Restriction map for
pFN21AE3157
(Linear) (Six-base) MAPSORT of: KIEE3157.seq Check: 1415 from: 1 to: 1125
KIEE3157.orf
With 182 enzymes: SgfI *
December 27, 2011 16:33 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 83 553 1125
Size: 83 470 572
Fragments arranged by size:
572 470 83
AfeI AGC'GCT
Cuts at: 0 754 1125
Size: 754 371
AleI CACnn'nnGTG
Cuts at: 0 644 1125
Size: 644 481
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 773 878 1125
Size: 773 105 247
Fragments arranged by size:
773 247 105
AlwNI CAG_nnn'CTG
Cuts at: 0 600 798 1125
Size: 600 198 327
Fragments arranged by size:
600 327 198
ApaI G_GGCC'C
Cuts at: 0 315 1125
Size: 315 810
ApaLI G'TGCA_C
Cuts at: 0 397 1125
Size: 397 728
AvaI C'yCGr_G
Cuts at: 0 359 1037 1125
Size: 359 678 88
Fragments arranged by size:
678 359 88
BanI G'GyrC_C
Cuts at: 0 274 608 846 1125
Size: 274 334 238 279
Fragments arranged by size:
334 279 274 238
BanII G_rGCy'C
Cuts at: 0 315 505 572 773 1125
Size: 315 190 67 201 352
Fragments arranged by size:
352 315 201 190 67
BbeI G_GCGC'C
Cuts at: 0 612 850 1125
Size: 612 238 275
Fragments arranged by size:
612 275 238
BbsI GAAGACnn'nnnn_
Cuts at: 0 538 1105 1125
Size: 538 567 20
Fragments arranged by size:
567 538 20
BciVI GTATCCnnnnn_n'
Cuts at: 0 229 1125
Size: 229 896
BfrBI ATG'CAT
Cuts at: 0 855 1125
Size: 855 270
BglI GCCn_nnn'nGGC
Cuts at: 0 668 1125
Size: 668 457
BlpI GC'TnA_GC
Cuts at: 0 499 761 1125
Size: 499 262 364
Fragments arranged by size:
499 364 262
Bme1580I G_kGCm'C
Cuts at: 0 277 303 315 401 1125
Size: 277 26 12 86 724
Fragments arranged by size:
724 277 86 26 12
BmgBI CAC'GTC
Cuts at: 0 264 402 735 1125
Size: 264 138 333 390
Fragments arranged by size:
390 333 264 138
BmrI ACTGGGnnnn_n'
Cuts at: 0 240 1125
Size: 240 885
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 168 1125
Size: 168 957
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 118 406 642 685 820 911 1125
Size: 118 288 236 43 135 91 214
Fragments arranged by size:
288 236 214 135 118 91 43
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 46 1125
Size: 46 1079
BsaI GGTCTCn'nnnn_
Cuts at: 0 1079 1125
Size: 1079 46
BsaHI Gr'CG_yC
Cuts at: 0 609 847 1125
Size: 609 238 278
Fragments arranged by size:
609 278 238
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 947 977 1125
Size: 947 30 148
Fragments arranged by size:
947 148 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 187 955 1108 1125
Size: 187 768 153 17
Fragments arranged by size:
768 187 153 17
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 469 733 1125
Size: 469 264 392
Fragments arranged by size:
469 392 264
BsiEI CG_ry'CG
Cuts at: 0 748 1125
Size: 748 377
BsiHKAI G_wGCw'C
Cuts at: 0 401 505 572 773 1125
Size: 401 104 67 201 352
Fragments arranged by size:
401 352 201 104 67
BsmI GAATG_Cn'
Cuts at: 0 281 1125
Size: 281 844
Bsp1286I G_dGCh'C
Cuts at: 0 277 303 315 401 505 572 773 1125
Size: 277 26 12 86 104 67 201 352
Fragments arranged by size:
352 277 201 104 86 67 26 12
BsrBI CCG'CTC
Cuts at: 0 84 1125
Size: 84 1041
BsrFI r'CCGG_y
Cuts at: 0 277 555 781 981 1125
Size: 277 278 226 200 144
Fragments arranged by size:
278 277 226 200 144
BssSI C'ACGA_G
Cuts at: 0 169 487 1125
Size: 169 318 638
Fragments arranged by size:
638 318 169
BstAPI GCAn_nnn'nTGC
Cuts at: 0 405 1125
Size: 405 720
BstEII G'GTnAC_C
Cuts at: 0 879 1125
Size: 879 246
BtgI C'CryG_G
Cuts at: 0 1115 1125
Size: 1115 10
BtsI GCAGTG_nn'
Cuts at: 0 238 1125
Size: 238 887
DrdI GACnn_nn'nnGTC
Cuts at: 0 163 1125
Size: 163 962
EaeI y'GGCC_r
Cuts at: 0 745 941 1125
Size: 745 196 184
Fragments arranged by size:
745 196 184
EagI C'GGCC_G
Cuts at: 0 745 1125
Size: 745 380
EarI CTCTTCn'nnn_
Cuts at: 0 59 577 1125
Size: 59 518 548
Fragments arranged by size:
548 518 59
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 552 1125
Size: 552 573
EcoICRI GAG'CTC
Cuts at: 0 503 570 771 1125
Size: 503 67 201 354
Fragments arranged by size:
503 354 201 67
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 83 118 406 553 642 685 820 911
Size: 83 35 288 147 89 43 135 91
Cuts at: 911 1125
Size: 214
Fragments arranged by size:
288 214 147 135 91 89 83 43
35
EcoO109I rG'GnC_Cy
Cuts at: 0 311 1125
Size: 311 814
HaeII r_GCGC'y
Cuts at: 0 344 612 756 822 850 964 1125
Size: 344 268 144 66 28 114 161
Fragments arranged by size:
344 268 161 144 114 66 28
HindIII A'AGCT_T
Cuts at: 0 22 1125
Size: 22 1103
KasI G'GCGC_C
Cuts at: 0 608 846 1125
Size: 608 238 279
Fragments arranged by size:
608 279 238
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 881 1125
Size: 881 244
MslI CAynn'nnrTG
Cuts at: 0 644 1125
Size: 644 481
MspA1I CmG'CkG
Cuts at: 0 135 827 833 1125
Size: 135 692 6 292
Fragments arranged by size:
692 292 135 6
NaeI GCC'GGC
Cuts at: 0 557 783 1125
Size: 557 226 342
Fragments arranged by size:
557 342 226
NarI GG'CG_CC
Cuts at: 0 609 847 1125
Size: 609 238 278
Fragments arranged by size:
609 278 238
NcoI C'CATG_G
Cuts at: 0 1115 1125
Size: 1115 10
NgoMIV G'CCGG_C
Cuts at: 0 555 781 1125
Size: 555 226 344
Fragments arranged by size:
555 344 226
NsiI A_TGCA'T
Cuts at: 0 857 1125
Size: 857 268
PflMI CCAn_nnn'nTGG
Cuts at: 0 600 950 1125
Size: 600 350 175
Fragments arranged by size:
600 350 175
PspOMI G'GGCC_C
Cuts at: 0 311 1125
Size: 311 814
PstI C_TGCA'G
Cuts at: 0 832 1125
Size: 832 293
PvuII CAG'CTG
Cuts at: 0 135 833 1125
Size: 135 698 292
Fragments arranged by size:
698 292 135
RsrII CG'GwC_CG
Cuts at: 0 186 1125
Size: 186 939
SacI G_AGCT'C
Cuts at: 0 505 572 773 1125
Size: 505 67 201 352
Fragments arranged by size:
505 352 201 67
SapI GCTCTTCn'nnn_
Cuts at: 0 577 1125
Size: 577 548
SfcI C'TryA_G
Cuts at: 0 425 828 1125
Size: 425 403 297
Fragments arranged by size:
425 403 297
SfoI GGC'GCC
Cuts at: 0 610 848 1125
Size: 610 238 277
Fragments arranged by size:
610 277 238
SmaI CCC'GGG
Cuts at: 0 361 1039 1125
Size: 361 678 86
Fragments arranged by size:
678 361 86
SmlI C'TyrA_G
Cuts at: 0 25 1125
Size: 25 1100
StyI C'CwwG_G
Cuts at: 0 1115 1125
Size: 1115 10
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 153 1125
Size: 153 972
TatI w'GTAC_w
Cuts at: 0 137 1125
Size: 137 988
Tth111I GACn'n_nGTC
Cuts at: 0 190 1125
Size: 190 935
XmaI C'CCGG_G
Cuts at: 0 359 1037 1125
Size: 359 678 88
Fragments arranged by size:
678 359 88
Enzymes that do cut:
AcuI AfeI AleI AlfI AlwNI ApaI ApaLI AvaI
BanI BanII BbeI BbsI BciVI BfrBI BglI BlpI
Bme1580I BmgBI BmrI BplI BpmI BpuEI BsaI BsaHI
BsaXI BseRI BsgI BsiEI BsiHKAI BsmI Bsp1286I BsrBI
BsrFI BssSI BstAPI BstEII BtgI BtsI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I HaeII HindIII
KasI MmeI MslI MspA1I NaeI NarI NcoI NgoMIV
NsiI PflMI PspOMI PstI PvuII RsrII SacI SapI
SfcI SfoI SmaI SmlI StyI TaqII TatI Tth111I
XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AflII AflIII AgeI
AhdI AloI ApoI AscI AseI AsiSI AvrII BaeI
BamHI BbvCI BcgI BclI BglII BmtI Bpu10I BsaAI
BsaBI BsaWI BseYI BsiWI BsmBI BspEI BspHI BspMI
BsrDI BsrGI BssHII BstBI BstXI BstYI BstZ17I Bsu36I
BtgZI ClaI CspCI DraI DraIII EcoNI EcoRI EcoRV
FalI FseI FspI FspAI Hin4I HincII HpaI KpnI
MfeI MluI MscI NdeI NheI NotI NruI NspI
PacI PasI PciI PfoI PmeI PmlI PpiI PpuMI
PshAI PsiI PspXI PsrI PvuI SacII SalI SanDI
SbfI ScaI SexAI SfiI SgfI SgrAI SnaBI SpeI
SphI SrfI SspI StuI SwaI TstI XbaI XcmI
XhoI XmnI ZraI