(Linear) (Six-base) MAPSORT of: KIEE3158.seq  Check: 7597  from: 1  to: 1128



KIEE3158.orf

 With 182 enzymes: SgfI * 

                        March 22, 2012 16:17  ..

AclI AA'CG_TT

Cuts at:      0     392    1128 
   Size:        392     736
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     673    1128 
   Size:        673     455
 
AlwNI CAG_nnn'CTG

Cuts at:      0     403    1128 
   Size:        403     725
 
ApoI r'AATT_y

Cuts at:      0     485    1128 
   Size:        485     643
 
BclI T'GATC_A

Cuts at:      0     380    1128 
   Size:        380     748
 
BfrBI ATG'CAT

Cuts at:      0       3     253    1128 
   Size:          3     250     875

  Fragments arranged by size: 

                 875     250       3
 
BglII A'GATC_T

Cuts at:      0     684    1128 
   Size:        684     444
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     197    1128 
   Size:        197     931
 
Bpu10I CC'TnA_GC

Cuts at:      0     603    1128 
   Size:        603     525
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     289     579    1128 
   Size:        289     290     549

  Fragments arranged by size: 

                 549     290     289
 
BsaBI GATnn'nnATC

Cuts at:      0    1115    1128 
   Size:       1115      13
 
BsaWI w'CCGG_w

Cuts at:      0     659    1128 
   Size:        659     469
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     209     239    1128 
   Size:        209      30     889

  Fragments arranged by size: 

                 889     209      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1015    1072    1128 
   Size:       1015      57      56

  Fragments arranged by size: 

                1015      57      56
 
BstYI r'GATC_y

Cuts at:      0     684    1128 
   Size:        684     444
 
BtgI C'CryG_G

Cuts at:      0     524     867    1128 
   Size:        524     343     261

  Fragments arranged by size: 

                 524     343     261
 
BtsI GCAGTG_nn'

Cuts at:      0      35    1128 
   Size:         35    1093
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     823     858    1128 
   Size:        823      35     270

  Fragments arranged by size: 

                 823     270      35
 
DraIII CAC_nnn'GTG

Cuts at:      0     994    1128 
   Size:        994     134
 
EarI CTCTTCn'nnn_

Cuts at:      0      48     199     992    1128 
   Size:         48     151     793     136

  Fragments arranged by size: 

                 793     151     136      48
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     197     673    1128 
   Size:        197     476     455

  Fragments arranged by size: 

                 476     455     197
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     215    1128 
   Size:        215     913
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     368     400    1128 
   Size:        368      32     728

  Fragments arranged by size: 

                 728     368      32
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     735    1128 
   Size:        735     393
 
MslI CAynn'nnrTG

Cuts at:      0     556    1128 
   Size:        556     572
 
NcoI C'CATG_G

Cuts at:      0     867    1128 
   Size:        867     261
 
NsiI A_TGCA'T

Cuts at:      0       5     255    1128 
   Size:          5     250     873

  Fragments arranged by size: 

                 873     250       5
 
NspI r_CATG'y

Cuts at:      0     257     878    1128 
   Size:        257     621     250

  Fragments arranged by size: 

                 621     257     250
 
PacI TTA_AT'TAA

Cuts at:      0     500    1128 
   Size:        500     628
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     403    1128 
   Size:        403     725
 
PshAI GACnn'nnGTC

Cuts at:      0     178    1128 
   Size:        178     950
 
PstI C_TGCA'G

Cuts at:      0    1029    1128 
   Size:       1029      99
 
SbfI CC_TGCA'GG

Cuts at:      0    1029    1128 
   Size:       1029      99
 
ScaI AGT'ACT

Cuts at:      0     289    1128 
   Size:        289     839
 
SexAI A'CCwGG_T

Cuts at:      0     410    1128 
   Size:        410     718
 
SfcI C'TryA_G

Cuts at:      0    1025    1128 
   Size:       1025     103
 
SmlI C'TyrA_G

Cuts at:      0      10    1128 
   Size:         10    1118
 
StyI C'CwwG_G

Cuts at:      0     867    1128 
   Size:        867     261
 
TatI w'GTAC_w

Cuts at:      0     287    1128 
   Size:        287     841
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     209     241    1128 
   Size:        209      32     887

  Fragments arranged by size: 

                 887     209      32
 
XmnI GAAnn'nnTTC

Cuts at:      0    1112    1128 
   Size:       1112      16
 

 Enzymes that do cut:

     AclI     AcuI    AlwNI     ApoI     BclI    BfrBI    BglII     BpmI
   Bpu10I     BsaI    BsaBI    BsaWI    BsaXI    BseRI    BstYI     BtgI
     BtsI    CspCI   DraIII     EarI  Eco57MI     FalI    Hin4I     MmeI
     MslI     NcoI     NsiI     NspI     PacI    PflMI    PshAI     PstI
     SbfI     ScaI    SexAI     SfcI     SmlI     StyI     TatI     TstI
     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI    BanII     BbeI
     BbsI    BbvCI     BcgI    BciVI     BglI     BlpI Bme1580I    BmgBI
     BmrI     BmtI     BplI    BpuEI    BsaAI    BsaHI    BseYI     BsgI
    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI Bsp1286I    BspEI    BspHI
    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI
    BstBI   BstEII    BstXI  BstZ17I   Bsu36I    BtgZI     ClaI     DraI
     DrdI     EaeI     EagI     EciI  EcoICRI    EcoNI EcoO109I    EcoRI
    EcoRV     FseI     FspI    FspAI    HaeII   HincII  HindIII     HpaI
     KasI     KpnI     MfeI     MluI     MscI   MspA1I     NaeI     NarI
     NdeI   NgoMIV     NheI     NotI     NruI     PasI     PciI     PfoI
     PmeI     PmlI     PpiI    PpuMI     PsiI   PspOMI    PspXI     PsrI
     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI     SapI
     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI
     SrfI     SspI     StuI     SwaI    TaqII  Tth111I     XbaI     XcmI
     XhoI     XmaI     ZraI