Restriction map for
pFN21AE3131
(Linear) (Six-base) MAPSORT of: KIEE3131.seq Check: 7450 from: 1 to: 987
KIEE3131.orf
With 182 enzymes: SgfI *
March 22, 2012 16:16 ..
AatII G_ACGT'C
Cuts at: 0 617 987
Size: 617 370
AfeI AGC'GCT
Cuts at: 0 161 541 987
Size: 161 380 446
Fragments arranged by size:
446 380 161
AflIII A'CryG_T
Cuts at: 0 933 987
Size: 933 54
ApaI G_GGCC'C
Cuts at: 0 255 909 987
Size: 255 654 78
Fragments arranged by size:
654 255 78
AvaI C'yCGr_G
Cuts at: 0 473 987
Size: 473 514
BamHI G'GATC_C
Cuts at: 0 332 987
Size: 332 655
BanI G'GyrC_C
Cuts at: 0 549 987
Size: 549 438
BanII G_rGCy'C
Cuts at: 0 99 255 474 909 987
Size: 99 156 219 435 78
Fragments arranged by size:
435 219 156 99 78
BbsI GAAGACnn'nnnn_
Cuts at: 0 72 339 714 987
Size: 72 267 375 273
Fragments arranged by size:
375 273 267 72
BbvCI CC'TCA_GC
Cuts at: 0 884 987
Size: 884 103
BglII A'GATC_T
Cuts at: 0 168 746 987
Size: 168 578 241
Fragments arranged by size:
578 241 168
Bme1580I G_kGCm'C
Cuts at: 0 255 554 909 987
Size: 255 299 355 78
Fragments arranged by size:
355 299 255 78
BmrI ACTGGGnnnn_n'
Cuts at: 0 594 911 987
Size: 594 317 76
Fragments arranged by size:
594 317 76
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 388 987
Size: 388 599
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 337 409 649 655 727 828 987
Size: 337 72 240 6 72 101 159
Fragments arranged by size:
337 240 159 101 72 72 6
Bpu10I CC'TnA_GC
Cuts at: 0 446 884 965 987
Size: 446 438 81 22
Fragments arranged by size:
446 438 81 22
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 262 987
Size: 262 725
BsaI GGTCTCn'nnnn_
Cuts at: 0 307 359 987
Size: 307 52 628
Fragments arranged by size:
628 307 52
BsaHI Gr'CG_yC
Cuts at: 0 215 614 987
Size: 215 399 373
Fragments arranged by size:
399 373 215
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 730 987
Size: 730 257
BseYI C'CCAG_C
Cuts at: 0 90 228 529 925 987
Size: 90 138 301 396 62
Fragments arranged by size:
396 301 138 90 62
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 498 987
Size: 498 489
Bsp1286I G_dGCh'C
Cuts at: 0 99 255 474 554 909 987
Size: 99 156 219 80 355 78
Fragments arranged by size:
355 219 156 99 80 78
BssSI C'ACGA_G
Cuts at: 0 775 987
Size: 775 212
BstXI CCAn_nnnn'nTGG
Cuts at: 0 751 987
Size: 751 236
BstYI r'GATC_y
Cuts at: 0 168 332 746 987
Size: 168 164 414 241
Fragments arranged by size:
414 241 168 164
Bsu36I CC'TnA_GG
Cuts at: 0 32 987
Size: 32 955
BtgI C'CryG_G
Cuts at: 0 872 987
Size: 872 115
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 717 987
Size: 717 270
BtsI GCAGTG_nn'
Cuts at: 0 800 987
Size: 800 187
DraI TTT'AAA
Cuts at: 0 978 987
Size: 978 9
DraIII CAC_nnn'GTG
Cuts at: 0 602 987
Size: 602 385
EarI CTCTTCn'nnn_
Cuts at: 0 385 987
Size: 385 602
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 337 409 649 655 727 828 987
Size: 337 72 240 6 72 101 159
Fragments arranged by size:
337 240 159 101 72 72 6
EcoO109I rG'GnC_Cy
Cuts at: 0 251 252 478 573 864 880 905 906
Size: 251 1 226 95 291 16 25 1
Cuts at: 906 987
Size: 81
Fragments arranged by size:
291 251 226 95 81 25 16 1
1
HaeII r_GCGC'y
Cuts at: 0 163 543 987
Size: 163 380 444
Fragments arranged by size:
444 380 163
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 853 885 987
Size: 853 32 102
Fragments arranged by size:
853 102 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 189 987
Size: 189 798
MslI CAynn'nnrTG
Cuts at: 0 223 987
Size: 223 764
NspI r_CATG'y
Cuts at: 0 937 987
Size: 937 50
PciI A'CATG_T
Cuts at: 0 933 987
Size: 933 54
PflMI CCAn_nnn'nTGG
Cuts at: 0 488 987
Size: 488 499
PfoI T'CCnGG_A
Cuts at: 0 257 314 987
Size: 257 57 673
Fragments arranged by size:
673 257 57
PpuMI rG'GwC_Cy
Cuts at: 0 573 864 987
Size: 573 291 123
Fragments arranged by size:
573 291 123
PspOMI G'GGCC_C
Cuts at: 0 251 905 987
Size: 251 654 82
Fragments arranged by size:
654 251 82
PstI C_TGCA'G
Cuts at: 0 695 987
Size: 695 292
SanDI GG'GwC_CC
Cuts at: 0 573 987
Size: 573 414
SfcI C'TryA_G
Cuts at: 0 691 987
Size: 691 296
SmlI C'TyrA_G
Cuts at: 0 277 987
Size: 277 710
StuI AGG'CCT
Cuts at: 0 179 403 987
Size: 179 224 584
Fragments arranged by size:
584 224 179
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 75 289 613 939 940 987
Size: 75 214 324 326 1 47
Fragments arranged by size:
326 324 214 75 47 1
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 666 987
Size: 666 321
XmnI GAAnn'nnTTC
Cuts at: 0 55 268 987
Size: 55 213 719
Fragments arranged by size:
719 213 55
ZraI GAC'GTC
Cuts at: 0 615 987
Size: 615 372
Enzymes that do cut:
AatII AfeI AflIII ApaI AvaI BamHI BanI BanII
BbsI BbvCI BglII Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaHI BseRI BseYI BsgI Bsp1286I BssSI
BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI DraIII
EarI Eco57MI EcoO109I HaeII Hin4I MmeI MslI NspI
PciI PflMI PfoI PpuMI PspOMI PstI SanDI SfcI
SmlI StuI TaqII XcmI XmnI ZraI
Enzymes that do not cut:
AarI AccI Acc65I AclI AcuI AflII AgeI AhdI
AleI AlfI AloI AlwNI ApaLI ApoI AscI AseI
AsiSI AvrII BaeI BbeI BcgI BciVI BclI BfrBI
BglI BlpI BmgBI BmtI BsaAI BsaBI BsaWI BsaXI
BsiEI BsiHKAI BsiWI BsmI BsmBI BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BstAPI BstBI BstEII
BstZ17I ClaI CspCI DrdI EaeI EagI EciI EcoICRI
EcoNI EcoRI EcoRV FalI FseI FspI FspAI HincII
HindIII HpaI KasI KpnI MfeI MluI MscI MspA1I
NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI
NsiI PacI PasI PmeI PmlI PpiI PshAI PsiI
PspXI PsrI PvuI PvuII RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI
SmaI SnaBI SpeI SphI SrfI SspI StyI SwaI
TatI TstI Tth111I XbaI XhoI XmaI