Restriction map for
pFN21AE3146
(Linear) (Six-base) MAPSORT of: KIEE3146.seq Check: 5780 from: 1 to: 1071
KIEE3146.orf
With 182 enzymes: SgfI *
July 3, 2012 17:22 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 81 230 1071
Size: 81 149 841
Fragments arranged by size:
841 149 81
AlwNI CAG_nnn'CTG
Cuts at: 0 694 1071
Size: 694 377
ApoI r'AATT_y
Cuts at: 0 409 485 768 993 1071
Size: 409 76 283 225 78
Fragments arranged by size:
409 283 225 78 76
BanII G_rGCy'C
Cuts at: 0 816 1071
Size: 816 255
BbvCI CC'TCA_GC
Cuts at: 0 890 1071
Size: 890 181
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 394 436 521 1071
Size: 394 42 85 550
Fragments arranged by size:
550 394 85 42
Bpu10I CC'TnA_GC
Cuts at: 0 890 1071
Size: 890 181
BsaAI yAC'GTr
Cuts at: 0 864 1071
Size: 864 207
BsaWI w'CCGG_w
Cuts at: 0 271 1071
Size: 271 800
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 26 488 518 1071
Size: 26 462 30 553
Fragments arranged by size:
553 462 30 26
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 20 958 994 1071
Size: 20 938 36 77
Fragments arranged by size:
938 77 36 20
BseYI C'CCAG_C
Cuts at: 0 19 1071
Size: 19 1052
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 526 1071
Size: 526 545
Bsp1286I G_dGCh'C
Cuts at: 0 816 1071
Size: 816 255
BspHI T'CATG_A
Cuts at: 0 1013 1071
Size: 1013 58
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 435 1071
Size: 435 636
BsrDI GCAATG_nn'
Cuts at: 0 974 1071
Size: 974 97
DraI TTT'AAA
Cuts at: 0 53 1040 1071
Size: 53 987 31
Fragments arranged by size:
987 53 31
EarI CTCTTCn'nnn_
Cuts at: 0 902 920 935 1071
Size: 902 18 15 136
Fragments arranged by size:
902 136 18 15
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 81 230 394 436 521 1071
Size: 81 149 164 42 85 550
Fragments arranged by size:
550 164 149 85 81 42
EcoO109I rG'GnC_Cy
Cuts at: 0 307 1071
Size: 307 764
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 995 1027 1071
Size: 995 32 44
Fragments arranged by size:
995 44 32
HincII GTy'rAC
Cuts at: 0 584 1071
Size: 584 487
HindIII A'AGCT_T
Cuts at: 0 1043 1071
Size: 1043 28
HpaI GTT'AAC
Cuts at: 0 584 1071
Size: 584 487
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 528 1071
Size: 528 543
MslI CAynn'nnrTG
Cuts at: 0 344 476 1071
Size: 344 132 595
Fragments arranged by size:
595 344 132
PpuMI rG'GwC_Cy
Cuts at: 0 307 1071
Size: 307 764
SfcI C'TryA_G
Cuts at: 0 135 569 727 1071
Size: 135 434 158 344
Fragments arranged by size:
434 344 158 135
SnaBI TAC'GTA
Cuts at: 0 864 1071
Size: 864 207
SspI AAT'ATT
Cuts at: 0 536 1071
Size: 536 535
StyI C'CwwG_G
Cuts at: 0 362 794 1071
Size: 362 432 277
Fragments arranged by size:
432 362 277
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 834 1071
Size: 834 237
TatI w'GTAC_w
Cuts at: 0 283 1071
Size: 283 788
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 28 488 520 1071
Size: 28 460 32 551
Fragments arranged by size:
551 460 32 28
XbaI T'CTAG_A
Cuts at: 0 897 1071
Size: 897 174
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 369 1071
Size: 369 702
Enzymes that do cut:
AcuI AlwNI ApoI BanII BbvCI BpmI Bpu10I BsaAI
BsaWI BsaXI BseRI BseYI BsgI Bsp1286I BspHI BspMI
BsrDI DraI EarI Eco57MI EcoO109I Hin4I HincII HindIII
HpaI MmeI MslI PpuMI SfcI SnaBI SspI StyI
TaqII TatI TstI XbaI XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI ApaI ApaLI AscI
AseI AsiSI AvaI AvrII BaeI BamHI BanI BbeI
BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpuEI BsaI BsaBI
BsaHI BsiEI BsiHKAI BsiWI BsmI BsmBI BspEI BsrBI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraIII DrdI EaeI EagI EciI EcoICRI EcoNI EcoRI
EcoRV FalI FseI FspI FspAI HaeII KasI KpnI
MfeI MluI MscI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI NspI PacI PasI
PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI
PspOMI PspXI PsrI PstI PvuI PvuII RsrII SacI
SacII SalI SanDI SapI SbfI ScaI SexAI SfiI
SfoI SgfI SgrAI SmaI SmlI SpeI SphI SrfI
StuI SwaI Tth111I XhoI XmaI XmnI ZraI