Restriction map for
pFN21AE3164
(Linear) (Six-base) MAPSORT of: KIEE3164.seq Check: 4441 from: 1 to: 1155
KIEE3164.orf
With 182 enzymes: SgfI *
December 27, 2011 16:33 ..
AccI GT'mk_AC
Cuts at: 0 992 1155
Size: 992 163
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 358 544 1106 1155
Size: 358 186 562 49
Fragments arranged by size:
562 358 186 49
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 402 434 1155
Size: 402 32 721
Fragments arranged by size:
721 402 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1038 1155
Size: 1038 117
ApaI G_GGCC'C
Cuts at: 0 639 1155
Size: 639 516
ApaLI G'TGCA_C
Cuts at: 0 696 757 1155
Size: 696 61 398
Fragments arranged by size:
696 398 61
ApoI r'AATT_y
Cuts at: 0 578 1155
Size: 578 577
BanI G'GyrC_C
Cuts at: 0 913 1155
Size: 913 242
BanII G_rGCy'C
Cuts at: 0 639 1155
Size: 639 516
BbeI G_GCGC'C
Cuts at: 0 917 1155
Size: 917 238
BbsI GAAGACnn'nnnn_
Cuts at: 0 108 346 1155
Size: 108 238 809
Fragments arranged by size:
809 238 108
BglII A'GATC_T
Cuts at: 0 923 1155
Size: 923 232
Bme1580I G_kGCm'C
Cuts at: 0 639 700 761 1155
Size: 639 61 61 394
Fragments arranged by size:
639 394 61 61
BmrI ACTGGGnnnn_n'
Cuts at: 0 633 1155
Size: 633 522
BmtI G_CTAG'C
Cuts at: 0 442 1155
Size: 442 713
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1002 1155
Size: 1002 153
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 790 1155
Size: 790 365
BsaI GGTCTCn'nnnn_
Cuts at: 0 205 1155
Size: 205 950
BsaBI GATnn'nnATC
Cuts at: 0 491 1155
Size: 491 664
BsaHI Gr'CG_yC
Cuts at: 0 914 1155
Size: 914 241
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 994 1155
Size: 994 161
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 126 1155
Size: 126 1029
BsiEI CG_ry'CG
Cuts at: 0 137 1155
Size: 137 1018
BsiHKAI G_wGCw'C
Cuts at: 0 700 761 1155
Size: 700 61 394
Fragments arranged by size:
700 394 61
Bsp1286I G_dGCh'C
Cuts at: 0 639 700 761 1155
Size: 639 61 61 394
Fragments arranged by size:
639 394 61 61
BspHI T'CATG_A
Cuts at: 0 1023 1155
Size: 1023 132
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 451 1155
Size: 451 704
BsrBI CCG'CTC
Cuts at: 0 97 1155
Size: 97 1058
BsrDI GCAATG_nn'
Cuts at: 0 1048 1155
Size: 1048 107
BsrFI r'CCGG_y
Cuts at: 0 81 103 1155
Size: 81 22 1052
Fragments arranged by size:
1052 81 22
BssHII G'CGCG_C
Cuts at: 0 60 1155
Size: 60 1095
BstYI r'GATC_y
Cuts at: 0 486 896 923 1155
Size: 486 410 27 232
Fragments arranged by size:
486 410 232 27
BtgI C'CryG_G
Cuts at: 0 131 890 942 1118 1155
Size: 131 759 52 176 37
Fragments arranged by size:
759 176 131 52 37
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 822 1155
Size: 822 333
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 830 865 1155
Size: 830 35 290
Fragments arranged by size:
830 290 35
EaeI y'GGCC_r
Cuts at: 0 83 122 134 152 255 671 788 935
Size: 83 39 12 18 103 416 117 147
Cuts at: 935 1155
Size: 220
Fragments arranged by size:
416 220 147 117 103 83 39 18
12
EagI C'GGCC_G
Cuts at: 0 134 1155
Size: 134 1021
EarI CTCTTCn'nnn_
Cuts at: 0 406 1155
Size: 406 749
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 358 544 790 1106 1155
Size: 358 186 246 316 49
Fragments arranged by size:
358 316 246 186 49
EcoNI CCTnn'n_nnAGG
Cuts at: 0 351 976 1155
Size: 351 625 179
Fragments arranged by size:
625 351 179
EcoO109I rG'GnC_Cy
Cuts at: 0 635 963 1155
Size: 635 328 192
Fragments arranged by size:
635 328 192
EcoRV GAT'ATC
Cuts at: 0 1068 1155
Size: 1068 87
HaeII r_GCGC'y
Cuts at: 0 174 917 1155
Size: 174 743 238
Fragments arranged by size:
743 238 174
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 645 677 821 853 1155
Size: 645 32 144 32 302
Fragments arranged by size:
645 302 144 32 32
KasI G'GCGC_C
Cuts at: 0 913 1155
Size: 913 242
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 798 807 1155
Size: 798 9 348
Fragments arranged by size:
798 348 9
MscI TGG'CCA
Cuts at: 0 154 257 790 937 1155
Size: 154 103 533 147 218
Fragments arranged by size:
533 218 154 147 103
MslI CAynn'nnrTG
Cuts at: 0 1144 1155
Size: 1144 11
MspA1I CmG'CkG
Cuts at: 0 304 892 1155
Size: 304 588 263
Fragments arranged by size:
588 304 263
NaeI GCC'GGC
Cuts at: 0 83 1155
Size: 83 1072
NarI GG'CG_CC
Cuts at: 0 914 1155
Size: 914 241
NcoI C'CATG_G
Cuts at: 0 1118 1155
Size: 1118 37
NgoMIV G'CCGG_C
Cuts at: 0 81 1155
Size: 81 1074
NheI G'CTAG_C
Cuts at: 0 438 1155
Size: 438 717
PfoI T'CCnGG_A
Cuts at: 0 569 1155
Size: 569 586
PshAI GACnn'nnGTC
Cuts at: 0 719 1155
Size: 719 436
PspOMI G'GGCC_C
Cuts at: 0 635 1155
Size: 635 520
PstI C_TGCA'G
Cuts at: 0 960 1155
Size: 960 195
PvuII CAG'CTG
Cuts at: 0 304 1155
Size: 304 851
SacII CC_GC'GG
Cuts at: 0 893 1155
Size: 893 262
SfcI C'TryA_G
Cuts at: 0 387 812 956 1155
Size: 387 425 144 199
Fragments arranged by size:
425 387 199 144
SfoI GGC'GCC
Cuts at: 0 915 1155
Size: 915 240
StuI AGG'CCT
Cuts at: 0 767 1155
Size: 767 388
StyI C'CwwG_G
Cuts at: 0 174 1118 1155
Size: 174 944 37
Fragments arranged by size:
944 174 37
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 776 955 1155
Size: 776 179 200
Fragments arranged by size:
776 200 179
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 679 711 1155
Size: 679 32 444
Fragments arranged by size:
679 444 32
Tth111I GACn'n_nGTC
Cuts at: 0 659 1155
Size: 659 496
XmnI GAAnn'nnTTC
Cuts at: 0 248 578 1091 1155
Size: 248 330 513 64
Fragments arranged by size:
513 330 248 64
Enzymes that do cut:
AccI AcuI AloI AlwNI ApaI ApaLI ApoI BanI
BanII BbeI BbsI BglII Bme1580I BmrI BmtI BplI
BpmI BsaI BsaBI BsaHI BseRI BsgI BsiEI BsiHKAI
Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BssHII BstYI
BtgI BtgZI CspCI EaeI EagI EarI Eco57MI EcoNI
EcoO109I EcoRV HaeII Hin4I KasI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NheI PfoI PshAI
PspOMI PstI PvuII SacII SfcI SfoI StuI StyI
TaqII TstI Tth111I XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AlfI AscI AseI AsiSI AvaI AvrII
BaeI BamHI BbvCI BcgI BciVI BclI BfrBI BglI
BlpI BmgBI Bpu10I BpuEI BsaAI BsaWI BsaXI BseYI
BsiWI BsmI BsmBI BspEI BsrGI BssSI BstAPI BstBI
BstEII BstXI BstZ17I Bsu36I BtsI ClaI DraI DraIII
DrdI EciI EcoICRI EcoRI FalI FseI FspI FspAI
HincII HindIII HpaI KpnI MfeI MluI NdeI NotI
NruI NsiI NspI PacI PasI PciI PflMI PmeI
PmlI PpiI PpuMI PsiI PspXI PsrI PvuI RsrII
SacI SalI SanDI SapI SbfI ScaI SexAI SfiI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI
SspI SwaI TatI XbaI XcmI XhoI XmaI ZraI