Restriction map for
pFN21AE3171
(Linear) (Six-base) MAPSORT of: KIEE3171.seq Check: 3643 from: 1 to: 1209
KIEE3171.orf
With 182 enzymes: SgfI *
December 27, 2011 16:34 ..
AatII G_ACGT'C
Cuts at: 0 887 1209
Size: 887 322
AccI GT'mk_AC
Cuts at: 0 44 1209
Size: 44 1165
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 641 1209
Size: 641 568
AhdI GACnn_n'nnGTC
Cuts at: 0 771 1209
Size: 771 438
AleI CACnn'nnGTG
Cuts at: 0 61 1209
Size: 61 1148
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 193 1209
Size: 193 1016
ApoI r'AATT_y
Cuts at: 0 591 1209
Size: 591 618
AvaI C'yCGr_G
Cuts at: 0 816 1209
Size: 816 393
BamHI G'GATC_C
Cuts at: 0 582 1209
Size: 582 627
BbsI GAAGACnn'nnnn_
Cuts at: 0 904 1209
Size: 904 305
BbvCI CC'TCA_GC
Cuts at: 0 905 1209
Size: 905 304
BfrBI ATG'CAT
Cuts at: 0 1030 1209
Size: 1030 179
BmrI ACTGGGnnnn_n'
Cuts at: 0 1118 1209
Size: 1118 91
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 256 773 1209
Size: 256 517 436
Fragments arranged by size:
517 436 256
Bpu10I CC'TnA_GC
Cuts at: 0 905 1209
Size: 905 304
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 295 424 1209
Size: 295 129 785
Fragments arranged by size:
785 295 129
BsaBI GATnn'nnATC
Cuts at: 0 1151 1209
Size: 1151 58
BsaHI Gr'CG_yC
Cuts at: 0 884 1209
Size: 884 325
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 147 177 746 776 1209
Size: 147 30 569 30 433
Fragments arranged by size:
569 433 147 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 27 283 862 1209
Size: 27 256 579 347
Fragments arranged by size:
579 347 256 27
BsiHKAI G_wGCw'C
Cuts at: 0 58 101 872 1209
Size: 58 43 771 337
Fragments arranged by size:
771 337 58 43
Bsp1286I G_dGCh'C
Cuts at: 0 58 101 872 1209
Size: 58 43 771 337
Fragments arranged by size:
771 337 58 43
BsrDI GCAATG_nn'
Cuts at: 0 243 1209
Size: 243 966
BsrFI r'CCGG_y
Cuts at: 0 6 226 1209
Size: 6 220 983
Fragments arranged by size:
983 220 6
BstXI CCAn_nnnn'nTGG
Cuts at: 0 436 1209
Size: 436 773
BstYI r'GATC_y
Cuts at: 0 203 582 1209
Size: 203 379 627
Fragments arranged by size:
627 379 203
Bsu36I CC'TnA_GG
Cuts at: 0 494 1209
Size: 494 715
BtgI C'CryG_G
Cuts at: 0 30 49 1209
Size: 30 19 1160
Fragments arranged by size:
1160 30 19
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 928 1209
Size: 928 281
EaeI y'GGCC_r
Cuts at: 0 228 354 932 1209
Size: 228 126 578 277
Fragments arranged by size:
578 277 228 126
EarI CTCTTCn'nnn_
Cuts at: 0 338 1209
Size: 338 871
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 94 235 1209
Size: 94 141 974
Fragments arranged by size:
974 141 94
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 256 641 773 1209
Size: 256 385 132 436
Fragments arranged by size:
436 385 256 132
EcoNI CCTnn'n_nnAGG
Cuts at: 0 79 220 877 1209
Size: 79 141 657 332
Fragments arranged by size:
657 332 141 79
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 646 1209
Size: 646 563
FseI GG_CCGG'CC
Cuts at: 0 230 1209
Size: 230 979
FspI TGC'GCA
Cuts at: 0 169 1209
Size: 169 1040
HaeII r_GCGC'y
Cuts at: 0 924 1209
Size: 924 285
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 599 631 757 789 836 868 1209
Size: 599 32 126 32 47 32 341
Fragments arranged by size:
599 341 126 47 32 32 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 807 1209
Size: 807 402
MscI TGG'CCA
Cuts at: 0 356 1209
Size: 356 853
MslI CAynn'nnrTG
Cuts at: 0 61 1209
Size: 61 1148
MspA1I CmG'CkG
Cuts at: 0 32 51 1209
Size: 32 19 1158
Fragments arranged by size:
1158 32 19
NaeI GCC'GGC
Cuts at: 0 228 1209
Size: 228 981
NgoMIV G'CCGG_C
Cuts at: 0 226 1209
Size: 226 983
NsiI A_TGCA'T
Cuts at: 0 1032 1209
Size: 1032 177
NspI r_CATG'y
Cuts at: 0 977 1209
Size: 977 232
PsiI TTA'TAA
Cuts at: 0 674 1209
Size: 674 535
PstI C_TGCA'G
Cuts at: 0 710 1209
Size: 710 499
SacII CC_GC'GG
Cuts at: 0 33 52 1209
Size: 33 19 1157
Fragments arranged by size:
1157 33 19
SfcI C'TryA_G
Cuts at: 0 706 1209
Size: 706 503
SmlI C'TyrA_G
Cuts at: 0 274 403 1209
Size: 274 129 806
Fragments arranged by size:
806 274 129
SpeI A'CTAG_T
Cuts at: 0 379 1209
Size: 379 830
SphI G_CATG'C
Cuts at: 0 977 1209
Size: 977 232
StuI AGG'CCT
Cuts at: 0 215 1209
Size: 215 994
StyI C'CwwG_G
Cuts at: 0 116 435 1007 1209
Size: 116 319 572 202
Fragments arranged by size:
572 319 202 116
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 147 179 446 478 746 778 1209
Size: 147 32 267 32 268 32 431
Fragments arranged by size:
431 268 267 147 32 32 32
XmnI GAAnn'nnTTC
Cuts at: 0 412 1209
Size: 412 797
ZraI GAC'GTC
Cuts at: 0 885 1209
Size: 885 324
Enzymes that do cut:
AatII AccI AcuI AhdI AleI AlfI ApoI AvaI
BamHI BbsI BbvCI BfrBI BmrI BpmI Bpu10I BpuEI
BsaBI BsaHI BsaXI BseRI BsiHKAI Bsp1286I BsrDI BsrFI
BstXI BstYI Bsu36I BtgI BtgZI EaeI EarI EciI
Eco57MI EcoNI FalI FseI FspI HaeII Hin4I MmeI
MscI MslI MspA1I NaeI NgoMIV NsiI NspI PsiI
PstI SacII SfcI SmlI SpeI SphI StuI StyI
TstI XmnI ZraI
Enzymes that do not cut:
AarI Acc65I AclI AfeI AflII AflIII AgeI AloI
AlwNI ApaI ApaLI AscI AseI AsiSI AvrII BaeI
BanI BanII BbeI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmtI BplI BsaI BsaAI BsaWI
BseYI BsgI BsiEI BsiWI BsmI BsmBI BspEI BspHI
BspMI BsrBI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstZ17I BtsI ClaI CspCI DraI DraIII DrdI EagI
EcoICRI EcoO109I EcoRI EcoRV FspAI HincII HindIII HpaI
KasI KpnI MfeI MluI NarI NcoI NdeI NheI
NotI NruI PacI PasI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PshAI PspOMI PspXI PsrI PvuI
PvuII RsrII SacI SalI SanDI SapI SbfI ScaI
SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SrfI
SspI SwaI TaqII TatI Tth111I XbaI XcmI XhoI
XmaI