Restriction map for
pFN21AE3163
(Linear) (Six-base) MAPSORT of: KIEE3163.seq Check: 5819 from: 1 to: 1143
KIEE3163.orf
With 182 enzymes: SgfI *
March 22, 2012 16:17 ..
Acc65I G'GTAC_C
Cuts at: 0 1118 1143
Size: 1118 25
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 907 1143
Size: 907 236
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 707 1143
Size: 707 436
AlwNI CAG_nnn'CTG
Cuts at: 0 986 1143
Size: 986 157
ApoI r'AATT_y
Cuts at: 0 153 393 450 795 844 1056 1143
Size: 153 240 57 345 49 212 87
Fragments arranged by size:
345 240 212 153 87 57 49
AvaI C'yCGr_G
Cuts at: 0 30 1143
Size: 30 1113
BamHI G'GATC_C
Cuts at: 0 591 1143
Size: 591 552
BanI G'GyrC_C
Cuts at: 0 1118 1143
Size: 1118 25
BbsI GAAGACnn'nnnn_
Cuts at: 0 452 1143
Size: 452 691
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 567 601 1143
Size: 567 34 542
Fragments arranged by size:
567 542 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 931 1143
Size: 931 212
BfrBI ATG'CAT
Cuts at: 0 620 1143
Size: 620 523
BmgBI CAC'GTC
Cuts at: 0 833 1143
Size: 833 310
BmrI ACTGGGnnnn_n'
Cuts at: 0 442 1143
Size: 442 701
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 202 1143
Size: 202 941
BsaAI yAC'GTr
Cuts at: 0 1089 1143
Size: 1089 54
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 11 41 819 849 1102 1132 1143
Size: 11 30 778 30 253 30 11
Fragments arranged by size:
778 253 30 30 30 11 11
BseYI C'CCAG_C
Cuts at: 0 70 1042 1046 1143
Size: 70 972 4 97
Fragments arranged by size:
972 97 70 4
BsmBI CGTCTCn'nnnn_
Cuts at: 0 839 1143
Size: 839 304
BspHI T'CATG_A
Cuts at: 0 408 1143
Size: 408 735
BsrGI T'GTAC_A
Cuts at: 0 245 1143
Size: 245 898
BssSI C'ACGA_G
Cuts at: 0 839 1143
Size: 839 304
BstAPI GCAn_nnn'nTGC
Cuts at: 0 23 1143
Size: 23 1120
BstEII G'GTnAC_C
Cuts at: 0 550 1143
Size: 550 593
BstYI r'GATC_y
Cuts at: 0 162 591 1143
Size: 162 429 552
Fragments arranged by size:
552 429 162
Bsu36I CC'TnA_GG
Cuts at: 0 527 1143
Size: 527 616
BtsI GCAGTG_nn'
Cuts at: 0 1120 1143
Size: 1120 23
EarI CTCTTCn'nnn_
Cuts at: 0 527 701 1143
Size: 527 174 442
Fragments arranged by size:
527 442 174
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 202 907 1143
Size: 202 705 236
Fragments arranged by size:
705 236 202
EcoO109I rG'GnC_Cy
Cuts at: 0 400 1143
Size: 400 743
EcoRI G'AATT_C
Cuts at: 0 450 1056 1143
Size: 450 606 87
Fragments arranged by size:
606 450 87
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 26 544 576 819 851 1143
Size: 26 518 32 243 32 292
Fragments arranged by size:
518 292 243 32 32 26
KpnI G_GTAC'C
Cuts at: 0 1122 1143
Size: 1122 21
MfeI C'AATT_G
Cuts at: 0 517 999 1143
Size: 517 482 144
Fragments arranged by size:
517 482 144
MslI CAynn'nnrTG
Cuts at: 0 868 1143
Size: 868 275
MspA1I CmG'CkG
Cuts at: 0 82 1046 1143
Size: 82 964 97
Fragments arranged by size:
964 97 82
NsiI A_TGCA'T
Cuts at: 0 622 1143
Size: 622 521
NspI r_CATG'y
Cuts at: 0 715 1143
Size: 715 428
PfoI T'CCnGG_A
Cuts at: 0 218 923 1143
Size: 218 705 220
Fragments arranged by size:
705 220 218
PpuMI rG'GwC_Cy
Cuts at: 0 400 1143
Size: 400 743
PshAI GACnn'nnGTC
Cuts at: 0 416 1143
Size: 416 727
PsiI TTA'TAA
Cuts at: 0 326 1143
Size: 326 817
PstI C_TGCA'G
Cuts at: 0 445 1143
Size: 445 698
PvuII CAG'CTG
Cuts at: 0 82 1046 1143
Size: 82 964 97
Fragments arranged by size:
964 97 82
SapI GCTCTTCn'nnn_
Cuts at: 0 527 701 1143
Size: 527 174 442
Fragments arranged by size:
527 442 174
SfcI C'TryA_G
Cuts at: 0 77 441 1143
Size: 77 364 702
Fragments arranged by size:
702 364 77
SmaI CCC'GGG
Cuts at: 0 32 1143
Size: 32 1111
SnaBI TAC'GTA
Cuts at: 0 1089 1143
Size: 1089 54
SpeI A'CTAG_T
Cuts at: 0 646 1143
Size: 646 497
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 618 1038 1122 1143
Size: 618 420 84 21
Fragments arranged by size:
618 420 84 21
TatI w'GTAC_w
Cuts at: 0 245 1143
Size: 245 898
Tth111I GACn'n_nGTC
Cuts at: 0 831 1143
Size: 831 312
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 949 1143
Size: 949 194
XmaI C'CCGG_G
Cuts at: 0 30 1143
Size: 30 1113
Enzymes that do cut:
Acc65I AcuI AlfI AlwNI ApoI AvaI BamHI BanI
BbsI BcgI BciVI BfrBI BmgBI BmrI BpmI BsaAI
BsaXI BseYI BsmBI BspHI BsrGI BssSI BstAPI BstEII
BstYI Bsu36I BtsI EarI Eco57MI EcoO109I EcoRI Hin4I
KpnI MfeI MslI MspA1I NsiI NspI PfoI PpuMI
PshAI PsiI PstI PvuII SapI SfcI SmaI SnaBI
SpeI TaqII TatI Tth111I XcmI XmaI
Enzymes that do not cut:
AarI AatII AccI AclI AfeI AflII AflIII AgeI
AhdI AleI AloI ApaI ApaLI AscI AseI AsiSI
AvrII BaeI BanII BbeI BbvCI BclI BglI BglII
BlpI Bme1580I BmtI BplI Bpu10I BpuEI BsaI BsaBI
BsaHI BsaWI BseRI BsgI BsiEI BsiHKAI BsiWI BsmI
Bsp1286I BspEI BspMI BsrBI BsrDI BsrFI BssHII BstBI
BstXI BstZ17I BtgI BtgZI ClaI CspCI DraI DraIII
DrdI EaeI EagI EciI EcoICRI EcoNI EcoRV FalI
FseI FspI FspAI HaeII HincII HindIII HpaI KasI
MluI MmeI MscI NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI PacI PasI PciI PflMI PmeI
PmlI PpiI PspOMI PspXI PsrI PvuI RsrII SacI
SacII SalI SanDI SbfI ScaI SexAI SfiI SfoI
SgfI SgrAI SmlI SphI SrfI SspI StuI StyI
SwaI TstI XbaI XhoI XmnI ZraI