Restriction map for
pFN21AE3183
(Linear) (Six-base) MAPSORT of: KIEE3183.seq Check: 5887 from: 1 to: 1266
KIEE3183.orf
With 182 enzymes: SgfI *
December 27, 2011 16:34 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 154 466 542 671 1266
Size: 154 312 76 129 595
Fragments arranged by size:
595 312 154 129 76
AfeI AGC'GCT
Cuts at: 0 172 891 1266
Size: 172 719 375
Fragments arranged by size:
719 375 172
AflIII A'CryG_T
Cuts at: 0 551 1266
Size: 551 715
AleI CACnn'nnGTG
Cuts at: 0 1129 1266
Size: 1129 137
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 552 1136 1266
Size: 552 584 130
Fragments arranged by size:
584 552 130
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1013 1045 1266
Size: 1013 32 221
Fragments arranged by size:
1013 221 32
AlwNI CAG_nnn'CTG
Cuts at: 0 51 191 444 739 1266
Size: 51 140 253 295 527
Fragments arranged by size:
527 295 253 140 51
ApaI G_GGCC'C
Cuts at: 0 103 116 1266
Size: 103 13 1150
Fragments arranged by size:
1150 103 13
ApaLI G'TGCA_C
Cuts at: 0 537 1266
Size: 537 729
AvaI C'yCGr_G
Cuts at: 0 102 142 1266
Size: 102 40 1124
Fragments arranged by size:
1124 102 40
BamHI G'GATC_C
Cuts at: 0 297 1266
Size: 297 969
BanI G'GyrC_C
Cuts at: 0 69 288 1266
Size: 69 219 978
Fragments arranged by size:
978 219 69
BanII G_rGCy'C
Cuts at: 0 103 116 779 1266
Size: 103 13 663 487
Fragments arranged by size:
663 487 103 13
BbeI G_GCGC'C
Cuts at: 0 73 1266
Size: 73 1193
BbsI GAAGACnn'nnnn_
Cuts at: 0 548 554 602 1265 1266
Size: 548 6 48 663 1
Fragments arranged by size:
663 548 48 6 1
BbvCI CC'TCA_GC
Cuts at: 0 488 635 1266
Size: 488 147 631
Fragments arranged by size:
631 488 147
BclI T'GATC_A
Cuts at: 0 1106 1266
Size: 1106 160
BglI GCCn_nnn'nGGC
Cuts at: 0 21 238 1266
Size: 21 217 1028
Fragments arranged by size:
1028 217 21
BlpI GC'TnA_GC
Cuts at: 0 205 1266
Size: 205 1061
Bme1580I G_kGCm'C
Cuts at: 0 103 116 347 541 1266
Size: 103 13 231 194 725
Fragments arranged by size:
725 231 194 103 13
BmgBI CAC'GTC
Cuts at: 0 552 1266
Size: 552 714
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 892 992 1040 1098 1266
Size: 892 100 48 58 168
Fragments arranged by size:
892 168 100 58 48
Bpu10I CC'TnA_GC
Cuts at: 0 488 635 778 1266
Size: 488 147 143 488
Fragments arranged by size:
488 488 147 143
BsaHI Gr'CG_yC
Cuts at: 0 70 1266
Size: 70 1196
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 254 284 1013 1043 1266
Size: 254 30 729 30 223
Fragments arranged by size:
729 254 223 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 589 859 1266
Size: 589 270 407
Fragments arranged by size:
589 407 270
BseYI C'CCAG_C
Cuts at: 0 51 226 1266
Size: 51 175 1040
Fragments arranged by size:
1040 175 51
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 752 769 1171 1237 1266
Size: 752 17 402 66 29
Fragments arranged by size:
752 402 66 29 17
BsiHKAI G_wGCw'C
Cuts at: 0 541 779 1194 1266
Size: 541 238 415 72
Fragments arranged by size:
541 415 238 72
Bsp1286I G_dGCh'C
Cuts at: 0 103 116 347 541 779 1194 1266
Size: 103 13 231 194 238 415 72
Fragments arranged by size:
415 238 231 194 103 72 13
BsrDI GCAATG_nn'
Cuts at: 0 179 1266
Size: 179 1087
BsrFI r'CCGG_y
Cuts at: 0 761 1266
Size: 761 505
BssSI C'ACGA_G
Cuts at: 0 166 832 1145 1266
Size: 166 666 313 121
Fragments arranged by size:
666 313 166 121
BstAPI GCAn_nnn'nTGC
Cuts at: 0 314 1266
Size: 314 952
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1053 1266
Size: 1053 213
BstYI r'GATC_y
Cuts at: 0 297 911 1220 1266
Size: 297 614 309 46
Fragments arranged by size:
614 309 297 46
Bsu36I CC'TnA_GG
Cuts at: 0 121 584 1266
Size: 121 463 682
Fragments arranged by size:
682 463 121
BtgI C'CryG_G
Cuts at: 0 159 277 1266
Size: 159 118 989
Fragments arranged by size:
989 159 118
BtsI GCAGTG_nn'
Cuts at: 0 312 541 740 1089 1266
Size: 312 229 199 349 177
Fragments arranged by size:
349 312 229 199 177
DraI TTT'AAA
Cuts at: 0 963 1266
Size: 963 303
EaeI y'GGCC_r
Cuts at: 0 162 680 1266
Size: 162 518 586
Fragments arranged by size:
586 518 162
EarI CTCTTCn'nnn_
Cuts at: 0 442 620 1266
Size: 442 178 646
Fragments arranged by size:
646 442 178
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 827 1052 1266
Size: 827 225 214
Fragments arranged by size:
827 225 214
EcoICRI GAG'CTC
Cuts at: 0 777 1266
Size: 777 489
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 154 466 542 671 892 992 1040 1098
Size: 154 312 76 129 221 100 48 58
Cuts at: 1098 1266
Size: 168
Fragments arranged by size:
312 221 168 154 129 100 76 58
48
EcoNI CCTnn'n_nnAGG
Cuts at: 0 120 1266
Size: 120 1146
EcoO109I rG'GnC_Cy
Cuts at: 0 99 112 113 1266
Size: 99 13 1 1153
Fragments arranged by size:
1153 99 13 1
HaeII r_GCGC'y
Cuts at: 0 73 174 191 893 1266
Size: 73 101 17 702 373
Fragments arranged by size:
702 373 101 73 17
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 254 286 498 530 1031 1063 1266
Size: 254 32 212 32 501 32 203
Fragments arranged by size:
501 254 212 203 32 32 32
HincII GTy'rAC
Cuts at: 0 879 1266
Size: 879 387
KasI G'GCGC_C
Cuts at: 0 69 1266
Size: 69 1197
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 485 1266
Size: 485 781
MscI TGG'CCA
Cuts at: 0 682 1266
Size: 682 584
MslI CAynn'nnrTG
Cuts at: 0 497 1051 1129 1266
Size: 497 554 78 137
Fragments arranged by size:
554 497 137 78
MspA1I CmG'CkG
Cuts at: 0 51 161 634 1266
Size: 51 110 473 632
Fragments arranged by size:
632 473 110 51
NarI GG'CG_CC
Cuts at: 0 70 1266
Size: 70 1196
PasI CC'CwG_GG
Cuts at: 0 91 1266
Size: 91 1175
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1013 1045 1266
Size: 1013 32 221
Fragments arranged by size:
1013 221 32
PspOMI G'GGCC_C
Cuts at: 0 99 112 1266
Size: 99 13 1154
Fragments arranged by size:
1154 99 13
PspXI vC'TCGA_Gb
Cuts at: 0 142 1266
Size: 142 1124
PvuII CAG'CTG
Cuts at: 0 51 634 1266
Size: 51 583 632
Fragments arranged by size:
632 583 51
RsrII CG'GwC_CG
Cuts at: 0 262 1266
Size: 262 1004
SacI G_AGCT'C
Cuts at: 0 779 1266
Size: 779 487
SacII CC_GC'GG
Cuts at: 0 162 1266
Size: 162 1104
SapI GCTCTTCn'nnn_
Cuts at: 0 620 1266
Size: 620 646
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 238 1266
Size: 238 1028
SfoI GGC'GCC
Cuts at: 0 71 1266
Size: 71 1195
SmaI CCC'GGG
Cuts at: 0 104 1266
Size: 104 1162
SmlI C'TyrA_G
Cuts at: 0 142 1266
Size: 142 1124
StyI C'CwwG_G
Cuts at: 0 1211 1266
Size: 1211 55
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 192 357 1266
Size: 192 165 909
Fragments arranged by size:
909 192 165
XhoI C'TCGA_G
Cuts at: 0 142 1266
Size: 142 1124
XmaI C'CCGG_G
Cuts at: 0 102 1266
Size: 102 1164
Enzymes that do cut:
AcuI AfeI AflIII AleI AlfI AloI AlwNI ApaI
ApaLI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BclI BglI BlpI Bme1580I BmgBI BpmI Bpu10I BsaHI
BsaXI BseRI BseYI BsgI BsiHKAI Bsp1286I BsrDI BsrFI
BssSI BstAPI BstXI BstYI Bsu36I BtgI BtsI DraI
EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I HaeII
Hin4I HincII KasI MmeI MscI MslI MspA1I NarI
PasI PpiI PspOMI PspXI PvuII RsrII SacI SacII
SapI SfiI SfoI SmaI SmlI StyI XcmI XhoI
XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AflII AgeI AhdI
ApoI AscI AseI AsiSI AvrII BaeI BcgI BciVI
BfrBI BglII BmrI BmtI BplI BpuEI BsaI BsaAI
BsaBI BsaWI BsiEI BsiWI BsmI BsmBI BspEI BspHI
BspMI BsrBI BsrGI BssHII BstBI BstEII BstZ17I BtgZI
ClaI CspCI DraIII DrdI EagI EcoRI EcoRV FalI
FseI FspI FspAI HindIII HpaI KpnI MfeI MluI
NaeI NcoI NdeI NgoMIV NheI NotI NruI NsiI
NspI PacI PciI PflMI PfoI PmeI PmlI PpuMI
PshAI PsiI PsrI PstI PvuI SalI SanDI SbfI
ScaI SexAI SfcI SgfI SgrAI SnaBI SpeI SphI
SrfI SspI StuI SwaI TaqII TatI TstI Tth111I
XbaI XmnI ZraI