Restriction map for
pFN21AE3168
(Linear) (Six-base) MAPSORT of: KIEE3168.seq Check: 7568 from: 1 to: 573
KIEE3168.orf
With 182 enzymes: SgfI *
July 3, 2012 17:24 ..
AccI GT'mk_AC
Cuts at: 0 446 573
Size: 446 127
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 136 573
Size: 136 437
AfeI AGC'GCT
Cuts at: 0 412 573
Size: 412 161
ApaI G_GGCC'C
Cuts at: 0 158 277 404 573
Size: 158 119 127 169
Fragments arranged by size:
169 158 127 119
AvaI C'yCGr_G
Cuts at: 0 276 403 573
Size: 276 127 170
Fragments arranged by size:
276 170 127
BamHI G'GATC_C
Cuts at: 0 78 573
Size: 78 495
BanII G_rGCy'C
Cuts at: 0 158 215 277 331 348 404 455 573
Size: 158 57 62 54 17 56 51 118
Fragments arranged by size:
158 118 62 57 56 54 51 17
BbsI GAAGACnn'nnnn_
Cuts at: 0 3 487 573
Size: 3 484 86
Fragments arranged by size:
484 86 3
BciVI GTATCCnnnnn_n'
Cuts at: 0 186 573
Size: 186 387
Bme1580I G_kGCm'C
Cuts at: 0 158 277 324 404 573
Size: 158 119 47 80 169
Fragments arranged by size:
169 158 119 80 47
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 487 573
Size: 487 86
BsaHI Gr'CG_yC
Cuts at: 0 371 425 573
Size: 371 54 148
Fragments arranged by size:
371 148 54
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 460 490 573
Size: 460 30 83
Fragments arranged by size:
460 83 30
BseYI C'CCAG_C
Cuts at: 0 71 158 573
Size: 71 87 415
Fragments arranged by size:
415 87 71
BsiEI CG_ry'CG
Cuts at: 0 27 573
Size: 27 546
BsiHKAI G_wGCw'C
Cuts at: 0 215 473 573
Size: 215 258 100
Fragments arranged by size:
258 215 100
BsmBI CGTCTCn'nnnn_
Cuts at: 0 259 573
Size: 259 314
Bsp1286I G_dGCh'C
Cuts at: 0 158 215 277 324 331 348 404 455
Size: 158 57 62 47 7 17 56 51
Cuts at: 455 473 573
Size: 18 100
Fragments arranged by size:
158 100 62 57 56 51 47 18
17 7
BsrFI r'CCGG_y
Cuts at: 0 22 356 573
Size: 22 334 217
Fragments arranged by size:
334 217 22
BssSI C'ACGA_G
Cuts at: 0 259 573
Size: 259 314
BstXI CCAn_nnnn'nTGG
Cuts at: 0 150 573
Size: 150 423
BstYI r'GATC_y
Cuts at: 0 78 432 573
Size: 78 354 141
Fragments arranged by size:
354 141 78
Bsu36I CC'TnA_GG
Cuts at: 0 89 573
Size: 89 484
BtsI GCAGTG_nn'
Cuts at: 0 484 573
Size: 484 89
EaeI y'GGCC_r
Cuts at: 0 24 302 573
Size: 24 278 271
Fragments arranged by size:
278 271 24
EagI C'GGCC_G
Cuts at: 0 24 573
Size: 24 549
EarI CTCTTCn'nnn_
Cuts at: 0 329 573
Size: 329 244
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 166 573
Size: 166 407
EcoICRI GAG'CTC
Cuts at: 0 213 573
Size: 213 360
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 136 487 573
Size: 136 351 86
Fragments arranged by size:
351 136 86
EcoO109I rG'GnC_Cy
Cuts at: 0 120 154 155 273 573
Size: 120 34 1 118 300
Fragments arranged by size:
300 120 118 34 1
HaeII r_GCGC'y
Cuts at: 0 414 573
Size: 414 159
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 273 573
Size: 273 300
MslI CAynn'nnrTG
Cuts at: 0 263 573
Size: 263 310
MspA1I CmG'CkG
Cuts at: 0 71 162 573
Size: 71 91 411
Fragments arranged by size:
411 91 71
NaeI GCC'GGC
Cuts at: 0 24 358 573
Size: 24 334 215
Fragments arranged by size:
334 215 24
NgoMIV G'CCGG_C
Cuts at: 0 22 356 573
Size: 22 334 217
Fragments arranged by size:
334 217 22
PflMI CCAn_nnn'nTGG
Cuts at: 0 555 573
Size: 555 18
PfoI T'CCnGG_A
Cuts at: 0 206 392 573
Size: 206 186 181
Fragments arranged by size:
206 186 181
PpuMI rG'GwC_Cy
Cuts at: 0 120 573
Size: 120 453
PspOMI G'GGCC_C
Cuts at: 0 154 273 400 573
Size: 154 119 127 173
Fragments arranged by size:
173 154 127 119
SacI G_AGCT'C
Cuts at: 0 215 573
Size: 215 358
SmaI CCC'GGG
Cuts at: 0 278 573
Size: 278 295
SrfI GCCC'GGGC
Cuts at: 0 278 573
Size: 278 295
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 63 543 573
Size: 63 480 30
Fragments arranged by size:
480 63 30
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 460 492 573
Size: 460 32 81
Fragments arranged by size:
460 81 32
XmaI C'CCGG_G
Cuts at: 0 276 573
Size: 276 297
Enzymes that do cut:
AccI AcuI AfeI ApaI AvaI BamHI BanII BbsI
BciVI Bme1580I BpmI BsaHI BsaXI BseYI BsiEI BsiHKAI
BsmBI Bsp1286I BsrFI BssSI BstXI BstYI Bsu36I BtsI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I HaeII
MmeI MslI MspA1I NaeI NgoMIV PflMI PfoI PpuMI
PspOMI SacI SmaI SrfI TaqII TstI XmaI
Enzymes that do not cut:
AarI AatII Acc65I AclI AflII AflIII AgeI AhdI
AleI AlfI AloI AlwNI ApaLI ApoI AscI AseI
AsiSI AvrII BaeI BanI BbeI BbvCI BcgI BclI
BfrBI BglI BglII BlpI BmgBI BmrI BmtI BplI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaWI BseRI BsgI
BsiWI BsmI BspEI BspHI BspMI BsrBI BsrDI BsrGI
BssHII BstAPI BstBI BstEII BstZ17I BtgI BtgZI ClaI
CspCI DraI DraIII DrdI EcoNI EcoRI EcoRV FalI
FseI FspI FspAI Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MscI NarI NcoI NdeI NheI
NotI NruI NsiI NspI PacI PasI PciI PmeI
PmlI PpiI PshAI PsiI PspXI PsrI PstI PvuI
PvuII RsrII SacII SalI SanDI SapI SbfI ScaI
SexAI SfcI SfiI SfoI SgfI SgrAI SmlI SnaBI
SpeI SphI SspI StuI StyI SwaI TatI Tth111I
XbaI XcmI XhoI XmnI ZraI