Restriction map for
pFN21AE3153
(Linear) (Six-base) MAPSORT of: KIEE3153.seq Check: 9131 from: 1 to: 1107
KIEE3153.orf
With 182 enzymes: SgfI *
December 27, 2011 16:33 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 874 1107
Size: 874 233
AleI CACnn'nnGTG
Cuts at: 0 766 1107
Size: 766 341
ApoI r'AATT_y
Cuts at: 0 81 185 452 1107
Size: 81 104 267 655
Fragments arranged by size:
655 267 104 81
BamHI G'GATC_C
Cuts at: 0 1052 1107
Size: 1052 55
BanII G_rGCy'C
Cuts at: 0 266 1107
Size: 266 841
BbsI GAAGACnn'nnnn_
Cuts at: 0 899 1107
Size: 899 208
BclI T'GATC_A
Cuts at: 0 269 1107
Size: 269 838
BfrBI ATG'CAT
Cuts at: 0 283 1107
Size: 283 824
BglII A'GATC_T
Cuts at: 0 875 1107
Size: 875 232
BlpI GC'TnA_GC
Cuts at: 0 599 1107
Size: 599 508
Bme1580I G_kGCm'C
Cuts at: 0 588 1000 1107
Size: 588 412 107
Fragments arranged by size:
588 412 107
BmgBI CAC'GTC
Cuts at: 0 243 1107
Size: 243 864
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 551 742 976 1107
Size: 551 191 234 131
Fragments arranged by size:
551 234 191 131
BsaI GGTCTCn'nnnn_
Cuts at: 0 841 1107
Size: 841 266
BsaBI GATnn'nnATC
Cuts at: 0 793 1107
Size: 793 314
BsaHI Gr'CG_yC
Cuts at: 0 13 914 1107
Size: 13 901 193
Fragments arranged by size:
901 193 13
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1057 1087 1107
Size: 1057 30 20
Fragments arranged by size:
1057 30 20
BseYI C'CCAG_C
Cuts at: 0 715 1107
Size: 715 392
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 166 1107
Size: 166 941
BsiHKAI G_wGCw'C
Cuts at: 0 266 1107
Size: 266 841
BsmI GAATG_Cn'
Cuts at: 0 549 675 1107
Size: 549 126 432
Fragments arranged by size:
549 432 126
Bsp1286I G_dGCh'C
Cuts at: 0 266 588 1000 1107
Size: 266 322 412 107
Fragments arranged by size:
412 322 266 107
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 138 1107
Size: 138 969
BsrBI CCG'CTC
Cuts at: 0 120 1107
Size: 120 987
BstAPI GCAn_nnn'nTGC
Cuts at: 0 347 1107
Size: 347 760
BstBI TT'CG_AA
Cuts at: 0 235 1107
Size: 235 872
BstEII G'GTnAC_C
Cuts at: 0 957 1107
Size: 957 150
BstXI CCAn_nnnn'nTGG
Cuts at: 0 258 801 1107
Size: 258 543 306
Fragments arranged by size:
543 306 258
BstYI r'GATC_y
Cuts at: 0 527 725 875 1052 1107
Size: 527 198 150 177 55
Fragments arranged by size:
527 198 177 150 55
BtgI C'CryG_G
Cuts at: 0 686 1107
Size: 686 421
BtsI GCAGTG_nn'
Cuts at: 0 352 629 1107
Size: 352 277 478
Fragments arranged by size:
478 352 277
EaeI y'GGCC_r
Cuts at: 0 557 1107
Size: 557 550
EarI CTCTTCn'nnn_
Cuts at: 0 518 574 926 1107
Size: 518 56 352 181
Fragments arranged by size:
518 352 181 56
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 939 1107
Size: 939 168
EcoICRI GAG'CTC
Cuts at: 0 264 1107
Size: 264 843
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 551 742 874 976 1107
Size: 551 191 132 102 131
Fragments arranged by size:
551 191 132 131 102
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1036 1107
Size: 1036 71
EcoRI G'AATT_C
Cuts at: 0 81 1107
Size: 81 1026
EcoRV GAT'ATC
Cuts at: 0 1020 1107
Size: 1020 87
HaeII r_GCGC'y
Cuts at: 0 606 1107
Size: 606 501
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 637 669 876 908 1057 1089 1107
Size: 637 32 207 32 149 32 18
Fragments arranged by size:
637 207 149 32 32 32 18
HincII GTy'rAC
Cuts at: 0 480 825 1107
Size: 480 345 282
Fragments arranged by size:
480 345 282
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 635 759 1030 1107
Size: 635 124 271 77
Fragments arranged by size:
635 271 124 77
MscI TGG'CCA
Cuts at: 0 559 1107
Size: 559 548
MslI CAynn'nnrTG
Cuts at: 0 256 316 766 799 1107
Size: 256 60 450 33 308
Fragments arranged by size:
450 308 256 60 33
NcoI C'CATG_G
Cuts at: 0 686 1107
Size: 686 421
NsiI A_TGCA'T
Cuts at: 0 285 1107
Size: 285 822
NspI r_CATG'y
Cuts at: 0 287 1107
Size: 287 820
PstI C_TGCA'G
Cuts at: 0 843 1107
Size: 843 264
SacI G_AGCT'C
Cuts at: 0 266 1107
Size: 266 841
SfcI C'TryA_G
Cuts at: 0 839 1107
Size: 839 268
SspI AAT'ATT
Cuts at: 0 471 1107
Size: 471 636
StyI C'CwwG_G
Cuts at: 0 686 1107
Size: 686 421
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 699 760 1107
Size: 699 61 347
Fragments arranged by size:
699 347 61
TatI w'GTAC_w
Cuts at: 0 180 1107
Size: 180 927
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 608 640 965 997 1107
Size: 608 32 325 32 110
Fragments arranged by size:
608 325 110 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 54 948 1107
Size: 54 894 159
Fragments arranged by size:
894 159 54
Enzymes that do cut:
AcuI AleI ApoI BamHI BanII BbsI BclI BfrBI
BglII BlpI Bme1580I BmgBI BpmI BsaI BsaBI BsaHI
BsaXI BseYI BsgI BsiHKAI BsmI Bsp1286I BspMI BsrBI
BstAPI BstBI BstEII BstXI BstYI BtgI BtsI EaeI
EarI EciI EcoICRI Eco57MI EcoNI EcoRI EcoRV HaeII
Hin4I HincII MmeI MscI MslI NcoI NsiI NspI
PstI SacI SfcI SspI StyI TaqII TatI TstI
XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AlfI AloI AlwNI ApaI ApaLI AscI
AseI AsiSI AvaI AvrII BaeI BanI BbeI BbvCI
BcgI BciVI BglI BmrI BmtI BplI Bpu10I BpuEI
BsaAI BsaWI BseRI BsiEI BsiWI BsmBI BspEI BspHI
BsrDI BsrFI BsrGI BssHII BssSI BstZ17I Bsu36I BtgZI
ClaI CspCI DraI DraIII DrdI EagI EcoO109I FalI
FseI FspI FspAI HindIII HpaI KasI KpnI MfeI
MluI MspA1I NaeI NarI NdeI NgoMIV NheI NotI
NruI PacI PasI PciI PflMI PfoI PmeI PmlI
PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PvuI
PvuII RsrII SacII SalI SanDI SapI SbfI ScaI
SexAI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SrfI StuI SwaI Tth111I XbaI XhoI
XmaI XmnI ZraI