(Linear) (Six-base) MAPSORT of: KIEE3132.seq  Check: 4831  from: 1  to: 1008



KIEE3132.orf

 With 182 enzymes: SgfI * 

                        December 27, 2011 16:33  ..

AccI GT'mk_AC

Cuts at:      0     367    1008 
   Size:        367     641
 
Acc65I G'GTAC_C

Cuts at:      0     394    1008 
   Size:        394     614
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     115     256    1008 
   Size:        115     141     752

  Fragments arranged by size: 

                 752     141     115
 
AflII C'TTAA_G

Cuts at:      0     145     166    1008 
   Size:        145      21     842

  Fragments arranged by size: 

                 842     145      21
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     131    1008 
   Size:        131     877
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      17      49    1008 
   Size:         17      32     959

  Fragments arranged by size: 

                 959      32      17
 
ApoI r'AATT_y

Cuts at:      0     281    1008 
   Size:        281     727
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     368     401    1008 
   Size:        368      33     607

  Fragments arranged by size: 

                 607     368      33
 
BanI G'GyrC_C

Cuts at:      0     394    1008 
   Size:        394     614
 
BanII G_rGCy'C

Cuts at:      0     981    1008 
   Size:        981      27
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     533     567    1008 
   Size:        533      34     441

  Fragments arranged by size: 

                 533     441      34
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     124     833    1008 
   Size:        124     709     175

  Fragments arranged by size: 

                 709     175     124
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     725    1008 
   Size:        725     283
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     782    1008 
   Size:        782     226
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     137     313    1008 
   Size:        137     176     695

  Fragments arranged by size: 

                 695     176     137
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0      64     419    1008 
   Size:         64     355     589

  Fragments arranged by size: 

                 589     355      64
 
Bsp1286I G_dGCh'C

Cuts at:      0     981    1008 
   Size:        981      27
 
BspHI T'CATG_A

Cuts at:      0      44    1008 
   Size:         44     964
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     723    1008 
   Size:        723     285
 
BsrDI GCAATG_nn'

Cuts at:      0     752    1008 
   Size:        752     256
 
BstBI TT'CG_AA

Cuts at:      0     543    1008 
   Size:        543     465
 
BstYI r'GATC_y

Cuts at:      0     537     890    1008 
   Size:        537     353     118

  Fragments arranged by size: 

                 537     353     118
 
BtgI C'CryG_G

Cuts at:      0      29    1008 
   Size:         29     979
 
BtsI GCAGTG_nn'

Cuts at:      0     325    1008 
   Size:        325     683
 
EarI CTCTTCn'nnn_

Cuts at:      0      91     197    1008 
   Size:         91     106     811

  Fragments arranged by size: 

                 811     106      91
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     115     124     256     833    1008 
   Size:        115       9     132     577     175

  Fragments arranged by size: 

                 577     175     132     115       9
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     666     698    1008 
   Size:        666      32     310

  Fragments arranged by size: 

                 666     310      32
 
KpnI G_GTAC'C

Cuts at:      0     398    1008 
   Size:        398     610
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     594    1008 
   Size:        594     414
 
MslI CAynn'nnrTG

Cuts at:      0     578     911    1008 
   Size:        578     333      97

  Fragments arranged by size: 

                 578     333      97
 
PfoI T'CCnGG_A

Cuts at:      0     810    1008 
   Size:        810     198
 
PshAI GACnn'nnGTC

Cuts at:      0     637    1008 
   Size:        637     371
 
SfcI C'TryA_G

Cuts at:      0     368    1008 
   Size:        368     640
 
SmlI C'TyrA_G

Cuts at:      0     145     166     740    1008 
   Size:        145      21     574     268

  Fragments arranged by size: 

                 574     268     145      21
 
StyI C'CwwG_G

Cuts at:      0     628    1008 
   Size:        628     380
 

 Enzymes that do cut:

     AccI   Acc65I     AcuI    AflII     AlfI     AloI     ApoI     BaeI
     BanI    BanII     BcgI     BpmI    BpuEI    BseRI     BsgI    BsmBI
 Bsp1286I    BspHI    BspMI    BsrDI    BstBI    BstYI     BtgI     BtsI
     EarI  Eco57MI    Hin4I     KpnI     MmeI     MslI     PfoI    PshAI
     SfcI     SmlI     StyI

 Enzymes that do not cut: 

     AarI    AatII     AclI     AfeI   AflIII     AgeI     AhdI     AleI
    AlwNI     ApaI    ApaLI     AscI     AseI    AsiSI     AvaI    AvrII
    BamHI     BbeI     BbsI    BbvCI    BciVI     BclI    BfrBI     BglI
    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI   Bpu10I
     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseYI    BsiEI
  BsiHKAI    BsiWI     BsmI    BspEI    BsrBI    BsrFI    BsrGI   BssHII
    BssSI   BstAPI   BstEII    BstXI  BstZ17I   Bsu36I    BtgZI     ClaI
    CspCI     DraI   DraIII     DrdI     EaeI     EagI     EciI  EcoICRI
    EcoNI EcoO109I    EcoRI    EcoRV     FalI     FseI     FspI    FspAI
    HaeII   HincII  HindIII     HpaI     KasI     MfeI     MluI     MscI
   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI
     NruI     NsiI     NspI     PacI     PasI     PciI    PflMI     PmeI
     PmlI     PpiI    PpuMI     PsiI   PspOMI    PspXI     PsrI     PstI
     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI     SapI
     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI
    SnaBI     SpeI     SphI     SrfI     SspI     StuI     SwaI    TaqII
     TatI     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI
     ZraI