Restriction map for
pFN21AE3125
(Linear) (Six-base) MAPSORT of: KIEE3125.seq Check: 4357 from: 1 to: 948
KIEE3125.orf
With 182 enzymes: SgfI *
December 27, 2011 16:33 ..
AccI GT'mk_AC
Cuts at: 0 429 948
Size: 429 519
AclI AA'CG_TT
Cuts at: 0 318 948
Size: 318 630
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 119 896 908 948
Size: 119 777 12 40
Fragments arranged by size:
777 119 40 12
AflIII A'CryG_T
Cuts at: 0 407 948
Size: 407 541
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 140 948
Size: 140 808
AlwNI CAG_nnn'CTG
Cuts at: 0 114 948
Size: 114 834
AvaI C'yCGr_G
Cuts at: 0 740 948
Size: 740 208
BanII G_rGCy'C
Cuts at: 0 333 948
Size: 333 615
BbsI GAAGACnn'nnnn_
Cuts at: 0 596 948
Size: 596 352
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 164 198 948
Size: 164 34 750
Fragments arranged by size:
750 164 34
BmrI ACTGGGnnnn_n'
Cuts at: 0 222 948
Size: 222 726
BsaBI GATnn'nnATC
Cuts at: 0 665 948
Size: 665 283
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 88 948
Size: 88 860
BseYI C'CCAG_C
Cuts at: 0 305 948
Size: 305 643
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 302 948
Size: 302 646
BsiHKAI G_wGCw'C
Cuts at: 0 184 333 948
Size: 184 149 615
Fragments arranged by size:
615 184 149
BsmI GAATG_Cn'
Cuts at: 0 710 948
Size: 710 238
Bsp1286I G_dGCh'C
Cuts at: 0 184 333 948
Size: 184 149 615
Fragments arranged by size:
615 184 149
BstBI TT'CG_AA
Cuts at: 0 447 948
Size: 447 501
BstYI r'GATC_y
Cuts at: 0 152 491 948
Size: 152 339 457
Fragments arranged by size:
457 339 152
ClaI AT'CG_AT
Cuts at: 0 122 386 948
Size: 122 264 562
Fragments arranged by size:
562 264 122
EaeI y'GGCC_r
Cuts at: 0 158 830 948
Size: 158 672 118
Fragments arranged by size:
672 158 118
EarI CTCTTCn'nnn_
Cuts at: 0 232 527 948
Size: 232 295 421
Fragments arranged by size:
421 295 232
EcoICRI GAG'CTC
Cuts at: 0 331 948
Size: 331 617
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 119 896 908 948
Size: 119 777 12 40
Fragments arranged by size:
777 119 40 12
EcoRV GAT'ATC
Cuts at: 0 852 948
Size: 852 96
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 109 364 948
Size: 109 255 584
Fragments arranged by size:
584 255 109
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 368 400 841 873 948
Size: 368 32 441 32 75
Fragments arranged by size:
441 368 75 32 32
MscI TGG'CCA
Cuts at: 0 160 832 948
Size: 160 672 116
Fragments arranged by size:
672 160 116
NspI r_CATG'y
Cuts at: 0 411 948
Size: 411 537
PciI A'CATG_T
Cuts at: 0 407 948
Size: 407 541
PfoI T'CCnGG_A
Cuts at: 0 776 948
Size: 776 172
PstI C_TGCA'G
Cuts at: 0 53 119 948
Size: 53 66 829
Fragments arranged by size:
829 66 53
SacI G_AGCT'C
Cuts at: 0 333 948
Size: 333 615
SapI GCTCTTCn'nnn_
Cuts at: 0 232 948
Size: 232 716
ScaI AGT'ACT
Cuts at: 0 24 948
Size: 24 924
SfcI C'TryA_G
Cuts at: 0 49 115 948
Size: 49 66 833
Fragments arranged by size:
833 66 49
SmaI CCC'GGG
Cuts at: 0 742 948
Size: 742 206
StyI C'CwwG_G
Cuts at: 0 611 948
Size: 611 337
TatI w'GTAC_w
Cuts at: 0 22 948
Size: 22 926
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 121 153 948
Size: 121 32 795
Fragments arranged by size:
795 121 32
XmaI C'CCGG_G
Cuts at: 0 740 948
Size: 740 208
XmnI GAAnn'nnTTC
Cuts at: 0 710 948
Size: 710 238
Enzymes that do cut:
AccI AclI AcuI AflIII AlfI AlwNI AvaI BanII
BbsI BcgI BmrI BsaBI BseRI BseYI BsgI BsiHKAI
BsmI Bsp1286I BstBI BstYI ClaI EaeI EarI EcoICRI
Eco57MI EcoRV FalI Hin4I MscI NspI PciI PfoI
PstI SacI SapI ScaI SfcI SmaI StyI TatI
TstI XmaI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AfeI AflII AgeI AhdI AleI
AloI ApaI ApaLI ApoI AscI AseI AsiSI AvrII
BaeI BamHI BanI BbeI BbvCI BciVI BclI BfrBI
BglI BglII BlpI Bme1580I BmgBI BmtI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI BsaXI BsiEI
BsiWI BsmBI BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstEII BstXI BstZ17I Bsu36I
BtgI BtgZI BtsI CspCI DraI DraIII DrdI EagI
EciI EcoNI EcoO109I EcoRI FseI FspI FspAI HaeII
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI
NotI NruI NsiI PacI PasI PflMI PmeI PmlI
PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PvuI
PvuII RsrII SacII SalI SanDI SbfI SexAI SfiI
SfoI SgfI SgrAI SmlI SnaBI SpeI SphI SrfI
SspI StuI SwaI TaqII Tth111I XbaI XcmI XhoI
ZraI