI AlwNI ApaI ApoI AscI AseI AsiSI
AvaI BaeI BamHI BanI BbeI BbsI BbvCI BcgI
BciVI BfrBI BglI BglII BlpI BmgBI BmtI BplI
BpmI BpuEI BsaWI BsiEI BsiWI BsmI BsmBI BspEI
BspHI BspMI BsrBI BssHII BssSI BstAPI BstEII BstXI
BstZ17I BtgI ClaI CspCI DrdI EaeI EagI EciI
EcoICRI EcoNI EcoRI EcoRV FseI FspI FspAI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MscI NaeI NarI NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspI PacI PasI PciI PflMI PmeI
PshAI PspOMI PspXI PsrI PvuI RsrII SacI SacII
SalI SanDI SapI SbfI SexAI SfiI SfoI SgfI
SgrAI SmaI SnaBI SpeI SphI SrfI SwaI TaqII
TstI Tth111I XbaI XhoI XmaI ZraI