Restriction map for
pFN21AE3117
(Linear) (Six-base) MAPSORT of: KIEE3117.seq Check: 412 from: 1 to: 873
KIEE3117.orf
With 182 enzymes: SgfI *
March 22, 2012 16:15 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 697 873
Size: 697 176
Acc65I G'GTAC_C
Cuts at: 0 155 873
Size: 155 718
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 231 873
Size: 231 642
AhdI GACnn_n'nnGTC
Cuts at: 0 216 873
Size: 216 657
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 208 873
Size: 208 665
AlwNI CAG_nnn'CTG
Cuts at: 0 40 323 366 514 681 873
Size: 40 283 43 148 167 192
Fragments arranged by size:
283 192 167 148 43 40
ApaI G_GGCC'C
Cuts at: 0 87 873
Size: 87 786
ApaLI G'TGCA_C
Cuts at: 0 746 873
Size: 746 127
AvaI C'yCGr_G
Cuts at: 0 449 873
Size: 449 424
BanI G'GyrC_C
Cuts at: 0 155 709 873
Size: 155 554 164
Fragments arranged by size:
554 164 155
BanII G_rGCy'C
Cuts at: 0 87 456 563 873
Size: 87 369 107 310
Fragments arranged by size:
369 310 107 87
BciVI GTATCCnnnnn_n'
Cuts at: 0 421 873
Size: 421 452
BglI GCCn_nnn'nGGC
Cuts at: 0 536 873
Size: 536 337
BlpI GC'TnA_GC
Cuts at: 0 848 873
Size: 848 25
Bme1580I G_kGCm'C
Cuts at: 0 87 750 873
Size: 87 663 123
Fragments arranged by size:
663 123 87
BmgBI CAC'GTC
Cuts at: 0 585 873
Size: 585 288
BmrI ACTGGGnnnn_n'
Cuts at: 0 600 873
Size: 600 273
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 58 333 703 873
Size: 58 275 370 170
Fragments arranged by size:
370 275 170 58
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 354 873
Size: 354 519
BsaAI yAC'GTr
Cuts at: 0 255 873
Size: 255 618
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 250 397 873
Size: 250 147 476
Fragments arranged by size:
476 250 147
BsiHKAI G_wGCw'C
Cuts at: 0 66 260 551 563 638 737 750 873
Size: 66 194 291 12 75 99 13 123
Fragments arranged by size:
291 194 123 99 75 66 13 12
Bsp1286I G_dGCh'C
Cuts at: 0 66 87 260 456 551 563 638 737
Size: 66 21 173 196 95 12 75 99
Cuts at: 737 750 873
Size: 13 123
Fragments arranged by size:
196 173 123 99 95 75 66 21
13 12
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 680 697 873
Size: 680 17 176
Fragments arranged by size:
680 176 17
BsrBI CCG'CTC
Cuts at: 0 240 489 873
Size: 240 249 384
Fragments arranged by size:
384 249 240
BsrDI GCAATG_nn'
Cuts at: 0 110 142 873
Size: 110 32 731
Fragments arranged by size:
731 110 32
BsrFI r'CCGG_y
Cuts at: 0 158 535 647 873
Size: 158 377 112 226
Fragments arranged by size:
377 226 158 112
BstAPI GCAn_nnn'nTGC
Cuts at: 0 323 873
Size: 323 550
BstEII G'GTnAC_C
Cuts at: 0 277 873
Size: 277 596
BstXI CCAn_nnnn'nTGG
Cuts at: 0 117 720 808 873
Size: 117 603 88 65
Fragments arranged by size:
603 117 88 65
BstYI r'GATC_y
Cuts at: 0 362 873
Size: 362 511
Bsu36I CC'TnA_GG
Cuts at: 0 664 873
Size: 664 209
BtgI C'CryG_G
Cuts at: 0 10 873
Size: 10 863
BtsI GCAGTG_nn'
Cuts at: 0 515 873
Size: 515 358
DraIII CAC_nnn'GTG
Cuts at: 0 555 873
Size: 555 318
EaeI y'GGCC_r
Cuts at: 0 533 649 780 873
Size: 533 116 131 93
Fragments arranged by size:
533 131 116 93
EarI CTCTTCn'nnn_
Cuts at: 0 13 742 873
Size: 13 729 131
Fragments arranged by size:
729 131 13
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 517 873
Size: 517 356
EcoICRI GAG'CTC
Cuts at: 0 561 873
Size: 561 312
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 58 231 333 703 873
Size: 58 173 102 370 170
Fragments arranged by size:
370 173 170 102 58
EcoNI CCTnn'n_nnAGG
Cuts at: 0 353 873
Size: 353 520
EcoO109I rG'GnC_Cy
Cuts at: 0 37 83 346 390 492 669 873
Size: 37 46 263 44 102 177 204
Fragments arranged by size:
263 204 177 102 46 44 37
FspI TGC'GCA
Cuts at: 0 184 873
Size: 184 689
FspAI rTGC'GCAy
Cuts at: 0 184 873
Size: 184 689
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 202 234 873
Size: 202 32 639
Fragments arranged by size:
639 202 32
KpnI G_GTAC'C
Cuts at: 0 159 873
Size: 159 714
MspA1I CmG'CkG
Cuts at: 0 323 615 678 791 873
Size: 323 292 63 113 82
Fragments arranged by size:
323 292 113 82 63
NaeI GCC'GGC
Cuts at: 0 537 873
Size: 537 336
NcoI C'CATG_G
Cuts at: 0 10 873
Size: 10 863
NgoMIV G'CCGG_C
Cuts at: 0 535 873
Size: 535 338
PasI CC'CwG_GG
Cuts at: 0 80 463 873
Size: 80 383 410
Fragments arranged by size:
410 383 80
PpuMI rG'GwC_Cy
Cuts at: 0 492 669 873
Size: 492 177 204
Fragments arranged by size:
492 204 177
PshAI GACnn'nnGTC
Cuts at: 0 284 873
Size: 284 589
PspOMI G'GGCC_C
Cuts at: 0 83 873
Size: 83 790
PstI C_TGCA'G
Cuts at: 0 677 873
Size: 677 196
PvuII CAG'CTG
Cuts at: 0 615 678 873
Size: 615 63 195
Fragments arranged by size:
615 195 63
SacI G_AGCT'C
Cuts at: 0 563 873
Size: 563 310
SanDI GG'GwC_CC
Cuts at: 0 492 873
Size: 492 381
SapI GCTCTTCn'nnn_
Cuts at: 0 742 873
Size: 742 131
ScaI AGT'ACT
Cuts at: 0 331 873
Size: 331 542
SexAI A'CCwGG_T
Cuts at: 0 629 873
Size: 629 244
SfcI C'TryA_G
Cuts at: 0 673 873
Size: 673 200
SmlI C'TyrA_G
Cuts at: 0 333 873
Size: 333 540
StyI C'CwwG_G
Cuts at: 0 10 873
Size: 10 863
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 277 619 873
Size: 277 342 254
Fragments arranged by size:
342 277 254
TatI w'GTAC_w
Cuts at: 0 329 873
Size: 329 544
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 798 830 873
Size: 798 32 43
Fragments arranged by size:
798 43 32
Tth111I GACn'n_nGTC
Cuts at: 0 99 873
Size: 99 774
Enzymes that do cut:
AarI Acc65I AcuI AhdI AlfI AlwNI ApaI ApaLI
AvaI BanI BanII BciVI BglI BlpI Bme1580I BmgBI
BmrI BpmI BpuEI BsaAI BseRI BsiHKAI Bsp1286I BspMI
BsrBI BsrDI BsrFI BstAPI BstEII BstXI BstYI Bsu36I
BtgI BtsI DraIII EaeI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I FspI FspAI Hin4I KpnI MspA1I NaeI
NcoI NgoMIV PasI PpuMI PshAI PspOMI PstI PvuII
SacI SanDI SapI ScaI SexAI SfcI SmlI StyI
TaqII TatI TstI Tth111I
Enzymes that do not cut:
AatII AccI AclI AfeI AflII AflIII AgeI AleI
AloI ApoI AscI AseI AsiSI AvrII BaeI BamHI
BbeI BbsI BbvCI BcgI BclI BfrBI BglII BmtI
BplI Bpu10I BsaI BsaBI BsaHI BsaWI BsaXI BseYI
BsgI BsiEI BsiWI BsmI BsmBI BspEI BspHI BsrGI
BssHII BssSI BstBI BstZ17I BtgZI ClaI CspCI DraI
DrdI EagI EcoRI EcoRV FalI FseI HaeII HincII
HindIII HpaI KasI MfeI MluI MmeI MscI MslI
NarI NdeI NheI NotI NruI NsiI NspI PacI
PciI PflMI PfoI PmeI PmlI PpiI PsiI PspXI
PsrI PvuI RsrII SacII SalI SbfI SfiI SfoI
SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI
StuI SwaI XbaI XcmI XhoI XmaI XmnI ZraI