Restriction map for
pFN21AE3174
(Linear) (Six-base) MAPSORT of: KIEE3174.seq Check: 1238 from: 1 to: 1221
KIEE3174.orf
With 182 enzymes: SgfI *
December 27, 2011 16:34 ..
Acc65I G'GTAC_C
Cuts at: 0 599 1221
Size: 599 622
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 692 928 1221
Size: 692 236 293
Fragments arranged by size:
692 293 236
AseI AT'TA_AT
Cuts at: 0 457 1221
Size: 457 764
AvaI C'yCGr_G
Cuts at: 0 134 1221
Size: 134 1087
BanI G'GyrC_C
Cuts at: 0 599 1221
Size: 599 622
BanII G_rGCy'C
Cuts at: 0 887 1113 1221
Size: 887 226 108
Fragments arranged by size:
887 226 108
BbsI GAAGACnn'nnnn_
Cuts at: 0 916 1221
Size: 916 305
BciVI GTATCCnnnnn_n'
Cuts at: 0 20 413 1221
Size: 20 393 808
Fragments arranged by size:
808 393 20
BfrBI ATG'CAT
Cuts at: 0 376 1221
Size: 376 845
BglII A'GATC_T
Cuts at: 0 175 1221
Size: 175 1046
BlpI GC'TnA_GC
Cuts at: 0 1191 1221
Size: 1191 30
Bme1580I G_kGCm'C
Cuts at: 0 305 1221
Size: 305 916
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1127 1221
Size: 1127 94
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 488 1221
Size: 488 733
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 140 164 1221
Size: 140 24 1057
Fragments arranged by size:
1057 140 24
BsaI GGTCTCn'nnnn_
Cuts at: 0 353 924 1221
Size: 353 571 297
Fragments arranged by size:
571 353 297
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 697 1221
Size: 697 524
BseYI C'CCAG_C
Cuts at: 0 426 752 1221
Size: 426 326 469
Fragments arranged by size:
469 426 326
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 493 1221
Size: 493 728
BsiHKAI G_wGCw'C
Cuts at: 0 887 1113 1221
Size: 887 226 108
Fragments arranged by size:
887 226 108
BsmI GAATG_Cn'
Cuts at: 0 43 639 1221
Size: 43 596 582
Fragments arranged by size:
596 582 43
Bsp1286I G_dGCh'C
Cuts at: 0 305 887 1113 1221
Size: 305 582 226 108
Fragments arranged by size:
582 305 226 108
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 587 1221
Size: 587 634
BsrGI T'GTAC_A
Cuts at: 0 1167 1221
Size: 1167 54
BstYI r'GATC_y
Cuts at: 0 175 504 1221
Size: 175 329 717
Fragments arranged by size:
717 329 175
BtsI GCAGTG_nn'
Cuts at: 0 614 1221
Size: 614 607
DrdI GACnn_nn'nnGTC
Cuts at: 0 938 1221
Size: 938 283
EaeI y'GGCC_r
Cuts at: 0 85 1221
Size: 85 1136
EarI CTCTTCn'nnn_
Cuts at: 0 866 1101 1221
Size: 866 235 120
Fragments arranged by size:
866 235 120
EcoICRI GAG'CTC
Cuts at: 0 885 1111 1221
Size: 885 226 110
Fragments arranged by size:
885 226 110
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 488 692 928 1221
Size: 488 204 236 293
Fragments arranged by size:
488 293 236 204
EcoNI CCTnn'n_nnAGG
Cuts at: 0 74 542 1221
Size: 74 468 679
Fragments arranged by size:
679 468 74
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 58 1130 1221
Size: 58 1072 91
Fragments arranged by size:
1072 91 58
HaeII r_GCGC'y
Cuts at: 0 1191 1221
Size: 1191 30
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1196 1221
Size: 1196 25
HindIII A'AGCT_T
Cuts at: 0 1198 1221
Size: 1198 23
KpnI G_GTAC'C
Cuts at: 0 603 1221
Size: 603 618
MfeI C'AATT_G
Cuts at: 0 713 1063 1221
Size: 713 350 158
Fragments arranged by size:
713 350 158
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 35 1221
Size: 35 1186
MscI TGG'CCA
Cuts at: 0 87 1221
Size: 87 1134
MslI CAynn'nnrTG
Cuts at: 0 583 871 1221
Size: 583 288 350
Fragments arranged by size:
583 350 288
NsiI A_TGCA'T
Cuts at: 0 378 1221
Size: 378 843
NspI r_CATG'y
Cuts at: 0 612 694 1221
Size: 612 82 527
Fragments arranged by size:
612 527 82
PstI C_TGCA'G
Cuts at: 0 323 1221
Size: 323 898
SacI G_AGCT'C
Cuts at: 0 887 1113 1221
Size: 887 226 108
Fragments arranged by size:
887 226 108
SapI GCTCTTCn'nnn_
Cuts at: 0 866 1101 1221
Size: 866 235 120
Fragments arranged by size:
866 235 120
SexAI A'CCwGG_T
Cuts at: 0 933 1221
Size: 933 288
SfcI C'TryA_G
Cuts at: 0 104 319 820 1221
Size: 104 215 501 401
Fragments arranged by size:
501 401 215 104
SmaI CCC'GGG
Cuts at: 0 136 1221
Size: 136 1085
SmlI C'TyrA_G
Cuts at: 0 155 179 1221
Size: 155 24 1042
Fragments arranged by size:
1042 155 24
SphI G_CATG'C
Cuts at: 0 694 1221
Size: 694 527
StyI C'CwwG_G
Cuts at: 0 350 1221
Size: 350 871
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 500 1221
Size: 500 721
TatI w'GTAC_w
Cuts at: 0 1167 1221
Size: 1167 54
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 496 528 1221
Size: 496 32 693
Fragments arranged by size:
693 496 32
XbaI T'CTAG_A
Cuts at: 0 276 1221
Size: 276 945
XmaI C'CCGG_G
Cuts at: 0 134 1221
Size: 134 1087
XmnI GAAnn'nnTTC
Cuts at: 0 239 1221
Size: 239 982
Enzymes that do cut:
Acc65I AcuI AseI AvaI BanI BanII BbsI BciVI
BfrBI BglII BlpI Bme1580I BplI BpmI BpuEI BsaI
BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BspMI BsrGI
BstYI BtsI DrdI EaeI EarI EcoICRI Eco57MI EcoNI
FalI HaeII Hin4I HindIII KpnI MfeI MmeI MscI
MslI NsiI NspI PstI SacI SapI SexAI SfcI
SmaI SmlI SphI StyI TaqII TatI TstI XbaI
XmaI XmnI
Enzymes that do not cut:
AarI AatII AccI AclI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AscI AsiSI AvrII BaeI BamHI BbeI BbvCI BcgI
BclI BglI BmgBI BmrI BmtI Bpu10I BsaAI BsaBI
BsaHI BsaWI BsaXI BsiEI BsiWI BsmBI BspEI BspHI
BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstBI BstEII
BstXI BstZ17I Bsu36I BtgI BtgZI ClaI CspCI DraI
DraIII EagI EciI EcoO109I EcoRI EcoRV FseI FspI
FspAI HincII HpaI KasI MluI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NotI NruI PacI PasI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI
PsiI PspOMI PspXI PsrI PvuI PvuII RsrII SacII
SalI SanDI SbfI ScaI SfiI SfoI SgfI SgrAI
SnaBI SpeI SrfI SspI StuI SwaI Tth111I XcmI
XhoI ZraI