Restriction map for
pFN21AE3147
(Linear) (Six-base) MAPSORT of: KIEE3147.seq Check: 5124 from: 1 to: 1080
KIEE3147.orf
With 182 enzymes: SgfI *
December 27, 2011 16:33 ..
AatII G_ACGT'C
Cuts at: 0 404 1080
Size: 404 676
Acc65I G'GTAC_C
Cuts at: 0 670 1080
Size: 670 410
AhdI GACnn_n'nnGTC
Cuts at: 0 67 1080
Size: 67 1013
ApaI G_GGCC'C
Cuts at: 0 117 1080
Size: 117 963
ApaLI G'TGCA_C
Cuts at: 0 651 1080
Size: 651 429
ApoI r'AATT_y
Cuts at: 0 936 1080
Size: 936 144
AseI AT'TA_AT
Cuts at: 0 200 1080
Size: 200 880
AvrII C'CTAG_G
Cuts at: 0 420 1080
Size: 420 660
BanI G'GyrC_C
Cuts at: 0 670 1080
Size: 670 410
BanII G_rGCy'C
Cuts at: 0 27 46 117 1080
Size: 27 19 71 963
Fragments arranged by size:
963 71 27 19
BbsI GAAGACnn'nnnn_
Cuts at: 0 116 453 835 1080
Size: 116 337 382 245
Fragments arranged by size:
382 337 245 116
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 73 107 1080
Size: 73 34 973
Fragments arranged by size:
973 73 34
BclI T'GATC_A
Cuts at: 0 497 1080
Size: 497 583
BglI GCCn_nnn'nGGC
Cuts at: 0 304 1080
Size: 304 776
BglII A'GATC_T
Cuts at: 0 645 1080
Size: 645 435
BlpI GC'TnA_GC
Cuts at: 0 1016 1080
Size: 1016 64
Bme1580I G_kGCm'C
Cuts at: 0 117 655 1080
Size: 117 538 425
Fragments arranged by size:
538 425 117
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 732 1080
Size: 732 348
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 730 1080
Size: 730 350
Bpu10I CC'TnA_GC
Cuts at: 0 899 1080
Size: 899 181
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 789 1080
Size: 789 291
BsaAI yAC'GTr
Cuts at: 0 759 1080
Size: 759 321
BsaBI GATnn'nnATC
Cuts at: 0 545 1080
Size: 545 535
BsaHI Gr'CG_yC
Cuts at: 0 401 636 1080
Size: 401 235 444
Fragments arranged by size:
444 401 235
BsaWI w'CCGG_w
Cuts at: 0 557 629 1080
Size: 557 72 451
Fragments arranged by size:
557 451 72
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 794 1080
Size: 794 286
BseYI C'CCAG_C
Cuts at: 0 20 678 876 975 1080
Size: 20 658 198 99 105
Fragments arranged by size:
658 198 105 99 20
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1018 1080
Size: 1018 62
BsiEI CG_ry'CG
Cuts at: 0 31 1080
Size: 31 1049
BsiHKAI G_wGCw'C
Cuts at: 0 655 1080
Size: 655 425
Bsp1286I G_dGCh'C
Cuts at: 0 27 46 117 655 1080
Size: 27 19 71 538 425
Fragments arranged by size:
538 425 71 27 19
BspEI T'CCGG_A
Cuts at: 0 557 1080
Size: 557 523
BspHI T'CATG_A
Cuts at: 0 266 1080
Size: 266 814
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 806 1080
Size: 806 274
BsrGI T'GTAC_A
Cuts at: 0 254 1080
Size: 254 826
BstEII G'GTnAC_C
Cuts at: 0 180 1080
Size: 180 900
BstXI CCAn_nnnn'nTGG
Cuts at: 0 456 1080
Size: 456 624
BstYI r'GATC_y
Cuts at: 0 645 1080
Size: 645 435
BtgI C'CryG_G
Cuts at: 0 927 1080
Size: 927 153
DraIII CAC_nnn'GTG
Cuts at: 0 410 1041 1080
Size: 410 631 39
Fragments arranged by size:
631 410 39
EaeI y'GGCC_r
Cuts at: 0 885 1080
Size: 885 195
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 382 1080
Size: 382 698
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 730 1080
Size: 730 350
EcoO109I rG'GnC_Cy
Cuts at: 0 113 114 1080
Size: 113 1 966
Fragments arranged by size:
966 113 1
EcoRI G'AATT_C
Cuts at: 0 936 1080
Size: 936 144
EcoRV GAT'ATC
Cuts at: 0 543 693 1080
Size: 543 150 387
Fragments arranged by size:
543 387 150
HaeII r_GCGC'y
Cuts at: 0 7 1080
Size: 7 1073
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 53 85 1080
Size: 53 32 995
Fragments arranged by size:
995 53 32
HindIII A'AGCT_T
Cuts at: 0 130 1080
Size: 130 950
KpnI G_GTAC'C
Cuts at: 0 674 1080
Size: 674 406
MscI TGG'CCA
Cuts at: 0 887 1080
Size: 887 193
MslI CAynn'nnrTG
Cuts at: 0 950 1080
Size: 950 130
MspA1I CmG'CkG
Cuts at: 0 891 1080
Size: 891 189
PflMI CCAn_nnn'nTGG
Cuts at: 0 729 1080
Size: 729 351
PmlI CAC'GTG
Cuts at: 0 759 1080
Size: 759 321
PspOMI G'GGCC_C
Cuts at: 0 113 1080
Size: 113 967
PstI C_TGCA'G
Cuts at: 0 992 1003 1080
Size: 992 11 77
Fragments arranged by size:
992 77 11
PvuII CAG'CTG
Cuts at: 0 891 1080
Size: 891 189
SexAI A'CCwGG_T
Cuts at: 0 455 1080
Size: 455 625
SfcI C'TryA_G
Cuts at: 0 988 999 1080
Size: 988 11 81
Fragments arranged by size:
988 81 11
SmlI C'TyrA_G
Cuts at: 0 768 1080
Size: 768 312
StyI C'CwwG_G
Cuts at: 0 420 1065 1080
Size: 420 645 15
Fragments arranged by size:
645 420 15
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 17 110 587 692 1080
Size: 17 93 477 105 388
Fragments arranged by size:
477 388 105 93 17
TatI w'GTAC_w
Cuts at: 0 254 1080
Size: 254 826
ZraI GAC'GTC
Cuts at: 0 402 1080
Size: 402 678
Enzymes that do cut:
AatII Acc65I AhdI ApaI ApaLI ApoI AseI AvrII
BanI BanII BbsI BcgI BclI BglI BglII BlpI
Bme1580I BplI BpmI Bpu10I BpuEI BsaAI BsaBI BsaHI
BsaWI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrGI BstEII BstXI BstYI BtgI DraIII
EaeI EciI Eco57MI EcoO109I EcoRI EcoRV HaeII Hin4I
HindIII KpnI MscI MslI MspA1I PflMI PmlI PspOMI
PstI PvuII SexAI SfcI SmlI StyI TaqII TatI
ZraI
Enzymes that do not cut:
AarI AccI AclI AcuI AfeI AflII AflIII AgeI
AleI AlfI AloI AlwNI AscI AsiSI AvaI BaeI
BamHI BbeI BbvCI BciVI BfrBI BmgBI BmrI BmtI
BsaI BsaXI BsiWI BsmI BsmBI BsrBI BsrDI BsrFI
BssHII BssSI BstAPI BstBI BstZ17I Bsu36I BtgZI BtsI
ClaI CspCI DraI DrdI EagI EarI EcoICRI EcoNI
FalI FseI FspI FspAI HincII HpaI KasI MfeI
MluI MmeI NaeI NarI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspI PacI PasI PciI PfoI
PmeI PpiI PpuMI PshAI PsiI PspXI PsrI PvuI
RsrII SacI SacII SalI SanDI SapI SbfI ScaI
SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI
SrfI SspI StuI SwaI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI