Restriction map for
pFN21AE3120
(Linear) (Six-base) MAPSORT of: KIEE3120.seq Check: 1286 from: 1 to: 897
KIEE3120.orf
With 182 enzymes: SgfI *
December 27, 2011 16:32 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 706 897
Size: 706 191
AflIII A'CryG_T
Cuts at: 0 385 897
Size: 385 512
ApaI G_GGCC'C
Cuts at: 0 435 897
Size: 435 462
AvaI C'yCGr_G
Cuts at: 0 872 897
Size: 872 25
BanII G_rGCy'C
Cuts at: 0 435 897
Size: 435 462
BciVI GTATCCnnnnn_n'
Cuts at: 0 889 897
Size: 889 8
BglII A'GATC_T
Cuts at: 0 62 563 897
Size: 62 501 334
Fragments arranged by size:
501 334 62
Bme1580I G_kGCm'C
Cuts at: 0 435 735 897
Size: 435 300 162
Fragments arranged by size:
435 300 162
BmgBI CAC'GTC
Cuts at: 0 388 897
Size: 388 509
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 633 897
Size: 633 264
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 139 541 897
Size: 139 402 356
Fragments arranged by size:
402 356 139
BsaAI yAC'GTr
Cuts at: 0 736 897
Size: 736 161
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 740 770 897
Size: 740 30 127
Fragments arranged by size:
740 127 30
BsmI GAATG_Cn'
Cuts at: 0 171 897
Size: 171 726
Bsp1286I G_dGCh'C
Cuts at: 0 435 735 897
Size: 435 300 162
Fragments arranged by size:
435 300 162
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 854 897
Size: 854 43
BsrDI GCAATG_nn'
Cuts at: 0 279 298 897
Size: 279 19 599
Fragments arranged by size:
599 279 19
BstEII G'GTnAC_C
Cuts at: 0 861 897
Size: 861 36
BstXI CCAn_nnnn'nTGG
Cuts at: 0 636 897
Size: 636 261
BstYI r'GATC_y
Cuts at: 0 62 563 897
Size: 62 501 334
Fragments arranged by size:
501 334 62
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 224 259 335 370 897
Size: 224 35 76 35 527
Fragments arranged by size:
527 224 76 35 35
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 139 541 706 897
Size: 139 402 165 191
Fragments arranged by size:
402 191 165 139
EcoNI CCTnn'n_nnAGG
Cuts at: 0 850 897
Size: 850 47
EcoO109I rG'GnC_Cy
Cuts at: 0 431 432 897
Size: 431 1 465
Fragments arranged by size:
465 431 1
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 47 79 740 772 897
Size: 47 32 661 32 125
Fragments arranged by size:
661 125 47 32 32
MspA1I CmG'CkG
Cuts at: 0 768 897
Size: 768 129
NspI r_CATG'y
Cuts at: 0 694 897
Size: 694 203
PasI CC'CwG_GG
Cuts at: 0 728 897
Size: 728 169
PflMI CCAn_nnn'nTGG
Cuts at: 0 167 897
Size: 167 730
PmlI CAC'GTG
Cuts at: 0 736 897
Size: 736 161
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 128 160 897
Size: 128 32 737
Fragments arranged by size:
737 128 32
PspOMI G'GGCC_C
Cuts at: 0 431 897
Size: 431 466
PstI C_TGCA'G
Cuts at: 0 656 897
Size: 656 241
PvuII CAG'CTG
Cuts at: 0 768 897
Size: 768 129
SexAI A'CCwGG_T
Cuts at: 0 316 897
Size: 316 581
SfcI C'TryA_G
Cuts at: 0 652 897
Size: 652 245
SmaI CCC'GGG
Cuts at: 0 874 897
Size: 874 23
SspI AAT'ATT
Cuts at: 0 216 897
Size: 216 681
StyI C'CwwG_G
Cuts at: 0 417 897
Size: 417 480
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 174 252 897
Size: 174 78 645
Fragments arranged by size:
645 174 78
XmaI C'CCGG_G
Cuts at: 0 872 897
Size: 872 25
XmnI GAAnn'nnTTC
Cuts at: 0 143 897
Size: 143 754
Enzymes that do cut:
AcuI AflIII ApaI AvaI BanII BciVI BglII Bme1580I
BmgBI BplI BpmI BsaAI BsaXI BsmI Bsp1286I BspMI
BsrDI BstEII BstXI BstYI CspCI Eco57MI EcoNI EcoO109I
Hin4I MspA1I NspI PasI PflMI PmlI PpiI PspOMI
PstI PvuII SexAI SfcI SmaI SspI StyI TaqII
XmaI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AscI
AseI AsiSI AvrII BaeI BamHI BanI BbeI BbsI
BbvCI BcgI BclI BfrBI BglI BlpI BmrI BmtI
Bpu10I BpuEI BsaI BsaBI BsaHI BsaWI BseRI BseYI
BsgI BsiEI BsiHKAI BsiWI BsmBI BspEI BspHI BsrBI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstZ17I Bsu36I
BtgI BtgZI BtsI ClaI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI EcoRI EcoRV FalI FseI
FspI FspAI HaeII HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI NsiI PacI PciI
PfoI PmeI PpuMI PshAI PsiI PspXI PsrI PvuI
RsrII SacI SacII SalI SanDI SapI SbfI ScaI
SfiI SfoI SgfI SgrAI SmlI SnaBI SpeI SphI
SrfI StuI SwaI TatI TstI Tth111I XbaI XcmI
XhoI ZraI