Restriction map for
pFN21AE3113
(Linear) (Six-base) MAPSORT of: KIEE3113.seq Check: 7789 from: 1 to: 858
KIEE3113.orf
With 182 enzymes: SgfI *
December 27, 2011 16:32 ..
AccI GT'mk_AC
Cuts at: 0 368 858
Size: 368 490
Acc65I G'GTAC_C
Cuts at: 0 197 858
Size: 197 661
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 324 858
Size: 324 534
AleI CACnn'nnGTG
Cuts at: 0 820 858
Size: 820 38
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 124 156 858
Size: 124 32 702
Fragments arranged by size:
702 124 32
AlwNI CAG_nnn'CTG
Cuts at: 0 799 858
Size: 799 59
ApaI G_GGCC'C
Cuts at: 0 490 534 858
Size: 490 44 324
Fragments arranged by size:
490 324 44
ApoI r'AATT_y
Cuts at: 0 686 858
Size: 686 172
AvaI C'yCGr_G
Cuts at: 0 15 33 81 858
Size: 15 18 48 777
Fragments arranged by size:
777 48 18 15
BanI G'GyrC_C
Cuts at: 0 6 197 858
Size: 6 191 661
Fragments arranged by size:
661 191 6
BanII G_rGCy'C
Cuts at: 0 82 88 490 534 858
Size: 82 6 402 44 324
Fragments arranged by size:
402 324 82 44 6
BbeI G_GCGC'C
Cuts at: 0 10 858
Size: 10 848
BfrBI ATG'CAT
Cuts at: 0 466 858
Size: 466 392
BglI GCCn_nnn'nGGC
Cuts at: 0 19 100 212 494 858
Size: 19 81 112 282 364
Fragments arranged by size:
364 282 112 81 19
Bme1580I G_kGCm'C
Cuts at: 0 490 499 534 858
Size: 490 9 35 324
Fragments arranged by size:
490 324 35 9
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 151 172 342 858
Size: 151 21 170 516
Fragments arranged by size:
516 170 151 21
BsaAI yAC'GTr
Cuts at: 0 322 858
Size: 322 536
BsaHI Gr'CG_yC
Cuts at: 0 7 858
Size: 7 851
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 124 154 858
Size: 124 30 704
Fragments arranged by size:
704 124 30
BseYI C'CCAG_C
Cuts at: 0 22 118 301 564 584 789 858
Size: 22 96 183 263 20 205 69
Fragments arranged by size:
263 205 183 96 69 22 20
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 193 858
Size: 193 665
BsiHKAI G_wGCw'C
Cuts at: 0 421 858
Size: 421 437
Bsp1286I G_dGCh'C
Cuts at: 0 82 88 421 490 499 534 858
Size: 82 6 333 69 9 35 324
Fragments arranged by size:
333 324 82 69 35 9 6
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 305 858
Size: 305 553
BsrDI GCAATG_nn'
Cuts at: 0 407 559 858
Size: 407 152 299
Fragments arranged by size:
407 299 152
BsrFI r'CCGG_y
Cuts at: 0 9 110 858
Size: 9 101 748
Fragments arranged by size:
748 101 9
BstAPI GCAn_nnn'nTGC
Cuts at: 0 799 858
Size: 799 59
BtgI C'CryG_G
Cuts at: 0 95 858
Size: 95 763
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 230 858
Size: 230 628
BtsI GCAGTG_nn'
Cuts at: 0 512 547 576 858
Size: 512 35 29 282
Fragments arranged by size:
512 282 35 29
EarI CTCTTCn'nnn_
Cuts at: 0 260 858
Size: 260 598
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 252 858
Size: 252 606
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 151 172 324 342 858
Size: 151 21 152 18 516
Fragments arranged by size:
516 152 151 21 18
EcoO109I rG'GnC_Cy
Cuts at: 0 486 487 531 858
Size: 486 1 44 327
Fragments arranged by size:
486 327 44 1
EcoRI G'AATT_C
Cuts at: 0 686 858
Size: 686 172
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 402 798 858
Size: 402 396 60
Fragments arranged by size:
402 396 60
FspI TGC'GCA
Cuts at: 0 277 858
Size: 277 581
HaeII r_GCGC'y
Cuts at: 0 10 44 858
Size: 10 34 814
Fragments arranged by size:
814 34 10
KasI G'GCGC_C
Cuts at: 0 6 858
Size: 6 852
KpnI G_GTAC'C
Cuts at: 0 201 858
Size: 201 657
MslI CAynn'nnrTG
Cuts at: 0 215 820 858
Size: 215 605 38
Fragments arranged by size:
605 215 38
MspA1I CmG'CkG
Cuts at: 0 97 203 858
Size: 97 106 655
Fragments arranged by size:
655 106 97
NaeI GCC'GGC
Cuts at: 0 11 112 858
Size: 11 101 746
Fragments arranged by size:
746 101 11
NarI GG'CG_CC
Cuts at: 0 7 858
Size: 7 851
NgoMIV G'CCGG_C
Cuts at: 0 9 110 858
Size: 9 101 748
Fragments arranged by size:
748 101 9
NsiI A_TGCA'T
Cuts at: 0 468 858
Size: 468 390
NspI r_CATG'y
Cuts at: 0 337 858
Size: 337 521
PfoI T'CCnGG_A
Cuts at: 0 358 858
Size: 358 500
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 124 156 858
Size: 124 32 702
Fragments arranged by size:
702 124 32
PspOMI G'GGCC_C
Cuts at: 0 486 530 858
Size: 486 44 328
Fragments arranged by size:
486 328 44
SacII CC_GC'GG
Cuts at: 0 98 858
Size: 98 760
SfcI C'TryA_G
Cuts at: 0 48 858
Size: 48 810
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 100 858
Size: 100 758
SfoI GGC'GCC
Cuts at: 0 8 858
Size: 8 850
SmaI CCC'GGG
Cuts at: 0 17 858
Size: 17 841
StyI C'CwwG_G
Cuts at: 0 546 837 858
Size: 546 291 21
Fragments arranged by size:
546 291 21
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 274 306 463 495 858
Size: 274 32 157 32 363
Fragments arranged by size:
363 274 157 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 126 858
Size: 126 732
XmaI C'CCGG_G
Cuts at: 0 15 858
Size: 15 843
XmnI GAAnn'nnTTC
Cuts at: 0 686 858
Size: 686 172
Enzymes that do cut:
AccI Acc65I AcuI AleI AloI AlwNI ApaI ApoI
AvaI BanI BanII BbeI BfrBI BglI Bme1580I BpmI
BsaAI BsaHI BsaXI BseYI BsgI BsiHKAI Bsp1286I BspMI
BsrDI BsrFI BstAPI BtgI BtgZI BtsI EarI EciI
Eco57MI EcoO109I EcoRI FalI FspI HaeII KasI KpnI
MslI MspA1I NaeI NarI NgoMIV NsiI NspI PfoI
PpiI PspOMI SacII SfcI SfiI SfoI SmaI StyI
TstI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AclI AfeI AflII AflIII AgeI AhdI
AlfI ApaLI AscI AseI AsiSI AvrII BaeI BamHI
BbsI BbvCI BcgI BciVI BclI BglII BlpI BmgBI
BmrI BmtI BplI Bpu10I BpuEI BsaI BsaBI BsaWI
BseRI BsiEI BsiWI BsmI BsmBI BspEI BspHI BsrBI
BsrGI BssHII BssSI BstBI BstEII BstXI BstYI BstZ17I
Bsu36I ClaI CspCI DraI DraIII DrdI EaeI EagI
EcoICRI EcoNI EcoRV FseI FspAI Hin4I HincII HindIII
HpaI MfeI MluI MmeI MscI NcoI NdeI NheI
NotI NruI PacI PasI PciI PflMI PmeI PmlI
PpuMI PshAI PsiI PspXI PsrI PstI PvuI PvuII
RsrII SacI SalI SanDI SapI SbfI ScaI SexAI
SgfI SgrAI SmlI SnaBI SpeI SphI SrfI SspI
StuI SwaI TaqII TatI Tth111I XbaI XhoI ZraI