Restriction map for
pFN21AE3135
(Linear) (Six-base) MAPSORT of: KIEE3135.seq Check: 4024 from: 1 to: 1035
KIEE3135.orf
With 182 enzymes: SgfI *
December 27, 2011 16:33 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 232 1035
Size: 232 803
AlwNI CAG_nnn'CTG
Cuts at: 0 892 1035
Size: 892 143
ApoI r'AATT_y
Cuts at: 0 199 264 378 427 728 1035
Size: 199 65 114 49 301 307
Fragments arranged by size:
307 301 199 114 65 49
AvaI C'yCGr_G
Cuts at: 0 187 1035
Size: 187 848
BanII G_rGCy'C
Cuts at: 0 20 1035
Size: 20 1015
BglI GCCn_nnn'nGGC
Cuts at: 0 15 1035
Size: 15 1020
BglII A'GATC_T
Cuts at: 0 905 1035
Size: 905 130
BmrI ACTGGGnnnn_n'
Cuts at: 0 180 1035
Size: 180 855
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 532 1035
Size: 532 503
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 232 1035
Size: 232 803
BseYI C'CCAG_C
Cuts at: 0 57 1035
Size: 57 978
BsiEI CG_ry'CG
Cuts at: 0 10 144 1035
Size: 10 134 891
Fragments arranged by size:
891 134 10
BsiHKAI G_wGCw'C
Cuts at: 0 510 1035
Size: 510 525
Bsp1286I G_dGCh'C
Cuts at: 0 20 510 1035
Size: 20 490 525
Fragments arranged by size:
525 490 20
BspHI T'CATG_A
Cuts at: 0 933 1035
Size: 933 102
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 900 1035
Size: 900 135
BsrDI GCAATG_nn'
Cuts at: 0 305 356 566 1035
Size: 305 51 210 469
Fragments arranged by size:
469 305 210 51
BsrFI r'CCGG_y
Cuts at: 0 9 1035
Size: 9 1026
BsrGI T'GTAC_A
Cuts at: 0 854 866 1035
Size: 854 12 169
Fragments arranged by size:
854 169 12
BstAPI GCAn_nnn'nTGC
Cuts at: 0 367 1035
Size: 367 668
BstYI r'GATC_y
Cuts at: 0 282 905 1035
Size: 282 623 130
Fragments arranged by size:
623 282 130
BtgI C'CryG_G
Cuts at: 0 105 1035
Size: 105 930
BtsI GCAGTG_nn'
Cuts at: 0 656 857 1035
Size: 656 201 178
Fragments arranged by size:
656 201 178
DraI TTT'AAA
Cuts at: 0 533 1035
Size: 533 502
EaeI y'GGCC_r
Cuts at: 0 7 72 1035
Size: 7 65 963
Fragments arranged by size:
963 65 7
EagI C'GGCC_G
Cuts at: 0 7 1035
Size: 7 1028
EarI CTCTTCn'nnn_
Cuts at: 0 291 1035
Size: 291 744
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 232 1035
Size: 232 803
EcoNI CCTnn'n_nnAGG
Cuts at: 0 214 1035
Size: 214 821
EcoO109I rG'GnC_Cy
Cuts at: 0 54 1035
Size: 54 981
EcoRI G'AATT_C
Cuts at: 0 427 1035
Size: 427 608
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 448 1035
Size: 448 587
HaeII r_GCGC'y
Cuts at: 0 27 1035
Size: 27 1008
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 536 568 1035
Size: 536 32 467
Fragments arranged by size:
536 467 32
HincII GTy'rAC
Cuts at: 0 738 1035
Size: 738 297
HpaI GTT'AAC
Cuts at: 0 738 1035
Size: 738 297
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 204 211 1035
Size: 204 7 824
Fragments arranged by size:
824 204 7
MspA1I CmG'CkG
Cuts at: 0 61 107 844 955 1035
Size: 61 46 737 111 80
Fragments arranged by size:
737 111 80 61 46
NaeI GCC'GGC
Cuts at: 0 11 1035
Size: 11 1024
NgoMIV G'CCGG_C
Cuts at: 0 9 1035
Size: 9 1026
PfoI T'CCnGG_A
Cuts at: 0 744 1035
Size: 744 291
PpuMI rG'GwC_Cy
Cuts at: 0 54 1035
Size: 54 981
PvuII CAG'CTG
Cuts at: 0 844 955 1035
Size: 844 111 80
Fragments arranged by size:
844 111 80
SacII CC_GC'GG
Cuts at: 0 108 1035
Size: 108 927
SanDI GG'GwC_CC
Cuts at: 0 54 1035
Size: 54 981
SexAI A'CCwGG_T
Cuts at: 0 458 1035
Size: 458 577
SmlI C'TyrA_G
Cuts at: 0 511 1035
Size: 511 524
SspI AAT'ATT
Cuts at: 0 135 316 1035
Size: 135 181 719
Fragments arranged by size:
719 181 135
StyI C'CwwG_G
Cuts at: 0 40 1035
Size: 40 995
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 477 772 1035
Size: 477 295 263
Fragments arranged by size:
477 295 263
TatI w'GTAC_w
Cuts at: 0 854 866 1035
Size: 854 12 169
Fragments arranged by size:
854 169 12
XmnI GAAnn'nnTTC
Cuts at: 0 136 431 1035
Size: 136 295 604
Fragments arranged by size:
604 295 136
Enzymes that do cut:
AcuI AlwNI ApoI AvaI BanII BglI BglII BmrI
BpuEI BseRI BseYI BsiEI BsiHKAI Bsp1286I BspHI BspMI
BsrDI BsrFI BsrGI BstAPI BstYI BtgI BtsI DraI
EaeI EagI EarI Eco57MI EcoNI EcoO109I EcoRI FalI
HaeII Hin4I HincII HpaI MmeI MspA1I NaeI NgoMIV
PfoI PpuMI PvuII SacII SanDI SexAI SmlI SspI
StyI TaqII TatI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI ApaI ApaLI AscI
AseI AsiSI AvrII BaeI BamHI BanI BbeI BbsI
BbvCI BcgI BciVI BclI BfrBI BlpI Bme1580I BmgBI
BmtI BplI BpmI Bpu10I BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BsgI BsiWI BsmI BsmBI BspEI BsrBI
BssHII BssSI BstBI BstEII BstXI BstZ17I Bsu36I BtgZI
ClaI CspCI DraIII DrdI EciI EcoICRI EcoRV FseI
FspI FspAI HindIII KasI KpnI MfeI MluI MscI
MslI NarI NcoI NdeI NheI NotI NruI NsiI
NspI PacI PasI PciI PflMI PmeI PmlI PpiI
PshAI PsiI PspOMI PspXI PsrI PstI PvuI RsrII
SacI SalI SapI SbfI ScaI SfcI SfiI SfoI
SgfI SgrAI SmaI SnaBI SpeI SphI SrfI StuI
SwaI TstI Tth111I XbaI XcmI XhoI XmaI ZraI