Restriction map for
pFN21AE3159
(Linear) (Six-base) MAPSORT of: KIEE3159.seq Check: 580 from: 1 to: 1134
KIEE3159.orf
With 182 enzymes: SgfI *
December 27, 2011 16:33 ..
Acc65I G'GTAC_C
Cuts at: 0 1006 1134
Size: 1006 128
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 383 470 1134
Size: 383 87 664
Fragments arranged by size:
664 383 87
AflIII A'CryG_T
Cuts at: 0 137 1134
Size: 137 997
AlwNI CAG_nnn'CTG
Cuts at: 0 511 1134
Size: 511 623
ApoI r'AATT_y
Cuts at: 0 18 328 338 984 1134
Size: 18 310 10 646 150
Fragments arranged by size:
646 310 150 18 10
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 51 84 1134
Size: 51 33 1050
Fragments arranged by size:
1050 51 33
BamHI G'GATC_C
Cuts at: 0 806 1134
Size: 806 328
BanI G'GyrC_C
Cuts at: 0 48 1006 1134
Size: 48 958 128
Fragments arranged by size:
958 128 48
BanII G_rGCy'C
Cuts at: 0 713 1134
Size: 713 421
BciVI GTATCCnnnnn_n'
Cuts at: 0 590 1134
Size: 590 544
BclI T'GATC_A
Cuts at: 0 351 1134
Size: 351 783
BmrI ACTGGGnnnn_n'
Cuts at: 0 73 273 738 1134
Size: 73 200 465 396
Fragments arranged by size:
465 396 200 73
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 647 719 1134
Size: 647 72 415
Fragments arranged by size:
647 415 72
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 408 1134
Size: 408 726
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1013 1134
Size: 1013 121
BseYI C'CCAG_C
Cuts at: 0 114 1134
Size: 114 1020
BsiHKAI G_wGCw'C
Cuts at: 0 713 839 1134
Size: 713 126 295
Fragments arranged by size:
713 295 126
BsmI GAATG_Cn'
Cuts at: 0 434 914 1134
Size: 434 480 220
Fragments arranged by size:
480 434 220
Bsp1286I G_dGCh'C
Cuts at: 0 713 839 1134
Size: 713 126 295
Fragments arranged by size:
713 295 126
BspHI T'CATG_A
Cuts at: 0 762 1134
Size: 762 372
BsrDI GCAATG_nn'
Cuts at: 0 588 1134
Size: 588 546
BstEII G'GTnAC_C
Cuts at: 0 70 1134
Size: 70 1064
BstYI r'GATC_y
Cuts at: 0 806 1134
Size: 806 328
BtsI GCAGTG_nn'
Cuts at: 0 160 1134
Size: 160 974
DraI TTT'AAA
Cuts at: 0 960 1134
Size: 960 174
DraIII CAC_nnn'GTG
Cuts at: 0 81 1134
Size: 81 1053
EarI CTCTTCn'nnn_
Cuts at: 0 221 359 846 1134
Size: 221 138 487 288
Fragments arranged by size:
487 288 221 138
EcoICRI GAG'CTC
Cuts at: 0 711 1134
Size: 711 423
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 383 470 647 719 1134
Size: 383 87 177 72 415
Fragments arranged by size:
415 383 177 87 72
EcoO109I rG'GnC_Cy
Cuts at: 0 550 1134
Size: 550 584
KpnI G_GTAC'C
Cuts at: 0 1010 1134
Size: 1010 124
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 545 1134
Size: 545 589
MspA1I CmG'CkG
Cuts at: 0 208 1134
Size: 208 926
NspI r_CATG'y
Cuts at: 0 141 153 1134
Size: 141 12 981
Fragments arranged by size:
981 141 12
PciI A'CATG_T
Cuts at: 0 137 1134
Size: 137 997
PflMI CCAn_nnn'nTGG
Cuts at: 0 81 1134
Size: 81 1053
PfoI T'CCnGG_A
Cuts at: 0 809 1134
Size: 809 325
PpuMI rG'GwC_Cy
Cuts at: 0 550 1134
Size: 550 584
PvuII CAG'CTG
Cuts at: 0 208 1134
Size: 208 926
SacI G_AGCT'C
Cuts at: 0 713 1134
Size: 713 421
SapI GCTCTTCn'nnn_
Cuts at: 0 221 1134
Size: 221 913
SfcI C'TryA_G
Cuts at: 0 732 1134
Size: 732 402
SmlI C'TyrA_G
Cuts at: 0 423 1134
Size: 423 711
SpeI A'CTAG_T
Cuts at: 0 403 1134
Size: 403 731
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 92 1134
Size: 92 1042
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 614 646 852 884 1134
Size: 614 32 206 32 250
Fragments arranged by size:
614 250 206 32 32
XmnI GAAnn'nnTTC
Cuts at: 0 341 1134
Size: 341 793
Enzymes that do cut:
Acc65I AcuI AflIII AlwNI ApoI BaeI BamHI BanI
BanII BciVI BclI BmrI BpmI BpuEI BseRI BseYI
BsiHKAI BsmI Bsp1286I BspHI BsrDI BstEII BstYI BtsI
DraI DraIII EarI EcoICRI Eco57MI EcoO109I KpnI MmeI
MspA1I NspI PciI PflMI PfoI PpuMI PvuII SacI
SapI SfcI SmlI SpeI TaqII TstI XmnI
Enzymes that do not cut:
AarI AatII AccI AclI AfeI AflII AgeI AhdI
AleI AlfI AloI ApaI ApaLI AscI AseI AsiSI
AvaI AvrII BbeI BbsI BbvCI BcgI BfrBI BglI
BglII BlpI Bme1580I BmgBI BmtI BplI Bpu10I BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BsgI BsiEI BsiWI
BsmBI BspEI BspMI BsrBI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstXI BstZ17I Bsu36I BtgI BtgZI ClaI
CspCI DrdI EaeI EagI EciI EcoNI EcoRI EcoRV
FalI FseI FspI FspAI HaeII Hin4I HincII HindIII
HpaI KasI MfeI MluI MscI MslI NaeI NarI
NcoI NdeI NgoMIV NheI NotI NruI NsiI PacI
PasI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI
PsrI PstI PvuI RsrII SacII SalI SanDI SbfI
ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI
SphI SrfI SspI StuI StyI SwaI TatI Tth111I
XbaI XcmI XhoI XmaI ZraI