Restriction map for
pFN21AE3165
(Linear) (Six-base) MAPSORT of: KIEE3165.seq Check: 2304 from: 1 to: 1161
KIEE3165.orf
With 182 enzymes: SgfI *
March 22, 2012 16:17 ..
AccI GT'mk_AC
Cuts at: 0 780 1161
Size: 780 381
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 33 581 905 1161
Size: 33 548 324 256
Fragments arranged by size:
548 324 256 33
AhdI GACnn_n'nnGTC
Cuts at: 0 345 1161
Size: 345 816
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 773 1161
Size: 773 388
AlwNI CAG_nnn'CTG
Cuts at: 0 56 89 1090 1161
Size: 56 33 1001 71
Fragments arranged by size:
1001 71 56 33
ApoI r'AATT_y
Cuts at: 0 688 916 1161
Size: 688 228 245
Fragments arranged by size:
688 245 228
BbsI GAAGACnn'nnnn_
Cuts at: 0 100 323 587 950 972 1161
Size: 100 223 264 363 22 189
Fragments arranged by size:
363 264 223 189 100 22
BbvCI CC'TCA_GC
Cuts at: 0 82 1161
Size: 82 1079
BciVI GTATCCnnnnn_n'
Cuts at: 0 1074 1161
Size: 1074 87
BclI T'GATC_A
Cuts at: 0 512 798 1161
Size: 512 286 363
Fragments arranged by size:
512 363 286
BglI GCCn_nnn'nGGC
Cuts at: 0 633 1161
Size: 633 528
BglII A'GATC_T
Cuts at: 0 357 1161
Size: 357 804
BmrI ACTGGGnnnn_n'
Cuts at: 0 66 1161
Size: 66 1095
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 88 1161
Size: 88 1073
Bpu10I CC'TnA_GC
Cuts at: 0 82 1161
Size: 82 1079
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 980 1161
Size: 980 181
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 163 1161
Size: 163 998
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 779 1161
Size: 779 382
BsiWI C'GTAC_G
Cuts at: 0 133 1161
Size: 133 1028
BsmI GAATG_Cn'
Cuts at: 0 1019 1161
Size: 1019 142
BspHI T'CATG_A
Cuts at: 0 228 1035 1161
Size: 228 807 126
Fragments arranged by size:
807 228 126
BsrDI GCAATG_nn'
Cuts at: 0 941 1161
Size: 941 220
BsrFI r'CCGG_y
Cuts at: 0 812 1161
Size: 812 349
BsrGI T'GTAC_A
Cuts at: 0 195 1161
Size: 195 966
BstXI CCAn_nnnn'nTGG
Cuts at: 0 293 1161
Size: 293 868
BstYI r'GATC_y
Cuts at: 0 282 315 357 942 1161
Size: 282 33 42 585 219
Fragments arranged by size:
585 282 219 42 33
BtgI C'CryG_G
Cuts at: 0 1066 1161
Size: 1066 95
EaeI y'GGCC_r
Cuts at: 0 74 164 1161
Size: 74 90 997
Fragments arranged by size:
997 90 74
EarI CTCTTCn'nnn_
Cuts at: 0 356 447 740 1161
Size: 356 91 293 421
Fragments arranged by size:
421 356 293 91
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 33 88 581 905 1161
Size: 33 55 493 324 256
Fragments arranged by size:
493 324 256 55 33
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 473 1161
Size: 473 688
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 331 363 644 676 762 794 1161
Size: 331 32 281 32 86 32 367
Fragments arranged by size:
367 331 281 86 32 32 32
HincII GTy'rAC
Cuts at: 0 339 765 781 1161
Size: 339 426 16 380
Fragments arranged by size:
426 380 339 16
HpaI GTT'AAC
Cuts at: 0 765 1161
Size: 765 396
MslI CAynn'nnrTG
Cuts at: 0 755 1161
Size: 755 406
MspA1I CmG'CkG
Cuts at: 0 23 86 619 1161
Size: 23 63 533 542
Fragments arranged by size:
542 533 63 23
NcoI C'CATG_G
Cuts at: 0 1066 1161
Size: 1066 95
PflMI CCAn_nnn'nTGG
Cuts at: 0 619 952 1161
Size: 619 333 209
Fragments arranged by size:
619 333 209
PfoI T'CCnGG_A
Cuts at: 0 1076 1161
Size: 1076 85
PvuII CAG'CTG
Cuts at: 0 619 1161
Size: 619 542
SalI G'TCGA_C
Cuts at: 0 779 1161
Size: 779 382
SapI GCTCTTCn'nnn_
Cuts at: 0 447 740 1161
Size: 447 293 421
Fragments arranged by size:
447 421 293
SexAI A'CCwGG_T
Cuts at: 0 549 1161
Size: 549 612
SfcI C'TryA_G
Cuts at: 0 426 1161
Size: 426 735
SmlI C'TyrA_G
Cuts at: 0 995 1161
Size: 995 166
StyI C'CwwG_G
Cuts at: 0 1066 1161
Size: 1066 95
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1079 1161
Size: 1079 82
TatI w'GTAC_w
Cuts at: 0 195 250 1161
Size: 195 55 911
Fragments arranged by size:
911 195 55
XbaI T'CTAG_A
Cuts at: 0 360 1161
Size: 360 801
XmnI GAAnn'nnTTC
Cuts at: 0 97 456 1161
Size: 97 359 705
Fragments arranged by size:
705 359 97
Enzymes that do cut:
AccI AcuI AhdI AlfI AlwNI ApoI BbsI BbvCI
BciVI BclI BglI BglII BmrI BpmI Bpu10I BpuEI
BseRI BsgI BsiWI BsmI BspHI BsrDI BsrFI BsrGI
BstXI BstYI BtgI EaeI EarI Eco57MI FalI Hin4I
HincII HpaI MslI MspA1I NcoI PflMI PfoI PvuII
SalI SapI SexAI SfcI SmlI StyI TaqII TatI
XbaI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AleI AloI ApaI ApaLI AscI AseI AsiSI AvaI
AvrII BaeI BamHI BanI BanII BbeI BcgI BfrBI
BlpI Bme1580I BmgBI BmtI BplI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseYI BsiEI BsiHKAI BsmBI Bsp1286I
BspEI BspMI BsrBI BssHII BssSI BstAPI BstBI BstEII
BstZ17I Bsu36I BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EagI EciI EcoICRI EcoNI EcoO109I EcoRI EcoRV
FseI FspI FspAI HaeII HindIII KasI KpnI MfeI
MluI MmeI MscI NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI NspI PacI PasI PciI PmeI
PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI
PstI PvuI RsrII SacI SacII SanDI SbfI ScaI
SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI
SrfI SspI StuI SwaI TstI Tth111I XcmI XhoI
XmaI ZraI