Restriction map for
pFN21AE3156
(Linear) (Six-base) MAPSORT of: KIEE3156.seq Check: 9716 from: 1 to: 1125
KIEE3156.orf
With 182 enzymes: SgfI *
December 27, 2011 16:33 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 49 242 395 464 599 751 1125
Size: 49 193 153 69 135 152 374
Fragments arranged by size:
374 193 153 152 135 69 49
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 995 1125
Size: 995 130
AlwNI CAG_nnn'CTG
Cuts at: 0 114 598 1125
Size: 114 484 527
Fragments arranged by size:
527 484 114
ApoI r'AATT_y
Cuts at: 0 534 1125
Size: 534 591
BanI G'GyrC_C
Cuts at: 0 117 1125
Size: 117 1008
BanII G_rGCy'C
Cuts at: 0 17 638 1053 1125
Size: 17 621 415 72
Fragments arranged by size:
621 415 72 17
BbsI GAAGACnn'nnnn_
Cuts at: 0 230 461 568 1125
Size: 230 231 107 557
Fragments arranged by size:
557 231 230 107
BglII A'GATC_T
Cuts at: 0 770 1125
Size: 770 355
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 957 1125
Size: 957 168
Bpu10I CC'TnA_GC
Cuts at: 0 338 637 1125
Size: 338 299 488
Fragments arranged by size:
488 338 299
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1 1125
Size: 1 1124
BsaHI Gr'CG_yC
Cuts at: 0 40 1125
Size: 40 1085
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 146 176 872 902 1125
Size: 146 30 696 30 223
Fragments arranged by size:
696 223 146 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 914 1125
Size: 914 211
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 85 611 1030 1125
Size: 85 526 419 95
Fragments arranged by size:
526 419 95 85
BsiHKAI G_wGCw'C
Cuts at: 0 638 1125
Size: 638 487
Bsp1286I G_dGCh'C
Cuts at: 0 17 638 1053 1125
Size: 17 621 415 72
Fragments arranged by size:
621 415 72 17
BsrGI T'GTAC_A
Cuts at: 0 1074 1125
Size: 1074 51
BssSI C'ACGA_G
Cuts at: 0 61 1125
Size: 61 1064
BstAPI GCAn_nnn'nTGC
Cuts at: 0 144 1125
Size: 144 981
BstXI CCAn_nnnn'nTGG
Cuts at: 0 586 1125
Size: 586 539
BstYI r'GATC_y
Cuts at: 0 770 1125
Size: 770 355
BtgI C'CryG_G
Cuts at: 0 377 1125
Size: 377 748
BtsI GCAGTG_nn'
Cuts at: 0 102 165 599 948 1125
Size: 102 63 434 349 177
Fragments arranged by size:
434 349 177 102 63
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 280 315 1125
Size: 280 35 810
Fragments arranged by size:
810 280 35
DraI TTT'AAA
Cuts at: 0 584 1125
Size: 584 541
EarI CTCTTCn'nnn_
Cuts at: 0 413 428 479 1125
Size: 413 15 51 646
Fragments arranged by size:
646 413 51 15
EcoICRI GAG'CTC
Cuts at: 0 636 1125
Size: 636 489
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 49 242 395 464 599 751 957 1125
Size: 49 193 153 69 135 152 206 168
Fragments arranged by size:
206 193 168 153 152 135 69 49
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 846 1125
Size: 846 279
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 59 91 348 380 1125
Size: 59 32 257 32 745
Fragments arranged by size:
745 257 59 32 32
HincII GTy'rAC
Cuts at: 0 738 1125
Size: 738 387
HindIII A'AGCT_T
Cuts at: 0 611 1125
Size: 611 514
MfeI C'AATT_G
Cuts at: 0 292 1125
Size: 292 833
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 64 1125
Size: 64 1061
MslI CAynn'nnrTG
Cuts at: 0 204 347 584 1125
Size: 204 143 237 541
Fragments arranged by size:
541 237 204 143
NspI r_CATG'y
Cuts at: 0 721 1125
Size: 721 404
PflMI CCAn_nnn'nTGG
Cuts at: 0 114 1125
Size: 114 1011
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 16 48 1125
Size: 16 32 1077
Fragments arranged by size:
1077 32 16
PsiI TTA'TAA
Cuts at: 0 665 1125
Size: 665 460
PstI C_TGCA'G
Cuts at: 0 322 731 1125
Size: 322 409 394
Fragments arranged by size:
409 394 322
SacI G_AGCT'C
Cuts at: 0 638 1125
Size: 638 487
SapI GCTCTTCn'nnn_
Cuts at: 0 479 1125
Size: 479 646
SfcI C'TryA_G
Cuts at: 0 318 727 1125
Size: 318 409 398
Fragments arranged by size:
409 398 318
SmlI C'TyrA_G
Cuts at: 0 16 1125
Size: 16 1109
SspI AAT'ATT
Cuts at: 0 545 1125
Size: 545 580
SwaI ATTT'AAAT
Cuts at: 0 584 1125
Size: 584 541
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 883 951 1125
Size: 883 68 174
Fragments arranged by size:
883 174 68
TatI w'GTAC_w
Cuts at: 0 1074 1125
Size: 1074 51
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 146 178 1125
Size: 146 32 947
Fragments arranged by size:
947 146 32
XbaI T'CTAG_A
Cuts at: 0 255 1125
Size: 255 870
Enzymes that do cut:
AcuI AlfI AlwNI ApoI BanI BanII BbsI BglII
BpmI Bpu10I BpuEI BsaHI BsaXI BseRI BsgI BsiHKAI
Bsp1286I BsrGI BssSI BstAPI BstXI BstYI BtgI BtsI
CspCI DraI EarI EcoICRI Eco57MI FalI Hin4I HincII
HindIII MfeI MmeI MslI NspI PflMI PpiI PsiI
PstI SacI SapI SfcI SmlI SspI SwaI TaqII
TatI TstI XbaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AloI ApaI ApaLI AscI AseI
AsiSI AvaI AvrII BaeI BamHI BbeI BbvCI BcgI
BciVI BclI BfrBI BglI BlpI Bme1580I BmgBI BmrI
BmtI BplI BsaI BsaAI BsaBI BsaWI BseYI BsiEI
BsiWI BsmI BsmBI BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssHII BstBI BstEII BstZ17I Bsu36I BtgZI ClaI
DraIII DrdI EaeI EagI EciI EcoNI EcoO109I EcoRI
EcoRV FseI FspI FspAI HaeII HpaI KasI KpnI
MluI MscI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI PacI PasI PciI PfoI
PmeI PmlI PpuMI PshAI PspOMI PspXI PsrI PvuI
PvuII RsrII SacII SalI SanDI SbfI ScaI SexAI
SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI
SrfI StuI StyI Tth111I XcmI XhoI XmaI XmnI
ZraI