Restriction map for
pFN21AE3169
(Linear) (Six-base) MAPSORT of: KIEE3169.seq Check: 8930 from: 1 to: 1197
KIEE3169.orf
With 182 enzymes: SgfI *
July 3, 2012 17:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 376 1197
Size: 376 821
AfeI AGC'GCT
Cuts at: 0 235 457 876 1197
Size: 235 222 419 321
Fragments arranged by size:
419 321 235 222
AflIII A'CryG_T
Cuts at: 0 461 1197
Size: 461 736
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 821 1197
Size: 821 376
AlwNI CAG_nnn'CTG
Cuts at: 0 81 391 849 1197
Size: 81 310 458 348
Fragments arranged by size:
458 348 310 81
ApaI G_GGCC'C
Cuts at: 0 308 549 795 1197
Size: 308 241 246 402
Fragments arranged by size:
402 308 246 241
BanI G'GyrC_C
Cuts at: 0 298 375 708 832 1092 1197
Size: 298 77 333 124 260 105
Fragments arranged by size:
333 298 260 124 105 77
BanII G_rGCy'C
Cuts at: 0 308 407 526 549 795 1010 1197
Size: 308 99 119 23 246 215 187
Fragments arranged by size:
308 246 215 187 119 99 23
BbeI G_GCGC'C
Cuts at: 0 302 379 1197
Size: 302 77 818
Fragments arranged by size:
818 302 77
BbsI GAAGACnn'nnnn_
Cuts at: 0 479 1197
Size: 479 718
BbvCI CC'TCA_GC
Cuts at: 0 871 1197
Size: 871 326
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 115 149 1197
Size: 115 34 1048
Fragments arranged by size:
1048 115 34
BclI T'GATC_A
Cuts at: 0 1064 1197
Size: 1064 133
BglI GCCn_nnn'nGGC
Cuts at: 0 10 706 1197
Size: 10 696 491
Fragments arranged by size:
696 491 10
Bme1580I G_kGCm'C
Cuts at: 0 308 549 795 1095 1197
Size: 308 241 246 300 102
Fragments arranged by size:
308 300 246 241 102
BmgBI CAC'GTC
Cuts at: 0 493 1197
Size: 493 704
BmrI ACTGGGnnnn_n'
Cuts at: 0 898 1197
Size: 898 299
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 510 1197
Size: 510 687
Bpu10I CC'TnA_GC
Cuts at: 0 871 1197
Size: 871 326
BsaI GGTCTCn'nnnn_
Cuts at: 0 334 1197
Size: 334 863
BsaAI yAC'GTr
Cuts at: 0 462 1197
Size: 462 735
BsaBI GATnn'nnATC
Cuts at: 0 82 1188 1197
Size: 82 1106 9
Fragments arranged by size:
1106 82 9
BsaHI Gr'CG_yC
Cuts at: 0 299 376 1197
Size: 299 77 821
Fragments arranged by size:
821 299 77
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 310 340 586 616 1140 1170 1197
Size: 310 30 246 30 524 30 27
Fragments arranged by size:
524 310 246 30 30 30 27
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 352 415 728 1099 1197
Size: 352 63 313 371 98
Fragments arranged by size:
371 352 313 98 63
BseYI C'CCAG_C
Cuts at: 0 391 452 666 794 1107 1197
Size: 391 61 214 128 313 90
Fragments arranged by size:
391 313 214 128 90 61
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 247 515 543 1197
Size: 247 268 28 654
Fragments arranged by size:
654 268 247 28
BsiEI CG_ry'CG
Cuts at: 0 1182 1197
Size: 1182 15
Bsp1286I G_dGCh'C
Cuts at: 0 308 407 526 549 795 1010 1095 1197
Size: 308 99 119 23 246 215 85 102
Fragments arranged by size:
308 246 215 119 102 99 85 23
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 376 1197
Size: 376 821
BsrBI CCG'CTC
Cuts at: 0 153 1116 1197
Size: 153 963 81
Fragments arranged by size:
963 153 81
BsrDI GCAATG_nn'
Cuts at: 0 1067 1197
Size: 1067 130
BsrFI r'CCGG_y
Cuts at: 0 627 829 978 1197
Size: 627 202 149 219
Fragments arranged by size:
627 219 202 149
BssHII G'CGCG_C
Cuts at: 0 96 122 1197
Size: 96 26 1075
Fragments arranged by size:
1075 96 26
BstBI TT'CG_AA
Cuts at: 0 65 1197
Size: 65 1132
BstYI r'GATC_y
Cuts at: 0 77 1197
Size: 77 1120
BtgI C'CryG_G
Cuts at: 0 311 329 541 583 1016 1197
Size: 311 18 212 42 433 181
Fragments arranged by size:
433 311 212 181 42 18
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 159 1197
Size: 159 1038
BtsI GCAGTG_nn'
Cuts at: 0 676 1049 1197
Size: 676 373 148
Fragments arranged by size:
676 373 148
EaeI y'GGCC_r
Cuts at: 0 827 851 1179 1197
Size: 827 24 328 18
Fragments arranged by size:
827 328 24 18
EagI C'GGCC_G
Cuts at: 0 1179 1197
Size: 1179 18
EarI CTCTTCn'nnn_
Cuts at: 0 62 329 426 1197
Size: 62 267 97 771
Fragments arranged by size:
771 267 97 62
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 412 1197
Size: 412 785
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 510 1197
Size: 510 687
EcoNI CCTnn'n_nnAGG
Cuts at: 0 841 1197
Size: 841 356
EcoO109I rG'GnC_Cy
Cuts at: 0 867 1197
Size: 867 330
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 72 1197
Size: 72 1125
FspI TGC'GCA
Cuts at: 0 46 1197
Size: 46 1151
HaeII r_GCGC'y
Cuts at: 0 237 255 302 379 446 459 878 1197
Size: 237 18 47 77 67 13 419 319
Fragments arranged by size:
419 319 237 77 67 47 18 13
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 310 342 586 618 997 1029 1197
Size: 310 32 244 32 379 32 168
Fragments arranged by size:
379 310 244 168 32 32 32
HincII GTy'rAC
Cuts at: 0 909 1197
Size: 909 288
KasI G'GCGC_C
Cuts at: 0 298 375 1197
Size: 298 77 822
Fragments arranged by size:
822 298 77
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 449 1197
Size: 449 748
MscI TGG'CCA
Cuts at: 0 853 1197
Size: 853 344
MspA1I CmG'CkG
Cuts at: 0 203 313 331 636 1197
Size: 203 110 18 305 561
Fragments arranged by size:
561 305 203 110 18
NaeI GCC'GGC
Cuts at: 0 831 1197
Size: 831 366
NarI GG'CG_CC
Cuts at: 0 299 376 1197
Size: 299 77 821
Fragments arranged by size:
821 299 77
NcoI C'CATG_G
Cuts at: 0 541 1197
Size: 541 656
NgoMIV G'CCGG_C
Cuts at: 0 829 1197
Size: 829 368
PfoI T'CCnGG_A
Cuts at: 0 818 1197
Size: 818 379
PspOMI G'GGCC_C
Cuts at: 0 304 545 791 1197
Size: 304 241 246 406
Fragments arranged by size:
406 304 246 241
PstI C_TGCA'G
Cuts at: 0 626 1197
Size: 626 571
PvuII CAG'CTG
Cuts at: 0 203 636 1197
Size: 203 433 561
Fragments arranged by size:
561 433 203
SacII CC_GC'GG
Cuts at: 0 314 332 1197
Size: 314 18 865
Fragments arranged by size:
865 314 18
SfcI C'TryA_G
Cuts at: 0 622 1197
Size: 622 575
SfoI GGC'GCC
Cuts at: 0 300 377 1197
Size: 300 77 820
Fragments arranged by size:
820 300 77
StuI AGG'CCT
Cuts at: 0 963 1197
Size: 963 234
StyI C'CwwG_G
Cuts at: 0 260 541 590 986 1075 1197
Size: 260 281 49 396 89 122
Fragments arranged by size:
396 281 260 122 89 49
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 503 765 1197
Size: 503 262 432
Fragments arranged by size:
503 432 262
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 822 1197
Size: 822 375
XmnI GAAnn'nnTTC
Cuts at: 0 244 1197
Size: 244 953
Enzymes that do cut:
AarI AfeI AflIII AlfI AlwNI ApaI BanI BanII
BbeI BbsI BbvCI BcgI BclI BglI Bme1580I BmgBI
BmrI BpmI Bpu10I BsaI BsaAI BsaBI BsaHI BsaXI
BseRI BseYI BsgI BsiEI Bsp1286I BspMI BsrBI BsrDI
BsrFI BssHII BstBI BstYI BtgI BtgZI BtsI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I FalI FspI
HaeII Hin4I HincII KasI MmeI MscI MspA1I NaeI
NarI NcoI NgoMIV PfoI PspOMI PstI PvuII SacII
SfcI SfoI StuI StyI TaqII XcmI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AcuI AflII AgeI AhdI
AleI AloI ApaLI ApoI AscI AseI AsiSI AvaI
AvrII BaeI BamHI BciVI BfrBI BglII BlpI BmtI
BplI BpuEI BsaWI BsiHKAI BsiWI BsmI BsmBI BspEI
BspHI BsrGI BssSI BstAPI BstEII BstXI BstZ17I Bsu36I
ClaI CspCI DraI DraIII DrdI EcoICRI EcoRI EcoRV
FseI FspAI HindIII HpaI KpnI MfeI MluI MslI
NdeI NheI NotI NruI NsiI NspI PacI PasI
PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI
PspXI PsrI PvuI RsrII SacI SalI SanDI SapI
SbfI ScaI SexAI SfiI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SrfI SspI SwaI TatI TstI
Tth111I XbaI XhoI XmaI ZraI