Restriction map for
pFN21AE3149
(Linear) (Six-base) MAPSORT of: KIEE3149.seq Check: 3205 from: 1 to: 1095
KIEE3149.orf
With 182 enzymes: SgfI *
March 22, 2012 16:17 ..
Acc65I G'GTAC_C
Cuts at: 0 869 1095
Size: 869 226
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 660 1095
Size: 660 435
AleI CACnn'nnGTG
Cuts at: 0 222 1095
Size: 222 873
AlwNI CAG_nnn'CTG
Cuts at: 0 438 476 1095
Size: 438 38 619
Fragments arranged by size:
619 438 38
ApoI r'AATT_y
Cuts at: 0 211 1095
Size: 211 884
AvaI C'yCGr_G
Cuts at: 0 929 1095
Size: 929 166
BanI G'GyrC_C
Cuts at: 0 154 869 1095
Size: 154 715 226
Fragments arranged by size:
715 226 154
BbsI GAAGACnn'nnnn_
Cuts at: 0 1082 1095
Size: 1082 13
BclI T'GATC_A
Cuts at: 0 287 830 1095
Size: 287 543 265
Fragments arranged by size:
543 287 265
BfrBI ATG'CAT
Cuts at: 0 552 1095
Size: 552 543
BlpI GC'TnA_GC
Cuts at: 0 325 1095
Size: 325 770
BmrI ACTGGGnnnn_n'
Cuts at: 0 44 426 1095
Size: 44 382 669
Fragments arranged by size:
669 382 44
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 329 688 1095
Size: 329 359 407
Fragments arranged by size:
407 359 329
BsaI GGTCTCn'nnnn_
Cuts at: 0 909 1095
Size: 909 186
BsiHKAI G_wGCw'C
Cuts at: 0 567 1095
Size: 567 528
BsmI GAATG_Cn'
Cuts at: 0 554 794 1095
Size: 554 240 301
Fragments arranged by size:
554 301 240
Bsp1286I G_dGCh'C
Cuts at: 0 567 1095
Size: 567 528
BspHI T'CATG_A
Cuts at: 0 284 1095
Size: 284 811
BsrDI GCAATG_nn'
Cuts at: 0 120 239 833 1095
Size: 120 119 594 262
Fragments arranged by size:
594 262 120 119
BsrGI T'GTAC_A
Cuts at: 0 297 972 1095
Size: 297 675 123
Fragments arranged by size:
675 297 123
BstXI CCAn_nnnn'nTGG
Cuts at: 0 224 1095
Size: 224 871
ClaI AT'CG_AT
Cuts at: 0 824 1095
Size: 824 271
DraIII CAC_nnn'GTG
Cuts at: 0 434 1095
Size: 434 661
EaeI y'GGCC_r
Cuts at: 0 881 1003 1095
Size: 881 122 92
Fragments arranged by size:
881 122 92
EarI CTCTTCn'nnn_
Cuts at: 0 679 912 1095
Size: 679 233 183
Fragments arranged by size:
679 233 183
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 660 1095
Size: 660 435
EcoO109I rG'GnC_Cy
Cuts at: 0 309 1095
Size: 309 786
EcoRV GAT'ATC
Cuts at: 0 6 1095
Size: 6 1089
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 410 442 638 670 1095
Size: 410 32 196 32 425
Fragments arranged by size:
425 410 196 32 32
KpnI G_GTAC'C
Cuts at: 0 873 1095
Size: 873 222
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 396 1095
Size: 396 699
MscI TGG'CCA
Cuts at: 0 883 1095
Size: 883 212
MslI CAynn'nnrTG
Cuts at: 0 222 517 1095
Size: 222 295 578
Fragments arranged by size:
578 295 222
MspA1I CmG'CkG
Cuts at: 0 82 680 1008 1095
Size: 82 598 328 87
Fragments arranged by size:
598 328 87 82
NsiI A_TGCA'T
Cuts at: 0 554 1095
Size: 554 541
PflMI CCAn_nnn'nTGG
Cuts at: 0 879 1095
Size: 879 216
PpuMI rG'GwC_Cy
Cuts at: 0 309 1095
Size: 309 786
PshAI GACnn'nnGTC
Cuts at: 0 280 1095
Size: 280 815
SanDI GG'GwC_CC
Cuts at: 0 309 1095
Size: 309 786
SmlI C'TyrA_G
Cuts at: 0 929 1095
Size: 929 166
SspI AAT'ATT
Cuts at: 0 727 1095
Size: 727 368
StyI C'CwwG_G
Cuts at: 0 317 1095
Size: 317 778
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 445 1095
Size: 445 650
TatI w'GTAC_w
Cuts at: 0 297 972 1095
Size: 297 675 123
Fragments arranged by size:
675 297 123
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 224 1095
Size: 224 871
XhoI C'TCGA_G
Cuts at: 0 929 1095
Size: 929 166
Enzymes that do cut:
Acc65I AcuI AleI AlwNI ApoI AvaI BanI BbsI
BclI BfrBI BlpI BmrI BplI BsaI BsiHKAI BsmI
Bsp1286I BspHI BsrDI BsrGI BstXI ClaI DraIII EaeI
EarI Eco57MI EcoO109I EcoRV Hin4I KpnI MmeI MscI
MslI MspA1I NsiI PflMI PpuMI PshAI SanDI SmlI
SspI StyI TaqII TatI XcmI XhoI
Enzymes that do not cut:
AarI AatII AccI AclI AfeI AflII AflIII AgeI
AhdI AlfI AloI ApaI ApaLI AscI AseI AsiSI
AvrII BaeI BamHI BanII BbeI BbvCI BcgI BciVI
BglI BglII Bme1580I BmgBI BmtI BpmI Bpu10I BpuEI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiWI BsmBI BspEI BspMI BsrBI BsrFI BssHII
BssSI BstAPI BstBI BstEII BstYI BstZ17I Bsu36I BtgI
BtgZI BtsI CspCI DraI DrdI EagI EciI EcoICRI
EcoNI EcoRI FalI FseI FspI FspAI HaeII HincII
HindIII HpaI KasI MfeI MluI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI NspI PacI PasI
PciI PfoI PmeI PmlI PpiI PsiI PspOMI PspXI
PsrI PstI PvuI PvuII RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI
SgrAI SmaI SnaBI SpeI SphI SrfI StuI SwaI
TstI Tth111I XbaI XmaI XmnI ZraI