Restriction map for
pFN21AE3144
(Linear) (Six-base) MAPSORT of: KIEE3144.seq Check: 489 from: 1 to: 1062
KIEE3144.orf
With 182 enzymes: SgfI *
March 22, 2012 16:16 ..
AccI GT'mk_AC
Cuts at: 0 300 1062
Size: 300 762
AhdI GACnn_n'nnGTC
Cuts at: 0 930 1062
Size: 930 132
AlwNI CAG_nnn'CTG
Cuts at: 0 217 645 1062
Size: 217 428 417
Fragments arranged by size:
428 417 217
ApaI G_GGCC'C
Cuts at: 0 55 172 1062
Size: 55 117 890
Fragments arranged by size:
890 117 55
AvaI C'yCGr_G
Cuts at: 0 55 171 196 488 1062
Size: 55 116 25 292 574
Fragments arranged by size:
574 292 116 55 25
BanI G'GyrC_C
Cuts at: 0 37 1062
Size: 37 1025
BanII G_rGCy'C
Cuts at: 0 55 172 203 604 677 1062
Size: 55 117 31 401 73 385
Fragments arranged by size:
401 385 117 73 55 31
BbeI G_GCGC'C
Cuts at: 0 41 1062
Size: 41 1021
BbvCI CC'TCA_GC
Cuts at: 0 210 1062
Size: 210 852
BciVI GTATCCnnnnn_n'
Cuts at: 0 739 1062
Size: 739 323
BclI T'GATC_A
Cuts at: 0 401 1062
Size: 401 661
BfrBI ATG'CAT
Cuts at: 0 397 1062
Size: 397 665
BglI GCCn_nnn'nGGC
Cuts at: 0 46 226 1062
Size: 46 180 836
Fragments arranged by size:
836 180 46
BlpI GC'TnA_GC
Cuts at: 0 238 259 1062
Size: 238 21 803
Fragments arranged by size:
803 238 21
Bme1580I G_kGCm'C
Cuts at: 0 55 172 1062
Size: 55 117 890
Fragments arranged by size:
890 117 55
BmrI ACTGGGnnnn_n'
Cuts at: 0 255 279 1062
Size: 255 24 783
Fragments arranged by size:
783 255 24
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 332 618 847 994 1062
Size: 332 286 229 147 68
Fragments arranged by size:
332 286 229 147 68
Bpu10I CC'TnA_GC
Cuts at: 0 210 1062
Size: 210 852
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 585 1062
Size: 585 477
BsaI GGTCTCn'nnnn_
Cuts at: 0 733 1062
Size: 733 329
BsaHI Gr'CG_yC
Cuts at: 0 38 1062
Size: 38 1024
BsaWI w'CCGG_w
Cuts at: 0 305 1062
Size: 305 757
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 34 335 609 1062
Size: 34 301 274 453
Fragments arranged by size:
453 301 274 34
BseYI C'CCAG_C
Cuts at: 0 603 1062
Size: 603 459
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 853 1062
Size: 853 209
BsiHKAI G_wGCw'C
Cuts at: 0 629 1062
Size: 629 433
BsmI GAATG_Cn'
Cuts at: 0 999 1062
Size: 999 63
Bsp1286I G_dGCh'C
Cuts at: 0 55 172 203 604 629 677 1062
Size: 55 117 31 401 25 48 385
Fragments arranged by size:
401 385 117 55 48 31 25
BspEI T'CCGG_A
Cuts at: 0 305 1062
Size: 305 757
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 600 1062
Size: 600 462
BsrBI CCG'CTC
Cuts at: 0 179 1062
Size: 179 883
BsrDI GCAATG_nn'
Cuts at: 0 555 1062
Size: 555 507
BssSI C'ACGA_G
Cuts at: 0 736 1062
Size: 736 326
Bsu36I CC'TnA_GG
Cuts at: 0 1054 1062
Size: 1054 8
BtgI C'CryG_G
Cuts at: 0 222 1062
Size: 222 840
BtsI GCAGTG_nn'
Cuts at: 0 301 1062
Size: 301 761
DrdI GACnn_nn'nnGTC
Cuts at: 0 959 1062
Size: 959 103
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 19 452 1062
Size: 19 433 610
Fragments arranged by size:
610 433 19
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 332 618 847 994 1062
Size: 332 286 229 147 68
Fragments arranged by size:
332 286 229 147 68
EcoNI CCTnn'n_nnAGG
Cuts at: 0 347 428 1062
Size: 347 81 634
Fragments arranged by size:
634 347 81
EcoO109I rG'GnC_Cy
Cuts at: 0 52 168 249 894 1062
Size: 52 116 81 645 168
Fragments arranged by size:
645 168 116 81 52
EcoRV GAT'ATC
Cuts at: 0 321 1062
Size: 321 741
HaeII r_GCGC'y
Cuts at: 0 41 1062
Size: 41 1021
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 310 342 740 772 916 948 1062
Size: 310 32 398 32 144 32 114
Fragments arranged by size:
398 310 144 114 32 32 32
HincII GTy'rAC
Cuts at: 0 378 1062
Size: 378 684
KasI G'GCGC_C
Cuts at: 0 37 1062
Size: 37 1025
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 37 290 1062
Size: 37 253 772
Fragments arranged by size:
772 253 37
MspA1I CmG'CkG
Cuts at: 0 98 154 192 217 1062
Size: 98 56 38 25 845
Fragments arranged by size:
845 98 56 38 25
NarI GG'CG_CC
Cuts at: 0 38 1062
Size: 38 1024
NsiI A_TGCA'T
Cuts at: 0 399 1062
Size: 399 663
PfoI T'CCnGG_A
Cuts at: 0 348 699 1062
Size: 348 351 363
Fragments arranged by size:
363 351 348
PpuMI rG'GwC_Cy
Cuts at: 0 249 894 1062
Size: 249 645 168
Fragments arranged by size:
645 249 168
PsiI TTA'TAA
Cuts at: 0 335 1062
Size: 335 727
PspOMI G'GGCC_C
Cuts at: 0 51 168 1062
Size: 51 117 894
Fragments arranged by size:
894 117 51
SanDI GG'GwC_CC
Cuts at: 0 249 1062
Size: 249 813
SfoI GGC'GCC
Cuts at: 0 39 1062
Size: 39 1023
SmaI CCC'GGG
Cuts at: 0 57 173 198 1062
Size: 57 116 25 864
Fragments arranged by size:
864 116 57 25
SmlI C'TyrA_G
Cuts at: 0 564 1062
Size: 564 498
SrfI GCCC'GGGC
Cuts at: 0 198 1062
Size: 198 864
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 497 1062
Size: 497 565
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 763 795 1062
Size: 763 32 267
Fragments arranged by size:
763 267 32
XmaI C'CCGG_G
Cuts at: 0 55 171 196 1062
Size: 55 116 25 866
Fragments arranged by size:
866 116 55 25
Enzymes that do cut:
AccI AhdI AlwNI ApaI AvaI BanI BanII BbeI
BbvCI BciVI BclI BfrBI BglI BlpI Bme1580I BmrI
BpmI Bpu10I BpuEI BsaI BsaHI BsaWI BseRI BseYI
BsgI BsiHKAI BsmI Bsp1286I BspEI BspMI BsrBI BsrDI
BssSI Bsu36I BtgI BtsI DrdI EciI Eco57MI EcoNI
EcoO109I EcoRV HaeII Hin4I HincII KasI MmeI MspA1I
NarI NsiI PfoI PpuMI PsiI PspOMI SanDI SfoI
SmaI SmlI SrfI TaqII TstI XmaI
Enzymes that do not cut:
AarI AatII Acc65I AclI AcuI AfeI AflII AflIII
AgeI AleI AlfI AloI ApaLI ApoI AscI AseI
AsiSI AvrII BaeI BamHI BbsI BcgI BglII BmgBI
BmtI BplI BsaAI BsaBI BsaXI BsiEI BsiWI BsmBI
BspHI BsrFI BsrGI BssHII BstAPI BstBI BstEII BstXI
BstYI BstZ17I BtgZI ClaI CspCI DraI DraIII EaeI
EagI EarI EcoICRI EcoRI FalI FseI FspI FspAI
HindIII HpaI KpnI MfeI MluI MscI MslI NaeI
NcoI NdeI NgoMIV NheI NotI NruI NspI PacI
PasI PciI PflMI PmeI PmlI PpiI PshAI PspXI
PsrI PstI PvuI PvuII RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfcI SfiI SgfI SgrAI
SnaBI SpeI SphI SspI StuI StyI SwaI TatI
Tth111I XbaI XcmI XhoI XmnI ZraI