Restriction map for
pFN21AE3136
(Linear) (Six-base) MAPSORT of: KIEE3136.seq Check: 4906 from: 1 to: 1044
KIEE3136.orf
With 182 enzymes: SgfI *
July 3, 2012 17:22 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 399 1044
Size: 399 645
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 796 1044
Size: 796 248
AlwNI CAG_nnn'CTG
Cuts at: 0 186 1044
Size: 186 858
AvaI C'yCGr_G
Cuts at: 0 200 283 1044
Size: 200 83 761
Fragments arranged by size:
761 200 83
BanI G'GyrC_C
Cuts at: 0 42 103 166 1044
Size: 42 61 63 878
Fragments arranged by size:
878 63 61 42
BbeI G_GCGC'C
Cuts at: 0 46 1044
Size: 46 998
BbsI GAAGACnn'nnnn_
Cuts at: 0 329 371 1044
Size: 329 42 673
Fragments arranged by size:
673 329 42
BciVI GTATCCnnnnn_n'
Cuts at: 0 268 303 711 1044
Size: 268 35 408 333
Fragments arranged by size:
408 333 268 35
BclI T'GATC_A
Cuts at: 0 353 1044
Size: 353 691
BfrBI ATG'CAT
Cuts at: 0 959 1044
Size: 959 85
Bme1580I G_kGCm'C
Cuts at: 0 537 1044
Size: 537 507
BmrI ACTGGGnnnn_n'
Cuts at: 0 613 1044
Size: 613 431
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 199 1044
Size: 199 845
BsaHI Gr'CG_yC
Cuts at: 0 43 62 549 1044
Size: 43 19 487 495
Fragments arranged by size:
495 487 43 19
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 527 674 833 849 1044
Size: 527 147 159 16 195
Fragments arranged by size:
527 195 159 147 16
BseYI C'CCAG_C
Cuts at: 0 462 1044
Size: 462 582
BsmBI CGTCTCn'nnnn_
Cuts at: 0 69 540 1044
Size: 69 471 504
Fragments arranged by size:
504 471 69
Bsp1286I G_dGCh'C
Cuts at: 0 537 1044
Size: 537 507
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 399 1044
Size: 399 645
BsrDI GCAATG_nn'
Cuts at: 0 309 1044
Size: 309 735
BsrGI T'GTAC_A
Cuts at: 0 882 1044
Size: 882 162
BstXI CCAn_nnnn'nTGG
Cuts at: 0 456 1044
Size: 456 588
BstYI r'GATC_y
Cuts at: 0 294 1044
Size: 294 750
BtgI C'CryG_G
Cuts at: 0 98 455 1044
Size: 98 357 589
Fragments arranged by size:
589 357 98
ClaI AT'CG_AT
Cuts at: 0 676 1044
Size: 676 368
DraIII CAC_nnn'GTG
Cuts at: 0 137 1044
Size: 137 907
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 199 796 1044
Size: 199 597 248
Fragments arranged by size:
597 248 199
EcoRV GAT'ATC
Cuts at: 0 674 1044
Size: 674 370
HaeII r_GCGC'y
Cuts at: 0 46 1044
Size: 46 998
HincII GTy'rAC
Cuts at: 0 939 1044
Size: 939 105
KasI G'GCGC_C
Cuts at: 0 42 1044
Size: 42 1002
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 252 1044
Size: 252 792
MspA1I CmG'CkG
Cuts at: 0 100 149 1044
Size: 100 49 895
Fragments arranged by size:
895 100 49
NarI GG'CG_CC
Cuts at: 0 43 1044
Size: 43 1001
NsiI A_TGCA'T
Cuts at: 0 961 1044
Size: 961 83
NspI r_CATG'y
Cuts at: 0 351 1009 1044
Size: 351 658 35
Fragments arranged by size:
658 351 35
RsrII CG'GwC_CG
Cuts at: 0 125 1044
Size: 125 919
SacII CC_GC'GG
Cuts at: 0 101 1044
Size: 101 943
ScaI AGT'ACT
Cuts at: 0 625 1044
Size: 625 419
SfcI C'TryA_G
Cuts at: 0 588 1044
Size: 588 456
SfoI GGC'GCC
Cuts at: 0 44 1044
Size: 44 1000
SmaI CCC'GGG
Cuts at: 0 202 1044
Size: 202 842
SpeI A'CTAG_T
Cuts at: 0 487 1044
Size: 487 557
StyI C'CwwG_G
Cuts at: 0 158 1010 1044
Size: 158 852 34
Fragments arranged by size:
852 158 34
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 142 184 1044
Size: 142 42 860
Fragments arranged by size:
860 142 42
TatI w'GTAC_w
Cuts at: 0 494 623 882 1044
Size: 494 129 259 162
Fragments arranged by size:
494 259 162 129
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 43 1030 1044
Size: 43 987 14
Fragments arranged by size:
987 43 14
XmaI C'CCGG_G
Cuts at: 0 200 1044
Size: 200 844
Enzymes that do cut:
AarI AcuI AlwNI AvaI BanI BbeI BbsI BciVI
BclI BfrBI Bme1580I BmrI BpmI BsaHI BseRI BseYI
BsmBI Bsp1286I BspMI BsrDI BsrGI BstXI BstYI BtgI
ClaI DraIII Eco57MI EcoRV HaeII HincII KasI MmeI
MspA1I NarI NsiI NspI RsrII SacII ScaI SfcI
SfoI SmaI SpeI StyI TaqII TatI XcmI XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI ApaI ApaLI ApoI AscI
AseI AsiSI AvrII BaeI BamHI BanII BbvCI BcgI
BglI BglII BlpI BmgBI BmtI BplI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaWI BsaXI BsgI BsiEI BsiHKAI
BsiWI BsmI BspEI BspHI BsrBI BsrFI BssHII BssSI
BstAPI BstBI BstEII BstZ17I Bsu36I BtgZI BtsI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI EcoNI
EcoO109I EcoRI FalI FseI FspI FspAI Hin4I HindIII
HpaI KpnI MfeI MluI MscI MslI NaeI NcoI
NdeI NgoMIV NheI NotI NruI PacI PasI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI
PspOMI PspXI PsrI PstI PvuI PvuII SacI SalI
SanDI SapI SbfI SexAI SfiI SgfI SgrAI SmlI
SnaBI SphI SrfI SspI StuI SwaI TstI Tth111I
XbaI XhoI XmnI ZraI