Restriction map for
pFN21AE3160
(Linear) (Six-base) MAPSORT of: KIEE3160.seq Check: 7256 from: 1 to: 1140
KIEE3160.orf
With 182 enzymes: SgfI *
December 27, 2011 16:33 ..
AccI GT'mk_AC
Cuts at: 0 731 1140
Size: 731 409
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 18 104 1140
Size: 18 86 1036
Fragments arranged by size:
1036 86 18
AleI CACnn'nnGTG
Cuts at: 0 877 1140
Size: 877 263
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 895 1140
Size: 895 245
AlwNI CAG_nnn'CTG
Cuts at: 0 11 678 759 912 1140
Size: 11 667 81 153 228
Fragments arranged by size:
667 228 153 81 11
ApaLI G'TGCA_C
Cuts at: 0 432 1140
Size: 432 708
AvaI C'yCGr_G
Cuts at: 0 98 292 1001 1140
Size: 98 194 709 139
Fragments arranged by size:
709 194 139 98
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 967 1000 1140
Size: 967 33 140
Fragments arranged by size:
967 140 33
BanI G'GyrC_C
Cuts at: 0 384 1140
Size: 384 756
BanII G_rGCy'C
Cuts at: 0 299 650 868 1140
Size: 299 351 218 272
Fragments arranged by size:
351 299 272 218
BbsI GAAGACnn'nnnn_
Cuts at: 0 92 1140
Size: 92 1048
BciVI GTATCCnnnnn_n'
Cuts at: 0 995 1140
Size: 995 145
BfrBI ATG'CAT
Cuts at: 0 26 1140
Size: 26 1114
BglII A'GATC_T
Cuts at: 0 1058 1140
Size: 1058 82
Bme1580I G_kGCm'C
Cuts at: 0 389 436 818 1140
Size: 389 47 382 322
Fragments arranged by size:
389 382 322 47
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 427 700 1140
Size: 427 273 440
Fragments arranged by size:
440 427 273
Bpu10I CC'TnA_GC
Cuts at: 0 862 1140
Size: 862 278
BsaI GGTCTCn'nnnn_
Cuts at: 0 878 1140
Size: 878 262
BsaAI yAC'GTr
Cuts at: 0 801 1140
Size: 801 339
BsaWI w'CCGG_w
Cuts at: 0 498 1140
Size: 498 642
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 79 109 1140
Size: 79 30 1031
Fragments arranged by size:
1031 79 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 232 379 424 1140
Size: 232 147 45 716
Fragments arranged by size:
716 232 147 45
BseYI C'CCAG_C
Cuts at: 0 54 905 1140
Size: 54 851 235
Fragments arranged by size:
851 235 54
BsiHKAI G_wGCw'C
Cuts at: 0 436 650 1140
Size: 436 214 490
Fragments arranged by size:
490 436 214
BsmI GAATG_Cn'
Cuts at: 0 28 1140
Size: 28 1112
Bsp1286I G_dGCh'C
Cuts at: 0 299 389 436 650 818 868 1140
Size: 299 90 47 214 168 50 272
Fragments arranged by size:
299 272 214 168 90 50 47
BspHI T'CATG_A
Cuts at: 0 392 1140
Size: 392 748
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 292 686 1140
Size: 292 394 454
Fragments arranged by size:
454 394 292
BsrBI CCG'CTC
Cuts at: 0 1101 1140
Size: 1101 39
BsrDI GCAATG_nn'
Cuts at: 0 240 662 1140
Size: 240 422 478
Fragments arranged by size:
478 422 240
BsrFI r'CCGG_y
Cuts at: 0 428 1140
Size: 428 712
BsrGI T'GTAC_A
Cuts at: 0 125 1140
Size: 125 1015
BstAPI GCAn_nnn'nTGC
Cuts at: 0 11 23 1140
Size: 11 12 1117
Fragments arranged by size:
1117 12 11
BstXI CCAn_nnnn'nTGG
Cuts at: 0 405 1140
Size: 405 735
BstYI r'GATC_y
Cuts at: 0 1058 1140
Size: 1058 82
BstZ17I GTA'TAC
Cuts at: 0 732 1140
Size: 732 408
Bsu36I CC'TnA_GG
Cuts at: 0 423 1140
Size: 423 717
BtgI C'CryG_G
Cuts at: 0 359 1140
Size: 359 781
DrdI GACnn_nn'nnGTC
Cuts at: 0 705 1140
Size: 705 435
EaeI y'GGCC_r
Cuts at: 0 487 894 1140
Size: 487 407 246
Fragments arranged by size:
487 407 246
EarI CTCTTCn'nnn_
Cuts at: 0 250 1001 1140
Size: 250 751 139
Fragments arranged by size:
751 250 139
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 598 1140
Size: 598 542
EcoICRI GAG'CTC
Cuts at: 0 648 1140
Size: 648 492
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 18 104 427 700 1140
Size: 18 86 323 273 440
Fragments arranged by size:
440 323 273 86 18
EcoNI CCTnn'n_nnAGG
Cuts at: 0 781 1140
Size: 781 359
EcoO109I rG'GnC_Cy
Cuts at: 0 58 78 567 756 1140
Size: 58 20 489 189 384
Fragments arranged by size:
489 384 189 58 20
FspI TGC'GCA
Cuts at: 0 661 1140
Size: 661 479
FspAI rTGC'GCAy
Cuts at: 0 661 1140
Size: 661 479
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 50 57 79 82 89 111 183 215
Size: 50 7 22 3 7 22 72 32
Cuts at: 215 757 789 813 845 1140
Size: 542 32 24 32 295
Fragments arranged by size:
542 295 72 50 32 32 32 24
22 22 7 7 3
HindIII A'AGCT_T
Cuts at: 0 157 1140
Size: 157 983
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 479 1140
Size: 479 661
MslI CAynn'nnrTG
Cuts at: 0 175 877 923 1049 1140
Size: 175 702 46 126 91
Fragments arranged by size:
702 175 126 91 46
MspA1I CmG'CkG
Cuts at: 0 8 486 912 1140
Size: 8 478 426 228
Fragments arranged by size:
478 426 228 8
NcoI C'CATG_G
Cuts at: 0 359 1140
Size: 359 781
NsiI A_TGCA'T
Cuts at: 0 28 1140
Size: 28 1112
NspI r_CATG'y
Cuts at: 0 791 922 1140
Size: 791 131 218
Fragments arranged by size:
791 218 131
PflMI CCAn_nnn'nTGG
Cuts at: 0 759 1092 1140
Size: 759 333 48
Fragments arranged by size:
759 333 48
PfoI T'CCnGG_A
Cuts at: 0 752 833 1140
Size: 752 81 307
Fragments arranged by size:
752 307 81
PpuMI rG'GwC_Cy
Cuts at: 0 58 78 567 756 1140
Size: 58 20 489 189 384
Fragments arranged by size:
489 384 189 58 20
PspXI vC'TCGA_Gb
Cuts at: 0 292 1140
Size: 292 848
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 667 699 1140
Size: 667 32 441
Fragments arranged by size:
667 441 32
PstI C_TGCA'G
Cuts at: 0 152 859 917 1140
Size: 152 707 58 223
Fragments arranged by size:
707 223 152 58
PvuII CAG'CTG
Cuts at: 0 912 1140
Size: 912 228
SacI G_AGCT'C
Cuts at: 0 650 1140
Size: 650 490
SanDI GG'GwC_CC
Cuts at: 0 58 1140
Size: 58 1082
SapI GCTCTTCn'nnn_
Cuts at: 0 1001 1140
Size: 1001 139
ScaI AGT'ACT
Cuts at: 0 721 1140
Size: 721 419
SexAI A'CCwGG_T
Cuts at: 0 653 758 1140
Size: 653 105 382
Fragments arranged by size:
653 382 105
SfcI C'TryA_G
Cuts at: 0 148 855 913 976 1140
Size: 148 707 58 63 164
Fragments arranged by size:
707 164 148 63 58
SmlI C'TyrA_G
Cuts at: 0 292 1140
Size: 292 848
StyI C'CwwG_G
Cuts at: 0 359 746 1140
Size: 359 387 394
Fragments arranged by size:
394 387 359
TatI w'GTAC_w
Cuts at: 0 125 224 683 719 1140
Size: 125 99 459 36 421
Fragments arranged by size:
459 421 125 99 36
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 569 601 1140
Size: 569 32 539
Fragments arranged by size:
569 539 32
Tth111I GACn'n_nGTC
Cuts at: 0 115 1140
Size: 115 1025
XhoI C'TCGA_G
Cuts at: 0 292 1140
Size: 292 848
Enzymes that do cut:
AccI AcuI AleI AlfI AlwNI ApaLI AvaI BaeI
BanI BanII BbsI BciVI BfrBI BglII Bme1580I BpmI
Bpu10I BsaI BsaAI BsaWI BsaXI BseRI BseYI BsiHKAI
BsmI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BstAPI BstXI BstYI BstZ17I Bsu36I BtgI DrdI EaeI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I FspI FspAI
Hin4I HindIII MmeI MslI MspA1I NcoI NsiI NspI
PflMI PfoI PpuMI PspXI PsrI PstI PvuII SacI
SanDI SapI ScaI SexAI SfcI SmlI StyI TatI
TstI Tth111I XhoI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AhdI AloI ApaI ApoI AscI AseI AsiSI AvrII
BamHI BbeI BbvCI BcgI BclI BglI BlpI BmgBI
BmrI BmtI BplI BpuEI BsaBI BsaHI BsgI BsiEI
BsiWI BsmBI BspEI BssHII BssSI BstBI BstEII BtgZI
BtsI ClaI CspCI DraI DraIII EagI EcoRI EcoRV
FalI FseI HaeII HincII HpaI KasI KpnI MfeI
MluI MscI NaeI NarI NdeI NgoMIV NheI NotI
NruI PacI PasI PciI PmeI PmlI PpiI PshAI
PsiI PspOMI PvuI RsrII SacII SalI SbfI SfiI
SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI
SspI StuI SwaI TaqII XbaI XcmI XmaI XmnI
ZraI