Restriction map for
pFN21AE3173
(Linear) (Six-base) MAPSORT of: KIEE3173.seq Check: 8046 from: 1 to: 1218
KIEE3173.orf
With 182 enzymes: SgfI *
March 22, 2012 16:18 ..
AatII G_ACGT'C
Cuts at: 0 1013 1218
Size: 1013 205
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 496 826 1218
Size: 496 330 392
Fragments arranged by size:
496 392 330
AfeI AGC'GCT
Cuts at: 0 1186 1218
Size: 1186 32
ApoI r'AATT_y
Cuts at: 0 339 836 1218
Size: 339 497 382
Fragments arranged by size:
497 382 339
AseI AT'TA_AT
Cuts at: 0 716 1218
Size: 716 502
AvaI C'yCGr_G
Cuts at: 0 949 985 1218
Size: 949 36 233
Fragments arranged by size:
949 233 36
AvrII C'CTAG_G
Cuts at: 0 18 1042 1218
Size: 18 1024 176
Fragments arranged by size:
1024 176 18
BanI G'GyrC_C
Cuts at: 0 916 1218
Size: 916 302
BanII G_rGCy'C
Cuts at: 0 396 643 1218
Size: 396 247 575
Fragments arranged by size:
575 396 247
BbsI GAAGACnn'nnnn_
Cuts at: 0 282 455 753 1218
Size: 282 173 298 465
Fragments arranged by size:
465 298 282 173
BclI T'GATC_A
Cuts at: 0 324 1218
Size: 324 894
BglII A'GATC_T
Cuts at: 0 1151 1218
Size: 1151 67
BlpI GC'TnA_GC
Cuts at: 0 112 1218
Size: 112 1106
BmgBI CAC'GTC
Cuts at: 0 453 1218
Size: 453 765
BmrI ACTGGGnnnn_n'
Cuts at: 0 522 1218
Size: 522 696
BsaHI Gr'CG_yC
Cuts at: 0 1010 1218
Size: 1010 208
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 895 910 913 1218
Size: 895 15 3 305
Fragments arranged by size:
895 305 15 3
BseYI C'CCAG_C
Cuts at: 0 800 1218
Size: 800 418
BsiHKAI G_wGCw'C
Cuts at: 0 396 1218
Size: 396 822
Bsp1286I G_dGCh'C
Cuts at: 0 396 643 1218
Size: 396 247 575
Fragments arranged by size:
575 396 247
BspHI T'CATG_A
Cuts at: 0 840 1218
Size: 840 378
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 308 1218
Size: 308 910
BsrFI r'CCGG_y
Cuts at: 0 545 1218
Size: 545 673
BstYI r'GATC_y
Cuts at: 0 1151 1218
Size: 1151 67
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 926 1218
Size: 926 292
BtsI GCAGTG_nn'
Cuts at: 0 393 1218
Size: 393 825
DraIII CAC_nnn'GTG
Cuts at: 0 927 1218
Size: 927 291
EaeI y'GGCC_r
Cuts at: 0 455 1203 1218
Size: 455 748 15
Fragments arranged by size:
748 455 15
EcoICRI GAG'CTC
Cuts at: 0 394 1218
Size: 394 824
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 496 826 1218
Size: 496 330 392
Fragments arranged by size:
496 392 330
EcoO109I rG'GnC_Cy
Cuts at: 0 23 1218
Size: 23 1195
EcoRI G'AATT_C
Cuts at: 0 339 836 1218
Size: 339 497 382
Fragments arranged by size:
497 382 339
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 81 777 1218
Size: 81 696 441
Fragments arranged by size:
696 441 81
HaeII r_GCGC'y
Cuts at: 0 1142 1188 1218
Size: 1142 46 30
Fragments arranged by size:
1142 46 30
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 413 445 1218
Size: 413 32 773
Fragments arranged by size:
773 413 32
MscI TGG'CCA
Cuts at: 0 1205 1218
Size: 1205 13
MslI CAynn'nnrTG
Cuts at: 0 1091 1218
Size: 1091 127
MspA1I CmG'CkG
Cuts at: 0 108 804 1218
Size: 108 696 414
Fragments arranged by size:
696 414 108
NspI r_CATG'y
Cuts at: 0 252 543 1218
Size: 252 291 675
Fragments arranged by size:
675 291 252
PflMI CCAn_nnn'nTGG
Cuts at: 0 534 927 1218
Size: 534 393 291
Fragments arranged by size:
534 393 291
PfoI T'CCnGG_A
Cuts at: 0 764 1218
Size: 764 454
PpuMI rG'GwC_Cy
Cuts at: 0 23 1218
Size: 23 1195
PshAI GACnn'nnGTC
Cuts at: 0 821 1218
Size: 821 397
PvuII CAG'CTG
Cuts at: 0 804 1218
Size: 804 414
SacI G_AGCT'C
Cuts at: 0 396 1218
Size: 396 822
SfcI C'TryA_G
Cuts at: 0 644 819 1218
Size: 644 175 399
Fragments arranged by size:
644 399 175
SmaI CCC'GGG
Cuts at: 0 951 987 1218
Size: 951 36 231
Fragments arranged by size:
951 231 36
StyI C'CwwG_G
Cuts at: 0 18 1042 1218
Size: 18 1024 176
Fragments arranged by size:
1024 176 18
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 934 1218
Size: 934 284
TatI w'GTAC_w
Cuts at: 0 491 1055 1218
Size: 491 564 163
Fragments arranged by size:
564 491 163
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 507 539 859 891 1218
Size: 507 32 320 32 327
Fragments arranged by size:
507 327 320 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 385 450 1218
Size: 385 65 768
Fragments arranged by size:
768 385 65
XmaI C'CCGG_G
Cuts at: 0 949 985 1218
Size: 949 36 233
Fragments arranged by size:
949 233 36
XmnI GAAnn'nnTTC
Cuts at: 0 64 760 1218
Size: 64 696 458
Fragments arranged by size:
696 458 64
ZraI GAC'GTC
Cuts at: 0 1011 1218
Size: 1011 207
Enzymes that do cut:
AatII AcuI AfeI ApoI AseI AvaI AvrII BanI
BanII BbsI BclI BglII BlpI BmgBI BmrI BsaHI
BseRI BseYI BsiHKAI Bsp1286I BspHI BspMI BsrFI BstYI
BtgZI BtsI DraIII EaeI EcoICRI Eco57MI EcoO109I EcoRI
FalI HaeII Hin4I MscI MslI MspA1I NspI PflMI
PfoI PpuMI PshAI PvuII SacI SfcI SmaI StyI
TaqII TatI TstI XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AccI Acc65I AclI AflII AflIII AgeI AhdI
AleI AlfI AloI AlwNI ApaI ApaLI AscI AsiSI
BaeI BamHI BbeI BbvCI BcgI BciVI BfrBI BglI
Bme1580I BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaWI BsaXI BsgI BsiEI BsiWI BsmI BsmBI
BspEI BsrBI BsrDI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstZ17I Bsu36I BtgI ClaI CspCI DraI
DrdI EagI EarI EciI EcoNI EcoRV FseI FspI
FspAI HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI NaeI NarI NcoI NdeI NgoMIV NheI NotI
NruI NsiI PacI PasI PciI PmeI PmlI PpiI
PsiI PspOMI PspXI PsrI PstI PvuI RsrII SacII
SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI
SgfI SgrAI SmlI SnaBI SpeI SphI SrfI SspI
StuI SwaI Tth111I XbaI XhoI